LOCUS       NZ_CP058141             2349 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain RHBSTW-00138 plasmid
            pRHBSTW-00138_11, complete sequence.
ACCESSION   NZ_CP058141
VERSION     NZ_CP058141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148509
            Assembly: GCF_013624775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00138
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0293412188064x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 53 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 27 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00138"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1463165"
                     /plasmid="pRHBSTW-00138_11"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            460..1206
                     /locus_tag="HV092_RS27995"
                     /old_locus_tag="HV092_28000"
     CDS             460..1206
                     /locus_tag="HV092_RS27995"
                     /old_locus_tag="HV092_28000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_052684122.1"
                     /translation="MDLGNILNESLSIDYEKLDLFLEKYDLTPEQKVAVYEFHAKAYKK
                     NKTLVISETKENKFKSISEGVEYVHLFPKNLKILIKKYGLNTNELLVLTEIMESMLSHG
                     NLLINFSQKALCELTGINKSTMCKTFKTLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFM
                     QFRDHFLNSISYKLDDEEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDNFE
                     KEISKEWKEKFKDEEENFEFGFESEI"
                     /name="replication/maintenance protein RepL"
     gene            1574..2302
                     /locus_tag="HV092_RS28000"
                     /old_locus_tag="HV092_28005"
     CDS             1574..2302
                     /locus_tag="HV092_RS28000"
                     /old_locus_tag="HV092_28005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_097725005.1"
                     /translation="MGNSKRNIKKLNDNFREDILDYAFAHNLKCANALAILYATGCRPD
                     ELQTGVTVNYDSKKNEIEFRIIGSKLNRRMRRGIGVRKIKVKINNENARFFKNIVDKFI
                     ENPMSYDHKIKIESAKAFSGYITKISKKLWPRKTYHASAYSFRHAKATELKNSDYDKIE
                     IAQIMGHASVRSQQSYGRKSKKSKGGFDDIADVETNVKPRGGDRLLRFKIANKNKAAAK
                     IADTSTPSSPPPAPVRRFKM"
                     /name="site-specific integrase"
     Typing          15..198
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.297"
                     /coverage="100.0"
                     /evalue="5.88e-83"
ORIGIN
        1 ccgtccggca ggacggtttt atagcaacat cctccatagc gaagcagaag cccggaacgg
       61 gtaacctgga gggttttccc ccgtggggga ttgatctgtt acttgaaaac caatgatctt
      121 aaaaaccatc tcaaaagttg aaaatttcac ccccttagtg ttcttaaaat tcttagatgt
      181 tcttaggagt taaaaaacta ctctctaacc attgatatta ctcatttttt aaaaatggca
      241 gttgtcaaaa acttcaaccg tagttgtcaa attcgtcaac tccagttgtc aaattcgtca
      301 actgaggttg tcaaatccga caactctagc cagtttccaa gtagaaacta cagtttcaaa
      361 actgaaactt tttctacctt ttgcaactta atctattgac tagtctttta taaatgttaa
      421 aacatatata tagaaataaa taaaaaagag gaggtttcta tggatttagg aaatatatta
      481 aatgagagtt taagtattga ttacgaaaaa ttagatttgt ttttggaaaa atatgattta
      541 acaccagaac aaaaagttgc agtttatgaa tttcacgcaa aagcttataa aaaaaataaa
      601 actttagtta tttctgaaac aaaagaaaat aaatttaaat ctatttccga aggtgttgaa
      661 tacgtgcatt tattcccaaa aaatttaaaa attttaatta aaaaatatgg tttaaataca
      721 aacgaattat tggttttaac ggaaataatg gagtcaatgc tttcacacgg aaatttatta
      781 attaattttt cgcaaaaggc actttgcgaa ttaacaggaa ttaataaatc tacaatgtgt
      841 aaaacattta aaaccctcaa acaaaagcag tgtttaattg agaaaaacgg acatatttat
      901 ttaaattctg taatatttat gaaaggttta cctcataaat tgtttatgca atttagagat
      961 cattttttaa attctatctc atataaatta gatgatgaag aagaatttga aaaagtcttc
     1021 gacgataatt ttattaaagc atatgaaaaa aatctcaaag agattaaaaa gaaaaagcaa
     1081 caaataaaag aaaagaaaat atctaaagca ttagacaatt ttgaaaaaga aatctcgaaa
     1141 gaatggaaag aaaagtttaa agacgaagag gaaaatttcg aatttggttt tgaatcggaa
     1201 atataaaacc gccctcgccg ggcaggccaa tcccttactg aaatagaata aattctattc
     1261 cactaaggga ttttttttat ccattaattc tccacatttc aaatattgac agcattttcc
     1321 acccggttat aacagtacta taagtagttg tttctacccc gattttgggg tggaacaaca
     1381 aggcatttta gggatagagc aaagcgaaag ccataaattg ccacccccaa ccgggggtcg
     1441 ttgttcgatt tgagcgatag cgaaaaattg aacataaggg gggagggttt gggttttacg
     1501 gtatttcaaa tttgagcaaa gcgaattttt tgaaatttcc ggttctttta atttgcaatg
     1561 aggaaaaatc aatatgggta attcaaaaag aaatataaaa aaactaaatg ataattttag
     1621 agaggatatt ttagattatg cgttcgcgca caatctaaaa tgtgctaacg cacttgctat
     1681 tttatatgcg acgggttgcc gtccggacga actccaaacc ggagttacgg taaactatga
     1741 cagcaaaaaa aatgaaattg aatttagaat aattggatca aaactaaata gaagaatgag
     1801 aagaggcata ggggttagaa aaataaaagt aaaaatcaat aatgaaaatg ccagattttt
     1861 taaaaacatt gttgataaat ttattgaaaa cccaatgtca tatgatcaca aaataaaaat
     1921 tgaaagtgcc aaagcatttt ccgggtacat aacaaaaata tcgaaaaagc tatggcccag
     1981 gaaaacctat catgcttctg catattcttt tagacatgca aaagcaacgg aattgaaaaa
     2041 ttccgattat gataaaatcg aaatagctca gattatgggc catgcctcag ttagatctca
     2101 gcagagttac ggaagaaaga gcaaaaaaag caaaggtgga tttgatgaca tcgcagatgt
     2161 cgaaaccaat gttaaacccc gtggcggtga tagattattg agatttaaga tcgcaaataa
     2221 aaacaaagca gcggcaaaaa ttgccgatac ttccaccccc agcagtcctc caccggctcc
     2281 cgttcgtcgc ttcaaaatgt gaaccgtgag cagttcagga ggtcccctcc tggactgtga
     2341 agggttggc
//
