LOCUS       NZ_CP058116             4479 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00181 plasmid pRHBSTW-00181_5,
            complete sequence.
ACCESSION   NZ_CP058116
VERSION     NZ_CP058116.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148529
            Assembly: GCF_013624225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00181
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.8231516532439x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624225.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 05:09:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,865
            CDSs (total)                      :: 5,749
            Genes (coding)                    :: 5,630
            CDSs (with protein)               :: 5,630
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 87 of 119
            Pseudo Genes (internal stop)      :: 30 of 119
            Pseudo Genes (multiple problems)  :: 42 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058116.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00181"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00181_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            205..396
                     /locus_tag="HV112_RS29175"
                     /old_locus_tag="HV112_29180"
     CDS             205..396
                     /locus_tag="HV112_RS29175"
                     /old_locus_tag="HV112_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_155006913.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDFDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            396..797
                     /locus_tag="HV112_RS29180"
                     /old_locus_tag="HV112_29185"
     CDS             396..797
                     /locus_tag="HV112_RS29180"
                     /old_locus_tag="HV112_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087834902.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMIDTSE
                     LLRVFGPFEQPRGEHHSGQVSEQRDTAFADQSEQVIHHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1607..2512
                     /locus_tag="HV112_RS29185"
                     /old_locus_tag="HV112_29190"
     CDS             1607..2512
                     /locus_tag="HV112_RS29185"
                     /old_locus_tag="HV112_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032490083.1"
                     /translation="MDILKEKIDVASRLYNLNLDHIPATLQVIEHAMLLLKNNAGYGYF
                     GSFNGKNTQEYHSFTFNGEYSRPVRDDLFITDYDFFVSGFREFNESLRDIGSKWSSFDS
                     RRANKIIYTSVMSVACCFDLWKSGSRKTPGTFFEIFMAAVLKWMIPDEIFSKHIPLIDQ
                     LESDDESIDPSSVSTDIVIKSKYKNASVVIPLKITTRERIVQPFAQQRILDSYFGNGVY
                     FSFLACISETQQDKKKKKVNHICVPGTIRLYQKYLSSLSGMYYCDIPERYLERDLTDII
                     PVRTMGDFLFDIYSFFRSQG"
                     /name="hypothetical protein"
     gene            complement(2496..3623)
                     /locus_tag="HV112_RS29190"
                     /old_locus_tag="HV112_29195"
     CDS             complement(2496..3623)
                     /locus_tag="HV112_RS29190"
                     /old_locus_tag="HV112_29195"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758058.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_181333272.1"
                     /translation="MPHINTSNKLTCIEGFCGAGGMSLGLKQAGFDVRLAFDINENAVE
                     TYNKNIGNHCEKLDASIVSGKFLLEKAGIKTRLDLFSGGPPCQGFSKQRRGAHLLDDDR
                     NKLVLEYSRLVNELKPRAFLFENVEIFGQKRGKDLIDEIKIKLFDYNIYTYFICSSDFG
                     VAQKRGRFLMIGIDKSEGKIVPKLLKSDKTLTIRDVIGQLPPPPEDYKEHPDIPNHIKC
                     KITKINEERFSHVPPGGGWKDIPYDLRLKCHQKVDTASGGWPDVYGRLEWDKQCPTITA
                     GFDSFTRGRYGHPSQNRAITLREGAMLQGFPVNYRFYGNRDAIRLQIGNAVPPPVSYAA
                     GTAIINCLDTKQDKNHFNFQNPMELDKDGQFSLAI"
                     /name="DNA cytosine methyltransferase"
     gene            3780..3980
                     /locus_tag="HV112_RS29195"
                     /old_locus_tag="HV112_29200"
     CDS             3780..3980
                     /locus_tag="HV112_RS29195"
                     /old_locus_tag="HV112_29200"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_235606871.1"
                     /translation="MAGIATPTIADFERGARKPHGNNLRSIIIAFENKGLDFVEEGGEI
                     IGIFIRRKNVRAEESIDLSGH"
                     /name="hypothetical protein"
     gene            complement(4155..4268)
                     /locus_tag="HV112_RS29200"
                     /old_locus_tag="HV112_29205"
     CDS             complement(4155..4268)
                     /locus_tag="HV112_RS29200"
                     /old_locus_tag="HV112_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_155006912.1"
                     /translation="MHEPPVQSDYHACSGNYQLESNPERHDKAPLAVAIGN"
                     /name="replication initiation protein"
     Typing          complement(4156..4479)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="87.0"
                     /evalue="1.36e-127"
ORIGIN
        1 gtcagggggg cggagcctat ggaaaaacgg caacgccgcg gccttctttt gttccttctg
       61 gccctctgtg ctgtcgtctt atgcccttat acgtgagtta ctaccgctcg ccgcagccga
      121 acggcaggag catagcgact gagtgagcga ggaagcggaa aagagaccag ttttacattg
      181 agcactgacg ctctgaggcc ctttatgact aaacaacaga aaacagcgct gaatatggct
      241 aaattcatcc agaatcagtc tctgctcctg ctggagaagc ttaacgagct tgattttgat
      301 gctgaggccg acctttgtga gaaactccac gatgatgcgg agcatctttt ccgtacactg
      361 tcctcgagac tggatgcatt acaggatggt aactgatggc cagagtgtct attagtgagg
      421 ctgcccgact gacgggtaag agtagaacaa cactgcacag gttaattaaa gcaggtgacc
      481 tgtccacctg tagcggtgaa cgcaatgcaa aaatgataga tacttcagag cttttgcgtg
      541 tctttggccc ctttgagcaa ccgaggggtg aacaccattc tggacaggtc tctgaacagc
      601 gtgatacagc ctttgctgac cagagtgaac aggtgattca tcatctgaaa caggagattg
      661 aacacctgaa taccttactt ttgtccaagg attcgcatat tgacagcctg aaacaggcca
      721 tgctccttct tgaaaatcac caggcaaaat taactgaatc cccggcgcca tggtggaaat
      781 tctggaaaaa atcctgatcc cgccatgctt gagttttcct ctccggccat cctggggagt
      841 tcctgaacct tcccaaaaat cgtcagatct ctgaacgctc ctcaccacga aaaataccca
      901 caccccacgc aaaattaaaa ttttgcagat ttatatttaa tatcagtagt ttactttcct
      961 gaaatttcgt tttttaaatt ttatttatat agatccgact ctatgacgag tcgtcataga
     1021 gtccccggcg agtcgtcata gagtcggtaa cgactcagga ctataaaaac aatgagttaa
     1081 taacgacccg actctagacc cgactctccg tttggttgtt ttttagacgc ggcgactcta
     1141 ggtacgactc tagtgaagag tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc
     1201 attacgtcat tgccggccgt cgatgaaccc gattcctgaa cgtgctgaaa aggggttttc
     1261 cggctgcgcc ggcaacggct cacgaccacg caataaattg cgtccccgtt caccgttttc
     1321 ggaggagaag aacgccggaa acaggacttc ccctggctga atggtgtgaa tttccgcgct
     1381 atgcacttgc gcgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg
     1441 ttctcgggtg gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc tgagggcctc
     1501 tcaggcggtg ctgataacgg atgttatggt aaatgaaagt agctctattt atatgaaaaa
     1561 cattcactaa taaacaaaat taacgcagaa aaggcatggg aaaaacatgg atatcttgaa
     1621 agaaaaaatt gacgttgctt cacggcttta taatttaaac ttggatcata ttcctgcaac
     1681 actgcaggta atagaacatg caatgttgtt attaaaaaac aatgcaggat atggttactt
     1741 tggttcattt aatggaaaaa atacacaaga atatcattca tttacgttca atggcgaata
     1801 ttcaagacca gtaagagatg atctctttat cactgactat gatttctttg tttcagggtt
     1861 tagagaattt aatgaaagtt tgagggatat tggtagtaaa tggtcatcct ttgattctag
     1921 gagagctaat aaaataattt atacatctgt aatgtctgtt gcatgctgct ttgatctttg
     1981 gaaaagtggt agcagaaaga ctcctggaac attttttgaa attttcatgg cggcagtgtt
     2041 gaaatggatg attcctgatg agattttctc gaagcacata ccgctaatag accaacttga
     2101 atcagatgat gagtcaatcg atccatcaag cgtgtctact gatattgtta taaagtctaa
     2161 atataaaaac gctagtgtag ttataccttt aaagataact acaagagaga ggatcgttca
     2221 accatttgcc cagcagagaa tattggactc atactttgga aatggtgttt atttttcttt
     2281 cttagcgtgt attagcgaga cgcaacaaga taagaagaag aaaaaggtta atcatatatg
     2341 tgttccaggg acaataagat tatatcaaaa atatctctcc agtctctctg gtatgtatta
     2401 ctgcgatatt ccagagcgtt acttggaaag agatctgacc gatataatac cggtcagaac
     2461 tatgggtgat ttcttatttg atatttactc tttctttaga tcgcaaggct aaattgtccg
     2521 tctttatcca actccattgg attttggaag ttaaagtgat ttttatcttg cttcgtatct
     2581 aaacaattaa ttattgctgt tcctgctgcg taggacactg gcggtgggac agcatttcca
     2641 atttgtagcc taatagcatc acggttacca tagaatctat agtttacagg gaatccttgt
     2701 agcattgcgc cctcccttaa tgttattgct ctattttgtg aggggtgtcc atacctgcct
     2761 ctagtgaaac tatcgaagcc tgctgttatg gttggacatt gcttgtccca ttcaagtctc
     2821 ccataaacgt caggccatcc tcccgatgcc gtatcaacct tttggtgaca ttttaacctt
     2881 aagtcgtatg gtatatcttt ccatcctccg ccaggaggga cgtgtgaaaa cctttcctca
     2941 ttaatctttg ttattttaca ttttatatga ttaggtatgt ctgggtgctc tttataatcc
     3001 tctggtggag ggggtaactg accaattaca tctcttattg taagggtttt atctgatttt
     3061 aatagtttgg ggacgatttt accttctgac ttgtctattc caatcattag gaaacggcct
     3121 cttttttgcg caactccaaa atcagatgaa cagatgaaat atgtgtatat gttataatca
     3181 aacagcttga tcttaatttc atcaataaga tctttgcctc ttttttgacc aaatatttcc
     3241 acattttcaa ataaaaaagc cctaggtttt aattcattaa ctaacctaga atattcaaga
     3301 actaacttat ttctatcgtc atcaagaaga tgggcacctc ttctttgttt tgaaaacccc
     3361 tgacaaggcg gcccaccact aaaaagatct agtctcgttt ttatgcctgc tttttccaaa
     3421 agaaacttcc cgctaacaat agaagcatct aatttttcgc aatggttacc aatgtttttg
     3481 ttgtaggttt ctacagcatt ttcattaata tcaaatgcca acctaacatc aaaacctgct
     3541 tgtttcagac cgagggacat acccccggca ccacaaaaac cttcgatgca cgttagttta
     3601 ttagatgtgt ttatatgtgg catgatgtct cacttacaag gggccgttta catgtatgat
     3661 agcgttaatt atacctagag gtcaatgttt tgatacaaaa tcatattaaa aatatgactc
     3721 ctgaaatttg taaggcctct agggccttag ttaacttgac tcagaaagag ctagctctta
     3781 tggctggaat tgcgacacca accattgctg attttgagcg tggcgcaaga aaacctcatg
     3841 gcaataactt gagatctata attattgcct ttgaaaacaa ggggctggat tttgttgagg
     3901 agggtggtga gataatcggg atctttatca gaagaaagaa tgtcagagcc gaggaatcta
     3961 ttgatctgtc aggtcattga gggcatcatc ttatcggaaa gaaaaaaccg ccctgcaagg
     4021 cggttttttc aaaggttctc tgagctagca actctttgaa ccgaggtaac tggcttggag
     4081 gagcacagtc accaaaactt gtcctttcag tttagcctta accggggcat aacttcaaga
     4141 ctatctcctc taaatcagtt accaatggct accgccagtg gcgctttgtc gtgtctttcc
     4201 gggttggact caagttgata gttaccggaa caggcgtggt agtcggactg aacggggggt
     4261 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
     4321 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     4381 agagcgcacg agggagccac cagggggaaa cgcctggtat ctttaaagtc ctgtcgggtt
     4441 tcgccaccac tgatttgagc gtccgtttct gtgatgctt
//
