LOCUS       NZ_CP057360             6669 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia fergusonii strain RHB28-C21 plasmid pRHB28-C21_4,
            complete sequence.
ACCESSION   NZ_CP057360
VERSION     NZ_CP057360.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148274
            Assembly: GCF_013829265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T2-C21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.972283596324x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013829265.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:13:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,953
            CDSs (total)                      :: 4,834
            Genes (coding)                    :: 4,677
            CDSs (with protein)               :: 4,677
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 36 of 157
            Pseudo Genes (incomplete)         :: 109 of 157
            Pseudo Genes (internal stop)      :: 22 of 157
            Pseudo Genes (multiple problems)  :: 12 of 157
            Pseudo Genes (short protein)      :: 2 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP057360.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB28-C21"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB28-C21_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5811..6669,1..710)
                     /gene="cea"
                     /locus_tag="HVY57_RS24435"
                     /old_locus_tag="HVY57_24385"
     CDS             join(5811..6669,1..710)
                     /gene="cea"
                     /locus_tag="HVY57_RS24435"
                     /old_locus_tag="HVY57_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1096..1233
                     /locus_tag="HVY57_RS24445"
                     /old_locus_tag="HVY57_24395"
     CDS             1096..1233
                     /locus_tag="HVY57_RS24445"
                     /old_locus_tag="HVY57_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(1432..1590)
                     /locus_tag="HVY57_RS24975"
     CDS             complement(1432..1590)
                     /locus_tag="HVY57_RS24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(2260..2451)
                     /locus_tag="HVY57_RS24450"
                     /old_locus_tag="HVY57_24400"
     CDS             complement(2260..2451)
                     /locus_tag="HVY57_RS24450"
                     /old_locus_tag="HVY57_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2516..2839
                     /locus_tag="HVY57_RS24455"
                     /old_locus_tag="HVY57_24405"
     CDS             2516..2839
                     /locus_tag="HVY57_RS24455"
                     /old_locus_tag="HVY57_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3048..>3479
                     /locus_tag="HVY57_RS24860"
                     /pseudo=""
     CDS             3048..>3479
                     /locus_tag="HVY57_RS24860"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3517..4035
                     /locus_tag="HVY57_RS24465"
                     /old_locus_tag="HVY57_24415"
     CDS             3517..4035
                     /locus_tag="HVY57_RS24465"
                     /old_locus_tag="HVY57_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4042..4296
                     /locus_tag="HVY57_RS24470"
                     /old_locus_tag="HVY57_24420"
     CDS             4042..4296
                     /locus_tag="HVY57_RS24470"
                     /old_locus_tag="HVY57_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4614..5033)
                     /locus_tag="HVY57_RS24475"
                     /old_locus_tag="HVY57_24425"
     CDS             complement(4614..5033)
                     /locus_tag="HVY57_RS24475"
                     /old_locus_tag="HVY57_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5114..5551)
                     /locus_tag="HVY57_RS24480"
                     /old_locus_tag="HVY57_24430"
     CDS             complement(5114..5551)
                     /locus_tag="HVY57_RS24480"
                     /old_locus_tag="HVY57_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          2088..2175
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1519..2074)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2830..4400
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacaaaaaca ggtaacagca tcagaaacac gtattaaccg gataaatgct gatataactc
       61 agatccagaa ggctatttct caggtcagta ataatcgtaa tgccggtatc gctcgtgttc
      121 atgaagctga agaaaatttg aaaaaagcac agaataatct ccttaattca cagattaagg
      181 atgctgttga tgcaacagtt agcttttatc aaacgctgac tgaaaaatat ggtgaaaaat
      241 attcgaaaat ggcacaggaa cttgctgata agtctaaagg taagaaaatc ggcaatgtga
      301 atgaagctct cgctgctttt gaaaaataca aggatgtttt aaataagaaa ttcagcaaag
      361 ccgatcgtga tgctattttt aatgcgttgg catcggtgaa gtatgatgac tgggctaaac
      421 atttagatca gtttgccaag tacttgaaga ttacggggca tgtttctttt ggatatgatg
      481 tggtatctga tatcctaaaa attaaggata caggtgactg gaagccacta tttcttacat
      541 tagagaagaa agctgcagat gcaggggtga gttatgttgt tgctttactt tttagcttgc
      601 ttgctggaac tacattaggt atttggggta ttgctattgt tacaggaatt ctatgctcct
      661 atattgataa gaataaactt aatactataa atgaggtgtt agggatttaa ttattcttta
      721 ttgatataaa aagtcctagc aatccaaatg ggattgctag gaccaaacaa agtagattat
      781 atagcataaa taggtttaat tttgctacgg gggcgttatt taggtttttt cttctttcga
      841 aaaaatcttt ctttatgaag ttaaaagcta tgtattcaat agcatatttt gaatatggac
      901 atagaatagt gcttatcact attgcatata gcatcttatc tgacacaagg aaataatacc
      961 cttcgctgtt ttttgttata aggtatatat atataagtgt gcagtacagg ccaaataaaa
     1021 tattttttat gtagtatctt aagctcataa attaaacctc gccatatatt cttttcattt
     1081 tataaggatc gagttatgag gaaaagattt tttgtgggaa tattcgcgat aaacctcctt
     1141 gttggatgtc aggctaacta tatacgtgat gttcagggag ggaccatcgc accatcctcc
     1201 tcttctaaac tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc
     1261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccaacggtgg
     1321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1381 cgcagatacc aaatactgtc cttctagtgt agccgtagtc gggccactac ttcaagaact
     1441 ctgtagcacc gtttgtgcca tcatcgctct gctaatccgg ttaccagtgg ctgctgccag
     1501 tggcgttaag gcgtgcctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1621 cgaactgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1801 tctatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct gctacgtggc
     1861 cttcttcctg ttcttggtct tttgctcaca tgttctttcc ggccttatcc cctgattctg
     1921 tggataactg tgttaccgtt tttgtgtgag tcagtaccgc tcgccgcagt cgaacgaccg
     1981 agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct ggacgtgcat tttctcctta
     2041 cgcatctgtg cggcatttca cacccggcat ggcgtacttt tcatacaatc cgcactgatg
     2101 ccgcatggtt aagccagtat acactccgct atcgctacgt gactgggtca gggctgcgcc
     2161 ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2221 tcacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2281 cccgaaacgt gcgaggcagc tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca
     2341 gatgtctgcc tgttcatctg cgtccagttc gttgagcttc tccagcagcg ttaatgtctg
     2401 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttagtca ctgatgcctc
     2461 cgtgtaaggg ggtatttctg ttcatggggt aatgataccg atgaaacgcg agaggatgct
     2521 cacgatacgg gttactgatg atgaacatgc caggttactt gaacgctgtg agggtaaaca
     2581 actggcagta tggatgcggc gggtctgcct gggtgagccg gttgcccgtt ccgggaagct
     2641 gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcgggaata acctgaatca
     2701 gacagcgagg aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc
     2761 cgcactgatg gccatcgggg atgagctgcg ccggctgcgt ctggccgtca gggaacaggg
     2821 ggcgcgggat gatagttaaa tttcatgcca ggggaaaagg tggtggcagt ggtccggttg
     2881 attacctgct ggggagggag cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg
     2941 aagaagtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc
     3001 tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg
     3061 agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag catcctgtgg gtggagcacc
     3121 aggacaaggg acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa
     3181 aacgcctcca gccgtactac gaccgcgcag acaggcctag aattgatgcc tggcagacgc
     3241 tggtgaatca ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc aggacgctga
     3301 cactccctga taacctgcct gaaacgaaac aggcgcttgc tgagggcgtc acgcgaggta
     3361 tagatgcact ttaccatgcc ggagagataa aaggccgtca ggatgtgatt caggcgctca
     3421 ctgaggcggg gctggaagtg gtcagggtga cgcgaagcag tatcagcatt gcagatccga
     3481 acggcgggaa gaatatcagg ctgaaaggag cattttatga gcaatctttt gcagacgggc
     3541 gcggagttcg agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacaac
     3601 gagttcagga ggctcggcga atctgtaagc gaggctgtga catcaaacga gacgaaaatc
     3661 agagacgcta tagccctgtt cacagcctcg acagaggaat cgctggaaaa acaccgggaa
     3721 ggggtgaaag aggcgatgat gcagcacagg agggacgtgt taaagctggc agggaatacg
     3781 ggcatgatgt tactggggat agtctttctc ctgtttaccg cgagtggcgg gacgctctgg
     3841 tatcttggag ggaggataca ggcgaacctg gaagaaatca ggaagcagga agagacattg
     3901 cagaaactga acgcgaagac atggggcgtg gagtttgtgc aggacgggaa caggaaattc
     3961 cttgtccttc cgtacgggaa atcagcggag gtgattccct ttcaggggaa agagtgggta
     4021 catctgaagg agtgacacag agtgacagag ctggaaacac atttgctgaa cgccttagag
     4081 cagctgcaac aggactatat gcagcggctg agcgaatggg agagcgcctt cgtggaattg
     4141 cagaagatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag
     4201 ttgagtcagc aggtgcagca cttgagcgag cagacagaac gcttgagcga gcagacagaa
     4261 cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc
     4321 gcgaacatgt gttatccctg gaacgggagc gtcagccgga aatacaggaa cgcacgctgg
     4381 atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg
     4441 ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc
     4501 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4561 gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt
     4621 ctgccctcgt agccatcgag ataaaccgca gaaatcgtgt cagccagcag cctggatttt
     4681 ttcggggtaa ggtctttgct gcggtaatca gtacccgcaa attgagttgt gccccggagt
     4741 gagtttaaaa attcctggct gtgaaactgc tgaatgcgtt gttgcacatc actccgtgat
     4801 tttcctgtcg ctttatcttt agctcccata tcccagaact gccgaaaaaa aggcaccatc
     4861 attcgtgttg tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc atcagaagca
     4921 tatacgtaat gccgggtatg ttgttccacg ctgccctttg gggtacaggc tgtgagagct
     4981 actgttgctg ttatcatggc gataagacaa agtttgtttc cggtacgtct catgagtgtc
     5041 tccctacctt aaagtattta ttctcggtga gttattatcc gaagactatt ttatcatcag
     5101 tctctgacag cgttcagcga ccgaacaacc tggaccagaa tccccgtttc ttttccagtt
     5161 ccagtgcctg cctgagctgg gctatctcat tttgtagctg ttcccgttct gcttcctggc
     5221 gtctgcgatc catatcctgt gcctgtttat cctcaagcat cagcgtcagg cattgtttca
     5281 gctcattcag ctcccggaga atgcgttctg tctgctgatc atgtggattt tctgcatgtc
     5341 cctcactgtg cctttctggt gtctcattct gctttaattc gccgtatgcc cggatcagtt
     5401 cactggtttc aaactcccgt cgtccgtccc tgccggtacg gtaactgaca aggcctgcgc
     5461 gcatatcacg gtaaaactgt gaacgcgatc tgcctgtcat ttttagtgcg tcccggagtg
     5521 tgtgccatgc cataaagtga cagtgtccca tagatgtctc atctcatagt ttcagtaaaa
     5581 cataatgagg tctgagaacg gtaatgtttg tgctggtttt tgtggcatcg ggcgagaata
     5641 gcgcgtggtg tgaaagactg tttttttgat cgttttcaca aaaatggaag tccacagtct
     5701 tgacagggaa aatgcagcgg cgtagctttt atgctgtata taaaaccagt ggttatatgt
     5761 acagtattta tttttaactt attgttttaa aagtcaaaga ggattttata atggaaaccg
     5821 cggtagcgta ctataaagat ggtgttcctt atgatgataa gggacaggta attattactc
     5881 ttttgaatgg tactcctgac gggagtggct ctggcggcgg aggtggaaaa ggaggcagta
     5941 aaagtgaaag ttctgcagct attcatgcaa ctgctaaatg gtctactgct caattaaaga
     6001 aaacacaggc agagcaggct gcccgggcaa aagctgcagc ggaagcacag gcgaaagcaa
     6061 aggcaaacag ggatgcgctg actcagcgcc tgaaggatat cgtgaatgag gctcttcgtc
     6121 acaatgcctc acgtacgcct tcagcaacag agcttgctca tgctaataat gcagctatgc
     6181 aggcggaagc agagcgtttg cgccttgcga aagcagaaga aaaagcccgt aaagaagcgg
     6241 aagcagcaga aaaggctttt caggaagcag aacaacgacg taaagagatt gaacgggaga
     6301 aggctgaaac agaacgccag ttgaaactgg ctgaagctga agagaaacga ctggctgcat
     6361 tgagtgaaga agctaaagct gttgagatcg cccaaaaaaa actttctgct gcacaatctg
     6421 aagtggtgaa aatggatgga gagattaaga ctctcaattc tcgtttaagc tccagtatcc
     6481 atgcccgtga tgcagaaatg aaaacgctcg ctggaaaacg aaatgaactg gctcaggcat
     6541 ccgctaaata taaagaactg gatgagctgg tcaaaaaact atcaccaaga gccaatgatc
     6601 cgcttcagaa ccgtcctttt tttgaagcaa ccagacgacg ggttggggcc ggtaagatta
     6661 gagaagaaa
//
