LOCUS       NZ_CP057102             6647 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Escherichia fergusonii strain RHB38-C04 plasmid pRHB38-C04_2,
            complete sequence.
ACCESSION   NZ_CP057102
VERSION     NZ_CP057102.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148384
            Assembly: GCF_013813425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T3-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 155.03934376227x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013813425.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 04:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,345
            Genes (coding)                    :: 4,254
            CDSs (with protein)               :: 4,254
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 33 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 17 of 91
            Pseudo Genes (multiple problems)  :: 8 of 91
            Pseudo Genes (short protein)      :: 2 of 91
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP057102.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB38-C04"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB38-C04_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6245..6647,1..17)
                     /locus_tag="HVZ67_RS22090"
                     /old_locus_tag="HVZ67_22100"
     CDS             join(6245..6647,1..17)
                     /locus_tag="HVZ67_RS22090"
                     /old_locus_tag="HVZ67_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(335..568)
                     /locus_tag="HVZ67_RS22095"
                     /old_locus_tag="HVZ67_22105"
     CDS             complement(335..568)
                     /locus_tag="HVZ67_RS22095"
                     /old_locus_tag="HVZ67_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(575..1093)
                     /locus_tag="HVZ67_RS22100"
                     /old_locus_tag="HVZ67_22110"
     CDS             complement(575..1093)
                     /locus_tag="HVZ67_RS22100"
                     /old_locus_tag="HVZ67_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1131..1829)
                     /locus_tag="HVZ67_RS22535"
                     /old_locus_tag="HVZ67_22115"
                     /pseudo=""
     CDS             complement(<1131..1829)
                     /locus_tag="HVZ67_RS22535"
                     /old_locus_tag="HVZ67_22115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1771..2094)
                     /locus_tag="HVZ67_RS22110"
                     /old_locus_tag="HVZ67_22120"
     CDS             complement(1771..2094)
                     /locus_tag="HVZ67_RS22110"
                     /old_locus_tag="HVZ67_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2158..2349
                     /locus_tag="HVZ67_RS22115"
                     /old_locus_tag="HVZ67_22125"
     CDS             2158..2349
                     /locus_tag="HVZ67_RS22115"
                     /old_locus_tag="HVZ67_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3019..3177
                     /locus_tag="HVZ67_RS22540"
     CDS             3019..3177
                     /locus_tag="HVZ67_RS22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            3675..3902
                     /locus_tag="HVZ67_RS22125"
                     /old_locus_tag="HVZ67_22135"
     CDS             3675..3902
                     /locus_tag="HVZ67_RS22125"
                     /old_locus_tag="HVZ67_22135"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(3899..5467)
                     /gene="cea"
                     /locus_tag="HVZ67_RS22130"
                     /old_locus_tag="HVZ67_22140"
     CDS             complement(3899..5467)
                     /gene="cea"
                     /locus_tag="HVZ67_RS22130"
                     /old_locus_tag="HVZ67_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            5727..6164
                     /locus_tag="HVZ67_RS22135"
                     /old_locus_tag="HVZ67_22145"
     CDS             5727..6164
                     /locus_tag="HVZ67_RS22135"
                     /old_locus_tag="HVZ67_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          complement(2435..2522)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2533..3091
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(232..1781)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgagggcag acagtaagtg gatttaccat aatcccttaa ttgtacgcac cgctaaaacg
       61 cgttcagcgc gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg
      121 ccgcgatcgc gcctgataaa ttttaaccgt atgaatacct atgcaaccag agggtacagg
      181 ccacattacc cccacttaat ccactgaagc tgccattttt catggtttca ccatcccagc
      241 gaagggccat ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc cagggataac
      301 acatgttcgc gctcctgtat cagccgttcc tctcttatct ccagttctcg ctgtataact
      361 ggctcaagcg ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc
      421 gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa catcttctgc aattccacga
      481 aggcgctctc ccattcgctc agccgctgca tatagtcctg ttgcagctgc tctaaggcgt
      541 tcagcaaatg tgtttccagc tctgtcactc tgtgtcactc cttcagatgt acccactctt
      601 tcccctgaaa gggaatcacc tccgctgatt tcccgtacgg aaggacaagg aatttcctgt
      661 tcccgtcctg cacaaactcc acgccccatg tcttcgcgtt cagtttctgc aatgtctctt
      721 cctgcttcct gatttcttcc aggttcgcct gtatcctccc tccaagatac cagagcgtcc
      781 cgccactcgc ggtaaacagg agaaagacta tccccagtaa catcatgccc gtattccctg
      841 ccagctttaa cacgtccctc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt
      901 tttccagcga ttcctctgtc gaggctgtga acagggctat agcgtctctg attttcgtct
      961 cgtttgatgt cacagcctcg cttacagatt cgccgagcct cctgaactcg ttgttcagca
     1021 ttttctctgt agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgca
     1081 aaagattgct cataaaatgc tcctttcagc ctgatattct tcccgccgtt cggatctgca
     1141 atgctgatac tgcttcgcgt caccctgacc acttccagcc ccgcctcagt gagcgcctga
     1201 atcacatcct gacggccttt tatctctccg gcatggtaaa gtgcatctat acctcgcgtg
     1261 acgccctcag caagcgcctg tttcgtttca ggcaggttat cagggagtgt cagcgtcctg
     1321 cggttctccg gggcgttcgg gtcatgcagc ccgtaatggt gattcaccag cgtctgccag
     1381 gcatcaattc taggcctgtc tgcgcggtcg tagtacggct ggaggcgttt tccggtctgt
     1441 agctccatgt tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc
     1501 cacaggatgc tgtactgatt tttttcgaga ccgggcatca gtacacgctc aaagctcgcc
     1561 atcacttttt cacgtcctcc cggcggcagc tccttctccg cgaacgacag aacaccggac
     1621 gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc ggacttcttc cggattaccc
     1681 tgaagcaccg ttgcgccttc gcggttacgc tccctcccca gcaggtaatc aaccggacca
     1741 ctgccaccac cttttcccct ggcatgaaat ttaactatca tcccgcgccc cctgttccct
     1801 gacagccaga cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg
     1861 aacccggtca ccggaagacc actgcccgct gttcacctta cgggctgtct gattcaggtt
     1921 atttccgatg gcggccagct gacgcagtaa cggcggtgcc agtgtcggca gttttccgga
     1981 acgggcaacc ggctccccca ggcagacccg ccgcatccat accgccagtt gtttaccctc
     2041 acagcgttca agtaaccggg catgttcatc atcagtaacc cgtattgtga gcatcctctc
     2101 gcgtttcatc ggtatcatta ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     2161 actaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     2221 ctgctggaga agctcaacga actggacgca gatgaacagg ccgatatttg tgaatcgctt
     2281 cacgaccacg ccgatgagct ttaccgcagc tgcctcgcac gtttcgggga tgacggtgaa
     2341 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtgagc ggatgccggg
     2401 agctgacaag cccgtcaggg cgcgtcagca ggttttagcg ggtgtcgggg cgcagccctg
     2461 acccagtcac gtagcgatag cggagtgtat actggcttaa ccatgcggca tcagtgcgga
     2521 ttgtatgaaa agtacgccat gccgggtgtg aaatgccgca cagatgcgta aggagaaaat
     2581 gcacgtccag gcgcttttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     2641 tgcggcgagc ggtactgact cacacaaaaa cggtaacaca gttatccaca gaatcagggg
     2701 ataaggccgg aaagaacatg tgagcaaaag accaggaaca ggaagaaggc cacgtagcag
     2761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatagacg ctcaagtcag
     2821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2941 ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt
     3001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3061 ggtaactatc gtcttgagtc caacccggta aggcacgcct taacgccact ggcagcagcc
     3121 actggtaacc ggattagcag agcgatgatg gcacaaacgg tgctacagag ttcttgaagt
     3181 agtggcccga ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3241 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgttggta
     3301 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3361 atcctttgat cttttctact gaaccgcgat ccccgtcagt ttagaagagg aggatggtgc
     3421 gatggtccct ccctgaacat cacgtatata gttagcctga catccaacaa ggaggtttat
     3481 cgcgaatatt cccacaaaaa atcttttcct cataactcga tccttataaa atgaaaagaa
     3541 tatatggcga ggtttaattt atgagcttaa gatactacat aaaaaatatt ttatttggcc
     3601 tgtactgcac acttatatat atatacctta taacaaaaaa cagcgaaggg tattatttcc
     3661 ttgtgtcaga taagatgcta tatgcaatag tgataagcac tattctatgt ccatattcaa
     3721 aatatgctat tgaatacata gcttttaact tcataaagaa agattttttc gaaagaagaa
     3781 aaaacctaaa taacgccccc gtagcaaaat taaacctatt tatgctatat aatctacttt
     3841 gtttggtcct agcaatccca tttggattgc taggactttt tatatcaata aagaataatt
     3901 aaatccctaa cacctcattt atagtattaa gtttattctt atcaatatag gagcatagaa
     3961 ttcctgtaac aatagcaata ccccaaatac ctaatgtagt tccagcaagc aagctaaaaa
     4021 gtaaagcaac aacataactc acccctgcat ctgcagcttt cttctctaat gtaagaaata
     4081 gtggcttcca gtcacctgta tccttaattt ttaggatatc agataccaca tcatatccaa
     4141 aagaaacatg ccccgtaatc ttcaagtact tggcaaactg atctaaatgt ttagcccagt
     4201 catcatactt caccgatgcc aacgcattaa aaatagcatc acgatcggct ttgctgaatt
     4261 tcttatttaa aacatccttg tatttttcaa aagcagcgag agcttcattc acattgccga
     4321 ttttcttacc tttagactta tcagcaagtt cctgtgccat tttcgaatat ttttcaccat
     4381 atttttcagt cagcgtttga taaaagctaa ctgttgcatc aacagcatcc ttaatctgtg
     4441 aattaaggag attattctgt gcttttttca aattttcttc agcttcatga acacgagcga
     4501 taccggcatt acgattatta ctgacctgag aaatagcctt ctggatctga gttatatcag
     4561 catttatccg gttaatacgt gtttctgatg ctgttacctg tttttgtttt tcttctctaa
     4621 tcttaccggc cccaacccgt cgtctggttg cttcaaaaaa aggacggttc tgaagcggat
     4681 cattggctct tggtgatagt tttttgacca gctcatccag ttctttatat ttagcggatg
     4741 cctgagccag ttcatttcgt tttccagcga gcgttttcat ttctgcatca cgggcatgga
     4801 tactggagct taaacgagaa ttgagagtct taatctctcc atccattttc accacttcag
     4861 attgtgcagc agaaagtttt ttttgggcga tctcaacagc tttagcttct tcactcaatg
     4921 cagccagtcg tttctcttca gcttcagcca gtttcaactg gcgttctgtt tcagccttct
     4981 cccgttcaat ctctttacgt cgttgttctg cttcctgaaa agccttttct gctgcttccg
     5041 cttctttacg ggctttttct tctgctttcg caaggcgcaa acgctctgct tccgcctgca
     5101 tagctgcatt attagcatga gcaagctctg ttgctgaagg cgtacgtgag gcattgtgac
     5161 gaagagcctc attcacgata tccttcaggc gctgagtcag cgcatccctg tttgcctttg
     5221 ctttcgcctg tgcttccgct gcagcttttg cccgggcagc ctgctctgcc tgtgttttct
     5281 ttaattgagc agtagaccat ttagcagttg catgaatagc tgcagaactt tcacttttac
     5341 tgcctccttt tccacctccg ccgccagagc cactcccgtc aggagtacca ttcaaaagag
     5401 taataattac ctgtccctta tcatcataag gaacaccatc tttatagtac gctaccgcgg
     5461 tttccattat aaaatcctct ttgactttta aaacaataag ttaaaaataa atactgtaca
     5521 tataaccact ggttttatat acagcataaa agctacgccg ctgcattttc cctgtcaaga
     5581 ctgtggactt ccatttttgt gaaaacgatc aaaaaaacag tctttcacac cacgcgctat
     5641 tctcgcccga tgccacaaaa accagcacaa acattaccgt tctcagacct cattatgttt
     5701 tactgaaact atgagatgag acatctatgg gacactgtca ctttatggca tggcacacac
     5761 tccgggacgc actaaaaatg acaggcagat cgcgttcaca gttttaccgt gatatgcgcg
     5821 caggccttgt cagttaccgt accggcaggg acggacgacg ggagtttgaa accagtgaac
     5881 tgatccgggc atacggcgaa ttaaagcaga atgagacacc agaaaggcac agtgagggac
     5941 atgcagaaaa tccacatgat cagcagacag aacgcattct ccgggagctg aatgagctga
     6001 aacaatgcct gacgctgatg cttgaggata aacaggcaca ggatatggat cgcagacgcc
     6061 aggaagcaga acgggaacag ctacaaaatg agatagccca gctcaggcag gcactggaac
     6121 tggaaaagaa acggggattc tggtccaggt tgttcggtcg ctgaacgctg tcagagactg
     6181 atgataaaat agtcttcgga taataactca ccgagaataa atactttaag gtagggagac
     6241 actcatgaga cgtaccggaa acaaactttg tcttatcgcc atgataacag caacagtagc
     6301 tctcacagcc tgtaccccaa agggcagcgt ggaacaacat acccggcatt acgtatatgc
     6361 ttctgatgac ggttttgatc ccaacttttc cacccaaaaa gccgacacaa cacgaatgat
     6421 ggtgcctttt tttcggcagt tctgggatat gggagctaaa gataaagcga caggaaaatc
     6481 acggagtgat gtgcaacaac gcattcagca gtttcacagc caggaatttt taaactcact
     6541 ccggggcaca actcaatttg cgggtactga ttaccgcagc aaagacctta ccccgaaaaa
     6601 atccaggctg ctggctgaca cgatttctgc ggtttatctc gatggct
//
