LOCUS       NZ_CP057095             6669 bp    DNA     circular CON 02-APR-2024
DEFINITION  Escherichia fergusonii strain RHB38-C07 plasmid pRHB38-C07_3,
            complete sequence.
ACCESSION   NZ_CP057095
VERSION     NZ_CP057095.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148386
            Assembly: GCF_013813205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T3-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.110310255315x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013813205.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 04:20:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,646
            Genes (coding)                    :: 4,502
            CDSs (with protein)               :: 4,502
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 52 of 144
            Pseudo Genes (incomplete)         :: 85 of 144
            Pseudo Genes (internal stop)      :: 27 of 144
            Pseudo Genes (multiple problems)  :: 21 of 144
            Pseudo Genes (short protein)      :: 2 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP057095.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB38-C07"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB38-C07_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5572..6669,1..477)
                     /locus_tag="HVZ69_RS23955"
     CDS             join(5572..6669,1..477)
                     /locus_tag="HVZ69_RS23955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_072032120.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNL
                     PETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKN
                     IRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRY
                     SPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSW
                     REDTGEPGRNQEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTS
                     EGVTQSDRAGNTFAERLRAAATGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAV
                     ESAGAALERADRTLERADRTLEPVIQRELEIREERLIQEREHVLSLERERQPEIQERTL
                     DGPSLGW"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(688..1107)
                     /locus_tag="HVZ69_RS23580"
                     /old_locus_tag="HVZ69_23570"
     CDS             complement(688..1107)
                     /locus_tag="HVZ69_RS23580"
                     /old_locus_tag="HVZ69_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1188..1625)
                     /locus_tag="HVZ69_RS23585"
                     /old_locus_tag="HVZ69_23575"
     CDS             complement(1188..1625)
                     /locus_tag="HVZ69_RS23585"
                     /old_locus_tag="HVZ69_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1885..3453
                     /gene="cea"
                     /locus_tag="HVZ69_RS23590"
                     /old_locus_tag="HVZ69_23580"
     CDS             1885..3453
                     /gene="cea"
                     /locus_tag="HVZ69_RS23590"
                     /old_locus_tag="HVZ69_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_181591625.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQMLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(3450..3791)
                     /locus_tag="HVZ69_RS23595"
                     /old_locus_tag="HVZ69_23585"
     CDS             complement(3450..3791)
                     /locus_tag="HVZ69_RS23595"
                     /old_locus_tag="HVZ69_23585"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3839..3976
                     /locus_tag="HVZ69_RS23600"
                     /old_locus_tag="HVZ69_23590"
     CDS             3839..3976
                     /locus_tag="HVZ69_RS23600"
                     /old_locus_tag="HVZ69_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(4175..4333)
                     /locus_tag="HVZ69_RS24070"
     CDS             complement(4175..4333)
                     /locus_tag="HVZ69_RS24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5003..5194)
                     /locus_tag="HVZ69_RS23605"
                     /old_locus_tag="HVZ69_23595"
     CDS             complement(5003..5194)
                     /locus_tag="HVZ69_RS23605"
                     /old_locus_tag="HVZ69_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5259..5582
                     /locus_tag="HVZ69_RS23610"
                     /old_locus_tag="HVZ69_23600"
     CDS             5259..5582
                     /locus_tag="HVZ69_RS23610"
                     /old_locus_tag="HVZ69_23600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4831..4918
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4262..4817)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..474
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.06e-69"
     Typing          5573..6669
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.989"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 cgtggagttt gtgcaggacg ggaacaggaa attccttgtc cttccgtacg ggaaatcagc
       61 ggaggtgatt ccctttcagg ggaaagagtg ggtacatctg aaggagtgac acagagtgac
      121 agagctggaa acacatttgc tgaacgcctt agagcagctg caacaggact atatgcagcg
      181 gctgagcgaa tgggagagcg ccttcgtgga attgcagaag atgttttcgc ttacgcaacg
      241 ggacaacgcg atgctgaacg agcgggtcat gcagttgagt cagcaggtgc agcacttgag
      301 cgagcagaca gaacgcttga gcgagcagac agaacgcttg agccagttat acagcgagaa
      361 ctggagataa gagaggaacg gctgatacag gagcgcgaac atgtgttatc cctggaacgg
      421 gagcgtcagc cggaaataca ggaacgcacg ctggatggcc cttcgctggg atggtgaaac
      481 catgaaaaat ggcagcttca gtggattaag tgggggtaat gtggcctgta ccctctggtt
      541 gcataggtat tcatacggtt aaaatttatc aggcgcgatc gcggcagttt ttcgggtggt
      601 ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt
      661 acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac
      721 cgcagaaatc gtgtcagcca gcagcctgga ttttttcggg gtaaggtctt tgctgcggta
      781 atcagtaccc gcaaattgag ttgtgccccg gagtgagttt aaaaattcct ggctgtgaaa
      841 ctgctgaatg cgttgttgca catcactccg tgattttcct gtcgctttat ctttagctcc
      901 catatcccag aactgccgaa aaaaaggcac catcattcgt gttgtgtcgg ctttttgggt
      961 ggaaaagttg ggatcaaaac cgtcatcaga agcatatacg taatgccggg tatgttgttc
     1021 cacgctgccc tttggggtac aggctgtgag agctactgtt gctgttatca tggcgataag
     1081 acaaagtttg tttccggtac gtctcatgag tgtctcccta ccttaaagta tttattctcg
     1141 gtgagttatt atccgaagac tattttatca tcagtctctg acagcgttca gcgaccgaac
     1201 aacctggacc agaatccccg tttcttttcc agttccagtg cctgcctgag ctgggctatc
     1261 tcattttgta gctgttcccg ttctgcttcc tggcgtctgc gatccatatc ctgtgcctgt
     1321 ttatcctcaa gcatcagcgt caggcattgt ttcagctcat tcagctcccg gagaatgcgt
     1381 tctgtctgct gatcatgtgg attttctgca tgtccctcac tgtgcctttc tggtgtctca
     1441 ttctgcttta attcgccgta tgcccggatc agttcactgg tttcaaactc ccgtcgtccg
     1501 tccctgccgg tacggtaact gacaaggcct gcgcgcatat cacggtaaaa ctgtgaacgc
     1561 gatctgcctg tcatttttag tgcgtcccgg agtgtgtgcc atgccataaa gtgacagtgt
     1621 cccatagatg tctcatctca tagtttcagt aaaacataat gaggtctgag aacggtaatg
     1681 tttgtgctgg tttttgtggc atcgggcgag aatagcgcgt ggtgtgaaag actgtttttt
     1741 tgatcgtttt cacaaaaatg gaagtccaca gtcttgacag ggaaaatgca gcggcgtagc
     1801 ttttatgctg tatataaaac cagtggttat atgtacagta tttattttta acttattgtt
     1861 ttaaaagtca aagaggattt tataatggaa accgcggtag cgtactataa agatggtgtt
     1921 ccttatgatg ataagggaca ggtaattatt actcttttga atggtactcc tgacgggagt
     1981 ggctctggcg gcggaggtgg aaaaggaggc agtaaaagtg aaagttctgc agctattcat
     2041 gcaactgcta aatggtctac tgctcaatta aagaaaacac aggcagagca ggctgcccgg
     2101 gcaaaagctg cagcggaagc acaggcgaaa gcaaaggcaa acagggatgc gctgactcag
     2161 cgcctgaagg atatcgtgaa tgaggctctt cgtcacaatg cctcacgtac gccttcagca
     2221 acagagcttg ctcatgctaa taatgcagct atgcaggcgg aagcagagcg tttgcgcctt
     2281 gcgaaagcag aagaaaaagc ccgtaaagaa gcggaagcag cagaaaaggc ttttcaggaa
     2341 gcagaacaac gacgtaaaga gattgaacgg gagaaggctg aaacagaacg ccagttgaaa
     2401 ctggctgaag ctgaagagaa acgactggct gcattgagtg aagaagctaa agctgttgag
     2461 atcgcccaaa aaaaactttc tgctgcacaa tctgaagtgg tgaaaatgga tggagagatt
     2521 aagactctca attctcgttt aagctccagt atccatgccc gtgatgcaga aatgaaaacg
     2581 ctcgctggaa aacgaaatga actggctcag gcatccgcta aatataaaga actggatgag
     2641 ctggtcaaaa aactatcacc aagagccaat gatccgcttc agaaccgtcc tttttttgaa
     2701 gcaaccagac gacgggttgg ggccggtaag attagagaag aaaaacaaaa acaggtaaca
     2761 gcatcagaaa cacgtattaa ccggataaat gctgatataa ctcagatcca gaaggctatt
     2821 tctcaggtca gtaataatcg taatgccggt atcgctcgtg ttcatgaagc tgaagaaaat
     2881 ttgaaaaaag cacagaataa tctccttaat tcacagatta aggatgctgt tgatgcaaca
     2941 gttagctttt atcaaatgct gactgaaaaa tatggtgaaa aatattcgaa aatggcacag
     3001 gaacttgctg ataagtctaa aggtaagaaa atcggcaatg tgaatgaagc tctcgctgct
     3061 tttgaaaaat acaaggatgt tttaaataag aaattcagca aagccgatcg tgatgctatt
     3121 tttaatgcgt tggcatcggt gaagtatgat gactgggcta aacatttaga tcagtttgcc
     3181 aagtacttga agattacggg gcatgtttct tttggatatg atgtggtatc tgatatccta
     3241 aaaattaagg atacaggtga ctggaagcca ctatttctta cattagagaa gaaagctgca
     3301 gatgcaggag tgagttatgt tgttgcttta ctttttagct tgcttgctgg aactacatta
     3361 ggtatttggg gtattgctat tgttacagga attctatgct cctatattga taagaataaa
     3421 cttaatacta taaatgaggt gttagggatt taattattct ttattgatat aaaaagtcct
     3481 agcaatccaa atgggattgc taggaccaaa caaagtagat tatatagcat aaataggttt
     3541 aattttgcta cgggggcgtt atttaggttt tttcttcttt cgaaaaaatc tttctttatg
     3601 aagttaaaag ctatgtattc aatagcatat tttgaatatg gacatagaat agtgcttatc
     3661 actattgcat atagcatctt atctgacaca aggaaataat acccttcgct gttttttgtt
     3721 ataaggtata tatatataag tgtgcagtac aggccaaata aaatattttt tatgtagtat
     3781 cttaagctca taaattaaac ctcgccatat attcttttca ttttataagg atcgagttat
     3841 gaggaaaaga ttttttgtgg gaatattcgc gataaacctc cttgttggat gtcaggctaa
     3901 ctatatacgt gatgttcagg gagggaccat cgcaccatcc tcctcttcta aactgacggg
     3961 gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4021 atctgctgct tgcaaacaaa aaaaccaccg ctaccaacgg tggtttgttt gccggatcaa
     4081 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4141 gtccttctag tgtagccgta gtcgggccac tacttcaaga actctgtagc accgtttgtg
     4201 ccatcatcgc tctgctaatc cggttaccag tggctgctgc cagtggcgtt aaggcgtgcc
     4261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agataccaac
     4381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtctattt ttgtgatgct
     4561 cgtcaggggg gcggagccta tggaaaaacg cctgctacgt ggccttcttc ctgttcttgg
     4621 tcttttgctc acatgttctt tccggcctta tcccctgatt ctgtggataa ctgtgttacc
     4681 gtttttgtgt gagtcagtac cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg
     4741 agcgaggaag cggaaaagcg cctggacgtg cattttctcc ttacgcatct gtgcggcatt
     4801 tcacacccgg catggcgtac ttttcataca atccgcactg atgccgcatg gttaagccag
     4861 tatacactcc gctatcgcta cgtgactggg tcagggctgc gccccgacac ccgctaaaac
     4921 ctgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgctcacaga caagctgtga
     4981 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atccccgaaa cgtgcgaggc
     5041 agctgcggta aagctcatcg gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     5101 ctgcgtccag ttcgttgagc ttctccagca gcgttaatgt ctggcttctg ataaagcggg
     5161 ccatgttaag ggcggttttt tcctgtttag tcactgatgc ctccgtgtaa gggggtattt
     5221 ctgttcatgg ggtaatgata ccgatgaaac gcgagaggat gctcacgata cgggttactg
     5281 atgatgaaca tgccaggtta cttgaacgct gtgagggtaa acaactggca gtatggatgc
     5341 ggcgggtctg cctgggtgag ccggttgccc gttccgggaa gctgccgaca ctggcaccgc
     5401 cgttactgcg tcagctggcc gccatcggga ataacctgaa tcagacagcg aggaaggtga
     5461 acagcgggca gtggtcttcc ggtgaccggg ttcaggtggt ggccgcactg atggccatcg
     5521 gggatgagct gcgccggctg cgtctggccg tcagggaaca gggggcgcgg gatgatagtt
     5581 aaatttcatg ccaggggaaa aggtggtggc agtggtccgg ttgattacct gctggggagg
     5641 gagcgtaacc gcgaaggcgc aacggtgctt cagggtaatc cggaagaagt ccgggaactc
     5701 atcgatgcca cgccatttgc gaagaaatac acgtccggtg ttctgtcgtt cgcggagaag
     5761 gagctgccgc cgggaggacg tgaaaaagtg atggcgagct ttgagcgtgt actgatgccc
     5821 ggtctcgaaa aaaatcagta cagcatcctg tgggtggagc accaggacaa gggacggctt
     5881 gagctgaatt ttgtcattcc gaacatggag ctacagaccg gaaaacgcct ccagccgtac
     5941 tacgaccgcg cagacaggcc tagaattgat gcctggcaga cgctggtgaa tcaccattac
     6001 gggctgcatg acccgaacgc cccggagaac cgcaggacgc tgacactccc tgataacctg
     6061 cctgaaacga aacaggcgct tgctgagggc gtcacgcgag gtatagatgc actttaccat
     6121 gccggagaga taaaaggccg tcaggatgtg attcaggcgc tcactgaggc ggggctggaa
     6181 gtggtcaggg tgacgcgaag cagtatcagc attgcagatc cgaacggcgg gaagaatatc
     6241 aggctgaaag gagcatttta tgagcaatct tttgcagacg ggcgcggagt tcgagaaaaa
     6301 gctgaaagag agagccgaat ctacagagaa aatgctgaac aacgagttca ggaggctcgg
     6361 cgaatctgta agcgaggctg tgacatcaaa cgagacgaaa atcagagacg ctatagccct
     6421 gttcacagcc tcgacagagg aatcgctgga aaaacaccgg gaaggggtga aagaggcgat
     6481 gatgcagcac aggagggacg tgttaaagct ggcagggaat acgggcatga tgttactggg
     6541 gatagtcttt ctcctgttta ccgcgagtgg cgggacgctc tggtatcttg gagggaggat
     6601 acaggcgaac ctggaagaaa tcaggaagca ggaagagaca ttgcagaaac tgaacgcgaa
     6661 gacatgggg
//
