LOCUS       NZ_CP058138             4618 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain RHBSTW-00138 plasmid
            pRHBSTW-00138_8, complete sequence.
ACCESSION   NZ_CP058138
VERSION     NZ_CP058138.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148509
            Assembly: GCF_013624775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00138
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0293412188064x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 53 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 27 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058138.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00138"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1463165"
                     /plasmid="pRHBSTW-00138_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(96..845)
                     /locus_tag="HV092_RS27920"
                     /old_locus_tag="HV092_27925"
     CDS             complement(96..845)
                     /locus_tag="HV092_RS27920"
                     /old_locus_tag="HV092_27925"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /GO_function="GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA]; GO:0005515 - protein binding [Evidence
                     IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="WP_074192387.1"
                     /translation="MSQKPLIFISHITEESELAKMLAAFIKKTYLNMFDVFVSSDGENI
                     SSGDRWLESIDNALGSCVIQVSLCSPYSVTRPWINFEAGASWIRRIPVIPICHSGMTKN
                     KLPIPLSLLQGIDMEQSDAMDRFFVTLSDKLGADIPSVDASSLINDYKDWSLNYTFTSI
                     VKSHINNLCSLYPVFSQAFYGGNQSIPIQVRDQDLAEVGKSLDYLVDQDLIGYSFHGMS
                     IGGGVFKTGSMNLTDKYMRVVFPLINQ"
                     /name="toll/interleukin-1 receptor domain-containing
                     protein"
     gene            complement(827..1756)
                     /locus_tag="HV092_RS27925"
                     /old_locus_tag="HV092_27930"
     CDS             complement(827..1756)
                     /locus_tag="HV092_RS27925"
                     /old_locus_tag="HV092_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_064179607.1"
                     /translation="MSNQALDLFNERLPRKPYFTDELQFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRPGAAIDWSDRNAPAPTLTIMNPENGHAHLLYALKTAVRTAPDGKIK
                     PLKYAAAVERALCQKLDADTGYSGLICKNPNHDHWQLAVWQPELYTLDWLADSLDLNAA
                     NDKKIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLKACYERACAYNLKFATPLD
                     ENEVSGIAKSIANWIYKNFTEHSFNDYVVRTHTSEIQSVRGRKGGRVSKGGGRKSSPTS
                     NENVKPWCKEGISRSTFYERKRKCHKSH"
                     /name="replication initiation protein"
     gene            2038..2277
                     /locus_tag="HV092_RS27930"
                     /old_locus_tag="HV092_27935"
     CDS             2038..2277
                     /locus_tag="HV092_RS27930"
                     /old_locus_tag="HV092_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_064179603.1"
                     /translation="MPVSAMRHKKTVSVTIEPALLHQAREAGINLSATLTTALKHQIRE
                     SEAERWKRANRKAIQELNRITDEHGLLSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            2277..2585
                     /locus_tag="HV092_RS27935"
                     /old_locus_tag="HV092_27940"
     CDS             2277..2585
                     /locus_tag="HV092_RS27935"
                     /old_locus_tag="HV092_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_037427062.1"
                     /translation="MQFMVYRNNSNNRAYPYLLDVQSDIIDELNTRLVIPLFLLDEVNG
                     RPARRLTPVLNVDGEDFLVMTHEMASVRQSQLGEEVVSVREHRQAIKNALDFLLDGF"
                     /name="CcdB family protein"
     gene            complement(2689..2925)
                     /locus_tag="HV092_RS27940"
                     /old_locus_tag="HV092_27945"
     CDS             complement(2689..2925)
                     /locus_tag="HV092_RS27940"
                     /old_locus_tag="HV092_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_037427059.1"
                     /translation="MTETEKLLLSALERLQDDYETRQQAWQASYDSLQTMFTTTSAALM
                     RSDQACQHLSGQVESLRQQVMSLTEAVSRLTRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2986..3471)
                     /locus_tag="HV092_RS27945"
                     /old_locus_tag="HV092_27950"
     CDS             complement(2986..3471)
                     /locus_tag="HV092_RS27945"
                     /old_locus_tag="HV092_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064179604.1"
                     /translation="MSGILDLAKAFEETSKQQAQRTGESVKSEFVKLEQSISEALKSSG
                     KNISDAIHEQNVRQTTLLRRSVTIALIWILFCGVLLIGILSGILWFQGTLIASRLDEMN
                     SYSQQLTDLKAKSGAGVEILTDDTRKNKYYVVLPKSAKGVELYTSKNDKQVVTYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3488..3940)
                     /locus_tag="HV092_RS27950"
                     /old_locus_tag="HV092_27955"
                     /pseudo=""
     CDS             complement(<3488..3940)
                     /locus_tag="HV092_RS27950"
                     /old_locus_tag="HV092_27955"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4140..4475)
                     /gene="mobC"
                     /locus_tag="HV092_RS27955"
                     /old_locus_tag="HV092_27960"
     CDS             complement(4140..4475)
                     /gene="mobC"
                     /locus_tag="HV092_RS27955"
                     /old_locus_tag="HV092_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_074192386.1"
                     /translation="MDKRTKEIKIRLTESEHERLLARCDRAYLAEWLRSLGLGEHVLRR
                     RRVPDVDPVLLRQVTGIGNNLNQIARKLHGLDSLSAHQQVSLLAVLTSFDRQLSELLET
                     QRDRQDS"
                     /name="mobC"
ORIGIN
        1 tagagtatgc cttattctgt ggtggctgca aaaaaaacag ccctctctag cagagggctg
       61 ttatctgata taggcttgtt tcagtccgat gatgcttact ggttaatgag agggaaaact
      121 actctcatat atttgtcggt taaattcata gatccagttt taaatactcc accgccgata
      181 gacattccat ggaagctata accaataaga tcctgatcca caaggtaatc taaagattta
      241 ccaacctccg ctaaatcctg atctctaacc tgaataggta tagactggtt accaccatag
      301 aaagcttgag aaaacactgg atacaatgaa caaagattat ttatgtgtga tttaacgatt
      361 gatgtgaatg tataatttaa cgaccagtct ttgtaatcat taatcaatga agatgcatct
      421 actgaaggaa tgtcagcgcc tagtttgtcc gatagagtaa caaaaaaacg gtccatggca
      481 tcagactgct ccatatcaat cccttgtaac aaggatagag gaattggtaa tttatttttt
      541 gtcattccag agtgacaaat aggaataaca ggaatacgcc taatccaaga agcacctgcc
      601 tcaaaattta tccatggcct ggttacggag taaggactac acaaagaaac ttgtattaca
      661 caagatccaa gcgcgttatc aatgctttca agccatctat cacctgaaga tatattttct
      721 ccatctgaag aaacaaaaac atcaaacata tttaggtatg ttttttttat aaacgcagcc
      781 aacattttgg caagctcaga ctcttcggtt atatgactta taaatattag tggcttttgt
      841 gacatttccg ttttctctca taaaatgttg atcgactgat accttcttta caccacggct
      901 taacattctc atttgatgta ggggatgatt ttcttcctcc ccctttactt acacggcctc
      961 ctttcctgcc cctaactgac tgaatttcag atgtatgagt tctaacaaca taatcattaa
     1021 aactgtgttc agtgaaattt ttataaatcc aattagcaat gcttttggct atcccagaca
     1081 cttcattttc atccaaagga gtagcgaatt tcaggttata ggcacaggct cgttcatagc
     1141 atgctttcag ccactgctca tactctggcc atccctgacg aatagctcgg tatgcccact
     1201 ttcgggtttt atcgaacaag gtgcagttcc ggcctaaacc gtagtcggca acgattttct
     1261 tgtcatttgc agcgttcagg tcgagtgagt ccgccagcca gtccagcgtg tagagttccg
     1321 gctgccacac ggcaagctgc cagtggtcat ggttcggatt cttgcagatt aaccccgaat
     1381 agccggtatc agcgtccagt ttttgacaca gcgcacgctc aaccgctgcc gcatacttca
     1441 gcggcttaat ttttccgtca ggtgccgtgc gcaccgctgt tttcagcgca tacagtaagt
     1501 gggcatgtcc gttttccgga ttcatgatgg tcagcgttgg cgcaggtgcg ttccggtcgc
     1561 tccagtctat cgccgcgccg gggcggtcaa cgtcaaaacc gagccagaac atcgcgtgtg
     1621 gctggttgaa ctggatgtat ttcgcaagaa tggcgcgctc tttcccggca atgcgtactc
     1681 cgaactggag ttcgtcggta aaatagggtt tgcggggaag ccgctcgtta aaaaggtcga
     1741 gtgcctggtt actcaatctc gcctgcgctt atggcgggct gttgtttcgc acggttcatg
     1801 tgctagtatc cttcttaggt tttgtgcctg cgccctcgtt aatcatgtcc ttccgccaag
     1861 tcgagcatga ttacgggggc gtcgtctttt ctgccgctta acgttacaca ccccgccgta
     1921 ttttgtcacc tgtcaccgtc agttattgcc tgaagcaaca gaccattacc ttccgggtaa
     1981 aaaacgcgta cactggcaac acgtgctata tacacgtgta aaaatcagga gatcatcatg
     2041 cccgtatcag cgatgcgcca taaaaaaacc gtcagtgtga caatcgagcc tgcgcttttg
     2101 catcaggccc gcgaagcagg gattaatctg tctgccacgc tcaccaccgc cctgaaacat
     2161 cagatccgcg agtcagaagc ggagcgctgg aaacgcgcca accgcaaggc aatccaggag
     2221 ctcaaccgta ttacggacga gcacggctta ctctctgacg atcacaggac gttctgatgc
     2281 agttcatggt ttaccgcaat aacagcaaca accgggctta tccctatctg ctcgatgtac
     2341 agagcgatat tatcgacgaa ctgaataccc gcctggtgat cccgctgttt ctgctggatg
     2401 aagtgaatgg acggcccgcc cgccgcctga ccccggtgct gaatgtggac ggtgaggatt
     2461 ttctggtcat gacacatgaa atggccagcg ttcgccagtc acagctcggg gaagaggtgg
     2521 tgagcgtccg ggagcaccgg caggccatca agaatgcgct ggattttctg ctcgacggtt
     2581 tctgagttta cctttccatc ccgtgatcgt gtccccggct gcgtccgggg gcaaatgagc
     2641 gcgttttctg tcgttcctgt ttctgcgcca gctccctgtc cagtctggct acctgcgcgt
     2701 caagcggctg acggcttcgg ttaagctcat gacctgctgc cgcaagctct ccacctgccc
     2761 gctcaagtgc tgacacgcct ggtcgctgcg catcagcgcg gccgaagtag tggtgaacat
     2821 ggtctgcaag ctgtcgtaac tggcctgcca tgcctgctgt cgtgtctcgt aatcgtcctg
     2881 taagcgctct aacgcactca gcagcagttt ttcggtctcg gtcatggatt tgcttgtcct
     2941 tatcgctaaa aacgccgtat ggcgcttctg tggcaactga cggggctatt tcgcggtgta
     3001 ggtcacgacc tgtttgtcgt ttttcgaggt gtacagctcg acgcccttcg cgctcttcgg
     3061 cagcaccacg taatacttgt tcttccgggt gtcgtccgtg aggatttcca cccccgcccc
     3121 cgatttcgct ttcaggtcgg taagctgctg gctgtagctg ttcatctcgt ccagacgtga
     3181 ggcgatgagt gtcccctgaa accacagtat ccccgacaga ataccgatca gcagcacacc
     3241 acaaaacaat atccatatca gcgcgatcgt gaccgagcgc cgcagcaggg ttgtctgcct
     3301 gacgttctgt tcgtggatag cgtcgctgat gttcttcccg cttgatttca aggcctcgct
     3361 gatggattgc tcgagcttca caaactcgct cttcacgctc tcccctgtac gctgtgcctg
     3421 ctgcttcgag gtctcttcga aggctttcgc taaatctaaa atcccgctca tataacgccc
     3481 cttttaaccg gatatttcgt ccaccgtccg ggtcggcaat gctgagactg cttttcgttt
     3541 cccgcaccac cgtcagcccg ccgttttcca gcgccctgac cacattctca cggttgcgga
     3601 tcataccctg tgccaccatc gtttccagcg agtccgtaat ggcctgcgcc gccttctgac
     3661 ggtcgccggg aaggtcattg cccggtgtaa gggtgcgtcg tcgggcagga tcatgagggt
     3721 catcaagatt caggcggtca ttggtcagag tctgccaggc gttgaccctg ggacgatcag
     3781 cccggtcata gtagggttgc aggcgttttc cgctctgcaa ttcgatgttc gggaacacaa
     3841 aattcagctc cagcctgtcc ttgtcccggt gctccaccca cagacaggcg tactggtcgc
     3901 cgtccagacc gggcaacagt gtctgctcaa actcatccat cagacgcact ttctcatcgt
     3961 cagggaggtt ttgttcccgg aacgacaaca ccccggaggt ataggcgcgg gcaaactcac
     4021 aaccatcaat cagttgcctg acacgctcag gctcaccgcg cagtaccacc gctttatccc
     4081 gatcacgttc ttttcccagc aggtaatcca ccgggccact gcctgaaccc gcaccgcggc
     4141 tatgaatctt gacgatcacg ctgtgtctcc agaagctcag ataactggcg atcaaagctg
     4201 gtcagtaccg ccagaagaga aacctgctga tgggccgaaa gcgaatccag accatgcagc
     4261 ttacgagcta tctgattaag gttattgccg ataccggtca cctgccgaag cagtaccgga
     4321 tcaacgtcag gcacacgccg acgacgcaat acatgttcac ccagaccgag cgaacgtagc
     4381 cattcagcta gataagcacg atcacaacgt gccagtaatc gctcatgttc actctcggtc
     4441 aggcggattt ttatctcttt cgttcgcttg tccatgacac tccactgaga aaactttcgc
     4501 acccgagtgc gaattttcgc agtggatgcc aggggtttcg gggggcggcg agccccctga
     4561 accagtcaca gaaggaaccc tcgcagaggg gacgctgtgt gtactggctt aaagtatg
//
