LOCUS       NZ_CP058122             6761 bp    DNA     circular CON 09-APR-2024
DEFINITION  Klebsiella michiganensis strain RHBSTW-00167 plasmid
            pRHBSTW-00167_5, complete sequence.
ACCESSION   NZ_CP058122
VERSION     NZ_CP058122.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148522
            Assembly: GCF_013624375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00167
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69.9496584429644x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624375.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:24:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,406
            CDSs (total)                      :: 6,284
            Genes (coding)                    :: 6,107
            CDSs (with protein)               :: 6,107
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 57 of 177
            Pseudo Genes (incomplete)         :: 135 of 177
            Pseudo Genes (internal stop)      :: 31 of 177
            Pseudo Genes (multiple problems)  :: 42 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058122.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00167"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:1134687"
                     /plasmid="pRHBSTW-00167_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6626..6761,1..26))
                     /locus_tag="HV105_RS31855"
     CDS             complement(join(6626..6761,1..26))
                     /locus_tag="HV105_RS31855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            431..622
                     /locus_tag="HV105_RS31590"
                     /old_locus_tag="HV105_31590"
     CDS             431..622
                     /locus_tag="HV105_RS31590"
                     /old_locus_tag="HV105_31590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_181250936.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKHHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            622..1044
                     /locus_tag="HV105_RS31595"
                     /old_locus_tag="HV105_31595"
     CDS             622..1044
                     /locus_tag="HV105_RS31595"
                     /old_locus_tag="HV105_31595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1792..>2040)
                     /locus_tag="HV105_RS31600"
                     /old_locus_tag="HV105_31600"
                     /pseudo=""
     CDS             complement(1792..>2040)
                     /locus_tag="HV105_RS31600"
                     /old_locus_tag="HV105_31600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <2039..2521
                     /locus_tag="HV105_RS31605"
                     /old_locus_tag="HV105_31605"
                     /pseudo=""
     CDS             <2039..2521
                     /locus_tag="HV105_RS31605"
                     /old_locus_tag="HV105_31605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            2523..2780
                     /locus_tag="HV105_RS31610"
                     /old_locus_tag="HV105_31610"
     CDS             2523..2780
                     /locus_tag="HV105_RS31610"
                     /old_locus_tag="HV105_31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(2931..4205)
                     /locus_tag="HV105_RS31615"
                     /old_locus_tag="HV105_31615"
     CDS             complement(2931..4205)
                     /locus_tag="HV105_RS31615"
                     /old_locus_tag="HV105_31615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222061.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu2 family transposase"
                     /protein_id="WP_011191341.1"
                     /translation="MTIINATQYLKQLLSSSELNRIGKFTGFCQRLRDIQPARLLPALL
                     SGLGCDKVDGIAGLHRHFNALQLHDTDQIAYKPFHNQLRKQGFPLFMRALVERAIALRL
                     KECLPDAHGLAGFEQVLLQDGSSFALHPQLAEHFPGRFNKHSPAAVECHMTMSLLDQSP
                     ISMSITADTESERKHLPAANSLNNKLLLADAGYISREYFADVTKAKGSYLVRGSQNLNP
                     KVQAAYRQDGREMPKLLGKKLKDIDRRSCRAEVLDLDVSSGKYKYRLIRRWFAEEKRFC
                     IWMTNLPRERWPAEKVMALYRCRWQIELLFKELKSHNRLKAFATRQKAIAEGLIWASLL
                     ALLVKRRLALTLIGSAELSMLKIAKNSALWLMPILASIIHGAWQEITEKLEWAMVYLSK
                     NGKKSRQRKSKQNNTLDGIINSLAA"
                     /name="IS4-like element ISApu2 family transposase"
     gene            4362..4733
                     /locus_tag="HV105_RS31620"
                     /old_locus_tag="HV105_31620"
     CDS             4362..4733
                     /locus_tag="HV105_RS31620"
                     /old_locus_tag="HV105_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004099020.1"
                     /translation="MTQERIQTRYPLGGPFATQAELEQAITQAITTHRQDFFPLLERLV
                     DVGDTRVNLSQENPLRVLSIELADGREGVAHINYESNFAESCLIINEYDQHQTSLAFRL
                     DGDALIFDVELPIAWNFNN"
                     /name="hypothetical protein"
     gene            5051..6319
                     /locus_tag="HV105_RS31625"
                     /old_locus_tag="HV105_31625"
     CDS             5051..6319
                     /locus_tag="HV105_RS31625"
                     /old_locus_tag="HV105_31625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191342.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu1 family transposase"
                     /protein_id="WP_011191342.1"
                     /translation="MTIINANYYLEQLLAPAALERIARLCKFCLRQRAITPAMLVPALL
                     RAMGGDQVNDIAGLHRHFNALQLVNTHQVSYKPFHNQLRKESFALFMKALVERAIALRI
                     DHQLKAASLGAFKQVLLHDGTSFAVHRRLAADFPGRFKTISPAAIECHMTMSLSEQSPL
                     CMSVSADTAGERQFLPEAHTLTNCLLLADAGYIDRAWFEQVNDAGGFYLVRGTKSLNPK
                     IIQAWRGDGREVPKLAGLSLREAGRRRCRAEVLDMVVKSGQVEYRLIRRWFAEEKRFCL
                     WMTNLPRAAWSAEQVMSLYRCRWQVELLFKEWKSHNRLKGFVTGEKAIAEGLVWTSLLS
                     LVMKRRVAQSVMSGALSMLKASKNSATWWLPLLEAVAHRALTEIRERLEWAADYLAKNA
                     CRTKQRKSIQNRTLEGVLNGLAA"
                     /name="IS4-like element ISApu1 family transposase"
     gene            6352..6468
                     /locus_tag="HV105_RS31630"
                     /old_locus_tag="HV105_31630"
     CDS             6352..6468
                     /locus_tag="HV105_RS31630"
                     /old_locus_tag="HV105_31630"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_072072242.1"
                     /translation="MVLAACQVNNVRDTGGGAVSPSSTVTGVTLDSGTNGNP"
                     /name="colicin release lysis protein"
     Typing          complement(2..290)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.207"
                     /coverage="92.0"
                     /evalue="6.27e-131"
     Typing          complement(6675..6759)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="99.0"
                     /evalue="5.06e-25"
ORIGIN
        1 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
       61 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
      121 ccgaatggca ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct
      181 ttatagtcct gtcgggtttc gccaccactg atttgagcgt caaattctgt gatgcttgtc
      241 aggggggcgg agcctatgga aaaacggccg ctgtgctgcc ttctccctct ccgagctgtc
      301 tgctctatgt cgttatgtgc gcgttgcgac cgctcgccgc agccgaacgg caggagcgta
      361 gcgactgagt gagcgaggaa gcggaaaaga gatctgtctg acattgagca ctgacgctct
      421 gaggcccttt atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa
      481 tcagtctctg ctcctgctgg agaagcttaa cgagcttgat ctcgatgttg aggccgacct
      541 ttgtgagaaa caccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga
      601 tgaattacag gatggtaatt gatggccaga gtgtctatta gtgaagcggc acggcttgtt
      661 aaggttagcc gtcccacaat ttacaagatg attaacagtg ggaagttaag ttacacctct
      721 gttgtaaagc acgggaaggc tattaaagtt attgatactt cagagcttat tagagtgttt
      781 ggttctcttg atggtgtcat cgatactgta aaatatgatg taaaaagtga cgcagaatct
      841 acaagtatta acagcgttgg tttacatgat ttacaacata gaatcgctct acttgaagct
      901 gaaaacgatg gtcttaaagg ggctgttaag gctcgagatg agcatattga ttcccttcgt
      961 caggctatgc agcttctgga gcacaagcgc gagccttctt caccacccca ttccccgtgg
     1021 tggaaattct ggaaaaaatc ctgatcctgc catgcttgag ttttcctctc cggccatccc
     1081 agggagttcc tgaaccttcc caaaaatcgc cagatctctg aacgctcctc acgactaaaa
     1141 atacccacac actacgcaaa attaaaattt tgcagattcc ttatttaaaa caattggtta
     1201 atgaaaaatt aatgtgcagt gatgtgttac aaagtgcaga tccaagtcgt ggacgactct
     1261 atggcgaatc gtttacgaat tgcccacgat tcatatgtga atcacgcttt attatcatgt
     1321 tgttatgatg aatcgactct tgattcaatt catcaatttg ggcgtttttt gttcatggtg
     1381 actcttgatt caattcacga ttcgaatcgc cggtgaatcg tgtggtgagt cgtccggcga
     1441 cttgccataa cgtgattgcc ggccgtcgac gaacccgatt cctgaacgtg ctgaaaaccg
     1501 gttttccggc tgcgccggca acgcctcacg gccacgcaat aaattgcgtc cccgttcacc
     1561 gttttcggat cagaagaacg ccggaaacag gacttaccct ggctgaatgg cgtgaatttc
     1621 cgcgctatgc acttgcgcgc atactcatgc atgccgtaaa aacagagcct gcgcgtttct
     1681 ggcgggtttt cgggtggttt gttgcctgtt ttaccggttt cccgtcagaa acgtcctgag
     1741 gccgttttag cggtgcgtac aattaaggga ttatggtaaa tggtgacttg gttaatcata
     1801 aacatcctgg gggatggcat acaccagttg gatatccggt gcatcgttga gccatcgacc
     1861 tgattttttc attaagtagc caccaatttc ttcggtagtc agccgggcat gaaacaatgc
     1921 ttcatcattg aaccagcagt cagggtgata gatgagatcc agtgggtttg ccttgaaatt
     1981 aatctccagt aactggagtg catagctgtg gtcagatgca ctaccttctg cttttcgtat
     2041 ggcggatgac ctcgattttg acgatattat tctggtgttt cctccggaat ccggcctaaa
     2101 accgctgtat gtgatgtacc gtagccctcg taacatgccc ggtacagtga gcggcaaagg
     2161 gcagaacgtt ggcaataact ggatgggggg ggccagtacc ggggatggtg ctcctgttcc
     2221 ttcccagatt gcggataaat tacgcgggaa gactttcggt agttttgatt ctttccgtcg
     2281 ggcgttctgg aaagcggttg ctgacgattc tgcactgagc aagcaattta gtgaagctga
     2341 tattaatcag atgaaagctg gaagggcacc tactgcggac tttttagaat cagtagggaa
     2401 acgtgttaaa attgaattac accacgaaaa agaaatatct caaggagggg ctgttatgga
     2461 tgtcgataac attaaggcgc taacacctaa aaatcatatt gagacacata aaggaaaata
     2521 aaatggctgg taataaaatt tcagatttta cggaagaaga atttactgct tttgttagta
     2581 aaataatcgc tgctgatttt ccaactgaca aagagaacga tgatgctatt tattcgttcg
     2641 cccaactaac agagcatcct gctggttgga acctcatctt caagcctaag gtaggcgaag
     2701 attcttcggc gaaaggaatt gttaaaaccg taaaggaatg gcgagctgct aacggtaagc
     2761 caggttttaa aaaagcgtga ttgaatttgc gggttcaagt tatgagcccg ctcatttgta
     2821 actctggatt tgaagaataa ttactagata tccatttgcc tagaataata aaaataaaag
     2881 gtgatatatg aaaaaattac ttttcctagg ggttgttcat gtacagaacc ttaagcggca
     2941 agcgaattaa taataccgtc taacgtgttg ttttgcttgg attttctctg cctactcttt
     3001 ttgccgtttt tggataaata caccattgcc cactccaact tctctgttat ttcctgccac
     3061 gcaccatgga tgatgctcgc caatatcggc attaaccaaa gcgcgctgtt tttggcaatc
     3121 ttcagcatcg ataactccgc gctgccaatc agcgtcagcg ccagccgacg tttgaccagc
     3181 aaggccagca aactagccca gatcaagcct tccgctatcg ctttttgtcg tgtcgcgaac
     3241 gccttcaacc ggttatgtga tttcagctct ttgaacaaca gttcgatctg ccaacggcaa
     3301 cgatacaatg ccatcacctt ctcggctggc catcgctcac gcggaagatt ggtcatccat
     3361 atacagaacc gtttttcctc tgcaaaccaa cgtcttatga gtcgatattt atactttccc
     3421 gaactcacat ccagatccag aacctcggct ctgcagcttc tccgatcgat atccttgagc
     3481 ttttttccga gtaacttggg catttccctg ccgtcctgtc ggtaagctgc ctgtactttg
     3541 ggattgagat tttgactccc tctgaccaga tagcttcctt tggctttggt cacatcagca
     3601 aagtactcgc ggctgatgta accggcatcc gccaataaca gtttgttatt taacgaattt
     3661 gcggccggaa gatgcttgcg ctccgattcg gtatccgccg tgatactcat cgatatcggc
     3721 gactggtcca gcaaggacat ggtcatatga cactctaccg cagcggggct gtgcttattg
     3781 aatcggcccg ggaaatgttc tgccaattgc ggatgtaacg cgaatgacga cccgtcctgc
     3841 agcagtacct gctcaaagcc tgccagtccg tgggcgtccg gtagacactc cttgaggcgt
     3901 aaggcgatgg ctcgttcaac cagtgcgcgc atgaacaagg gaaaaccctg cttgcgtaac
     3961 tggttgtgaa agggtttata cgctatttgg tcagtatcat ggagctgcaa cgcattgaaa
     4021 tggcgatgca aaccagcaat accatccacc ttgtcgcaac caagccctga gagcagagcc
     4081 gggagtagtc tcgcaggctg gatatcccgg agtcgctgac aaaaaccggt aaatttaccg
     4141 atccggttga gttcagaaga gctaagaagt tgcttgaggt actgggttgc attgataata
     4201 gtcatggctt cgggcgtgtc ggtttttttt tgcgattaaa tcagatcaca aacgaagcct
     4261 tttttctatc tatttcaatt ggttgtagct aaagatcctg tacatgacag gtcactgcag
     4321 cggccaaact gttgccacat ctcagttcag gaggaaacgc aatgactcag gaaaggatcc
     4381 agacccgcta cccactcggc ggccccttcg ccacccaggc tgagctggag caggccatca
     4441 cccaggccat aacgacccac agacaagatt tcttccctct gctggaacgg ctggtggacg
     4501 tgggggacac ccgggtcaac ctcagtcagg agaacccgct gcgggtgctc agcatcgaac
     4561 tcgctgacgg ccgtgaagga gtggcccata tcaactacga gtccaacttc gccgagagct
     4621 gcctgattat caacgaatac gaccagcacc agaccagcct ggcctttcgc ctcgacgggg
     4681 atgcacttat cttcgacgtc gaactgccga tcgcgtggaa tttcaataac tgaagattca
     4741 gcggtgtaat gccgcctggt tgctacaccc ggtcagccag ctcccctcac cctaaccctc
     4801 tcccaaaggg agaggggatc agcccgtgcc aacctccagc cgtggtagtc actccatact
     4861 ctgctgttat ttcaggcgcc tgtaatcttc cccatatggc tgtcatctga atagcaatat
     4921 ccagcatata gccaccataa ccctcactca tgtacaggat ctttagcggc aaccaattga
     4981 aatagatata aaaaaggctt cgtttgtgat ctgattttat cgccaaacaa aatcaacacg
     5041 cgcgaagcct atgactatta tcaacgctaa ttactatctg gagcaactct tggctcccgc
     5101 cgcactcgag cgcattgctc ggctctgcaa gttttgtctg cgtcagcgtg ccattacccc
     5161 agccatgctc gtacctgcac tgctgcgggc catgggaggc gaccaggtca atgacattgc
     5221 cggtttacac cgccatttca atgccttgca actggtaaac acccatcagg tttcttacaa
     5281 accgtttcac aatcagctgc gcaaagagag ctttgccctg tttatgaaag ccctggtcga
     5341 gcgggccatc gccttgcgta tcgaccatca actcaaagca gcctccctcg gcgcgttcaa
     5401 gcaggtgtta ctgcacgatg gcacctcctt tgcggtgcat cggcgccttg ctgctgattt
     5461 tcccggccgg ttcaagacca tcagtccggc agctatcgag tgccatatga ccatgtcact
     5521 gtcggagcag agcccgttgt gcatgagtgt gtctgccgat accgcagggg aacggcaatt
     5581 tctgcctgaa gcccacacgc tgaccaactg cctgctgctg gctgacgcag gttacattga
     5641 cagggcttgg tttgagcagg tgaacgacgc tggcggattt tatctggtgc gcggcacgaa
     5701 aagcctgaac cccaagataa tccaagcctg gcgcggtgat ggacgggaag tacccaagct
     5761 ggcgggacta tctctgagag aggccggacg gcgacgctgt cgggccgagg tactggatat
     5821 ggtggtgaag tccggccagg tcgagtaccg gttgatccgg cgctggtttg ctgaggaaaa
     5881 gcggttctgt ctgtggatga cgaacctacc gcgagcagca tggtcagcag agcaggtgat
     5941 gagcctgtat cgctgccgtt ggcaggtgga gttgttattt aaagagtgga aatcgcacaa
     6001 ccggctcaag gggtttgtga cgggcgagaa agcgattgcc gaggggttgg tgtggacgag
     6061 cctgctgtcg ctggtgatga agagacgggt cgcgcagagt gtgatgagtg gggcgctatc
     6121 gatgctgaaa gcgtcgaaga acagtgcgac gtggtggcta ccgctattgg aagcagtggc
     6181 ccatagggcg ctgacagaaa taagggaaag attggaatgg gctgccgatt atttggccaa
     6241 gaacgcctgc cgaaccaagc agagaaagtc gatacagaac aggaccttgg aaggcgtttt
     6301 aaatggactc gccgcttaag gtcctgtaca tgaggggttg ttaccgtttc tgtggttctg
     6361 gcagcatgtc aggtcaataa tgttcgagac accggaggtg gggctgtttc accttcatcg
     6421 acggtgaccg gagtcacgct tgatagcggt acgaacggca atccgtaaag gatcttcttg
     6481 agatcccatt tggatcgtcg taatctcttg ctctgtaaac gaaaaaaccg ccttgtaggg
     6541 cggtttttcc gaaggttcaa acacttgcag gtgattgaac caaggtaact ggcttggagg
     6601 agcgcagtca ccaaaacttg ttctttcagt ttagccttaa caggcgcatg acttcaagac
     6661 caactcctct aaatccgtta ccagtggctg ctgccagtgg cgctttgtcg tgtctttccg
     6721 ggttggactc aagatgatag ttaccggata agacgcagca g
//
