LOCUS       NZ_CP058143             1934 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain RHBSTW-00138 plasmid
            pRHBSTW-00138_13, complete sequence.
ACCESSION   NZ_CP058143
VERSION     NZ_CP058143.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148509
            Assembly: GCF_013624775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00138
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0293412188064x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 53 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 27 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058143.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00138"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1463165"
                     /plasmid="pRHBSTW-00138_13"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(1831..1934,1..613)
                     /locus_tag="HV092_RS28015"
                     /old_locus_tag="HV092_28020"
     CDS             join(1831..1934,1..613)
                     /locus_tag="HV092_RS28015"
                     /old_locus_tag="HV092_28020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_249415441.1"
                     /translation="MWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETLNRMN
                     MAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHDRWVE
                     LWRECLRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPADMVADP
                     EWFLELTKQTHKRRFVATGGALKDILKLDQETDADMVIGDDIPEGDDDGSRIAFEWKTE
                     SKKYRRSPSKDKAELD"
                     /name="protein rep"
     Typing          complement(340..528)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 ggaattgggt gagacactta accgaatgaa catggccttc cagcgactta aggatcgcaa
       61 agagtttcga cctgttcagg gctggatccg caccacggaa gttacacgtg gaagtgacgg
      121 tagtgcgcac ccgcactttc acactctgat gatggttcct ccctcaatgt tcacgcgaga
      181 ttacgttaaa catgaccgct gggtagagct ttggcgagag tgtttgcgtg tgaattacga
      241 tccgaatgtt gatgttcgag cagtaaaacc ccgcaagcct aaggacggag aatcattggc
      301 ttctgcaact gcggaacttg tacggggcgc agtggctgaa accctgaaat attccacgaa
      361 gccggctgac atggttgctg atcctgaatg gttcctcgaa ttgactaagc agacacataa
      421 gcgccgattt gtggctaccg gtggcgcact caaagacata ctgaaactcg atcaggaaac
      481 tgatgccgat atggtaatcg gggatgatat tcccgaaggc gatgatgatg gttcacgcat
      541 tgcttttgag tggaaaaccg aatccaaaaa gtaccgtcgc tcaccgtcaa aagataaagc
      601 tgaattagac tgacagggtg tcggggcgca gccctgacca cgacgttttt gagaggacgc
      661 cgcttgtcgg cggtactcca aaaacacatc ttgtcctatt ggttctactc cgatgcagga
      721 tagggctaac gccgcaaagt gacgtcactc aattactgag ttcggtttcc gaactcagtt
      781 gggacaagat gtgtttttgg tacaccgcca tcacgatgtc ggtgccaaaa acgattcgtc
      841 agggctgcgc cccgacaccc tgccaacacc tcacgcagtc agattaaaat ctgccagcgc
      901 tcgccgtttc cgaaatcgtc aaactctaaa tcgtggggtc ccgctggctt gccagtgggt
      961 tccgatttag tgattgacga cattgctgat tgaaaaattg gctgaggtgg ttggattcac
     1021 gaagtgaggc caaccacctg gaagacggtt tttaattgtc tggtaccgga tacaaccaga
     1081 acgaatggag ttggttctgt cgtgatgcga aaaagtatgg gttcgcaaat cacagactaa
     1141 atagatttac ccaattccat tcgagtgagg ttgttatgtt ttttgaggtt cttaatcatg
     1201 ttgatagtgc gttgagcgtt gctgtttcgc tcctggcgtt gtggcagttt ttggtggcgg
     1261 gtgggcgttg aatggctggt tcgatccgcg cgcgatcctt gtatttatac ttaagggata
     1321 actctggcga taaaaaatgg catcaagcct tgcgtaccaa ggcttcgggg cttatttgcg
     1381 ttgtagaagg ttttggaagt attctgttgg gagtggtcgc cttaaaagcg acccccgcaa
     1441 cgacggccat cgttcggggg tgtgaccacc tgcagaaatt cgattcgaag cgacaaccaa
     1501 caggtgatac ggcaatgata acacgtcaaa tgagccatcg cccagcatgg gacggtgtgc
     1561 atgcaaaacc ctaaactcca gaacctcacg gattactccc cctcagacgc gccctgggac
     1621 gctcacaaaa gcgtttctga cgacgtaggt gggatttacc ttctcgcagc cgaatatgag
     1681 cgctacgggg cgcgtatggc ttcctgtggc ggtttactga ggtttggttg gtctaccctc
     1741 aaagaaactg gagaaactcg ccttaggcta cgtgaggcgc atttctgccg tgtccggcac
     1801 tgtcctgtct gccagtggag gcgatccctg atgtggcaag cccgttttta tcagtccctg
     1861 ccgcgtattg ttgcggatta tccagatgca cgctggatgt tcctgaccct cacagtgcga
     1921 aactgcgcga tcgg
//
