LOCUS       NZ_CP058115             4667 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00181 plasmid pRHBSTW-00181_4,
            complete sequence.
ACCESSION   NZ_CP058115
VERSION     NZ_CP058115.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148529
            Assembly: GCF_013624225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00181
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.8231516532439x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013624225.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 05:09:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,865
            CDSs (total)                      :: 5,749
            Genes (coding)                    :: 5,630
            CDSs (with protein)               :: 5,630
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 87 of 119
            Pseudo Genes (internal stop)      :: 30 of 119
            Pseudo Genes (multiple problems)  :: 42 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058115.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00181"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00181_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            532..1017
                     /locus_tag="HV112_RS29155"
                     /old_locus_tag="HV112_29160"
     CDS             532..1017
                     /locus_tag="HV112_RS29155"
                     /old_locus_tag="HV112_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1021..1236
                     /locus_tag="HV112_RS29160"
                     /old_locus_tag="HV112_29165"
     CDS             1021..1236
                     /locus_tag="HV112_RS29160"
                     /old_locus_tag="HV112_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1582..3138
                     /locus_tag="HV112_RS29165"
                     /old_locus_tag="HV112_29170"
     CDS             1582..3138
                     /locus_tag="HV112_RS29165"
                     /old_locus_tag="HV112_29170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_045326453.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            4198..4521
                     /locus_tag="HV112_RS29170"
                     /old_locus_tag="HV112_29175"
     CDS             4198..4521
                     /locus_tag="HV112_RS29170"
                     /old_locus_tag="HV112_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3913..4085
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(3436..3988)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          4..1340
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.256"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4199..4515
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          4512..4666
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.769"
                     /coverage="82.0"
                     /evalue="1.55e-13"
ORIGIN
        1 acacctccgg ggtactgtcg tttgcggaag cggatttacc ctccggccag cgggaaaagc
       61 tgatggcaag ctttgagcgg gttctgatgc ccggacttga taaagaccag tacagcatcc
      121 tgtgggtgga gcatcgtgac aaggggcggc ttgagctgaa ttttctgatc ccgaacacgg
      181 agctgctgac cggcagacgg ctccagccgt attacgatcg cgccgaccgt ccacgcatcg
      241 atgcctggca gaccatcgtg aacggcagac tggggctgca cgacccgaac gcaccggaga
      301 accggcgggc actggtcaca ccgtctgcac tgcctaaagc gaagcaggag gctgcagaag
      361 cgattacacg cggtctgctt gctcttgcct catccgggga gctgaaaacc cgtcaggatg
      421 tcactgaggc gctggaaagc gcaggttttg aggtggtccg caccacaaaa agcagtatca
      481 gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagccatt tatgaacagt
      541 cttttaacgc tggcgaagga cttagagcag aaatcgaaag cgcagcagca gagtaccggc
      601 gagatgctga aagccgcatt cagcgagcac gagaaatctg taaaagcgga actgaacgaa
      661 agcgcgaaga gaatcagcgc cgccatcctc gaccacgacc ggaaactgtc ctcagccatg
      721 agccagcgca cgaaaggcat ggtgcgcatg gtcagccaga cgtggctgac catcgtcctg
      781 gtctccgcgc tgctgatcgc ctcgagcgcg ggcattctgt ggtggcaggg gcagcagata
      841 ctcgacaact acacgaccat ccgggagcag aagagcacgc aggccatgct gtcagagagg
      901 aacagcggcg tgcagctctc gacctgcggc gagcagagac gccgctgcgt gaaggtgaac
      961 ccggaagcgg gacggttcgg agaggattcg agctggatga tactggcggg gaaatagcac
     1021 atgacggagc tggaaaaaca gttgctgagc gcattagagc agctacagca ggactactcg
     1081 aaaaggctgg acgagtggga gagcgccttc gcggaatggc ggacgatgtc tggtcttatg
     1141 caacgggaga acgcagcgct gagcgagcgc gtcacgggct tgagcaggca ggtgcagagt
     1201 ttgagcgagc agctgcgccg gttgtcgagc gactgaatgc cgtcgagctg caccgggagc
     1261 aggagcgcgt ggtgcagcat cagaaagcgc tggagctgga gcgatctcag cgacagcgga
     1321 cgtataacag cccttcactg tgataacctg ataaaacggg gctttcggtg gacatctgcg
     1381 ctgaaagccg gagccattca cccgactgca tagccatgca gccgggtgaa atttatctgg
     1441 cgcagaatgc gcggtttagc ggagagtttg ctgccggttt taccgggctg acgccgcatt
     1501 tgttccgaac ccgtccgggg cggtgctgat aagggatgtt atggtaaatg cggcattttt
     1561 tattatatgg agtgtatttt tatgagaatt aaaaagcctc accttcgtcc gatagtcata
     1621 accttgtccg ttggtgggat tttgttaact tctatttttc tgattgccgt tattatggtt
     1681 tttcagcgcg aaaatataga aaaagatttg cttgatgcta attcatcgta cgctatgaaa
     1741 atgtcagatg taatgagtgg ttatattgaa atggctcaag gtgaattagc atatggagca
     1801 aaaaaaataa aatcaactaa tgatattgac tcacttaaag atgaggcgga taggttaaga
     1861 ttgcagtctg gtatgtttaa ttcagttttt gttgttagta atgatgctgt tgttctggcg
     1921 acttcgccag aatctcttga tttagttggt acccgcttga attcaagtgc cagcaaattg
     1981 gcgattgagg aaaagaaacc attcatatct tcaccgttca aatcagttac tgggaatttg
     2041 attattttgt tgtcacatcc tatttatgat aagaatggca attacatagg gcatgtcggt
     2101 gggtctatat atctaaaaaa acatagttta tttagcgatg ttttgagcag gcattttttc
     2161 aagagcgaca ctgaaattag cattgttagt gatgatggaa ctgtgatctt taataacgat
     2221 aacagtgctg ttggtcggcc catggttatg tctgatgatt tgaaatcaaa gttaacggag
     2281 tctgatagag gtgaggggga attttttttt tcggggaata gatatcttct gggttatgct
     2341 catatgaaaa atactgcttg gaatgtgttg gtttatagcc atgctgacag cgcgactact
     2401 attcttatta cttatgctaa aagtgtactt gttctgctaa ttgtaataat tattacattt
     2461 tcacttcttt catatttcat tgcctcccgt atttcacttc ctttagaaaa gcttgctata
     2521 tctaccaatg ctaaagatat aggggcttcg ttagtttata ttaaagctat caatgcttgg
     2581 tatgccgaag ctgatcgact taaaaaggca ctttttacgc atgttaaggt aatgatggaa
     2641 agggttgatt ctttaaatga ggtggcgctt aaagatcccc ttaccggcgt gaacaataga
     2701 ttggggtttt caactaaaat acagaataac aagtatgggg caggagcttc agttattgct
     2761 attgatgttg atttttttaa aaaaataaat gatagtttcg ggcatggggt cggagatgag
     2821 gtgcttgttt ctttggctca gattattaag tcttgttgcc gtagtgaaga tattatttgt
     2881 cggtttggtg gagaagagtt tgttgttttt ctgccgaata catctgtggt aacagcggag
     2941 tgtgttgcgg agcgtattag gagtgttatt gaacgtactg ttttccctaa taacttgcgt
     3001 gtcaccgtat ctgctggtgt ggcaacacaa gatgatcctc ttggtgggat cgattttttg
     3061 ctgaaaaatg ctgacgatgc tctttatcaa gctaagagag aggggcgtaa taaagtgatt
     3121 gtatatagcg ctatttgagt ttttttgatg acgctttacg cgctagtgtg ccgttttttt
     3181 ttgtggggag gggggttcaa gaagcggatc ctcttgttat cccttcgatg tcgtcgtaat
     3241 ctcatgccct gcaaacaaaa aaaccaccgc taccaacggt ggttcgtttg ccggaatcaa
     3301 gagctaccaa ctctttcttc cgaaggtaac tggctttcac ggagcgcagg taccaaaact
     3361 gtccttccag tgtagccgca gtcaggccac cacttcaaga actctgtaga acactctact
     3421 actccgctcc gtgaatcctc ttactaatgg ctaccgccac tggcgatttt tcgtgtctgt
     3481 tccgggttgg actcaagatg atagttaccg ggacaacgtg gtagtcgggc tgaacggggg
     3541 gttcgtgcat acagtccagc ttggagcgaa ctgccttccc ggaaccgagt gtcaggcgtg
     3601 gaatgagata aacgcggcca taacagcgga ttgacaccgg taaaccgaaa ggcaggaaca
     3661 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
     3781 tggaaaaacg gcttcgccgt ggccttatcg cttccctgtt aagcttcttc ctggcatctc
     3841 ccaggaaatt tccgccccgc ctgtaagccg gtaccgctcg ccgcagccga acgaccgagc
     3901 gcagcgagtc agtgagcgag gaagcggaat atatcttgta tcacatattc tgctgacgct
     3961 ccgctgctgc atttttctcc tgccacatga agcatttcac tgacactcgc atccgtgcca
     4021 acatagtaag ccagtataca ctccgctagc gctacgtgac tggttcaggg ctgcgccccg
     4081 aaacccgcca aaagcctgac gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg
     4141 ctttcccgcc cgtcccggcg ttatcaggag gccggtttgt cagagaaacg gaacaagatg
     4201 ctcaccatgt gggtgacgga ggacgagcac cggcggctgc ttgagcgctg cgacggcagg
     4261 cagctggcag cgtggatgcg gcagacctgc ctggacgaaa agccggcacg ttccggaaag
     4321 cttccctcaa tctcgccggc gctgctgcgc cagcttgccg gcatgggcaa taacctgaac
     4381 cagatagccc gccgtgttaa cgccggcggc ggtaccggcc atgacagggt gcagattgtt
     4441 gcggcgctga tggccatcga tgccggactt gagcggctgc ggcacgccgt tcttgagaaa
     4501 ggaacggacg atgatcgtta aatttcatcc acgcggtcgc ggcggtggcg ccgggccggt
     4561 ggactatctg ctgggtaaag accggcagcg tgaaggcgcc accgtcctgc aggggaagcc
     4621 ggaagaagtc cgggagctga ttgacgcgtc gccttacgtt aagaaat
//
