LOCUS       NZ_CP056971             7424 bp    DNA     circular CON 02-APR-2024
DEFINITION  Escherichia fergusonii strain RHB41-C15 plasmid pRHB41-C15_4,
            complete sequence.
ACCESSION   NZ_CP056971
VERSION     NZ_CP056971.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148427
            Assembly: GCF_013801845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T3-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.040080997967x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013801845.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 04:22:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,824
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,567
            CDSs (with protein)               :: 4,567
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 54 of 134
            Pseudo Genes (incomplete)         :: 73 of 134
            Pseudo Genes (internal stop)      :: 23 of 134
            Pseudo Genes (multiple problems)  :: 17 of 134
            Pseudo Genes (short protein)      :: 2 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056971.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB41-C15"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB41-C15_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            308..826
                     /locus_tag="HV010_RS23885"
                     /old_locus_tag="HV010_23880"
     CDS             308..826
                     /locus_tag="HV010_RS23885"
                     /old_locus_tag="HV010_23880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            833..1066
                     /locus_tag="HV010_RS23890"
                     /old_locus_tag="HV010_23885"
     CDS             833..1066
                     /locus_tag="HV010_RS23890"
                     /old_locus_tag="HV010_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1384..1803)
                     /locus_tag="HV010_RS23895"
                     /old_locus_tag="HV010_23890"
     CDS             complement(1384..1803)
                     /locus_tag="HV010_RS23895"
                     /old_locus_tag="HV010_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1884..2321)
                     /locus_tag="HV010_RS23900"
                     /old_locus_tag="HV010_23895"
     CDS             complement(1884..2321)
                     /locus_tag="HV010_RS23900"
                     /old_locus_tag="HV010_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2581..>3960
                     /gene="cea"
                     /locus_tag="HV010_RS23905"
                     /old_locus_tag="HV010_23900"
                     /pseudo=""
     CDS             2581..>3960
                     /gene="cea"
                     /locus_tag="HV010_RS23905"
                     /old_locus_tag="HV010_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /name="cea"
     gene            4015..4712
                     /locus_tag="HV010_RS23910"
                     /old_locus_tag="HV010_23905"
     CDS             join(4015..4264,4264..4712)
                     /locus_tag="HV010_RS23910"
                     /old_locus_tag="HV010_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            <4729..4926
                     /locus_tag="HV010_RS23915"
                     /old_locus_tag="HV010_23910"
                     /pseudo=""
     CDS             <4729..4926
                     /locus_tag="HV010_RS23915"
                     /old_locus_tag="HV010_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /name="colicin-like pore-forming protein"
     gene            complement(4923..5264)
                     /locus_tag="HV010_RS23920"
                     /old_locus_tag="HV010_23915"
     CDS             complement(4923..5264)
                     /locus_tag="HV010_RS23920"
                     /old_locus_tag="HV010_23915"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            5312..5449
                     /locus_tag="HV010_RS23925"
                     /old_locus_tag="HV010_23920"
     CDS             5312..5449
                     /locus_tag="HV010_RS23925"
                     /old_locus_tag="HV010_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(5648..5806)
                     /locus_tag="HV010_RS24365"
     CDS             complement(5648..5806)
                     /locus_tag="HV010_RS24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(6476..6667)
                     /locus_tag="HV010_RS23930"
                     /old_locus_tag="HV010_23925"
     CDS             complement(6476..6667)
                     /locus_tag="HV010_RS23930"
                     /old_locus_tag="HV010_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6731..7054
                     /locus_tag="HV010_RS23935"
                     /old_locus_tag="HV010_23930"
     CDS             6731..7054
                     /locus_tag="HV010_RS23935"
                     /old_locus_tag="HV010_23930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          6304..6391
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5735..6290)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..1170
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.237"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7045..7424
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.213"
                     /coverage="100.0"
                     /evalue="1.22e-73"
ORIGIN
        1 gacaggccta gaattgatgc ctggcagacg ctggtgaatc accattacgg gctgcatgac
       61 ccgaacgccc cggagaaccg caggacgttg acactccctg ataacctgcc tgaaacgaaa
      121 caggcgcttg ctgagggcgt cacgcgaggt atagatgcac tttaccatgc cggagagata
      181 aaaggccgtc aggatgtgat tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg
      241 acgcgaagca gtatcagcat tgcagatccg aacggcggga agaatatcag gctgaaagga
      301 gcattttatg agcaatcttt tgcagacggg cgcggagttc gagaaaaagc tgaaagagag
      361 agccgaatct acagagaaaa tgctgaacaa cgagttcagg aggctcggcg aatctgtaag
      421 cgaggctgtg acatcaaacg agacgaaaat cagagacgct atagccctgt tcacagcctc
      481 gacagaggaa tcgctggaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
      541 gagggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tagtctttct
      601 cctgtttacc gcgagtggcg ggacgctctg gtatcttgga gggaggatac aggcgaacct
      661 ggaagaaatc aggaagcagg aagagacatt gcagaaactg aacgcgaaga catggggcgt
      721 ggagtttgtg caggacggga acaggaaatt ccttgtcctt ccgtacggga aatcagcgga
      781 ggtgattccc tttcagggga aagagtgggt acatctgaag gagtgacaca gagtgacaga
      841 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
      901 gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg ttttcgctta cgcaacggga
      961 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgcagc acttgagcga
     1021 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
     1081 atacaggagc gcgaacatgt gttatccctg gaacgggagc gtcagccgga aatacaggaa
     1141 cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
     1201 attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa
     1261 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
     1321 tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc
     1381 cacttactgt ctgccctcgt agccatcgag ataaaccgca gaaatcgtgt cagccagcag
     1441 cctggatttt ttcggggtaa ggtctttgct gcggtaatca gtacccgcaa attgagttgt
     1501 gccccggagt gagtttaaaa attcctggct gtgaaactgc tgaatgcgtt gttgcacatc
     1561 actccgtgat tttcctgtcg ctttatcttt agctcccata tcccagaact gccgaaaaaa
     1621 aggcaccatc attcgtgttg tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc
     1681 atcagaagca tatacgtaat gccgggtatg ttgttccacg ctgccctttg gggtacaggc
     1741 tgtgagagct actgttgctg ttatcatggc gataagacaa agtttgtttc cggtacgtct
     1801 catgagtgtc tccctacctt aaagtattta ttctcggtga gttattatcc gaagactatt
     1861 ttatcatcag tctctgacag cgttcagcga ccgaacaacc tggaccagaa tccccgtttc
     1921 ttttccagtt ccagtgcctg cctgagctgg gctatctcat tttgtagctg ttcccgttct
     1981 gcttcctggc gtctgcgatc catatcctgt gcctgtttat cctcaagcat cagcgtcagg
     2041 cattgtttca gctcattcag ctcccggaga atgcgttctg tctgctgatc atgtggattt
     2101 tctgcatgtc cctcactgtg cctttctggt gtctcattct gctttaattc gccgtatgcc
     2161 cggatcagtt cactggtttc aaactcccgt cgtccgtccc tgccggtacg gtaactgaca
     2221 aggcctgcgc gcatatcacg gtaaaactgt gaacgcgatc tgcctgtcat ttttagtgcg
     2281 tcccggagtg tgtgccatgc cataaagtga cagtgtccca tagatgtctc atctcatagt
     2341 ttcagtaaaa cataatgagg tctgagaacg gtaatgtttg tgctggtttt tgtggcatcg
     2401 ggcgagaata gcgcgtggtg tgaaagactg tttttttgat cgttttcaca aaaatggaag
     2461 tccacagtct tgacagggaa aatgcagcgg cgtagctttt atgctgtata taaaaccagt
     2521 ggttatatgt acagcattta tttttaactt attgttttaa aagtcaaaga ggattttata
     2581 atggaaaccg cggtagcgta ctataaagat ggtgttcctt atgatgataa gggacaggta
     2641 attattactc ttttgaatgg tactcctgac gggagtggct ctggcggcgg aggtggaaaa
     2701 ggaggcagta aaagtgaaag ttctgcagct attcatgcaa ctgctaaatg gtctactgct
     2761 caattaaaga aaacacaggc agagcaggct gcccgggcaa aagctgcagc ggaagcacag
     2821 gcgaaagcaa aggcaaacag ggatgcgctg actcagcgcc tgaaggatat cgtgaatgag
     2881 gctcttcgtc acaatgcctc acgtacgcct tcagcaacag agcttgctca tgctaataat
     2941 gcagctatgc aggcggaagc agagcgtttg cgccttgcga aagcagaaga aaaagcccgt
     3001 aaagaagcgg aagcagcaga aaaggctttt caggaagcag aacaacgacg taaagagatt
     3061 gaacgggaga aggctgaaac agaacgccag ttgaaactgg ctgaagctga agagaaacga
     3121 ctggctgcat tgagtgaaga agctaaagct gttgagatcg cccaaaaaaa actttctgct
     3181 gcacaatctg aagtggtgaa aatggatgga gagattaaga ctctcaattc tcgtttaagc
     3241 tccagtatcc atgcccgtga tgcagaaatg aaaacgctcg ctggaaaacg aaatgaactg
     3301 gctcaggcat ccgctaaata taaagaactg gatgagctgg tcaaaaaact atcaccaaga
     3361 gccaatgatc cgcttcagaa ccgtcctttt tttgaagcaa ccagacgacg ggttggggcc
     3421 ggtaagatta gagaagaaaa acaaaaacag gtaacagcat cagaaacacg tattaaccgg
     3481 ataaatgctg atataactca gatccagaag gctatttctc aggtcagtaa taatcgtaat
     3541 gccggtatcg ctcgtgttca tgaagctgaa gaaaatttga aaaaagcaca gaataatctc
     3601 cttaattcac agattaagga tgctgttgat gcaacagtta gcttttatca aacgctgact
     3661 gaaaaatatg gtgaaaaata ttcgaaaatg gcacaggaac ttgctgataa gtctaaaggt
     3721 aagaaaatcg gcaatgtgaa tgaagctctc gctgcttttg aaaaatacaa ggatgtttta
     3781 aataagaaat tcagcaaagc cgatcgtgat gctattttta atgcgttggc atcggtgaag
     3841 tatgatgact gggctaaaca tttagatcag tttgccaagt acttgaagat tacggggcat
     3901 gtttcttttg gatatgatgt ggtatctgat atcctaaaaa ttaaggatac aggtgactgg
     3961 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     4021 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     4081 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
     4141 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
     4201 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttccgc
     4261 catttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca
     4321 tcgtctgcgc ggaaatggac gaacagtggg gatacgtcgg ggctaaatcg cgccagcgct
     4381 ggctgtttta cgcgtatgac aggctccgga agacggttgt tgcgcacgta ttcggtgaac
     4441 gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat
     4501 ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca
     4561 gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac
     4621 ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg
     4681 ggcattatct gaacataaaa cactatcaat aagtttgagt cattaccagg tgactggaag
     4741 ccactatttc ttacattaga gaagaaagct gcagatgcag gggtgagtta tgttgttgct
     4801 ttacttttta gcttgcttgc tggaactaca ttaggtattt ggggtattgc tattgttaca
     4861 ggaattctat gctcctatat tgataagaat aaacttaata ctataaatga ggtgttaggg
     4921 atttaattat tctttattga tataaaaagt cctagcaatc caaatgggat tgctaggacc
     4981 aaacaaagta gattatatag cataaatagg tttaattttg ctacgggggc gttatttagg
     5041 ttttttcttc tttcgaaaaa atctttcttt atgaagttaa aagctatgta ttcaatagca
     5101 tattttgaat atggacatag aatagtgctt atcactattg catatagcat cttatctgac
     5161 acaaggaaat aatacccttc gctgtttttt gttataaggt atatatatat aagtgtgcag
     5221 tacaggccaa ataaaatatt ttttatgtag tatcttaagc tcataaatta aacctcgcca
     5281 tatattcttt tcattttata aggatcgagt tatgaggaaa agattttttg tgggaatatt
     5341 cgcgataaac ctccttgttg gatgtcaggc taactatata cgtgatgttc agggagggac
     5401 catcgcacca tcctcctctt ctaaactgac ggggatcgcg gttcagtaga aaagatcaaa
     5461 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5521 ccgctaccaa cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5581 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagtcgggc
     5641 cactacttca agaactctgt agcaccgttt gtgccatcat cgctctgcta atccggttac
     5701 cagtggctgc tgccagtggc gttaaggcgt gccttaccgg gttggactca agacgatagt
     5761 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5821 agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc
     5881 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5941 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6001 acctctgact tgagcgtcta tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6061 acgcctgcta cgtggccttc ttcctgttcc tggtcttttg ctcacatgtt ctttccggcc
     6121 ttatcccctg attctgtgga taactgtgtt accgtttttg tgtgagtcag taccgctcgc
     6181 cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaaa gcgcctggac
     6241 gtgcattttc tccttacgca tctgtgcggc atttcacacc cggcatggcg tacttttcat
     6301 acaatccgca ctgatgccgc atggttaagc cagtatacac tccgctatcg ctacgtgact
     6361 gggtcagggc tgcgccccga cacccgctaa aacctgctga cgcgccctga cgggcttgtc
     6421 agctcccggc atccgctcac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6481 ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca tcggcgtggt
     6541 cgtgaagcga ttcacaaata tcggcctgtt catctgcgtc cagttcgttg agcttctcca
     6601 gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     6661 tagtcactga tgcctccgtg taagggggat ttctgttcat ggggtaatga taccgatgaa
     6721 acgcgagagg atgctcacaa tacgggttac tgatgatgaa catgcccggt tacttgaacg
     6781 ctgtgagggt aaacaactgg cggtatggat gcggcgggtc tgcctggggg agccggttgc
     6841 ccgttccgga aaactgccga cactggcacc gccgttactg cgtcagctgg ccgccatcgg
     6901 aaataacctg aatcagacag cccgtaaggt gaacagcggg cagtggtctt ccggtgaccg
     6961 ggttcaggtg gtggccgcac tgatggccat cggggatgag ctgcgccggc tgcgtctggc
     7021 tgtcagggaa cagggggcgc gggatgatag ttaaatttca tgccagggga aaaggtggtg
     7081 gcagtggtcc ggttgattac ctgctgggga gggagcgtaa ccgcgaaggc gcaacggtgc
     7141 ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc cacgccattt gcgaagaaat
     7201 acacgtccgg tgttctgtcg ttcgcggaga aggagctgcc gccgggagga cgtgaaaaag
     7261 tgatggcgag ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag tacagcatcc
     7321 tgtgggtgga gcaccaggac aagggacggc ttgagctgaa ttttgtcatt ccgaacatgg
     7381 agctacagac cggaaaacgc ctccagccgt actacgaccg cgca
//
