LOCUS       NZ_CP056901             2746 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Citrobacter sp. RHBSTW-00017 plasmid pRHBSTW-00017_3, complete
            sequence.
ACCESSION   NZ_CP056901
VERSION     NZ_CP056901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148454
            Assembly: GCF_013797615.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00017
  ORGANISM  Citrobacter sp. RHBSTW-00017
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.4630527150885x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013797615.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:24:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,916
            CDSs (total)                      :: 4,798
            Genes (coding)                    :: 4,637
            CDSs (with protein)               :: 4,637
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 41 of 161
            Pseudo Genes (incomplete)         :: 121 of 161
            Pseudo Genes (internal stop)      :: 31 of 161
            Pseudo Genes (multiple problems)  :: 27 of 161
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Citrobacter sp. RHBSTW-00017"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00017"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742629"
                     /plasmid="pRHBSTW-00017_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2671..2746,1..56))
                     /locus_tag="HV037_RS24370"
                     /old_locus_tag="HV037_24370"
     CDS             complement(join(2671..2746,1..56))
                     /locus_tag="HV037_RS24370"
                     /old_locus_tag="HV037_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052684121.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDLTPLAAAIGKRIHGAG"
                     /name="hypothetical protein"
     gene            910..1137
                     /locus_tag="HV037_RS24375"
                     /old_locus_tag="HV037_24375"
     CDS             910..1137
                     /locus_tag="HV037_RS24375"
                     /old_locus_tag="HV037_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223229632.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHDGYT"
                     /name="hypothetical protein"
     gene            complement(1185..1373)
                     /locus_tag="HV037_RS24380"
                     /old_locus_tag="HV037_24380"
     CDS             complement(1185..1373)
                     /locus_tag="HV037_RS24380"
                     /old_locus_tag="HV037_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1702..2076
                     /locus_tag="HV037_RS24385"
                     /old_locus_tag="HV037_24385"
     CDS             1702..2076
                     /locus_tag="HV037_RS24385"
                     /old_locus_tag="HV037_24385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            complement(2265..>2366)
                     /locus_tag="HV037_RS24390"
                     /old_locus_tag="HV037_24390"
                     /pseudo=""
     CDS             complement(2265..>2366)
                     /locus_tag="HV037_RS24390"
                     /old_locus_tag="HV037_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     Typing          complement(2..441)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.57"
                     /coverage="84.0"
                     /evalue="0.0"
ORIGIN
        1 agacgatagt taccggacaa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag
       61 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagataaacg
      121 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
      181 ggagccacca gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccaccactga
      241 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttc
      301 gccgtggcct tcttgcttcc ctgttaagct tcttcctgga agatgccagg aaatttccgc
      361 cccgccagta agccggtacc gctcgccgca gccgaacgac cgagcggagc gagtcagtga
      421 gcgaggaagc ggaatatctc cgggcttatc tttggcaccg tacgcgccat agctgcattt
      481 tttaatacga tgcaggatag ggcaaacgcc gcaaaatgac gtcatccgac atcattccgc
      541 cgcgttcgtc cagtgaccta cagtcggctc tggggtgtcg gggctgtgcc ctgaccaggc
      601 gttttggagt atcgccaccg tggtggcggt gctacaaaac atatcctgtc ccttccttgg
      661 catctctgaa gtttagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
      721 gccccgacac ccgctaaaag cactgacgcg cctgcggctt gcccaacccc gcgccgtccg
      781 ggaaagaatt tcccgatcga cccgacgttt ctgtttacct gtgatgttaa cgtttggatc
      841 tttttcccgg gtttacctat tttcttctaa tgaagcgttc acagctgacg ttaatagatg
      901 ggtgctttca tgtttggttt ttcaccagga gaacttatga acctttgccc cgatgaacgt
      961 ctgctctttg tgcggatgat ttccgctatg cttcgccgtt cgggcggtga tgcgggtgct
     1021 gttatgtttg aggcgtatcg ccatattgtg tccgatacca atcaagctcg tcgttcctac
     1081 atgctcgacc tgctcgaaag cgtcaggcat gactatgtac acgatggata tacatagttg
     1141 ccctggatac gcttttcgtc taagtgagcg ttcttttcgt ctctttattc gccgtcctgt
     1201 ataccgaagc gtgcgctcag cttctgataa agcgcttctg cctgttcgtg cattcgctca
     1261 cagtcggctg cctcgtcatc aagatcgagc acgtcgagtt tttcgagcag cagcagcgac
     1321 tgcgcccgga taaattttgc catgttcagt gcggctgatt cctgttttgt catacggctt
     1381 tttcccggtt ttatggtgat cgcagactga aatcttactt cattcacttg agtgcatagc
     1441 catgcaggta cgcagaaatc atcagcgcgt ttctgagcgg ttttcagggg gtaagatgtg
     1501 ttcttttgca ggtgaggtcg tagaaacgcc ctgagagcct ctcaggcggt gcacgcaacg
     1561 gatgttatgt tcaatctggc ggtgcagcat agttatcatc gcacctacag accgcttgtt
     1621 gtattcagaa caatatttat ttgtataatt tgtattttgt tgtattattt cttcaaatta
     1681 gagcggtggg gtaataataa tatgaatggt atgtctgacg taagactaaa gaaatcaact
     1741 attgagagcc taaaagagta cgtgaatgac tttggcatga cggttgatga tgttgtcctg
     1801 aacttaattg aagagaataa ttcattaaaa tcgttattag gtcagggctt ttctttgcct
     1861 ggtggagata aggacacaca ttccttcaag gattacgtga ttcataagct tccaaaagga
     1921 aagatcttgg ttaccaaaga cgggcttaaa gttagcaaca tgaagaaggt attgatcgag
     1981 gcagggagcg ctgtagggat ggatgataaa gagctaaacc ctgactttac gacctatgaa
     2041 ataggccgtt ttctcttcaa acatcttcag ggataaaggt gtctaccagt tcgtggccgg
     2101 gattttaggt tcaattcagc tagttcccgg acagaaaaaa agccaccgtg taggtggctt
     2161 ttttattcct gctttaggca aagcattatt gatagcattt gcaagcaaac cgttagcgac
     2221 ttctgctgaa tgcttctggg cggattcaag ttcgaagtgc aacgctaagc cgcctgtctt
     2281 agctcagcaa agaccactga aggttgccgg aatccaagac atttccgtgg cctgctattc
     2341 agtgctctct ggtacttgcg gagctcccct gtgtgataag tcaggtgtgg aatttttacg
     2401 tataaggccc cgctaggcgg aaaccaatac tgaagccaaa tcactggtaa acagccggat
     2461 cttcttgtga tccttttact ctcgtcgtaa tctcatgtcc tgtaaacaaa aaaaccaccg
     2521 ctaccaacgg tggttcgttt gccggaatca agagctacca actctttctt ccgaaggtaa
     2581 ctggctttca cggagcgcag ataccaaaac tgtccttcca gtgtagccgc agtcaggccg
     2641 ccacttcaag aactctgtag aagcactcta ctaccctgct ccgtgaatcc tcttaccaat
     2701 ggctgctgcc aatggcgtta agtcgtgtct ttccgggttg gactca
//
