LOCUS       NZ_CP056897             8580 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Citrobacter sp. RHBSTW-00021 plasmid pRHBSTW-00021_2, complete
            sequence.
ACCESSION   NZ_CP056897
VERSION     NZ_CP056897.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148456
            Assembly: GCF_013797345.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00021
  ORGANISM  Citrobacter sp. RHBSTW-00021
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.6741630612516x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013797345.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:05:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,974
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 43 of 168
            Pseudo Genes (incomplete)         :: 128 of 168
            Pseudo Genes (internal stop)      :: 34 of 168
            Pseudo Genes (multiple problems)  :: 30 of 168
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056897.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Citrobacter sp. RHBSTW-00021"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00021"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742630"
                     /plasmid="pRHBSTW-00021_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(8180..8580,1..268)
                     /locus_tag="HV039_RS24590"
                     /old_locus_tag="HV039_24595"
     CDS             join(8180..8580,1..268)
                     /locus_tag="HV039_RS24590"
                     /old_locus_tag="HV039_24595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181631225.1"
                     /translation="MLKLRITSDDFQSLDKALQPFYVEQSDGYALNVEQDDTAAAGLKA
                     KNAELLTKLAGEKERRITAEAQLKVVNETVAGHDQKINQIAGERDSYRAGLEKSLIDEP
                     LTAFMRSNFIDGSDDFVVPVLKKFFQLHRNDDGTYESAIFINSDDGNGKKEVRADVEQL
                     RKHLHADGKLDWVLKASGTSGGGSTSSVSINSSTTHRYEPGPGGQQDLTSQAREIIGQK
                     "
                     /name="hypothetical protein"
     gene            298..1275
                     /locus_tag="HV039_RS24595"
                     /old_locus_tag="HV039_24600"
     CDS             298..1275
                     /locus_tag="HV039_RS24595"
                     /old_locus_tag="HV039_24600"
                     /inference="COORDINATES: protein motif:HMM:NF039452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="WP_181631226.1"
                     /translation="MSLEMFQNQVRSTITELVLKQSALFGAATGGAMMLGSEKTIGDYA
                     EESSWKLIAGLVTARDAYSTSAVTAKEIEQILKRAPRFDWRMGPVKVNDGLLARINSSP
                     EDAAAAVSAQAAQGIIEQQITQGLAALDACLSTNAKFSLAIAADPTAATGQITPKLSSF
                     VKGRRAFGDAGQNIICWAINSDVYYSLVENDLFKNAEQLYKLGDIAVFTDGLNGRFLVT
                     DYVPSNTAYGMVNGAVIIDNGYQSKFVAQPQLGGESLVTMMQSEGEFKIAVKSYRIKSS
                     LADTLANKVSITAAQVKDKANWEYFASDDSRAQPGVKLTFTPKA"
                     /name="major capsid protein"
     gene            1345..1791
                     /locus_tag="HV039_RS24600"
                     /old_locus_tag="HV039_24605"
     CDS             1345..1791
                     /locus_tag="HV039_RS24600"
                     /old_locus_tag="HV039_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017529439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626318.1"
                     /translation="MPENRDSEAGRDGKGRFQKGNSGNPGGVPAKTVALRKKLESGASK
                     AVAAVVAAAAEGDMSACRLILERVVPQTKPTYPPLSFELDDSDLPATARSILRAVADGL
                     LPTDQGKLLLDGLASMAKVIEVSELREMVERLEDEINSAKRDGY"
                     /name="hypothetical protein"
     gene            1963..2130
                     /locus_tag="HV039_RS24605"
                     /old_locus_tag="HV039_24610"
     CDS             1963..2130
                     /locus_tag="HV039_RS24605"
                     /old_locus_tag="HV039_24610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157845149.1"
                     /translation="MSLKARVEKLREQVQASATPDAVPVVFPGDPVPESGPYIAIQVVD
                     SRVRDDDESE"
                     /name="hypothetical protein"
     gene            2117..2299
                     /locus_tag="HV039_RS24610"
                     /old_locus_tag="HV039_24615"
     CDS             2117..2299
                     /locus_tag="HV039_RS24610"
                     /old_locus_tag="HV039_24615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181631227.1"
                     /translation="MSLSNRVRRLQQQVKQPEGAAVVMIISDVMNELGGDATPRTYTPA
                     QCREFDNWMRQFTND"
                     /name="hypothetical protein"
     gene            2292..2468
                     /locus_tag="HV039_RS24615"
                     /old_locus_tag="HV039_24620"
     CDS             2292..2468
                     /locus_tag="HV039_RS24615"
                     /old_locus_tag="HV039_24620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181631228.1"
                     /translation="MTKVMAAMMEELERLCIQYPDTFTAIEINVKGDDGEILHGFNIPS
                     EMLTEKDYSHATH"
                     /name="hypothetical protein"
     gene            2534..3127
                     /locus_tag="HV039_RS24620"
                     /old_locus_tag="HV039_24625"
     CDS             2534..3127
                     /locus_tag="HV039_RS24620"
                     /old_locus_tag="HV039_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="WP_181631229.1"
                     /translation="MARLTTIRLYGVLGAKFGRVHKLAVQTSAEAVKALCINFDGLEQY
                     LYDAKKNGMTFAVFRGKRNIGEQDFKELGGDSDIRIAPVMEGAKKAGLFQTILGAVMVV
                     AGIVVTGLSYGWATPVGGAMISAGIGMMAGGIYQMLSPQPKGLQGRDDPDNKPSYAFGG
                     SVNSLAMGNPVPVLYGEREIGGAIISAGIVAEDI"
                     /name="tail assembly protein"
     gene            3530..3694
                     /locus_tag="HV039_RS24625"
                     /old_locus_tag="HV039_24630"
     CDS             3530..3694
                     /locus_tag="HV039_RS24625"
                     /old_locus_tag="HV039_24630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157845150.1"
                     /translation="MATKTVQTTFRLTEDLMARLEQEAKRVRMETGENVTVPELLRRYA
                     ASLPEVKKP"
                     /name="hypothetical protein"
     gene            3895..4767
                     /locus_tag="HV039_RS24630"
                     /old_locus_tag="HV039_24635"
     CDS             3895..4767
                     /locus_tag="HV039_RS24630"
                     /old_locus_tag="HV039_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685904.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_181631230.1"
                     /translation="MNLQALTAKARAVRGNIVAAVSTKGSRTKSPVYERDEQIKLRERI
                     QQTQPEWVLLWWDISVVTGWRTSDVCNLRYSCIDWTTGKATITVAKQTKAAEARATRKG
                     VELVRKARKDACRLSGDHVGYMQWDSATADEIAAHMTAEEQEACFELVSRADVKRDTKQ
                     LPPGILKRLRERQERNLIDDDLVFSRSQIESNRCASLDGPITRQSVWRRLHTVCTWFMR
                     HVNAKLRLSAYSTRKIAAFNMMRRGGEQGLLIASEMLGHSNPAVTRTYLQLGSQAGELQ
                     AAMAMEVLA"
                     /name="tyrosine-type recombinase/integrase"
     gene            4764..5060
                     /locus_tag="HV039_RS24635"
                     /old_locus_tag="HV039_24640"
     CDS             4764..5060
                     /locus_tag="HV039_RS24635"
                     /old_locus_tag="HV039_24640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181631223.1"
                     /translation="MSIYNDFVRVNFPDVQGDDLLSLSGRAECAADSILNGVAAVGKMM
                     FYAGDIDNSVYEPSTADFSDIGGMLMELMPLVRVLTDTAANAECQHRQMAKRK"
                     /name="hypothetical protein"
     gene            5231..6196
                     /locus_tag="HV039_RS24640"
                     /old_locus_tag="HV039_24645"
     CDS             5231..6196
                     /locus_tag="HV039_RS24640"
                     /old_locus_tag="HV039_24645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181631224.1"
                     /translation="MAREFFIVDRDVFLRACERGMNAAVSYLVMARGTQGNNRTTKWSA
                     NAIAERAGMTRPRATAAIGELIDSGVVELVSGGARPHYRLHLPDSDNGYLYLPNSIIDA
                     AADETPPIERLRQTRNITNLIVFVLLYGIQDLDNSGGLPADMFDDTPSEAEVLYENKTM
                     RLEVVTKHTYKLSWAWYSRSSVFSLIEMDFNDFNSAFAELRDMGLVSVNAAVFDHHEYD
                     GEIIGSTHGYDYVEELDVAIRRKRKTMAKWPDGYYMIIPAHIKTACIKCVGVMRYRAQT
                     GKTARWVALSTKNGARELKLYNDALRLELALAIEREVRTA"
                     /name="hypothetical protein"
     gene            complement(7378..7905)
                     /locus_tag="HV039_RS24645"
                     /old_locus_tag="HV039_24650"
     CDS             complement(7378..7905)
                     /locus_tag="HV039_RS24645"
                     /old_locus_tag="HV039_24650"
                     /inference="COORDINATES: protein motif:HMM:NF041551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="WP_181631231.1"
                     /translation="MASKNVNGQSAVKNIRFPHQLLEDIAEAQGDLSFAAWVIDACARK
                     LAGGATPEISLNAAVKRLESIAQQFETIQERALPAIEVKPVKSISSKPVKKPAEPKTHT
                     VHTPGDNGADPRIAPGTFNAAVLELARTITSNHEIADRLNADGVSPGRGGELTHRVVMN
                     TKTRLKKWGYLD"
                     /name="YlcI/YnfO family protein"
ORIGIN
        1 tgacgacgga acctatgaga gtgccatttt catcaacagc gatgatggca acggcaagaa
       61 ggaagtaagg gcagacgtag aacagctgcg caagcactta cacgctgacg gcaaactcga
      121 ctgggttctg aaagcatcgg gaacaagcgg cggtggtagt acgtcctctg tcagtatcaa
      181 ttcatcaacc actcaccgct acgagccagg cccgggcgga cagcaagatc tcaccagtca
      241 ggctcgtgaa atcatcggac agaaataaca gtcaattaaa acgatgatag gtgaattatg
      301 tctttagaaa tgttccagaa tcaggttcgc tcaaccatta ccgagcttgt cctgaaacaa
      361 tccgcactgt tcggcgctgc cactggcggc gcaatgatgc tgggcagtga gaaaaccatt
      421 ggtgattatg ccgaggaatc cagctggaag ctgatcgccg ggctggttac tgcccgtgac
      481 gcttattcca cttctgctgt aacggcgaaa gaaatcgagc agatcctgaa acgtgcgccg
      541 cgctttgact ggcgcatggg gccggttaag gtgaatgacg ggttgctggc gcgtattaac
      601 tccagccctg aggacgcagc cgccgcagta tcggcacagg ccgcgcaggg catcattgag
      661 cagcagatca cgcaaggtct ggccgcgctg gatgcctgcc tttccacgaa cgcgaagttt
      721 tcactggcta ttgccgccga tcccacagcc gcaaccgggc aaatcactcc gaaactcagc
      781 agctttgtaa aagggcgccg ggcattcggt gacgctggcc agaatatcat ttgctgggcg
      841 attaactccg acgtgtatta cagcctggta gagaacgacc tgttcaagaa cgccgagcag
      901 ctctacaagc tgggtgacat tgccgtgttc actgatggcc tgaacgggcg tttccttgtc
      961 actgactacg ttccgtcaaa tacagcttat ggcatggtga acggcgctgt aatcattgat
     1021 aacggctacc agtccaagtt tgttgcacag ccgcagctgg gtggggaatc cctcgtcacg
     1081 atgatgcaga gcgaaggtga gttcaaaatc gctgttaaga gctaccgcat caaatcgtca
     1141 ctggccgaca cgctcgccaa taaggtatcg atcaccgccg cgcaggttaa ggacaaagcg
     1201 aactgggaat atttcgcctc tgacgacagc cgcgcacagc caggcgtcaa attgaccttt
     1261 acgcccaaag cataacccca taaccgttta gcatcacttg cccccgcatc cggtagtaca
     1321 gggggctttt ttataggtgg tcatatgcct gagaacaggg attctgaggc ggggcgtgac
     1381 ggcaaaggaa gattccagaa ggggaacagc ggcaaccccg gaggagtgcc agccaagacc
     1441 gtagccctgc gtaaaaagtt agagagtggc gcgagtaaag ccgtggccgc agtggtggcc
     1501 gcagccgcag aaggtgatat gtctgcatgt cgcctgattc tggaacgagt cgttccacaa
     1561 accaaaccaa cctacccgcc gttgtcgttc gaactggatg acagcgatct gccagccact
     1621 gcccggtcaa tcctccgcgc ggtcgctgat ggcctgttac caaccgacca ggggaaatta
     1681 cttctcgatg gcctggcatc aatggcaaaa gtcattgagg tgtcagagct gcgcgagatg
     1741 gtagaacgcc tggaagatga gattaacagc gccaagcgcg acgggtatta actgaggacg
     1801 gattatgtca attagagcca gagttaccag gctgaaagag tcagtaaaac gcgcaaatcc
     1861 cccggttcgc ttgattatgg agccgatccc cgcgctgacg gtggatgagt gggaagcatt
     1921 atcccagaag catcacgaaa agatgttgca gggaggtggt gcatgtcatt aaaagcgcgt
     1981 gtcgagaagc tgcgcgaaca ggtgcaggcg tccgcaacac ctgacgccgt tccggtagtg
     2041 ttccccggcg atccggttcc tgaatcaggc ccgtacatcg ccattcaggt ggttgatagc
     2101 cgtgtgaggg atgacgatga gtctgagtaa tcgagttcga cgcctgcaac agcaggtcaa
     2161 gcagccggaa ggggccgcag tggtgatgat tatcagcgac gtgatgaacg agctgggcgg
     2221 cgatgccacg ccccgcacct ataccccggc gcagtgccgg gagttcgata actggatgag
     2281 gcaattcacc aatgactaaa gttatggcgg ccatgatgga ggagctggag cgcctttgta
     2341 tccagtaccc ggacacattc accgcgattg aaattaacgt aaagggcgat gatggcgaga
     2401 tcctccacgg tttcaatatt ccatctgaaa tgctcacaga gaaggactac agccatgcta
     2461 cacactaact gttggtgcgt gatgtgcaga acgaaacagt ccaaaataac cgatccacag
     2521 gagggctaat tagatggcaa gattaaccac aattaggctg tatggcgttc ttggggcgaa
     2581 gttcgggcgc gtgcataaac tggcggtgca aacgtcagcg gaagcggtga aagcgttgtg
     2641 catcaatttc gacggtctgg agcaatacct ctatgacgca aagaaaaatg gcatgacctt
     2701 tgccgtgttt cgcggtaaac gcaacatcgg agaacaggat ttcaaggagc tgggtggtga
     2761 cagcgatatt cgtattgctc ctgtaatgga gggggcaaaa aaagcaggat tattccagac
     2821 gatccttggc gctgtaatgg tcgtcgcggg tatcgtggtg acaggccttt cgtacggctg
     2881 ggctacacca gtaggcggag ccatgatttc tgctggcatc ggtatgatgg ccggcggcat
     2941 ctaccagatg ctttctcccc agcccaaagg gctgcaaggc cgtgatgacc ccgacaacaa
     3001 gccaagctat gcgtttgggg gatctgtcaa ttcgctggcg atgggtaatc ctgttcccgt
     3061 tctctatggt gagcgtgaga tcgggggagc cattatcagt gctggtattg tggctgagga
     3121 tatttaaaca cgctcaatca tggcccgctc tcgggccatg acttcgcatt cgttaaggaa
     3181 tgcgaaatca tgcagatgta catacagtat gtacacaccc ccggcgcaaa attgcgctct
     3241 gctataaaaa caatacctgc atctgattcc ctgggatcaa cacaccttca agtctgtctc
     3301 ttagccgtgg gatttactca taattaaatc ccgtcagctt acgcgcgcct ctcgatatga
     3361 cgtccggtca gcacaaataa caggcaatta gccagcattc gcatgacgat ttctaatacg
     3421 tgatcacata tccagtattg ccccatgatt attttctatt agggtattgc atgtcacgga
     3481 gttaatggct atcttgaaca tcaagacaaa caaaggagaa taacaaacaa tggctaccaa
     3541 gaccgtgcag accactttcc gattgactga agatttgatg gctcgccttg agcaagaggc
     3601 aaagcgcgtt cggatggaaa ccggggagaa tgtgactgtg ccggagctat tgcgtcgtta
     3661 tgctgccagc ttgcctgaag tgaagaaacc ctaaaaagtc gaaaccccca gaccgcgcca
     3721 acggaactga gggttctaac ccaaaacgga gtacgccgtg atgagtatga tttcaatcat
     3781 aacaatcgcc ttgcgcattt gccagggcag aaccgtgaca atccccgcaa tgagcgccgc
     3841 tgacttcgga cagctgctcg atgccgtcgc tgtcctgcgc aaggagatcg cccaatgaac
     3901 ctgcaagcac ttaccgccaa agcgcgcgcc gttcgcggca atatcgtggc cgcagtttcc
     3961 accaaaggca gccgcaccaa atcgcccgtt tacgagcgtg acgaacaaat caaactgcgt
     4021 gaacgtatcc agcagacgca gcccgagtgg gtgttgttgt ggtgggatat ttccgtggtt
     4081 actggctggc gtacctctga cgtgtgcaac ctgcgttact cctgcatcga ctggacgaca
     4141 ggtaaagcaa ccatcaccgt ggccaagcag actaaggccg cagaagcgcg tgcaacccgc
     4201 aaaggtgttg agctggtacg caaggcccgt aaagacgcct gtaggctctc aggcgaccac
     4261 gtaggctata tgcagtggga tagcgcgacc gcagacgaga tcgccgcgca catgacggca
     4321 gaggagcagg aagcgtgctt tgagctggtc agccgtgccg atgttaagcg tgacaccaaa
     4381 caactccccc caggcatcct gaagcgcctc cgtgaacgtc aggagcgcaa cctgattgac
     4441 gatgatctcg tattctcccg ctcccagatt gaaagtaacc gttgcgccag tctggatggc
     4501 cctatcacgc gccagagtgt ctggcgtcgt ctacacactg tgtgtacatg gttcatgcgc
     4561 cacgttaacg caaagttgcg cctgagcgcc tacagcaccc gcaaaattgc cgcattcaac
     4621 atgatgcgcc gtggtggtga gcagggtctg ctgatcgcgt ctgagatgct cggacatagc
     4681 aacccggcag taactcgcac ataccttcag ctgggtagcc aggctggaga actccaggcg
     4741 gcaatggcaa tggaggtgct ggcgtgagta tctataacga ttttgtgcgt gtgaacttcc
     4801 ctgacgttca gggtgacgat ctgctgagct tatctggccg cgccgagtgc gctgctgact
     4861 caatactcaa cggcgtcgct gctgttggca aaatgatgtt ctatgccggt gatatagata
     4921 acagcgttta tgagccatca acggctgatt tcagtgatat cggcgggatg ttgatggagc
     4981 tgatgccact tgtccgggtg cttacggata ccgcagccaa tgcggaatgt cagcaccggc
     5041 agatggcaaa gcgtaagtaa ccgactaact actctgccgc ttcggtgatg gtaaacttga
     5101 gctgtaaaaa cggaacgccc cggagctggc aggctaccgg ggcgtaatac aaagcaatgc
     5161 gtcgacaggg gttattttag cgaaaatagc cgttaatctc cacctgttcg ccgctgtttg
     5221 aggttattta atggcgcgtg aattcttcat tgttgaccgc gacgtgttcc tgcgggcgtg
     5281 tgaacgcggc atgaatgcgg cagtgagcta tctggttatg gctcgcggta cccagggcaa
     5341 taacaggacg accaaatgga gcgccaacgc gatcgccgaa cgagccggaa tgaccaggcc
     5401 acgtgcaaca gctgcgatcg gagagctcat cgattccggc gttgttgagc tggtatctgg
     5461 cggcgcacga ccacattacc gactccactt accagacagt gacaacggtt acttatacct
     5521 tccaaacagc atcatcgatg ccgcagcaga tgaaaccccg ccaatagagc gcctgcggca
     5581 gacgcgcaat attacgaacc tgattgtatt tgtcttgctg tatggcattc aggatctgga
     5641 taactcaggc ggcctaccag ctgacatgtt tgatgacacc ccatcagaag cggaagttct
     5701 gtatgaaaac aaaactatgc gtttggaggt ggtgaccaag catacctata agctgagctg
     5761 ggcatggtat agcaggtcat cagtattttc cctcatcgag atggatttta acgacttcaa
     5821 ctcagcgttc gcagagctaa gggatatggg cctggtctcc gttaacgctg ctgtatttga
     5881 tcaccatgaa tacgatggtg agataattgg ctctacacat ggatatgatt acgttgaaga
     5941 gctggatgtc gctatacgcc gcaagcgaaa gaccatggct aaatggcctg atgggtatta
     6001 catgatcatt ccggcgcaca taaaaacggc ctgcataaaa tgcgttgggg ttatgcgtta
     6061 ccgtgcacaa accggcaaga cagcgcgctg ggtggcgcta tctacaaaga atggggccag
     6121 ggaactaaag ctgtataacg acgcattaag actggagctt gcccttgcta ttgagcgtga
     6181 agtaagaact gcttagtgct ttttttaacc aaatcgtaca catcaaggtt tatcaatgtt
     6241 aatcaaggta tatcaatgct tatcaatgat attcaaggag agtaaacaca tacctcagca
     6301 gagagaaaaa actctctgcc agcaatatca aactgcacag tgaaacccaa ggtcaaaatc
     6361 aaaagacaaa acccgctttg cagggtgaag ttcaaaggca aggtcaaaac caacacccag
     6421 cacagatttc aaatgcgacc cccagaaggg tgacatttca aattctatcc acgcaaatac
     6481 tcaaaggaca gaaatgtcct tagtggtggc aaccagctca attctgagcc cgttctccat
     6541 ccttcctcac tttggtcaca gggctccgcc attgccggaa gttaggtatt acgaaacttt
     6601 cgtagttcta tgtgacagaa tgccgagacg gtgaaggggt attttgatcc tggatcttcc
     6661 agggacgggc ggaacggggt aaggtaatgc ccagaaacga aaaaacccac gttctcaggc
     6721 gtgggcttta acgggtaaac gagttttctc aggacacgtt tacctggtaa ctgtgtcttt
     6781 tgcgggaacc agctaccgaa acgtactttc agtgtatccg cgttacgcgg tgcattcaag
     6841 ccacaaccgg gcgaatttgt gccagtttgc ctctcagcag cgttcccgca cccaagacca
     6901 gttaccgcat tggctaatgc cattggcggc gtacagccgt atctgcgcga cgtggagtcg
     6961 agtgattagt ccgcgtgtag atgcgttagc ctgggcgaga cggggagtct gattaacacg
     7021 cccggcgttg gagcgactac ctaaacagaa agccgatcat cagatagcgt gaggaatgag
     7081 aaaacgccac gctcccagca ggggaaaggc ggattgatga cggcaaaagt gcatggtggg
     7141 atgtcaggac gcaatcaggg gagcacggca aggaaacaag ccgaatctca aagctggcac
     7201 ggtttcgcta ctcttggctt gagctgtcac tttcgtgatg ttcgccagga gtagcggagc
     7261 tatggaaaaa gggcatcggg tgattgtgga ccatgcttat cgcctgcatg ttgtccgttt
     7321 gggcataggc cccaacgctg ttctgcaatg acagctggta gcggtactgg gtactggtta
     7381 gtccaggtac ccccatttct tcaggcgcgt cttcgtattc atcacgactc tgtgagtgag
     7441 ttctccgcct ctgcccgggc taacgccatc agcatttagg cgatcggcaa tttcgtgatt
     7501 gctggttatg gttctggcca actcgagaac ggcagcattg aatgtgcctg gtgcgattct
     7561 tggatcagca ccgttatcgc ctggtgtatg cacagtgtgt gtcttgggct cagccggttt
     7621 ctttactggc ttgctgctga tggacttcac cggcttaacc tcaatcgctg gcaatgctcg
     7681 ctcctggata gtctcgaact gctgtgcgat ggattccagc cgcttcactg ccgcgttcag
     7741 cgagatttct ggcgtagcgc cgccggctaa ctttctggcg caggcatcaa tcacccaggc
     7801 tgcgaaactc agatcaccct gtgcctctgc gatatcctca agtagctgat gtggaaacct
     7861 gatgttcttc actgctgact gcccattcac gttcttactc gccatcaatc ctcctgtatg
     7921 cacaatccac cacacaccgt gtatgcacag cagtgtatac cactaattcg tggttttcac
     7981 acacatgtac acacagtgac actgtcacgt atgcacacct gcacacacac cgcatgtcac
     8041 acactgtata cacatcacag attcagttaa ttacctcctt ttatacaatt aaaagtgatt
     8101 gatattgtat atttaaccag ttttatagct ataatgttca ttacttaacc gcgctaggcg
     8161 cattgcataa ggtgcaaaca tgctgaaact ccgcattacc tccgacgatt tccaatccct
     8221 cgataaagcc ctgcaaccct tctacgttga acagtctgat ggttacgcgc tgaacgtcga
     8281 acaggacgac accgccgccg ctggccttaa agcgaagaac gccgagctgc tgactaagtt
     8341 agccggggag aaagagcgcc ggattactgc tgaggcgcag ttaaaagtgg ttaacgaaac
     8401 cgtcgccggg cacgaccaga aaatcaacca gatcgccgga gagcgtgaca gctaccgcgc
     8461 cgggctggag aaatccctca ttgacgagcc gctgacagcg ttcatgcgct ccaacttcat
     8521 tgatggatca gacgattttg tggtgccggt gctgaagaaa ttcttccagc ttcaccgcaa
//
