LOCUS       NZ_CP056893             3696 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00053 plasmid pRHBSTW-00053_6, complete
            sequence.
ACCESSION   NZ_CP056893
VERSION     NZ_CP056893.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148462
            Assembly: GCF_013796805.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00053
  ORGANISM  Citrobacter sp. RHBSTW-00053
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00053
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.8393625726566x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:10:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,477
            CDSs (total)                      :: 5,361
            Genes (coding)                    :: 5,182
            CDSs (with protein)               :: 5,182
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 48 of 179
            Pseudo Genes (incomplete)         :: 138 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 35 of 179
            Pseudo Genes (short protein)      :: 1 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056893.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Citrobacter sp. RHBSTW-00053"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00053"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742632"
                     /plasmid="pRHBSTW-00053_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            147..632
                     /locus_tag="HV045_RS27145"
                     /old_locus_tag="HV045_27170"
     CDS             147..632
                     /locus_tag="HV045_RS27145"
                     /old_locus_tag="HV045_27170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064181052.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIQAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGSYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            636..848
                     /locus_tag="HV045_RS27150"
                     /old_locus_tag="HV045_27175"
     CDS             636..848
                     /locus_tag="HV045_RS27150"
                     /old_locus_tag="HV045_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1390..1857
                     /locus_tag="HV045_RS27155"
                     /old_locus_tag="HV045_27180"
     CDS             1390..1857
                     /locus_tag="HV045_RS27155"
                     /old_locus_tag="HV045_27180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071047206.1"
                     /translation="MSKYYPELFIISMFLISVALQVWIVARAGEKKRIYAFIFSIIASV
                     LVVGLIKAPFSSIVAFAVLFLMKDKENESIGKKLFFDFLGFFFGSVGIVFYISIVVFPV
                     GGLYWLWTAIQLKSFSMFLLGVIPFLWIVTSPIGLYSLITDVPDWIYDFFG"
                     /name="hypothetical protein"
     gene            complement(2123..2206)
                     /locus_tag="HV045_RS27685"
     CDS             complement(2123..2206)
                     /locus_tag="HV045_RS27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072050248.1"
                     /translation="MHEPPVQSDRCALSGNYLLESNPDRHD"
                     /name="replication initiation protein"
     gene            2824..3165
                     /locus_tag="HV045_RS27160"
                     /old_locus_tag="HV045_27185"
     CDS             2824..3165
                     /locus_tag="HV045_RS27160"
                     /old_locus_tag="HV045_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_074195894.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPAR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2558..2731
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.714"
                     /coverage="100.0"
                     /evalue="2.67e-56"
     Typing          complement(2079..2591)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3..964
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3129..3694
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.005"
                     /coverage="95.0"
                     /evalue="2.56e-107"
ORIGIN
        1 agcctcatcc ggggaactca aaacgcgcga agacgtcaca ggggcgctca cagctgcagg
       61 ttttgaggtg gtccgcacta caaaaaacag tatcagcatt gccgaccctg acggggggcg
      121 aaacatccga ctgaagggag ctatttatga gcagtctttt agcgctggcg aaggacttgg
      181 agcagcaatc gaaagcgcag cagcagagta ccggcgagat gctgaaagcc gcattcagcg
      241 agcacgagaa gtctgtaaaa gcggaactga gcgcaagcgc gaagagaatc agcgacgcca
      301 tcagcgccca cgagcagggt atgaccgcgg cgatgcagtc gaaccggctg agcgtgctgc
      361 gtatggtcgg ccggacgtgg ctgaccatca ccctggtctc ggtactgctg atcgccacga
      421 gcgggagcat tctgtggtgg caggggcagc agatcaccgg gaattaccag actatccagg
      481 cgcaggagcg gacgcaggcc atgctgtcgg agaagaacca cggcgtgcag ctgtcgctct
      541 gcggggagaa gaaactgtcc tgcgtcaaag tgaacccgaa agcgggatct tacggggagg
      601 aagggaactg gatggtactg gagaggaaat agcccatgac ggaactggaa aaacagttgc
      661 tgaccgcatt agagcagcta catcaggact actcgcaaag gctggacgag tgggagagcg
      721 ccttcgcgga atggcagaag atgtctggtc ttatgcagcg ggagaacgcg gcgctgaacg
      781 agcgcgtgac gcgcttgagt cagcaggtgg cgaacttgag caggcagtta cagcgtttga
      841 gccagtagtg cagcggcatg aaatggcggt ggccgccgaa cgggcatacg agcagcgaca
      901 gcacgaaaaa gagctggcgg aaacccgttc ccgtcagcgg agctataacg gtccgacgct
      961 gggctgaaca gtatcatccg gtgacaggcc gcacgaagaa gggctcataa atttcccgct
     1021 aaatacggtc gtgatgaaaa catcacggcg tatacaacgg agggattatg agactgttta
     1081 acgtgattga gacggtgctg aactgcctgt acatggcgct ggagatctgg gactggctgg
     1141 agaagcattt tcctgttttt aaggctttca tcccgattct ggcctgaaat cagaccgtat
     1201 gcatctggct gcatgaccat gcagccgggt aaaatttatc tgtcacgaac ggggcggtgt
     1261 atcgggtggt ttgttgccgg ttttacctga ctgccgcccc gtttgtggcg aacctgtccg
     1321 gagcggtgcg ggcaagagat gttatggtaa ataaagctac atacatgccg agtaaaatgg
     1381 agaattcaat tgagcaaata ctatccagag ctttttatta tatccatgtt cttaataagc
     1441 gttgctttgc aagtttggat tgttgctagg gctggcgaaa aaaaaagaat ttatgctttt
     1501 attttttcga taatagcttc tgttttagtc gttggtttaa ttaaagcccc attttctagc
     1561 attgttgcat tcgctgtgct ttttcttatg aaagacaagg aaaatgaaag cattggaaaa
     1621 aaacttttct tcgatttttt agggtttttt tttggttctg tagggatagt tttttatatt
     1681 tctatcgttg ttttccccgt tggaggattg tattggttat ggacagcaat acaattaaaa
     1741 agcttctcta tgtttttatt aggcgtgatt ccttttctat ggattgtgac atctccaatt
     1801 ggattgtata gcttgattac ggatgtccct gattggatat acgatttctt tggttagttg
     1861 aagtttgcgt atagcgaagg atcttcttgt gattcattct ttagagtcgt aatctcttgc
     1921 cctgtaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1981 cttctgaacc gtggtaacag tcttgtgcga gacgtgtcac ccaatctgtc cttccagtgt
     2041 agccgtagta agggcaccac ttcaagaact ctcacaatat ctctcgcaca tcctgtttac
     2101 cagtggccgt tgccaatggc gattagtcgt gtctatccgg gttggactca agaagatagt
     2161 taccggacaa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     2221 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagataaacg cggccataac
     2281 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagctacca
     2341 gggggaaacg cctgggatct ttatagtcct gtcgggtttc gccgccactg atttgagcgt
     2401 cagattttgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccgcggcc
     2461 tcatcgcttc cctgttaagt ttcttcctgg aaatttccgc cccattcgta agccggtacc
     2521 gctcgccgca gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatctc
     2581 ctgcatcgca tcttctgctg acgcgccggc gttacctatt ttctcctgct acatgaagca
     2641 ctttcctgat aaccgcactc atgccaacat aataagccag tatacactcc gctagcgcta
     2701 cgtgactggt tcagggctgc gccccgaaac ccgctaaaag cgactgacgc gcctgcggct
     2761 tgtccaacac cgtgccgacc gggaaacatt tcccgcccgt cccggtgtta tcaggaggct
     2821 actctggcag acaaacgcaa caagatgctc acgatgtggg tgacggaaga cgaacaccgg
     2881 cggctgctcg agcggtgcga cggcaggcag ctggcggcgt ggatgcgtca gacctgcctc
     2941 gatgagaagc cggcgcgtgc cggcagactg ccgtcaatat cgccggcgct gctgcgccag
     3001 cttgccggta tgggtaacaa cctgaaccag atcgcccgga aagtgaatgc cgttggtgcc
     3061 ggtcatgatc gggtgcagat tgtggccgcc ctgatggcaa tagacgcggg actggagcgg
     3121 ctgcggcatg cggtgctgga aaaagggggg gatgatgatc gttaaatttc atccccgggg
     3181 gcgcggtggt ggtgccggtc cggtggatta cctgctggga aaagaccggc agcgcgaggg
     3241 ggcgaccgtg ctgcagggaa aaccggagga agtccgggag cttatcgatg cctcgccgta
     3301 tgcgaaaaag tacacctccg gcgtgctgtc gttcgcggag gctgaactgc cgcccgggca
     3361 gcgggagcag atcatggcca gctttgagcg ggttctgatg cccgggctcg ataaagacca
     3421 gtacagcatt ctgtgggtgg agcacaccga caaggggcgg ctggagctga atttcctgat
     3481 ccccaacact gaactgctga ccgggaaacg cctgcagccg tactacgatc gcgccgaccg
     3541 tccgcgcatc gatgcctggc agaccgtggt gaacggcaga ctggggctgc atgatccaaa
     3601 cgcccctgag aaccggcggt tactggtcac gccgtccgca ctgccggaaa cgaagctgga
     3661 agccgctcag gcgattacac gggggttgat ggcctt
//
