LOCUS       NZ_CP057898             2064 bp    DNA     circular CON 02-APR-2024
DEFINITION  Escherichia fergusonii strain RHB10-C23 plasmid pRHB10-C23_3,
            complete sequence.
ACCESSION   NZ_CP057898
VERSION     NZ_CP057898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148066
            Assembly: GCF_013899685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH15 T1-C23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.796543705692x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013899685.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 04:33:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,555
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 4,303
            CDSs (with protein)               :: 4,303
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 46 of 137
            Pseudo Genes (incomplete)         :: 80 of 137
            Pseudo Genes (internal stop)      :: 20 of 137
            Pseudo Genes (multiple problems)  :: 11 of 137
            Pseudo Genes (short protein)      :: 2 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP057898.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB10-C23"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB10-C23_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1186..2064,1..111))
                     /locus_tag="HVW49_RS22640"
                     /old_locus_tag="HVW49_22645"
     CDS             complement(join(1186..2064,1..111))
                     /locus_tag="HVW49_RS22640"
                     /old_locus_tag="HVW49_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_181576157.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLSRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          684..765
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="96.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(1097..1183)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.182"
                     /coverage="96.0"
                     /evalue="9.44e-28"
     Typing          1269..1460
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 ggccgcaagc aggtaaatcc ctcccacgtc gtcagaaacg cttctgtgtg cgtcccaggg
       61 cgcatcagag ggggaataat cagtgaggta ctggagttta gggttttgca tgcacaccgc
      121 cccatactgg gcgatggctc gattgacgtg ttatcattgc catatcacct gttgctctca
      181 tcatgaatcc agtttctgca ggtggtcaca cccccgaacg atgcccgtcg ttgcgggggt
      241 cacttttaac gcaaccactc ccgacagaat aactccaaaa ccattttccg acaaatcccc
      301 cctcaggcct tgcggcacaa gggctggagg cgaatttgaa tcctagagtt atcccttaag
      361 tataaataca aggatcacgc gcgagatttg cttgaatttt gagctggatt ttcagtaacc
      421 aaaaacctcc caaccccacc cacttacacc catctcatcc acagactttc accccgattt
      481 tgtgaacaac aaaaaaccgt cttgcaggtg gttggcctca ctccgtgaca ccaaccacca
      541 tccccagtgt tttccccgtg ccgtcgtcaa tcacaaaatc ggaacccacg ccagaggcgc
      601 ggggccccgc gattttctgt ttgacgagtt cgggaacggt gagcggtacg cggaattttt
      661 tcgcgggccg tgaggcgttg gcggggtgtc ggggcgcagc cctgaccagg tggtaattgt
      721 gatagcgtcg cgtgtgacgg tattacaatt acacatcctg tcccgaaaat tcccgccgtt
      781 tctcggtttt ttgagcgtcg cagaccgcac gacaaacgaa acgaagtgaa gtttgtataa
      841 gtgcgcactt cgtgagattc cccgactgcc agtttgtcga tcgcgcggaa cggctgcggc
      901 acaaaaacgg caaaatgatt tgctgtccgt tcgccgaaat ttttgcgtag ctgtggtggc
      961 tgcggtgcca aaaatcatcc gcgcgttaag tgtggatgcg cacttagtag tttctctcct
     1021 tcgtcgggaa actgtgcgct cctgcggagg ggttaacggc gcagaactgc cgatgctctg
     1081 ccagcgttct ggcgaaaaaa tcgggacagg atgtgtaatt gtaataccgt cacacgcgac
     1141 gctatcacaa ttaccacctg gtcagggctg cgccccgaca ccctgctagt ctgattcagt
     1201 tttatctttc gtcggtgagc ggcggtactt tctgacctcg gttttccatt cgaacgcgat
     1261 gcgtgaaccg tcatcgtcgc cctcggaaat atcatctccg atcaccatgt cggcgtcggt
     1321 ttcctgatcg agtttcagca catctttgag tgcgccgccg gtcgccacaa agcggcgctt
     1381 gtgcgtctgg cgagtcagtt cgaggaacca ttcaggatcg gccaccatgt cagccggctt
     1441 cgtggaatat ttcagggttt cggccactgc gccgcgcacc agttcagcgg tcgcgcaggc
     1501 taaggactcg ccatccttcg gcttgcgggg tttcactgcc cggacatcaa cattcgggtc
     1561 ataatcaacc cgcaggcact cccgccatag ctcaacccag cgttcatgcc tgacgtaact
     1621 tttcccattt agcatgccag gaggagccat catcaaggta tggaagtgcg ggtgtgcgct
     1681 gccgtcgctg ccgcgggtaa cttccgttgt gcggatccag ccctgaacag gcaggaactc
     1741 cttgcgaacc ttcatacgct gaaaagctgc attcatccgg ttcagcatct cgcccagttc
     1801 cccaatcgca cagttgcgca cggtcagggt caggaacatc cagcgggcat cgggataatc
     1861 cgccacgata cgcgacaggg actggtagaa acgggcttgc cacatcaggg agcgcctcca
     1921 ctggcagaca ggacagtgcc ggacacggca gaaatgtgcc tcacgcagcc tcagacgcgt
     1981 ttctccggtt tccttgaggg tagaccaccc gaaacgcagt aaaccgccac aggaagccat
     2041 acgagccccg tagtgctcat attc
//
