LOCUS       NZ_CP058144             1917 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain RHBSTW-00138 plasmid
            pRHBSTW-00138_14, complete sequence.
ACCESSION   NZ_CP058144
VERSION     NZ_CP058144.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148509
            Assembly: GCF_013624775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00138
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0293412188064x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 53 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 27 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00138"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1463165"
                     /plasmid="pRHBSTW-00138_14"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            551..787
                     /locus_tag="HV092_RS28020"
                     /old_locus_tag="HV092_28025"
     CDS             551..787
                     /locus_tag="HV092_RS28020"
                     /old_locus_tag="HV092_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1047..1349)
                     /locus_tag="HV092_RS28025"
                     /old_locus_tag="HV092_28030"
     CDS             complement(1047..1349)
                     /locus_tag="HV092_RS28025"
                     /old_locus_tag="HV092_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_181352640.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIVAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     Typing          213..386
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2..234)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.34"
                     /coverage="81.0"
                     /evalue="1.68e-88"
     Typing          complement(1454..1917)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.74"
                     /coverage="93.0"
                     /evalue="8.31e-121"
ORIGIN
        1 tatctttaag ccgcatcggg tttcgccacc actgatttga gcgtcagatt tcatgatgtt
       61 tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc ggccttatct cttccctgtt
      121 aagctccttc ctggtatctc ccaggaaatt tccgccccgc ctgtaagccg gcaccgctcg
      181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaat atatcctgta
      241 ctacatatcc tgctgacgcg ctggtgccgc cttttttctc ctgctacatg aagcacttca
      301 ctgatttcca catccgtgcc aacatagtca gccagtatac actccgctag cgctacgtga
      361 ctggttcagg gctgcgcccc gaaacccgct aaaagctaat gacgcgcctg cggcttgtcc
      421 aacgcctcac ggcaccggat taaaatccgc tcccgttcac cgtttctgac tgcctttcac
      481 cgtcgctggc gcttcatcgt ttctactgtg tctggcagac tgtgctgatt tattctgact
      541 ggagaacgtt atggccagac tgacaaactt gacgcctgca gagaaaaaat ttcttgatga
      601 tgctgttgcc gcagctgaaa gagcgtcagg taaaaagcta aatcagccta accggcacat
      661 cgtcctgaac agagcgcgtg cgcaaattga gttgcagcgt tacgccgacc gccagcgtgc
      721 attacgtgag gatgagcggc agcagtccga ttttgcctgg tcacgtccgc gcgcgccgcg
      781 ccgctgattt tggcttttgg cggtttagtg gtgctgacgc tgcgaaatca cctgccattc
      841 acctgcgtgc atagccatgc agtcgcgcag aaatcatcag gggcgttttt ccgggatact
      901 ggtgaggaaa ggtgctggaa acggccctga tgcagcagga ttgcgctgag ggcgcctgcg
      961 ggggtgcggg caagggatgt tatgtttaat cggtctgacc gtcaggtaca aaaaaaagcc
     1021 cttagggggc ttttttttgc tatgagttag ccatattttc gtgcgaattt ttcatcggaa
     1081 tcgcatagat aaataataaa ctcaataaac gcgatgatcg cgggtatgaa tgtccagcaa
     1141 aaaattagat acaggaaccc ctgtcctatt tttcctaagt aaaatttatg tgcgccaaat
     1201 ccaccaagga agaatgctaa tagcgcggct acgattctac ttttttcacc tgaacctgaa
     1261 gaggcgtttg tggctccgca gtgcgggcat gactttgctg tttcgtgtat ttccttgcca
     1321 catcctcggc agtaaaccat tgaactcatt ttttatcctc gagcttattt taattgggat
     1381 cataatatcc gatgttttat aacgatgtgt atcctatgtg ctcggattgg atagatttcc
     1441 attacggtga aggatcttct tgagatcctt tctgtggcgt cgtaatctct tgctctgtaa
     1501 acgaaaaaac cgccctgcaa ggcggttttt cgaaggttct cagagcagca actctttgaa
     1561 ccgtggtaac tggcttacga ggagcgcagt aacaaaatct gttctttcag tttagcctta
     1621 acaggcgcat aacttcaaga ctaactcctc taaatcagtt accagtggct gctgccagtg
     1681 gcgataagtc gtgtctttcc gggttggact caagatgata gttaccggat aaggcgcagc
     1741 agtcggactg aacggggggt tcgtgcatac agtccatcca ggagcgaact gccttcgcgg
     1801 aactgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta
     1861 aaccgaaagg caggaatgcg ggggagcacg agggagccac cagggggaaa cgcctgg
//
