LOCUS       NZ_CP058136            12557 bp    DNA     circular CON 03-APR-2024
DEFINITION  Klebsiella quasipneumoniae strain RHBSTW-00138 plasmid
            pRHBSTW-00138_6, complete sequence.
ACCESSION   NZ_CP058136
VERSION     NZ_CP058136.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148509
            Assembly: GCF_013624775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00138
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.0293412188064x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 53 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 27 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058136.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00138"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:1463165"
                     /plasmid="pRHBSTW-00138_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(12327..12557,1..96))
                     /locus_tag="HV092_RS27805"
                     /old_locus_tag="HV092_27810"
     CDS             complement(join(12327..12557,1..96))
                     /locus_tag="HV092_RS27805"
                     /old_locus_tag="HV092_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000415578.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(493..3522)
                     /locus_tag="HV092_RS27810"
                     /old_locus_tag="HV092_27815"
     CDS             complement(493..3522)
                     /locus_tag="HV092_RS27810"
                     /old_locus_tag="HV092_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_012077404.1"
                     /translation="MTTKSERLTVLSDAEQEALYGLPDFDDAQRLEYLALTETELALAS
                     SRPGLHAQVYCILQIGYFKAKHAFFRFDWSEVEHDCAFVLSRYFHGESFEHKPISKHEH
                     YTQREWIADLFGYRPWAAEFLAQLAQQAAQTVRRDVMPGFIAAELIVWLNEHKIIRPGY
                     TTLQELVSEALSAERRRLAGLLSEVLDESAKAALGRLLVRDDTLSQLAALKQDAKDFGW
                     RQMAREREKRATLEPLHRIAKALLPKLGVSQQNLLYYASLANFYTVHDLRNLKADQTYL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFVAEQVRRQQDTPQVGRLLS
                     LYIDDSVPDPTPFGDVRQRAYKIMPRDTLQTTAQRMSVKPVSKLALHWQAVDGLAERIR
                     RHLRPLYVALDLAGTDPGSPWLVALAWAKDVFAKQQRLSQRPLAECPAATLPKRLRPYL
                     LTFDADGKPTDLHADRYEFWLYRQVRKRFQSGELYLDDSLQHRHFSDELVSLDEKAAVL
                     AQIDIPFLRQPLDAQLDALATELRAQWLAFNRELKQGKLTHLEYDKDTQKLTWRKPKGE
                     NQKAREKAFYEQLPFCDVADVFRFVNGQCQFLSALTPLQPRYAKKVADADSLMAVIIAQ
                     AMNHGNQVMARTSDIPYHVLESAYQQYLRHATLHAANDCISNAIAALPIFPYYSFDLDA
                     LYGAVDGQKFGVERPTVKARHSRKYFGRGKGVVAYTLLCNHVPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIVPTAITGDMHSVNKANFAILHWFGLRFEPRFTDLGDQLKELYSADDPA
                     LYDQCLIRPAGRIDRDLIVSEKPNLDQIVATLGLKEMTQGTLIRKLCTYTAPNPTRRAV
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRIESYHQLRSTIAQVGGKKELTGRTDIEI
                     EISNQCARLIANAVIFYNSAILSRLLMKYEASGNAKAHALLTQISPAAWRHILLNGHYT
                     FQSDGKMIDLDALVAGLELG"
                     /name="Tn3 family transposase"
     gene            complement(3519..4112)
                     /locus_tag="HV092_RS27815"
                     /old_locus_tag="HV092_27820"
     CDS             complement(3519..4112)
                     /locus_tag="HV092_RS27815"
                     /old_locus_tag="HV092_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435588.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_012435588.1"
                     /translation="MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKDD
                     RAGLARALEFVRPGDVLVVWKLDRLGRSLSHLLAIVTSLKKKQVAFRSLTENLDTTTPS
                     GEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALDGGM
                     SKAAVCRNFGVKRTTLIETLARVGWTGSRGASSR"
                     /name="recombinase family protein"
     gene            4351..4629
                     /locus_tag="HV092_RS27820"
                     /old_locus_tag="HV092_27825"
     CDS             4351..4629
                     /locus_tag="HV092_RS27820"
                     /old_locus_tag="HV092_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011342941.1"
                     /translation="MSKQAVFTMKLEPELRAEFMAEAEAAHRPASQVLRELMREFVQRQ
                     RESREYDEFLRRKVEAGRASMRAGLGRSNDEVEAEFAARRASVASQA"
                     /name="hypothetical protein"
     gene            4626..4910
                     /locus_tag="HV092_RS27825"
                     /old_locus_tag="HV092_27830"
     CDS             4626..4910
                     /locus_tag="HV092_RS27825"
                     /old_locus_tag="HV092_27830"
                     /function="post-segregational-killing"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="WP_011342942.1"
                     /translation="MRVVWTPEAQQDRADVWDYIAADNPRAAARMDEIFSDAAARLIQH
                     PMLGKPGKIPGTRELIPHESYRLVYQIDGETVWILTLVHTARLWPPVRD"
                     /name="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
     gene            5317..5877
                     /locus_tag="HV092_RS27830"
                     /old_locus_tag="HV092_27835"
     CDS             5317..5877
                     /locus_tag="HV092_RS27830"
                     /old_locus_tag="HV092_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514928.1"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA];
                     GO:0016491 - oxidoreductase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="WP_011514928.1"
                     /translation="MDTRLFAFVGADIGPWRIVRAETRVGEPLPEAKRLNVVSASELQS
                     ETNAPWILRGITSNERYVMRAEKNEIVAKQQGLARPEATCGALIPIRKNAAWWELTQDE
                     RRSVFEQSKHVQIGLQYLPAVARKLHHCRDLSENEPFDFLNWFEYAPIHEVEFNRLLSE
                     LRASEEWKYVDREVDIRLTQAQV"
                     /name="chlorite dismutase family protein"
     gene            6011..6367
                     /locus_tag="HV092_RS27835"
                     /old_locus_tag="HV092_27840"
     CDS             6011..6367
                     /locus_tag="HV092_RS27835"
                     /old_locus_tag="HV092_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_012435589.1"
                     /translation="MEMESRIFSVTEYIRPSDGEPIRSVVLETKDSAVVVWHAHPGQEI
                     TAHVHPDGQDTWTVISGEAEYYQGGGKVAHLKAGDIAIAKPGQVHGALNTSPVPFVFVS
                     VVASGNAGFALAEK"
                     /name="cupin domain-containing protein"
     gene            complement(6572..6934)
                     /locus_tag="HV092_RS27840"
                     /old_locus_tag="HV092_27845"
     CDS             complement(6572..6934)
                     /locus_tag="HV092_RS27840"
                     /old_locus_tag="HV092_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009629415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_013722854.1"
                     /translation="MSTHRVYKSHEFMQPAEKEPIRSVVVESSHSVIVAWHVEPGQTIA
                     PHIHPDGQDTWTILSGEGQYQIDEQGNTVAIVPGDIVVANKGQVHGVLCTSVGPLRFIS
                     VVAPLDAGYEPLSAKT"
                     /name="cupin domain-containing protein"
     gene            complement(7090..7623)
                     /gene="aadB"
                     /locus_tag="HV092_RS27845"
                     /old_locus_tag="HV092_27850"
     CDS             complement(7090..7623)
                     /gene="aadB"
                     /locus_tag="HV092_RS27845"
                     /old_locus_tag="HV092_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_032490446.1"
                     /translation="MDTTQVALILQFLAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(7815..8312)
                     /locus_tag="HV092_RS27850"
                     /old_locus_tag="HV092_27855"
     CDS             complement(7815..8312)
                     /locus_tag="HV092_RS27850"
                     /old_locus_tag="HV092_27855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181352637.1"
                     /translation="MESKDEIIQVIKNNVPSYQTDKLVKLYMSDADESDYYNLAKKFLG
                     ESTDNGSWIATGTSSFGVEKKSFFTCLKNEVYLLFCSDDEKYSEYRKKIDSNIDKAVGA
                     AVVAIGVLSRNRRKFRQPINVACPAPGRWGRPNSRVTSRHFDHGNASRTSRPDCSGLDE
                     AD"
                     /name="hypothetical protein"
     gene            complement(8314..8985)
                     /locus_tag="HV092_RS27855"
                     /old_locus_tag="HV092_27860"
     CDS             complement(8314..8985)
                     /locus_tag="HV092_RS27855"
                     /old_locus_tag="HV092_27860"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]; GO:0008236 - serine-type peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="WP_118405127.1"
                     /translation="MFKNLVERYNGACFALAKKNGGNSISFIGSCFLSHESGLFITCSH
                     LVSPLDELCVLAPMDANSFTPITIKGSVNIIDVKLCAMNTDADLAILKSTIDIKAQVPA
                     GMLLTDSPNTGASCMYIGYPYGDVGLHNPKYSASILSGKVIIDDINYYLIDSLAHEGNS
                     GGPLIDYQTGRICGVINGHFNPTGGGAVIQLGNRTLGTDSTITKAVSIEYAIVLIAEVL
                     S"
                     /name="serine protease"
     gene            complement(8970..9896)
                     /locus_tag="HV092_RS27860"
                     /old_locus_tag="HV092_27865"
     CDS             complement(8970..9896)
                     /locus_tag="HV092_RS27860"
                     /old_locus_tag="HV092_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_118405126.1"
                     /translation="MHNAALELFNDRLPHKPYFSDDLNFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLSITNPDNGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGYWKIAVWQPELYTLDWLADSLDLSAA
                     NDKDIAADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERACAYNLQFALPLD
                     ENEVMGIAKSISKWTSNHFSGDSFEQYVVATHSSEIQSIRGRIGGKKSIGGGRPKDANS
                     VNQIKPWVKENISRRTYFYRKAKCLKI"
                     /name="replication initiation protein"
     gene            complement(10090..10374)
                     /gene="nadS"
                     /locus_tag="HV092_RS27865"
                     /old_locus_tag="HV092_27870"
     CDS             complement(10090..10374)
                     /gene="nadS"
                     /locus_tag="HV092_RS27865"
                     /old_locus_tag="HV092_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(10376..10708)
                     /locus_tag="HV092_RS27870"
                     /old_locus_tag="HV092_27875"
     CDS             complement(10376..10708)
                     /locus_tag="HV092_RS27870"
                     /old_locus_tag="HV092_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094966193.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10870..11103)
                     /locus_tag="HV092_RS27875"
                     /old_locus_tag="HV092_27880"
     CDS             complement(10870..11103)
                     /locus_tag="HV092_RS27875"
                     /old_locus_tag="HV092_27880"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_016946053.1"
                     /translation="MTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALT
                     HSDRVCQHLSSQVESLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(11164..11649)
                     /locus_tag="HV092_RS28350"
     CDS             complement(11164..11649)
                     /locus_tag="HV092_RS28350"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139110915.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMIRTSVSAAMAWVFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLANLKAKSGAGVTIYSDDTRRNTYLAVLPKQAHGVETYTSTTGNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<11696..12118)
                     /locus_tag="HV092_RS28355"
                     /pseudo=""
     CDS             complement(<11696..12118)
                     /locus_tag="HV092_RS28355"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             complement(7094..7623)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(10773..12337)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccactccgca aggtgagtcc ggtcgcagcg atcaagcagt cgctgatgct cggtttcggt
       61 gagccggatt ttgatctctt tcgttcgctt ttccataggc tccgctgaga aaaaattcgc
      121 accaccgtga gaatttttcg cagtggatgc aggggtttcg gggggcggcg agccccctga
      181 accagtcacg gtgacacctc gttagagggg acgctgtgtg tactggctta gagtgtattg
      241 gagtatgtca gattctgcgc tatctgcaaa aaaaaagccc tctcataaag agggctgtta
      301 tctgatatag gcttgttttg gtgcaatcat ccttcagatg aagctactgg tttatttgct
      361 tcacaccatg cattcttggt taacttatat atgcataaaa ctaaacaagt tacagcccca
      421 gctattaacc cagtggatat attcagtgtt gtagctatag ggggttctaa gccagaaccg
      481 ccgaaatttc cgtcatccca gctccagccc cgccacgagc gcatccaggt cgatcatctt
      541 gccgtcgctc tggaaggtgt aatgcccgtt cagcaggatg tgccgccagg ccgccggcga
      601 tatctgggtc aggagagcgt gcgccttggc gttgccgctc gcctcgtact tcatcagcag
      661 ccgcgagagg atggccgagt tgtagaagat gaccgcgttg gcgatcagcc tggcgcactg
      721 gttgctgatc tcaatttcga tgtcggtgcg cccggtcaat tccttcttgc cgccgacctg
      781 ggcgatggtt gagcgtagct ggtgatagga ctcgatgcgg ttctgtgagc ggtgaacgtt
      841 gcgctccagt tgcggatcgc gcaggtagcg cagcgtgtag atgctgcgga tgagcttgtc
      901 gaactcgaac accgcgcgcc gcgtggggtt cggcgcggtg taggtgcata gcttgcggat
      961 cagcgtgccc tgcgtcatct ccttcagtcc gagcgtggcg acaatctggt cgaggttcgg
     1021 cttctcgctg actatgagat cgcggtcgat tctcccggcc ggccggatca ggcactgatc
     1081 gtacagcgcc ggatcgtcgg cactgtagag ttccttcaac tgatcgccaa ggtcggtgaa
     1141 gcgcggctcg aaacgcaggc cgaaccagtg caggatagcg aagttggcct tgttgacgct
     1201 gtgcatgtcg ccggtgatcg cggtcggcac gatgtccgac gtgttgcgat accagatgtc
     1261 gaacacgtga tgggcctcgt aatcgtgcgc gccgatcagg tagccgttga gcggcacgtg
     1321 gttgcacagc agcgtgtagg cgaccacgcc cttgccgcgc ccaaagtatt tgcgcgagtg
     1381 gcgcgctttc acggtcggcc gctcgacgcc gaatttctga ccatcgacgg caccgtacag
     1441 tgcatcgagg tcgaacgagt agtacgggaa gatcggcagc gcggcgatgg cgttgctgat
     1501 gcagtcgttg gccgcgtgca gcgttgcgtg gcgcaggtac tgttggtagg cgctctccag
     1561 cacgtggtac gggatgtcgc tggtgcgtgc catgacctgg ttgccgtggt tcatcgcctg
     1621 cgcgatgatg accgccatca ggctgtcggc gtcggcgacc ttcttcgcat agcgcggctg
     1681 caaaggcgtc agcgccgaca ggaactggca ctggccgttg acgaagcgga acacgtcggc
     1741 cacgtcgcag aacggcagtt gctcgtagaa cgccttctcg cgcgccttct ggttctcgcc
     1801 cttgggcttg cgccatgtca gcttctgcgt gtccttgtcg tattctaggt gcgtcagctt
     1861 gccctgcttc agctcgcggt tgaaggccag ccactgagcg cgcagctcgg tcgcgagcgc
     1921 atcgagctgg gcatcgagtg gctgccgcag gaacgggatg tcgatctgcg ccagcacggc
     1981 ggccttctca tccagcgaaa ccagctcgtc ggaaaaatgc cggtgctgca agctgtcgtc
     2041 gaggtagagt tcacccgact ggaagcgctt cctgacctgg cggtacagcc agaactcgta
     2101 gcggtcggca tgcaggtccg tcggcttgcc atcggcatcg aaggtcagca ggtacggtcg
     2161 caagcgtttc ggcagcgtgg ccgctggaca ttcggcgagc ggccgttgcg ataggcgctg
     2221 ctgtttggcg aacacgtcct tggcccaggc cagcgccacg agccacgggc tgcccggatc
     2281 agtgccagcg aggtcgagcg cgacatacag cggccgaaga tggcggcgga tgcgctcagc
     2341 caggccgtcc accgcctgcc agtgcaaagc cagcttgctc accggcttca cgctcatgcg
     2401 ctgcgcggtg gtttgcagcg tatcgcgggg catgattttg taggcgcgct ggcgcacatc
     2461 gccgaacggc gtgggatcgg gcacgctgtc gtcgatgtaa agcgacagca ggcggccgac
     2521 ctgcggtgtg tcttgctgac ggcgcacctg ctcggcgaca aaggattgct ttgcgcccgc
     2581 actgctttcg tcctccaact gcttcatgtg gtaggccatc gcatcgacca ggttgtcgga
     2641 aagctgccgg tagcgcaccc aggcatagca aagcaggtag aggtaggtct gatcggcctt
     2701 caggttgcgt agatcgtgga cggtgtagaa gttcgccagg ctggcgtagt acagcagatt
     2761 ctgctgcgag acgccgagct tgggcagcag cgccttggcg atccggtgca gcggctccag
     2821 cgtggcgcgc ttttcgcgtt cgcgggccat ctgacgccag ccaaagtcct tggcgtcctg
     2881 cttgagcgcc gccaattgcg acagggtgtc gtcacgcact agaagccgac ccagcgcggc
     2941 cttggccgat tcgtccaaca cttccgacag caggccagcc agccgccgac gctcggcgga
     3001 cagggcttcg ctcaccagct cttgcagggt ggtatagccg ggccggatga tcttgtgctc
     3061 gtttagccag acgatcagct cggcggcgat gaaccccggc atcacgtcgc gccgcacggt
     3121 ctgcgcggcc tgctgcgcga gctgcgccag gaactcggcc gcccacggcc ggtagccgaa
     3181 cagatcggca atccactcgc gctgggtgta gtgctcgtgc ttggagattg gcttgtgctc
     3241 gaaggactcg ccgtggaagt agcggctcag cacgaaggcg caatcgtgct cgacctcact
     3301 ccagtcgaag cggaagaagg catgcttggc cttgaagtaa ccgatctgca agatgcaata
     3361 gacctgggca tggagaccag gccggctgct ggcgagcgcc agttcggttt cagtcaacgc
     3421 caagtattcc agccgctggg cgtcgtcgaa gtccggcagg ccgtacaggg cttcctgctc
     3481 ggcgtccgac aggacggtca atcgttcgct cttggtcgtc atcgcgatga cgctccacga
     3541 gagcccgtcc aaccaacccg tgccagggtc tcgatcagcg tggttcgctt gacgccgaag
     3601 ttgcggcaca ccgccgcctt ggacatgccg ccatcgagcg cagcgacgat ggcctccagc
     3661 ttctcgccgg tgatcgcctg cggccggccg ccgatccggc cgcgtttgcg ggcggcagcc
     3721 agaccggcga cgacacgttc ctggatcaag gcgcgttcgt actgcgcgag cgcgccgaac
     3781 acctggaaca gaaactcgcc cgagggcgtc gtggtatcca ggttctccgt cagcgagcgg
     3841 aacgccacct gctttttctt gagcgaggtc acgatggcga gcaagtgcga caacgaacgg
     3901 ccgagccggt cgagcttcca cacgaccaac acgtcgccag ggcgaacgaa ttcgagcgcc
     3961 cgcgccaggc ccgcgcggtc gtccttcgcg ccggaagcat gatcctcgaa cagatgccgc
     4021 gcatcgacgc cgacggcgag cagcgcatcg cgctgcaagt tcgtgctctg gcggtcggag
     4081 tccgacgaca cgcgcatgta acctaccaac ataagcggaa aaccattaag acgaggtttc
     4141 cgtatggtag cgtctggcga cagagttttc cgcacaattt tgcggccact cggaggttgg
     4201 tgcagaggac cgttgaaggc ctgtcgaaaa acaaatgttt tccgacaggc cttggcctag
     4261 cgcacgcccg ccttgcagcg ttctgtgaat agcgcttagt aatgacggcg tatatacttt
     4321 gttagtatca agtggcagga ggccccgatc atgtcaaaac aagccgtttt cacgatgaag
     4381 ctggagcctg agttgcgcgc cgagtttatg gccgaagccg aggcggccca tcgcccggcg
     4441 tcgcaagtgc tgcgcgagct gatgcgcgag ttcgttcagc gccagcgcga gtcgcgcgag
     4501 tacgacgagt tcctgcgccg caaggtcgaa gccggccggg cttcgatgcg cgctggattg
     4561 gggcggtcga acgatgaagt tgaggccgaa ttcgccgcac ggcgtgccag tgtggcgagc
     4621 caggcgtgag ggttgtttgg acgcccgaag cgcagcaaga ccgtgccgat gtgtgggact
     4681 acatcgcagc cgacaatccg cgcgcggcgg cccggatgga tgagattttc agcgacgcgg
     4741 ccgcccgctt gatccagcac cccatgctgg gcaagccggg aaagattccc gggacccgcg
     4801 agttgatccc gcacgaaagc tatcgcctgg tgtatcagat cgacggcgaa acggtgtgga
     4861 tactgacgct ggttcatact gcccgcctgt ggccgcctgt gcgcgattaa gatatgatca
     4921 tggcaacaat gaaacggcgc gttgacagac tgctttcgag ccagaccgcg gtcggcggca
     4981 ggcggccagt tgcggacggt caagcatctt cccggtagcg gacgttcaat agtgtctctg
     5041 acctgcttcg ctccctctat gaagggaggt cgctggccca tccgcgcact cacagcctgc
     5101 cagtaggcgc aaattggcag cgctccgtcc ggcagcgcac agactgcggc cgtacgtccg
     5161 cgtaggttcg cttcagcagt ttcgattcga aagttcaatg tgattgctgc gggcggtcgt
     5221 cgcagttgcg cggaggcgat gatgcacaag cacgggatcg gcagataggt tcttcgaagc
     5281 tgcaagagtt aaaacattca cacatgaaag attgaaatgg atacacgatt atttgctttc
     5341 gtcggcgcag acattggccc ttggcggatt gtcagggccg aaacgagagt tggcgagccc
     5401 ctgcctgaag ccaaaaggct caacgtcgta tccgcttcag agttgcagtc tgaaaccaat
     5461 gcaccctgga tacttcgtgg aataaccagc aatgaacgat atgtcatgcg cgcagagaag
     5521 aatgaaattg tagcgaagca gcaaggtctg gcgcgcccag aagcaacgtg cggtgcgcta
     5581 ataccgatcc ggaagaacgc agcttggtgg gagctcacgc aagacgaacg ccggagcgtt
     5641 ttcgaacagt cgaagcacgt ccaaatcggg cttcagtatt tgcctgcagt agcgcgcaag
     5701 ctccaccact gccgcgacct ctcagagaat gaaccgttcg attttctgaa ttggttcgaa
     5761 tacgcgccaa ttcatgaggt tgagttcaac aggctgcttt ccgaactgcg tgcgtcagag
     5821 gaatggaaat acgtcgaccg agaagtcgat attcgtctta cgcaagcaca ggtctaaccc
     5881 gccgatgaac acggaccccc aacagcggcg cgttgcgccg ccgtttcggg ccggttatcg
     5941 gcaacgtaga actctcggat acagttgttc tgtaataact tctcaagcca aattgatttt
     6001 tggagtggat atggaaatgg aatcaagaat attttcggta acagaatata ttcgtccgtc
     6061 tgacggcgaa ccaattcgtt cagtggttct ggaaaccaag gactcagcag ttgttgtttg
     6121 gcatgcccat cctgggcaag aaataaccgc tcatgttcac ccggacggcc aagatacttg
     6181 gacggttatc tctggagagg ctgagtatta ccaaggaggc ggcaaagtcg ctcatctaaa
     6241 ggctggagat attgccatag caaaacctgg ccaagtgcat ggcgctctaa atactagtcc
     6301 agtgccgttt gtatttgtct cagtggttgc atctggcaat gcgggttttg cgttggctga
     6361 aaaatagtct ttctcaaatg tgtgccccag agagttctaa cccatccatc aacacggacg
     6421 ctgcgcgatg aagccgcgcc gcgccggcta acgcccgcgg taagctgcgc cggagcgaag
     6481 cggagggtac caacaggcgg agcctgttgg cgtcagcttg accaaacagt taggctgggg
     6541 agcggaggca gcttgttgca cttgatatcc cctatgtttt tgctgatagc ggttcataac
     6601 cagcatccaa aggcgcaacc actgaaatga accgaagtgg gccaacgctg gtgcaaagaa
     6661 caccgtgcac ctgccctttg tttgcaacta cgatatcccc cggaacaatt gcaacggtgt
     6721 tgccttgctc gtcaatttga tattgacctt cccctgaaag gatggtccat gtatcttgtc
     6781 cgtcggggtg aatatgtggc gcaatagttt ggccgggctc cacatgccaa gccacgataa
     6841 ctgaatgcga agattcaaca acaacagacc gtattggttc tttctcggct ggctgcatga
     6901 attcatggga cttatacacg cggtgagtgc tcattttttg ctccgtaaat gcaagccttt
     6961 gggcagcaag attcgcgccg cccaaaagtc taacacctga gttaagccgg agcgctttgc
     7021 ggccgcggcg ttgtgacaat ttaccgaaca actccgcggc cgcgaagcga tctcggcttg
     7081 aacgaattgt taggccgcat atcgcgacct gaaagcggca cgcaagacct caaccttttc
     7141 cgccccgagt gaggtgcatg cgagcctgta ggactctatg tgctttgtag gccagtcgac
     7201 tggtggtact tcatcggcat agtaaaagta atcccagatg atcgcctccc agctgttaca
     7261 acggactggc cgcccggcga tgacaccctc agccgcctct gggcacgagc cctgcggagc
     7321 ctccgcgatt tcatacgctt cgtctgccca ccaagcaggt tcgcagtcaa gtaactcatc
     7381 cccgatctcc gctaagaatc catagtccaa ctcctccgtg acgcgcccgc cgagcatttc
     7441 aactattgcc tcgagctcgc cgcgcctctc gccgggaaac gtcagatcaa tatcatcgtg
     7501 cttgcgtgtt acacgcccta gccgtgcatc gatcgcccag cccccaccga tccagagcgg
     7561 cagatttcgc tcatctgccg cagctagaaa ttggagtatc aatgcgacct gcgttgtgtc
     7621 catgcggcct aactccacgt tgagcgtcag ctttccatat gtctgggggt ctgcaacggg
     7681 tcgggtgccg tcgcgtgcgc agtgtcaaag ctgacatgac taatacatga gatgggctga
     7741 tgctcgccgt ccggtcaggc agctgccccc ctgcaggact cacagcagtt gtatgccacc
     7801 cgtcatccga gacgtcagtc ggcctcatcc agaccggaac aatctggacg tgacgtccgg
     7861 ctggcattgc cgtgatcgaa atggcggcta gtgacacgac tgttgggtct accccatcga
     7921 ccgggcgcgg gacaagccac gttgatcggc tgacggaatt ttcggcggtt ccggcttaga
     7981 acccctatag ctacaacagc agcacctact gccttatcta tattagagtc tatttttttt
     8041 ctgtattcag aatatttttc atcatcagag cagaataaaa gatacacctc atttttcaaa
     8101 caagtaaaaa aactcttttt ttcaaccccg aacgatgatg tacctgtagc gatccagcta
     8161 ccattatccg tactttcacc taagaatttt ttagctaagt tataataatc actttcatct
     8221 gcatcagaca tgtataattt aactaactta tcagtctggt acgaagggac attatttttt
     8281 atcacctgga tgatttcatc tttactttcc atgttaagat aatacctcag caataagaac
     8341 gattgcatac tcaatagaaa cagctttcgt tatagtacta tcagtgccta aagttctgtt
     8401 gccaagctga atgacagcgc cgcctccggt ggggttgaaa tggccattga taacaccaca
     8461 aatgcgacct gtttgataat ctatcaacgg gccaccactg tttccttcat gagctaagga
     8521 gtcaattaag tagtaattaa tatcatctat aattactttt ccactcaaaa tagatgccga
     8581 atattttggg ttatggagtc caacatcacc atacgggtag cctatgtaca tacaggatgc
     8641 cccagtgttt ggactatctg ttagcaacat tccagcagga acttgagctt taatgtcaat
     8701 ggtagattta agtattgcaa gatccgcatc agtattcatt gcgcataatt taacatcaat
     8761 tatattgact gagcctttaa tagttattgg tgtaaatgaa ttagcatcca tcggtgccag
     8821 aacacacaat tcatctagag gggataccaa atgagagcag gtgataaaaa gtccactttc
     8881 atgtgataaa aagcagctac ctataaaaga tatactgttc cccccattct ttttagctag
     8941 cgcaaagcaa gctccattat atctttcaac tagattttta aacattttgc tttcctataa
     9001 aaataagttc ttctgctaat attttctttt acccatggtt taatttgatt tactgaatta
     9061 gcatccttcg gcctgcctcc tccaatactt ttcttcccac caatccgacc tcttattgac
     9121 tgaatctcag atgagtgagt tgccactaca tattgctcaa acgaatcacc agaaaaatga
     9181 ttactcgtcc atttagatat gcttttagca atacccatta cctcattttc atcaagaggt
     9241 aatgcaaatt gtagattgta agcacaagct cgttcatagc aggcttgcag ccactgatca
     9301 tactcaggcc agccctgacg gatggcgcgg tatgcccatt tgcgggtttt gtcgaacagg
     9361 gtgcagttac gacctaaacc gtagtcggca gcgatgtctt tgtcgttagc agcactcagg
     9421 tcaagtgaat cagccagcca gtccagcgta tagagttctg gctgccacac agcaattttc
     9481 cagtatccgt ggttcggatt tttgcagatt agtcccgaat accccgcgtc agcctctaat
     9541 tttttacgta gcgcgttctc cacggcggcg gcatagcgca gtggcttcat ttttccgtcc
     9601 ggcgcagtac gaattgaagt tttcagcgcg tacaacagat gcgcgtgtcc attgtcggga
     9661 ttggtgatgc tcagcgtagg agcgggggcg ttgcgatcgc tccagtcgat ggcagcaccg
     9721 gcgcgatcga cgtcgaatcc cagccagaac atggcatgag gctgattgaa ctggatatat
     9781 ttcgccagaa tggcgcgttc cttaccggca atgcgtacac caaagttcag atcgtccgaa
     9841 aagtacggct tgtgcggtaa gcggtcgtta aagagttcga gtgcggcgtt atgcaatgcg
     9901 ccctacgctt atggcgggct gttgtctggc acggttcatg tgctaatatc ctttcaaggt
     9961 ttcgtacctg gccctggtta tcatgtgcat ccgccaagat tgagcatgat ttccggggct
    10021 ttgttttttc tgttgccgct aaattacacg cgaacggcct cgcctgtcga atccttctgt
    10081 cacaacggct tagtgcgccg ccagctcctg gaaaaatttc gggttcgcct ggatggttgc
    10141 cagcaccttt gctgccagcc cggtcgggtt acggcgtccg gtttcccagc tcttgatggt
    10201 atcgagactg gttcccagca ctttcgccat ctcgctctgt gaaacgttca gctgtgccct
    10261 gatggccttc acgtctgcca gctcgtaacg ggtgacgcgg gcaggctctc tgacgccgtt
    10321 tttgatttca acggcttctt ccagcgatgc tttcagctcg tcaaaaaaac tcatgtcaca
    10381 cctcgtcttt cagttgttgg gtcagcgtct tcagcgcggc tttctccgcg tccgtcaggc
    10441 tgtctttggt gcttttcggg taggccatca ccagataaat cacctcggcg gtggccagga
    10501 agtaaatcac cctcgcgctg ccgctttttc cctggtttcc cgtcgccatt ctgatttttc
    10561 gcagcccgcc ggttttctgg atgagatcgc ctttgtccgg cgactcaatc agcgtttttt
    10621 gcagctccct gagctcttca tccgtggcga tctgttttat ctgacgggta aacatcgggg
    10681 tctcgataaa ctcaatcgcg tgactcacgg ctgtctcctg tttttaacgg agtacatagt
    10741 acacttaatt ctgatttcgg aaaagcggtc acatcgaggg gccatagtaa cgagtcgttt
    10801 ttttctgcgg ctccggctct ggctgctgct cacgcagctt ctcggcgtag gcgcggtcgt
    10861 gctgcctgat caacgcatca agcggctgac gttcctgctc aagctctcga cctgcgcgcg
    10921 caagctctct acctgactgc tcaagtgctg acacacccgg tcgctgtgcg tcagcgcctg
    10981 tgaagtagcc tcgaacatgt gctgcaagct gctgtagctg tcctgccacg cctgctgctg
    11041 ttgctcgtag cttgtctgta agctctctaa ggcgctcaga agctgttttt ccatctcggt
    11101 catggttttg gtcgtcctca gtgttaaaaa cgcgctgtgg cgtttcaggg cgctctggcg
    11161 gggttatttt gccgtgtatt tcaccacggt gttgccggtc gtcgacgtgt aggtttccac
    11221 gccatgcgcc tgcttcggca gcaccgccag gtaggtgttc cggcgcgtgt cgtcgctgta
    11281 aatcgtcacg cccgcgccgc ttttcgcttt caggttcgcc agctgctggc tgtagctgtc
    11341 catttcgctc aggcgcgagg tgatgatcgt gccctgaaac cagattatcc cccccatgat
    11401 gccgatcagc agggcgaagc agaggaatac ccacgccatc gccgctgaaa cggaggttct
    11461 gatcatgccc gtcagcctgc cgttctgctc gcggatagcg ttgctgatgc tctgcgagct
    11521 tgattgcagt tccgcgctga tagacgtctc cagcctcgcg aactcgcgct tcacgctctg
    11581 ctcggtatcc tgcgcctgct gcttcgattt ctcctcgaag cccttcgcca aatttaaaat
    11641 ctcgctcata aatcattcct ttcagccgga tatttcggcc gccgtccgga tcggcaatgc
    11701 tgatgctgct tttcgtttcc cgcgctacgg tcaggccgta gctttccagc tgcgtcacca
    11761 cgtcctgccg gctgcggatc acgccctgct gcatcaggtt catcaggccg gcggtgattt
    11821 tctctgccgc ctgctgtttg tcgcggggca gatcgctggc ctgcgtcagc ggacggcggt
    11881 aggtcgggtc attcgggtcg cgcagcccca gccggtcgtt ggtcagggtc tgccaggcgt
    11941 tgacgcgggg acggtcggcg cggtcaaagt agggttgcag gcgttttcca ctctgcaatt
    12001 cgatgttcgg gatgacaaaa ttcagctcaa gccgtccctt gtcgcgatgc tctacccaca
    12061 ggcaggcgta ctggtcgcgg tcgagtccgg tcagcagcgt gtgctcccat tcgtccatca
    12121 ggcgcgattt ctcggcgtct gcaatgtcag gttcctggaa tgaaagcacg ccggaagtgt
    12181 aggcgcgggc aaagttgcag ccgtcgatca gctcacgcac atgttcagga ttgccgcgca
    12241 gtacgctggc ctgttcgcgc tgccggtctt ttcccagcag gtaatccaca ggaccgctgc
    12301 ccgaacccgc accccgcgag tgaattttaa cgatcacggt tctgctccag caggtcgcca
    12361 agctgccgat caaggctggt cagtacgacg agcaacgagg cacgttcaga aggcgtcagg
    12421 gaatcagtct ggttcagacg gcgggcaatc tggttcaggt tgttgccaat cccgctgacc
    12481 tgtcgaagca gttcgggtgc cacgtcaggc acacgacgct tgcgtgaggt gtgttcaccc
    12541 agacctagct gacgcag
//
