LOCUS       NZ_CP058139             3847 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain RHBSTW-00138 plasmid
            pRHBSTW-00138_9, complete sequence.
ACCESSION   NZ_CP058139
VERSION     NZ_CP058139.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148509
            Assembly: GCF_013624775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00138
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0293412188064x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 53 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 27 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058139.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00138"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1463165"
                     /plasmid="pRHBSTW-00138_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3832..3847,1..221)
                     /locus_tag="HV092_RS27960"
                     /old_locus_tag="HV092_27965"
     CDS             join(3832..3847,1..221)
                     /locus_tag="HV092_RS27960"
                     /old_locus_tag="HV092_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            <655..873
                     /locus_tag="HV092_RS27965"
                     /old_locus_tag="HV092_27970"
                     /pseudo=""
     CDS             <655..873
                     /locus_tag="HV092_RS27965"
                     /old_locus_tag="HV092_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014719223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /name="DUF4442 domain-containing protein"
     gene            complement(933..2129)
                     /gene="fabV"
                     /locus_tag="HV092_RS27970"
                     /old_locus_tag="HV092_27975"
     CDS             complement(933..2129)
                     /gene="fabV"
                     /locus_tag="HV092_RS27970"
                     /old_locus_tag="HV092_27975"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011061617.1"
                     /GO_function="GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="WP_057175007.1"
                     /translation="MVIKPRVRGFICVTTHPVGCEANVKQQIDYVTQQGAIDAGPKNVL
                     VLGASTGYGLAARITAAFGCGANTLGVFFERGSVDGKLGTAGWYNTAAFHKFAEAKGLY
                     AKSINGDAFSDAVKAETIETIKRDLGKIDLVVYSLAAPRRTHPKTGEVLTSTLKPLGKS
                     VTLRGVDTDKEIVKETTLQPATQEEIDGTVAVMGGEDWQMWIDALHEAGVLAEGAKTTA
                     FTYLGEKLTHDIYWNGSIGEAKKDLDHKVIGMREQLADLGGDARVSVLKAVVTQASSAI
                     PIMPLYLSLLFKVMKEQGSHEGCIEQVYGLYKDSLYNSEPVIDEEGRLRADYKELQPQV
                     QDRVKQLWDQVTSENLYEMTDFVGYKQEFLRLFGFEIPGVDYEADVDPDVKISNLIQL"
                     /name="fabV"
     gene            2573..2956
                     /locus_tag="HV092_RS27975"
                     /old_locus_tag="HV092_27980"
     CDS             2573..2956
                     /locus_tag="HV092_RS27975"
                     /old_locus_tag="HV092_27980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_085452092.1"
                     /translation="MSYVHVIVSMKKSPSKAKEKTKHYMQLDCTKDEALEDILVPYASN
                     SNRILVEGAFISAADIEKIAVFESSDDSKTLYEKESAESEARSARMASQGVIGFFGTTM
                     YAAIHNQATDITSQILREAMKRQ"
                     /name="hypothetical protein"
     Typing          3490..3674
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="83.0"
                     /evalue="1.62e-49"
ORIGIN
        1 acctgacccc cgctgagaaa aagtttatcg acgacgctat agccgctgcc gaacgtgcgg
       61 ccgggaagaa gctcaatcag cctaaccggc acatcgtcct gaacagagcg cgtgcgcaga
      121 ttgagtcgca gcgttacgcc gaccgccagc gtgcattacg tgaggatgag cggcagcagt
      181 ccgattttgc ctggtcacgt ccgcgcgcgc cgcgccgctg attgcggatt ttggcggttt
      241 agtggtgctg acgctgcgaa atcacctgcg attcacccgc gtgcatagcc atgcagtcgc
      301 gcagaaatca tcaggggcgt ttttccggga tgcgggtgag gaaatgtgcc ggaaacggct
      361 ctgatgcgcc agaattgcgc tgagggcctg tcagggggtg ctgataaggg gtcgcttcag
      421 gaaacggaaa ataaagcacg ctaagccgtc gttccaccca cctgccagct ctggtatgac
      481 gttggcggtg tcagtctggg gcatgcccca actggacgtc agaccagaga ggtaaaaccg
      541 tcagagtaga gccgttttcc gaatttgtgg acaccattga tgcgcggtct atggccacaa
      601 cctgacacct tgcgtccctt agcgtacgat tttttccgtt ttctgaagcg acccctgacc
      661 gacgtcagca tccccaccgg tgctcggtgg attcccaagg gcatgagcgt ggaatacctg
      721 gccaaggcca agggcaatgt gcgcgcggtg gccgacggca gcgccgtgga ctgggccagt
      781 gccggcgaca agctggtgga agtgaccgcc ctcaacgagg aagaccaggc ggtgttcacg
      841 gcgcggatca ccatgaacgt gaaactcgcc tgagcagaaa caacaacgcc cctcgggctt
      901 gaggggcgtt ggagtgaaac gctacgacag ggttacagct ggatcaggtt gctgatcttc
      961 acgtccggat cgacgtcggc ttcgtagtcg acacccggaa tctcgaaacc gaacaggcgc
     1021 aggaactctt gcttgtaacc gacgaagtcg gtcatctcgt agaggttctc gctggtgacc
     1081 tggtcccaca gctgcttgac ccggtcctgg acctggggtt gcagctcctt gtagtcggca
     1141 cgcaggcgac cttcctcgtc gatcaccggc tcgctgttgt acaggctgtc cttgtacagg
     1201 ccatagacct gctcgatgca accttcgtgg ctgccctgct ccttcatcac cttgaacagc
     1261 agcgacaggt acagcggcat gatcgggatt gccgagctgg cctgggttac cacggccttg
     1321 agtaccgata cgcgagcatc gccgcccaga tcggccagtt gctcgcgcat gccgatgacc
     1381 ttgtggtcca ggtccttctt ggcttcgccg atggaaccgt tccagtagat atcgtgggtc
     1441 agcttctcgc ccagataagt gaaagcggta gtcttcgcgc cttcggccag gactccggct
     1501 tcgtgcagtg cgtcgatcca catctgccag tcttcgccgc ccatcaccgc cacggtgccg
     1561 tcgatctctt cctgagtcgc cggttgcagg gtggtttcct tgacgatttc cttgtcggtg
     1621 tcgacaccgc gcaaggtcac cgacttgccc aggggcttga gggtcgaggt cagcacttca
     1681 ccggtcttgg ggtgggtgcg acgcggcgcg gccaggctgt agaccaccag gtcgatcttg
     1741 cccaggtcac gcttgatggt ctcgatggtt tcggccttga ccgcatcgga gaaagcgtcg
     1801 ccattgatgc tcttggcgta caggcccttg gcttcggcga acttgtggaa cgccgcggtg
     1861 ttgtaccagc cggcggtgcc cagcttgccg tcgacgctgc cgcgctcgaa gaacaccccg
     1921 agggtgttgg cgccgcaacc gaacgctgcg gtgatccgcg ctgccaggcc gtagccggtg
     1981 gacgcgccga gtaccagtac gttcttcggc cctgcgtcga tggcgccctg ctgggtgacg
     2041 taatcgatct gttgcttgac gttggcttcg caaccaaccg gatgggtcgt cacacagata
     2101 aagccgcgaa cgcggggttt gatgaccata cagaatctcc caatttcaat gtgccaggcg
     2161 ccaataaagg cggcggcagt gtcacttgct aagtcgtaga cggggtcgct tcaggaaacg
     2221 gaaaaaatcg tacgctaagg gacacaaggt gtcaggttgt ggccatagac cgcgcatcaa
     2281 tggtgtccac aaattcggaa aacggctcta ctctgacggt tttacctctc tggtctgacg
     2341 tccagttggg gcatgcccca gactgacacc gccaacgtca taccagagct ggcaggtggg
     2401 tggaacgacg gcttagcgtg ctttattttc cgtttcctga agcgaccccg ataacagatg
     2461 ttatggtaaa tcgggcttgt tttttatagg tttgagccat atctaatgat cacgcgtgat
     2521 ggtctttagt gcatcgccga cgaccctgaa atactaatta acagaggttc ccatgagtta
     2581 cgtgcacgta attgtaagta tgaagaagtc tccatctaaa gcgaaagaaa aaacaaagca
     2641 ctacatgcaa cttgattgca caaaagacga agcgctggaa gatatcttgg ttccttacgc
     2701 aagtaattcc aaccgaattc tagttgaagg tgcttttatc tctgctgctg acattgaaaa
     2761 aatcgcggtt tttgaaagtt cagatgacag taaaaccctt tatgaaaaag agtcggctga
     2821 aagcgaagcc cgatctgcgc gaatggcttc acagggagtg atcggtttct ttggcacaac
     2881 aatgtatgca gctatccata atcaggctac tgacatcact tcccaaatcc tgcgggaggc
     2941 catgaaaagg caataggctg cctaatgccc ccgtggtggg tatataaaat cacggggagc
     3001 gtagcgacct tcgtcaagct ctttccttgt gtgtctcacc ctgcaaaagg tttgattttg
     3061 tgggctcagt gttcgacgtt cgtaatcaca ctgttccgct tgctgctgcc tcagatgctc
     3121 ttttatcagg tgccagttat cgtactgcga gacggggtag agcctaactc tttcgcggtt
     3181 aagttacgta tccaccaatt gcctgtttat cctgccggag tccggggcgt agacctgagc
     3241 gtctgaggtg cagatgtgga tttaatgtcg ttgaatggta ttaagctggt agtgacaagg
     3301 ctagccagcc ctgctaggat ttgatttctg acgtctattc ctggcaggca tctgcgaaga
     3361 attagagtac cgaagccctg tgattctgtc acggggcttt tgtacattta gcgcccgaga
     3421 atcaaaaaca tcccttcaaa acttcaggaa cgcccgccgc agtctcacgg caggagcgta
     3481 gcgaccgagt gagcgaggag gcgtcatttc atcattacct gcattctgca cttacgccct
     3541 gcagccgcat tttccccttg ccacatacac gaagcacctc cctgaattcc catgtccgtg
     3601 ccaacatatt aagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc
     3661 ccgaaacccg ctaaaagcac tgacgcgcct gcggcttgcc caacgcctca cggcaccgga
     3721 ttaaaatccg ctcccgttca ccgtttctga ctgcctttca ccgtcgctta cgcttcatcg
     3781 ttcctactgt gtctggcaga ctgcagggac tattctcata cggagataaa catgacgaga
     3841 ctgacaa
//
