LOCUS       NZ_CP057106             6669 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Escherichia fergusonii strain RHB38-C01 plasmid pRHB38-C01_3,
            complete sequence.
ACCESSION   NZ_CP057106
VERSION     NZ_CP057106.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148383
            Assembly: GCF_013812875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T3-C01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145.024101274844x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013812875.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 05:12:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,733
            CDSs (total)                      :: 4,615
            Genes (coding)                    :: 4,527
            CDSs (with protein)               :: 4,527
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 22 of 88
            Pseudo Genes (incomplete)         :: 57 of 88
            Pseudo Genes (internal stop)      :: 22 of 88
            Pseudo Genes (multiple problems)  :: 11 of 88
            Pseudo Genes (short protein)      :: 2 of 88
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP057106.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB38-C01"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB38-C01_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6484..6669,1..201))
                     /locus_tag="HVZ66_RS23440"
                     /old_locus_tag="HVZ66_23440"
     CDS             complement(join(6484..6669,1..201))
                     /locus_tag="HVZ66_RS23440"
                     /old_locus_tag="HVZ66_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000147848.1"
                     /translation="MTGRSRSQFYRDMRAGLVSYRTGRDGRREFETSELIRAYGELKQN
                     ETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQAQDMDRRRQEAEREQLQN
                     EIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            512..2080
                     /gene="cea"
                     /locus_tag="HVZ66_RS23445"
                     /old_locus_tag="HVZ66_23445"
     CDS             512..2080
                     /gene="cea"
                     /locus_tag="HVZ66_RS23445"
                     /old_locus_tag="HVZ66_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_181591625.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQMLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(2077..2418)
                     /locus_tag="HVZ66_RS23450"
                     /old_locus_tag="HVZ66_23450"
     CDS             complement(2077..2418)
                     /locus_tag="HVZ66_RS23450"
                     /old_locus_tag="HVZ66_23450"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            2466..2603
                     /locus_tag="HVZ66_RS23455"
                     /old_locus_tag="HVZ66_23455"
     CDS             2466..2603
                     /locus_tag="HVZ66_RS23455"
                     /old_locus_tag="HVZ66_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(2802..2960)
                     /locus_tag="HVZ66_RS23855"
     CDS             complement(2802..2960)
                     /locus_tag="HVZ66_RS23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(3630..3821)
                     /locus_tag="HVZ66_RS23460"
                     /old_locus_tag="HVZ66_23460"
     CDS             complement(3630..3821)
                     /locus_tag="HVZ66_RS23460"
                     /old_locus_tag="HVZ66_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3886..4209
                     /locus_tag="HVZ66_RS23465"
                     /old_locus_tag="HVZ66_23465"
     CDS             3886..4209
                     /locus_tag="HVZ66_RS23465"
                     /old_locus_tag="HVZ66_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4418..>4849
                     /locus_tag="HVZ66_RS23770"
                     /pseudo=""
     CDS             4418..>4849
                     /locus_tag="HVZ66_RS23770"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4887..5405
                     /locus_tag="HVZ66_RS23475"
                     /old_locus_tag="HVZ66_23475"
     CDS             4887..5405
                     /locus_tag="HVZ66_RS23475"
                     /old_locus_tag="HVZ66_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            5412..5666
                     /locus_tag="HVZ66_RS23480"
                     /old_locus_tag="HVZ66_23480"
     CDS             5412..5666
                     /locus_tag="HVZ66_RS23480"
                     /old_locus_tag="HVZ66_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5984..6403)
                     /locus_tag="HVZ66_RS23485"
                     /old_locus_tag="HVZ66_23485"
     CDS             complement(5984..6403)
                     /locus_tag="HVZ66_RS23485"
                     /old_locus_tag="HVZ66_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          3458..3545
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2889..3444)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4200..5770
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgcgttct gtctgctgat catgtggatt ttctgcatgt ccctcactgt gcctttctgg
       61 tgtctcattc tgctttaatt cgccgtatgc ccggatcagt tcactggttt caaactcccg
      121 tcgtccgtcc ctgccggtac ggtaactgac aaggcctgcg cgcatatcac ggtaaaactg
      181 tgaacgcgat ctgcctgtca tttttagtgc gtcccggagt gtgtgccatg ccataaagtg
      241 acagtgtccc atagatgtct catctcatag tttcagtaaa acataatgag gtctgagaac
      301 ggtaatgttt gtgctggttt ttgtggcatc gggcgagaat agcgcgtggt gtgaaagact
      361 gtttttttga tcgttttcac aaaaatggaa gtccacagtc ttgacaggga aaatgcagcg
      421 gcgtagcttt tatgctgtat ataaaaccag tggttatatg tacagtattt atttttaact
      481 tattgtttta aaagtcaaag aggattttat aatggaaacc gcggtagcgt actataaaga
      541 tggtgttcct tatgatgata agggacaggt aattattact cttttgaatg gtactcctga
      601 cgggagtggc tctggcggcg gaggtggaaa aggaggcagt aaaagtgaaa gttctgcagc
      661 tattcatgca actgctaaat ggtctactgc tcaattaaag aaaacacagg cagagcaggc
      721 tgcccgggca aaagctgcag cggaagcaca ggcgaaagca aaggcaaaca gggatgcgct
      781 gactcagcgc ctgaaggata tcgtgaatga ggctcttcgt cacaatgcct cacgtacgcc
      841 ttcagcaaca gagcttgctc atgctaataa tgcagctatg caggcggaag cagagcgttt
      901 gcgccttgcg aaagcagaag aaaaagcccg taaagaagcg gaagcagcag aaaaggcttt
      961 tcaggaagca gaacaacgac gtaaagagat tgaacgggag aaggctgaaa cagaacgcca
     1021 gttgaaactg gctgaagctg aagagaaacg actggctgca ttgagtgaag aagctaaagc
     1081 tgttgagatc gcccaaaaaa aactttctgc tgcacaatct gaagtggtga aaatggatgg
     1141 agagattaag actctcaatt ctcgtttaag ctccagtatc catgcccgtg atgcagaaat
     1201 gaaaacgctc gctggaaaac gaaatgaact ggctcaggca tccgctaaat ataaagaact
     1261 ggatgagctg gtcaaaaaac tatcaccaag agccaatgat ccgcttcaga accgtccttt
     1321 ttttgaagca accagacgac gggttggggc cggtaagatt agagaagaaa aacaaaaaca
     1381 ggtaacagca tcagaaacac gtattaaccg gataaatgct gatataactc agatccagaa
     1441 ggctatttct caggtcagta ataatcgtaa tgccggtatc gctcgtgttc atgaagctga
     1501 agaaaatttg aaaaaagcac agaataatct ccttaattca cagattaagg atgctgttga
     1561 tgcaacagtt agcttttatc aaatgctgac tgaaaaatat ggtgaaaaat attcgaaaat
     1621 ggcacaggaa cttgctgata agtctaaagg taagaaaatc ggcaatgtga atgaagctct
     1681 cgctgctttt gaaaaataca aggatgtttt aaataagaaa ttcagcaaag ccgatcgtga
     1741 tgctattttt aatgcgttgg catcggtgaa gtatgatgac tgggctaaac atttagatca
     1801 gtttgccaag tacttgaaga ttacggggca tgtttctttt ggatatgatg tggtatctga
     1861 tatcctaaaa attaaggata caggtgactg gaagccacta tttcttacat tagagaagaa
     1921 agctgcagat gcaggagtga gttatgttgt tgctttactt tttagcttgc ttgctggaac
     1981 tacattaggt atttggggta ttgctattgt tacaggaatt ctatgctcct atattgataa
     2041 gaataaactt aatactataa atgaggtgtt agggatttaa ttattcttta ttgatataaa
     2101 aagtcctagc aatccaaatg ggattgctag gaccaaacaa agtagattat atagcataaa
     2161 taggtttaat tttgctacgg gggcgttatt taggtttttt cttctttcga aaaaatcttt
     2221 ctttatgaag ttaaaagcta tgtattcaat agcatatttt gaatatggac atagaatagt
     2281 gcttatcact attgcatata gcatcttatc tgacacaagg aaataatacc cttcgctgtt
     2341 ttttgttata aggtatatat atataagtgt gcagtacagg ccaaataaaa tattttttat
     2401 gtagtatctt aagctcataa attaaacctc gccatatatt cttttcattt tataaggatc
     2461 gagttatgag gaaaagattt tttgtgggaa tattcgcgat aaacctcctt gttggatgtc
     2521 aggctaacta tatacgtgat gttcagggag ggaccatcgc accatcctcc tcttctaaac
     2581 tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct
     2641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccaacggtgg tttgtttgcc
     2701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2761 aaatactgtc cttctagtgt agccgtagtc gggccactac ttcaagaact ctgtagcacc
     2821 gtttgtgcca tcatcgctct gctaatccgg ttaccagtgg ctgctgccag tggcgttaag
     2881 gcgtgcctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2941 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3001 taccaacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3061 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tctatttttg
     3181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcct gctacgtggc cttcttcctg
     3241 ttcttggtct tttgctcaca tgttctttcc ggccttatcc cctgattctg tggataactg
     3301 tgttaccgtt tttgtgtgag tcagtaccgc tcgccgcagt cgaacgaccg agcgtagcga
     3361 gtcagtgagc gaggaagcgg aaaagcgcct ggacgtgcat tttctcctta cgcatctgtg
     3421 cggcatttca cacccggcat ggcgtacttt tcatacaatc cgcactgatg ccgcatggtt
     3481 aagccagtat acactccgct atcgctacgt gactgggtca gggctgcgcc ccgacacccg
     3541 ctaaaacctg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc tcacagacaa
     3601 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc cccgaaacgt
     3661 gcgaggcagc tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca gatgtctgcc
     3721 tgttcatctg cgtccagttc gttgagcttc tccagcagcg ttaatgtctg gcttctgata
     3781 aagcgggcca tgttaagggc ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg
     3841 ggtatttctg ttcatggggt aatgataccg atgaaacgcg agaggatgct cacgatacgg
     3901 gttactgatg atgaacatgc caggttactt gaacgctgtg agggtaaaca actggcagta
     3961 tggatgcggc gggtctgcct gggtgagccg gttgcccgtt ccgggaagct gccgacactg
     4021 gcaccgccgt tactgcgtca gctggccgcc atcgggaata acctgaatca gacagcgagg
     4081 aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc cgcactgatg
     4141 gccatcgggg atgagctgcg ccggctgcgt ctggccgtca gggaacaggg ggcgcgggat
     4201 gatagttaaa tttcatgcca ggggaaaagg tggtggcagt ggtccggttg attacctgct
     4261 ggggagggag cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg aagaagtccg
     4321 ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc tgtcgttcgc
     4381 ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg agcgtgtact
     4441 gatgcccggt ctcgaaaaaa atcagtacag catcctgtgg gtggagcacc aggacaaggg
     4501 acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca
     4561 gccgtactac gaccgcgcag acaggcctag aattgatgcc tggcagacgc tggtgaatca
     4621 ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc aggacgctga cactccctga
     4681 taacctgcct gaaacgaaac aggcgcttgc tgagggcgtc acgcgaggta tagatgcact
     4741 ttaccatgcc ggagagataa aaggccgtca ggatgtgatt caggcgctca ctgaggcggg
     4801 gctggaagtg gtcagggtga cgcgaagcag tatcagcatt gcagatccga acggcgggaa
     4861 gaatatcagg ctgaaaggag cattttatga gcaatctttt gcagacgggc gcggagttcg
     4921 agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacaac gagttcagga
     4981 ggctcggcga atctgtaagc gaggctgtga catcaaacga gacgaaaatc agagacgcta
     5041 tagccctgtt cacagcctcg acagaggaat cgctggaaaa acaccgggaa ggggtgaaag
     5101 aggcgatgat gcagcacagg agggacgtgt taaagctggc agggaatacg ggcatgatgt
     5161 tactggggat agtctttctc ctgtttaccg cgagtggcgg gacgctctgg tatcttggag
     5221 ggaggataca ggcgaacctg gaagaaatca ggaagcagga agagacattg cagaaactga
     5281 acgcgaagac atggggcgtg gagtttgtgc aggacgggaa caggaaattc cttgtccttc
     5341 cgtacgggaa atcagcggag gtgattccct ttcaggggaa agagtgggta catctgaagg
     5401 agtgacacag agtgacagag ctggaaacac atttgctgaa cgccttagag cagctgcaac
     5461 aggactatat gcagcggctg agcgaatggg agagcgcctt cgtggaattg cagaagatgt
     5521 tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc
     5581 aggtgcagca cttgagcgag cagacagaac gcttgagcga gcagacagaa cgcttgagcc
     5641 agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt
     5701 gttatccctg gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc
     5761 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     5821 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgg
     5881 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg
     5941 cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt
     6001 agccatcgag ataaaccgca gaaatcgtgt cagccagcag cctggatttt ttcggggtaa
     6061 ggtctttgct gcggtaatca gtacccgcaa attgagttgt gccccggagt gagtttaaaa
     6121 attcctggct gtgaaactgc tgaatgcgtt gttgcacatc actccgtgat tttcctgtcg
     6181 ctttatcttt agctcccata tcccagaact gccgaaaaaa aggcaccatc attcgtgttg
     6241 tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc atcagaagca tatacgtaat
     6301 gccgggtatg ttgttccacg ctgccctttg gggtacaggc tgtgagagct actgttgctg
     6361 ttatcatggc gataagacaa agtttgtttc cggtacgtct catgagtgtc tccctacctt
     6421 aaagtattta ttctcggtga gttattatcc gaagactatt ttatcatcag tctctgacag
     6481 cgttcagcga ccgaacaacc tggaccagaa tccccgtttc ttttccagtt ccagtgcctg
     6541 cctgagctgg gctatctcat tttgtagctg ttcccgttct gcttcctggc gtctgcgatc
     6601 catatcctgt gcctgtttat cctcaagcat cagcgtcagg cattgtttca gctcattcag
     6661 ctcccggag
//
