LOCUS       NZ_CP058140             3360 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain RHBSTW-00138 plasmid
            pRHBSTW-00138_10, complete sequence.
ACCESSION   NZ_CP058140
VERSION     NZ_CP058140.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148509
            Assembly: GCF_013624775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00138
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0293412188064x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 53 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 27 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00138"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1463165"
                     /plasmid="pRHBSTW-00138_10"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3256..3360,1..282))
                     /locus_tag="HV092_RS27980"
                     /old_locus_tag="HV092_27985"
     CDS             complement(join(3256..3360,1..282))
                     /locus_tag="HV092_RS27980"
                     /old_locus_tag="HV092_27985"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            449..1384
                     /locus_tag="HV092_RS27985"
                     /old_locus_tag="HV092_27990"
     CDS             449..1384
                     /locus_tag="HV092_RS27985"
                     /old_locus_tag="HV092_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            1387..1917
                     /locus_tag="HV092_RS27990"
                     /old_locus_tag="HV092_27995"
     CDS             1387..1917
                     /locus_tag="HV092_RS27990"
                     /old_locus_tag="HV092_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     Typing          450..1381
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatcatcaga ccagccgcca gaaacagtct gtgattgcgt tccctgcggt ttttctgccc
       61 tgaaatccgc gcctgaaccg cctttttctg tgcggcatcc cgttcctgca acgcgttttt
      121 ttcagcggtt gccagaacgt ggagcagtcg tctttcagtc tcactgagcg aagcttcatc
      181 atccagcaga tcgataatct gccgttgccg tgccgttttt tctcccagct ttctgatgaa
      241 ggctatgcgc tttttaagcc attcgacgtt actgatccgc atcgtttaac ttccccatcc
      301 cgatatttcc gacggggaaa gtatataact tgcggtcaga agtcgcatga cttatcttta
      361 ctcataattc tttaaattag agtaaagcgc acttatatgt ttctccctgc gggcagaaac
      421 agtgcgccct gccgggggct gacacaacat ggcaacgtat catctcagcg tgaaatttgg
      481 cggcaagggt aaagcccttg ctcatgccag ttacattacg cgggaagata agttcagcca
      541 gcgccaggat ctcgagcaca ccgaacacgg caatatgccg gaatgggcga gagatgaacc
      601 cgcccatttc tggcaggctg cggatgcgtt cgaacgtgcc aacggttcaa cgtacaggga
      661 aattgagatc gctctacccc gcgagctgaa tgaagcgcag cggctggcgc ttgtccggga
      721 ttttgtgaag caggaaacgg gcgacaaaca tgcatggacg tttgcgatcc acaatccgaa
      781 agccagcatt gacggcggcg aacagccgca cgcccatatc atgatgagcc agcgcgtcaa
      841 tgacggtatt gagcgcagtc ccgaacagta tttcaggcgc tataacgcca aatttccggc
      901 gcgtggcggc gcgcgcaagg actcagggca taatttaacc cctacagaac aaaaacaggc
      961 actgaaagcc ctgcgggagc gctgggagcg tacgcacaac gcgcatatga aacgatatgg
     1021 ctttgagcag ggttttattg atagccggtc actgaaaaca cagggtatcg accggaaacc
     1081 ggaacagcat ctcggttttg aagcagccgg acgactggac gacagccaga ggcaggatat
     1141 taaagacagc cggacgtttt cagtcgtgga tgtgcctgaa caggcgaaac atatcgacca
     1201 cagcgaccgt agctggatga atgccagcgg tatcgaggta aaccggaagg aactggtcag
     1261 atctctgacg catgatatct cgcagcagta cgggcgggag atggctgcac gggtcacaaa
     1321 acagggaaat gttgaaatag agcgcgtgaa atccctgtca cgcggatttt ccataagcag
     1381 gtaactatgg cgagcaccga agaaaaactt ctcacactcc tgctgtcggc ggagcatctg
     1441 aaagaggccg caaaagagca gcagaaagcc gtggcggatt cgctggcggt ggcaagggaa
     1501 accctgaaag gctaccggga aatatcggac agtgtggcac agcaggtaag ggatgaagtc
     1561 cggcaggaag tgcagaaaat ggatgttgcg gggctgattg gcgagcgtat cagcgctgat
     1621 tttcagcgtc tggaaaaatc cgtcgggaac atgaaaatca atgcggacgc gctggacaag
     1681 gaaatttcca ttatccgcag taacatcatc gcggagtaca acacgctgcg gggttacagc
     1741 tggaagtggt ttgctggggt tgcggttgcc tttgtcgtac tgatatttgc cacaagctgg
     1801 tttgtgaaaa cggaactaca ggacaattac aacgcgtctg ctgccgtcta tcagaaagtg
     1861 gcagaccttg aaaagctgct gacaacggaa cagaaaccgg aaaagaaaaa gaactgacgc
     1921 gctgcgcttg tccaacgcct cacgttcgca caataaattg cgcacccgtt caccgttttc
     1981 agccgagttt ggggcgtctt ttgagacttc cggcctgaat tcaggccgga agttctcgcc
     2041 agtcagtcag gtgcatggtt atgcatgggg ctgaaaatca cggtatgcga ttctgagcgg
     2101 ttttcggtga gaagccgggg gtttttatgg ctcaggactt aaaaccgccc tgagagcctc
     2161 tgaggcggtg cacgcaacgg atgttatggt aaatccggtg gctttgacgt gcctgccgtt
     2221 gtgtaccgtc gtgagtgaaa ataattatat tgttcgaaga acagcgcttt ttggccggag
     2281 aaaagcagtt ctgcaaacga tggtatatcg atatgagttc ttttggcatt aatccaggca
     2341 agacgccggg caataagcgt aatattccgg atgttactta tttccatttc tgtcaacagc
     2401 atcccccccc acgccggaga ataaaaaagc cgcctgttcc atcggcaagc cctacatcaa
     2461 gggaaactac gtcttacgat tcgttttgaa ccgggacgct ttgttgctgc gaatcactaa
     2521 ccagataatc cacgttttgt gagtgggttt aggttaaggc ttcaatagaa caataatagc
     2581 gttaagtgat tagggcaacc tgtgattgct aaaatgatga agtcttcaca tcatttcggt
     2641 gaaggcgata tgtgttacac cacaacccgg atttgagagt cgcaaaccca tattagggca
     2701 aagaaagcct aatgcccccg tggtgggtac accagaaaac ggggagcgaa gcgaccagca
     2761 tcaggccgtt cgtatgctgc ttcgattttg tgggtgttgc gccagacctt ggcaatcacg
     2821 cttcaccgca accgctgcct tcactctcac tttatcaggt acgttattgg ttaacgtgag
     2881 accgggtaga gccagcttct ttcccgagct ggtcaggttc ccaccgcttg cctgtttatc
     2941 ctgccggagt ccggggcgta gacctgagag tcaaggtgca gatatgggtt taatgtcgtt
     3001 gaatggtatt aagctggtag tgacattcct agccagccct gctaggattt gattgtgacg
     3061 tctgatcctg gcaggtatct gcaagaatta gagtactcaa gccccgtgat tctgtcacgg
     3121 ggcttttgta catctggagc aagaaaattc atgctcctat ccgaaaatcc ctaccgctcg
     3181 ccgcagccga acgaccgagc gacgcgagtc agtgagcgag gaagcggcat ttctccggcc
     3241 ttgcagaatg cacgttcacg ccgatgtttc cagatcgtag cgtacccgtt tcagcgcagt
     3301 cagtaatttt tcgtggtcaa aattcaactc acccgttttt gtatcaacca gtcccgcgtc
//
