LOCUS       NZ_CP056964             7424 bp    DNA     circular CON 02-APR-2024
DEFINITION  Escherichia fergusonii strain RHB41-C20 plasmid pRHB41-C20_4,
            complete sequence.
ACCESSION   NZ_CP056964
VERSION     NZ_CP056964.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148429
            Assembly: GCF_013801425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T3-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132.253115999787x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013801425.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 04:40:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,826
            CDSs (total)                      :: 4,703
            Genes (coding)                    :: 4,568
            CDSs (with protein)               :: 4,568
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 54 of 135
            Pseudo Genes (incomplete)         :: 74 of 135
            Pseudo Genes (internal stop)      :: 23 of 135
            Pseudo Genes (multiple problems)  :: 17 of 135
            Pseudo Genes (short protein)      :: 2 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056964.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB41-C20"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB41-C20_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(7269..7424,1..36)
                     /locus_tag="HV012_RS23880"
                     /old_locus_tag="HV012_23885"
     CDS             join(7269..7424,1..36)
                     /locus_tag="HV012_RS23880"
                     /old_locus_tag="HV012_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            706..864
                     /locus_tag="HV012_RS24370"
     CDS             706..864
                     /locus_tag="HV012_RS24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            1362..1589
                     /locus_tag="HV012_RS23890"
                     /old_locus_tag="HV012_23895"
     CDS             1362..1589
                     /locus_tag="HV012_RS23890"
                     /old_locus_tag="HV012_23895"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1586..>1783)
                     /locus_tag="HV012_RS23895"
                     /old_locus_tag="HV012_23900"
                     /pseudo=""
     CDS             complement(1586..>1783)
                     /locus_tag="HV012_RS23895"
                     /old_locus_tag="HV012_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /name="colicin-like pore-forming protein"
     gene            complement(1800..2497)
                     /locus_tag="HV012_RS23900"
                     /old_locus_tag="HV012_23905"
     CDS             complement(join(1800..2248,2248..2497))
                     /locus_tag="HV012_RS23900"
                     /old_locus_tag="HV012_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<2552..3931)
                     /gene="cea"
                     /locus_tag="HV012_RS23905"
                     /old_locus_tag="HV012_23910"
                     /pseudo=""
     CDS             complement(<2552..3931)
                     /gene="cea"
                     /locus_tag="HV012_RS23905"
                     /old_locus_tag="HV012_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /name="cea"
     gene            4191..4628
                     /locus_tag="HV012_RS23910"
                     /old_locus_tag="HV012_23915"
     CDS             4191..4628
                     /locus_tag="HV012_RS23910"
                     /old_locus_tag="HV012_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4709..5128
                     /locus_tag="HV012_RS23915"
                     /old_locus_tag="HV012_23920"
     CDS             4709..5128
                     /locus_tag="HV012_RS23915"
                     /old_locus_tag="HV012_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5446..5679)
                     /locus_tag="HV012_RS23920"
                     /old_locus_tag="HV012_23925"
     CDS             complement(5446..5679)
                     /locus_tag="HV012_RS23920"
                     /old_locus_tag="HV012_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5686..6204)
                     /locus_tag="HV012_RS23925"
                     /old_locus_tag="HV012_23930"
     CDS             complement(5686..6204)
                     /locus_tag="HV012_RS23925"
                     /old_locus_tag="HV012_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6242..6940)
                     /locus_tag="HV012_RS24375"
                     /old_locus_tag="HV012_23935"
                     /pseudo=""
     CDS             complement(<6242..6940)
                     /locus_tag="HV012_RS24375"
                     /old_locus_tag="HV012_23935"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6882..7205)
                     /locus_tag="HV012_RS23935"
                     /old_locus_tag="HV012_23940"
     CDS             complement(6882..7205)
                     /locus_tag="HV012_RS23935"
                     /old_locus_tag="HV012_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(122..209)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          220..778
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5343..6892)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtgagcgga tgccgggagc tgacaagccc gtcagggcgc gtcagcaggt
      121 tttagcgggt gtcggggcgc agccctgacc cagtcacgta gcgatagcgg agtgtatact
      181 ggcttaacca tgcggcatca gtgcggattg tatgaaaagt acgccatgcc gggtgtgaaa
      241 tgccgcacag atgcgtaagg agaaaatgca cgtccaggcg cttttccgct tcctcgctca
      301 ctgactcgct acgctcggtc gttcgactgc ggcgagcggt actgactcac acaaaaacgg
      361 taacacagtt atccacagaa tcaggggata aggccggaaa gaacatgtga gcaaaagacc
      421 aggaacagga agaaggccac gtagcaggcg tttttccata ggctccgccc ccctgacgag
      481 catcacaaaa atagacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      661 tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaagg
      781 cacgccttaa cgccactggc agcagccact ggtaaccgga ttagcagagc gatgatggca
      841 caaacggtgc tacagagttc ttgaagtagt ggcccgacta cggctacact agaaggacag
      901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      961 gatccggcaa acaaaccacc gttggtagcg gtggtttttt tgtttgcaag cagcagatta
     1021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc
     1081 cgtcagttta gaagaggagg atggtgcgat ggtccctccc tgaacatcac gtatatagtt
     1141 agcctgacat ccaacaagga ggtttatcgc gaatattccc acaaaaaatc ttttcctcat
     1201 aactcgatcc ttataaaatg aaaagaatat atggcgaggt ttaatttatg agcttaagat
     1261 actacataaa aaatatttta tttggcctgt actgcacact tatatatata taccttataa
     1321 caaaaaacag cgaagggtat tatttccttg tgtcagataa gatgctatat gcaatagtga
     1381 taagcactat tctatgtcca tattcaaaat atgctattga atacatagct tttaacttca
     1441 taaagaaaga ttttttcgaa agaagaaaaa acctaaataa cgcccccgta gcaaaattaa
     1501 acctatttat gctatataat ctactttgtt tggtcctagc aatcccattt ggattgctag
     1561 gactttttat atcaataaag aataattaaa tccctaacac ctcatttata gtattaagtt
     1621 tattcttatc aatataggag catagaattc ctgtaacaat agcaataccc caaataccta
     1681 atgtagttcc agcaagcaag ctaaaaagta aagcaacaac ataactcacc cctgcatctg
     1741 cagctttctt ctctaatgta agaaatagtg gcttccagtc acctggtaat gactcaaact
     1801 tattgatagt gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat
     1861 tttgagaacg acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta
     1921 tgccgctcaa ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg
     1981 gattcataca gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg
     2041 ctcataagac gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc
     2101 ttccggagcc tgtcatacgc gtaaaacagc cagcgctggc gcgatttagc cccgacgtat
     2161 ccccactgtt cgtccatttc cgcgcagacg atgacgtcac tgcccggctg tatgcgcgag
     2221 gttaccgact gcggcctgag ttttttaaat ggcggaaaat cgtgttgagg ccaacgccca
     2281 taatgcgggc tgttgcccgg catccaacgc cattcatggc catatcaatg attttctggt
     2341 gcgtaccggg ttgagaagcg gtgtaagtga actgcagttg ccatgtttta cggcagtgag
     2401 agcagagata gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag
     2461 ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc
     2521 atacactaaa tcagtaagtt ggcagcatca cccagtcacc tgtatcctta atttttagga
     2581 tatcagatac cacatcatat ccaaaagaaa catgccccgt aatcttcaag tacttggcaa
     2641 actgatctaa atgtttagcc cagtcatcat acttcaccga tgccaacgca ttaaaaatag
     2701 catcacgatc ggctttgctg aatttcttat ttaaaacatc cttgtatttt tcaaaagcag
     2761 cgagagcttc attcacattg ccgattttct tacctttaga cttatcagca agttcctgtg
     2821 ccattttcga atatttttca ccatattttt cagtcagcgt ttgataaaag ctaactgttg
     2881 catcaacagc atccttaatc tgtgaattaa ggagattatt ctgtgctttt ttcaaatttt
     2941 cttcagcttc atgaacacga gcgataccgg cattacgatt attactgacc tgagaaatag
     3001 ccttctggat ctgagttata tcagcattta tccggttaat acgtgtttct gatgctgtta
     3061 cctgtttttg tttttcttct ctaatcttac cggccccaac ccgtcgtctg gttgcttcaa
     3121 aaaaaggacg gttctgaagc ggatcattgg ctcttggtga tagttttttg accagctcat
     3181 ccagttcttt atatttagcg gatgcctgag ccagttcatt tcgttttcca gcgagcgttt
     3241 tcatttctgc atcacgggca tggatactgg agcttaaacg agaattgaga gtcttaatct
     3301 ctccatccat tttcaccact tcagattgtg cagcagaaag ttttttttgg gcgatctcaa
     3361 cagctttagc ttcttcactc aatgcagcca gtcgtttctc ttcagcttca gccagtttca
     3421 actggcgttc tgtttcagcc ttctcccgtt caatctcttt acgtcgttgt tctgcttcct
     3481 gaaaagcctt ttctgctgct tccgcttctt tacgggcttt ttcttctgct ttcgcaaggc
     3541 gcaaacgctc tgcttccgcc tgcatagctg cattattagc atgagcaagc tctgttgctg
     3601 aaggcgtacg tgaggcattg tgacgaagag cctcattcac gatatccttc aggcgctgag
     3661 tcagcgcatc cctgtttgcc tttgctttcg cctgtgcttc cgctgcagct tttgcccggg
     3721 cagcctgctc tgcctgtgtt ttctttaatt gagcagtaga ccatttagca gttgcatgaa
     3781 tagctgcaga actttcactt ttactgcctc cttttccacc tccgccgcca gagccactcc
     3841 cgtcaggagt accattcaaa agagtaataa ttacctgtcc cttatcatca taaggaacac
     3901 catctttata gtacgctacc gcggtttcca ttataaaatc ctctttgact tttaaaacaa
     3961 taagttaaaa ataaatgctg tacatataac cactggtttt atatacagca taaaagctac
     4021 gccgctgcat tttccctgtc aagactgtgg acttccattt ttgtgaaaac gatcaaaaaa
     4081 acagtctttc acaccacgcg ctattctcgc ccgatgccac aaaaaccagc acaaacatta
     4141 ccgttctcag acctcattat gttttactga aactatgaga tgagacatct atgggacact
     4201 gtcactttat ggcatggcac acactccggg acgcactaaa aatgacaggc agatcgcgtt
     4261 cacagtttta ccgtgatatg cgcgcaggcc ttgtcagtta ccgtaccggc agggacggac
     4321 gacgggagtt tgaaaccagt gaactgatcc gggcatacgg cgaattaaag cagaatgaga
     4381 caccagaaag gcacagtgag ggacatgcag aaaatccaca tgatcagcag acagaacgca
     4441 ttctccggga gctgaatgag ctgaaacaat gcctgacgct gatgcttgag gataaacagg
     4501 cacaggatat ggatcgcaga cgccaggaag cagaacggga acagctacaa aatgagatag
     4561 cccagctcag gcaggcactg gaactggaaa agaaacgggg attctggtcc aggttgttcg
     4621 gtcgctgaac gctgtcagag actgatgata aaatagtctt cggataataa ctcaccgaga
     4681 ataaatactt taaggtaggg agacactcat gagacgtacc ggaaacaaac tttgtcttat
     4741 cgccatgata acagcaacag tagctctcac agcctgtacc ccaaagggca gcgtggaaca
     4801 acatacccgg cattacgtat atgcttctga tgacggtttt gatcccaact tttccaccca
     4861 aaaagccgac acaacacgaa tgatggtgcc tttttttcgg cagttctggg atatgggagc
     4921 taaagataaa gcgacaggaa aatcacggag tgatgtgcaa caacgcattc agcagtttca
     4981 cagccaggaa tttttaaact cactccgggg cacaactcaa tttgcgggta ctgattaccg
     5041 cagcaaagac cttaccccga aaaaatccag gctgctggct gacacgattt ctgcggttta
     5101 tctcgatggc tacgagggca gacagtaagt ggatttacca taatccctta attgtacgca
     5161 ccgctaaaac gcgttcagcg cgatcacggc agcagacagg taaaaatggc aacaaaccac
     5221 ccgaaaaact gccgcgatcg cgcctgataa attttaaccg tatgaatacc tatgcaacca
     5281 gagggtacag gccacattac ccccacttaa tccactgaag ctgccatttt tcatggtttc
     5341 accatcccag cgaagggcca tccagcgtgc gttcctgtat ttccggctga cgctcccgtt
     5401 ccagggataa cacatgttcg cgctcctgta tcagccgttc ctctcttatc tccagttctc
     5461 gctgtataac tggctcaagc gttctgtctg ctcgctcaag tgctgcacct gctgactcaa
     5521 ctgcatgacc cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa acatcttctg
     5581 caattccacg aaggcgctct cccattcgct cagccgctgc atatagtcct gttgcagctg
     5641 ctctaaggcg ttcagcaaat gtgtttccag ctctgtcact ctgtgtcact ccttcagatg
     5701 tacccactct ttcccctgaa agggaatcac ctccgctgat ttcccgtacg gaaggacaag
     5761 gaatttcctg ttcccgtcct gcacaaactc cacgccccat gtcttcgcgt tcagtttctg
     5821 caatgtctct tcctgcttcc tgatttcttc caggttcgcc tgtatcctcc ctccaagata
     5881 ccagagcgtc ccgccactcg cggtaaacag gagaaagact atccccagta acatcatgcc
     5941 cgtattccct gccagcttta acacgtccct cctgtgctgc atcatcgcct ctttcacccc
     6001 ttcccggtgt ttttccagcg attcctctgt cgaggctgtg aacagggcta tagcgtctct
     6061 gattttcgtc tcgtttgatg tcacagcctc gcttacagat tcgccgagcc tcctgaactc
     6121 gttgttcagc attttctctg tagattcggc tctctctttc agctttttct cgaactccgc
     6181 gcccgtctgc aaaagattgc tcataaaatg ctcctttcag cctgatattc ttcccgccgt
     6241 tcggatctgc aatgctgata ctgcttcgcg tcaccctgac cacttccagc cccgcctcag
     6301 tgagcgcctg aatcacatcc tgacggcctt ttatctctcc ggcatggtaa agtgcatcta
     6361 tacctcgcgt gacgccctca gcaagcgcct gtttcgtttc aggcaggtta tcagggagtg
     6421 tcaacgtcct gcggttctcc ggggcgttcg ggtcatgcag cccgtaatgg tgattcacca
     6481 gcgtctgcca ggcatcaatt ctaggcctgt ctgcgcggtc gtagtacggc tggaggcgtt
     6541 ttccggtctg tagctccatg ttcggaatga caaaattcag ctcaagccgt cccttgtcct
     6601 ggtgctccac ccacaggatg ctgtactgat ttttttcgag accgggcatc agtacacgct
     6661 caaagctcgc catcactttt tcacgtcctc ccggcggcag ctccttctcc gcgaacgaca
     6721 gaacaccgga cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt
     6781 ccggattacc ctgaagcacc gttgcgcctt cgcggttacg ctccctcccc agcaggtaat
     6841 caaccggacc actgccacca ccttttcccc tggcatgaaa tttaactatc atcccgcgcc
     6901 ccctgttccc tgacagccag acgcagccgg cgcagctcat ccccgatggc catcagtgcg
     6961 gccaccacct gaacccggtc accggaagac cactgcccgc tgttcacctt acgggctgtc
     7021 tgattcaggt tatttccgat ggcggccagc tgacgcagta acggcggtgc cagtgtcggc
     7081 agttttccgg aacgggcaac cggctccccc aggcagaccc gccgcatcca taccgccagt
     7141 tgtttaccct cacagcgttc aagtaaccgg gcatgttcat catcagtaac ccgtattgtg
     7201 agcatcctct cgcgtttcat cggtatcatt accccatgaa cagaaatccc ccttacacgg
     7261 aggcatcagt gactaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
     7321 agacattaac gctgctggag aagctcaacg aactggacgc agatgaacag gccgatattt
     7381 gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ctgc
//
