LOCUS       NZ_CP056891             6382 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00053 plasmid pRHBSTW-00053_4, complete
            sequence.
ACCESSION   NZ_CP056891
VERSION     NZ_CP056891.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148462
            Assembly: GCF_013796805.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00053
  ORGANISM  Citrobacter sp. RHBSTW-00053
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00053
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.8393625726566x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:10:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,477
            CDSs (total)                      :: 5,361
            Genes (coding)                    :: 5,182
            CDSs (with protein)               :: 5,182
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 48 of 179
            Pseudo Genes (incomplete)         :: 138 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 35 of 179
            Pseudo Genes (short protein)      :: 1 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056891.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Citrobacter sp. RHBSTW-00053"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00053"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742632"
                     /plasmid="pRHBSTW-00053_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            138..962
                     /locus_tag="HV045_RS27090"
                     /old_locus_tag="HV045_27095"
     CDS             138..962
                     /locus_tag="HV045_RS27090"
                     /old_locus_tag="HV045_27095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_191233490.1"
                     /translation="MGHEFTVCMVVTRNDSAEHFNVALLSLTCQSMKPSEILIVDNGGL
                     TDRHYEIIASIDDELSIRLVRIANLSPVGQARNIALHAARYKVIAVADPDDVNEPDRFM
                     KLVPELNDSVRMVGAWVREFSHEKSDRQIERRVPCDFVDIIRYSRFRSPVNNPTICYFK
                     NDALGVGGYNTSLRFGEDYDLVMKFIRSGKIIHNIPIVTVSFRTGDAKILHKKRSGLFL
                     LKEEIKLHRIFLVNGDISLLDFFIVIAIKVLVRLLPCSLFEMFYTKVLRKHE"
                     /name="glycosyltransferase"
     gene            955..2334
                     /locus_tag="HV045_RS27095"
                     /old_locus_tag="HV045_27100"
     CDS             955..2334
                     /locus_tag="HV045_RS27095"
                     /old_locus_tag="HV045_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_200769345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_191233491.1"
                     /translation="MSDYVIDIVEGVLYILVSVMTWYLFILSSYSIFLSLFGFSKNKKD
                     YPDCPPEASFLILVAAHNEEAVIGSTLINLKNIQYDKKLFDIVVVNDNSTDRTGLICDS
                     HEVKHVDTCEGEFEREGVGKPAGIQYALRKLGFETVKEKYDLVMVLDADNFVDANILTE
                     LNSQWISKGKPEAIQAYLDCKNSTSLLSFGYCTSYWMMNRFFQLSKYRLGLPNAIGGTG
                     FVVSSNFLINTGGFCFKSLTEDIELEIEIVRKHGRVLWNHNVRVYDEKPDNLRVSLKQR
                     YRWSKGHWYVAFTNALNLLKLTFIERKWKYVDQLLYLFSMGRALQVSIIFINLFMLSLL
                     KENYRPEIVNISTTMTDLAVTNIRSSDSVIAIFSSIDWISVITHLNIVTLVSICYGMLI
                     LPIYGAWMDKGIFLNPFKVFFSGLYFGLSFVLVQFLALFRWKKQHKWVVTPHNKTKEEH
                     K"
                     /name="glycosyltransferase family 2 protein"
     gene            2331..3464
                     /gene="wecB"
                     /locus_tag="HV045_RS27100"
                     /old_locus_tag="HV045_27105"
     CDS             2331..3464
                     /gene="wecB"
                     /locus_tag="HV045_RS27100"
                     /old_locus_tag="HV045_27105"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048266654.1"
                     /GO_function="GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="WP_145509229.1"
                     /translation="MSKVLFVFGTRPEAIKMAPLVIEFKKNPDIEVKVCVTGQHREMLD
                     QVLEFFEIEPDYDLNIMKQQQSLGSITCSILTLLDEILVSFMPAHIFVHGDTTTTFAAS
                     LAAFYQNIKVWHIEAGLRTWNMQSPFPEEGNRQLTGKLASFHVAPTLQAKDNLLRENVS
                     EKNIIVTGNTVIDALLVGIKKITGPTGNTREVINLKNQLNLDKKIILVTLHRRENQGGL
                     LRTICDDIKQLAIEHDDIEIVFPVHMSPRIREVVNEKLSGVVNIKLVEPLAYPGFIWLM
                     NNAHFILSDSGGVQEEAPSLKKPVLVARDTTERPEVIENGAAMLVDPRIPNNIYFSCKK
                     LLSDERFYEKMSQAGNPFGDGTASKKILDYFVSLDDF"
                     /name="wecB"
     gene            complement(3762..3926)
                     /locus_tag="HV045_RS27665"
     CDS             complement(3762..3926)
                     /locus_tag="HV045_RS27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_145509230.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNWNYTREIL
                     REVLKLSPD"
                     /name="replication initiation protein"
     gene            4568..4891
                     /locus_tag="HV045_RS27105"
                     /old_locus_tag="HV045_27110"
     CDS             4568..4891
                     /locus_tag="HV045_RS27105"
                     /old_locus_tag="HV045_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_186371729.1"
                     /translation="MLTMWVTEDEHRRLLERCNGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQTARKVNSGAWSGHDRVQVVATLMAIDAGLERLRHAVLEKGRS
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5100..>5534
                     /locus_tag="HV045_RS27670"
                     /pseudo=""
     CDS             5100..>5534
                     /locus_tag="HV045_RS27670"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5587..6099
                     /locus_tag="HV045_RS27265"
                     /old_locus_tag="HV045_27120"
     CDS             5587..6099
                     /locus_tag="HV045_RS27265"
                     /old_locus_tag="HV045_27120"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_326928681.1"
                     /translation="MATDFRQKSSEQASDTEQLLKQEFDRLGTCVSEALSLSGQKISDD
                     IAARNRELSEALRHHSSGMTEALQLHRESVMHLATRRWLSVLMTSLLLAGTCGGVLWLQ
                     SWKIASNLEDIAAQNATLEKLNAKTWGVTYLEDRNGRFLVLPKGMKAEAGWTVDNGKRN
                     AVKLVKE"
                     /name="MbeB family mobilization protein"
     gene            6106..6321
                     /locus_tag="HV045_RS27270"
                     /old_locus_tag="HV045_27125"
     CDS             6106..6321
                     /locus_tag="HV045_RS27270"
                     /old_locus_tag="HV045_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_220137637.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFGEWRTMCGLMQRDSE
                     RLSGQVMRLSQQVDRLSGQLSRLSRQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4282..4458
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.333"
                     /coverage="83.0"
                     /evalue="2.08e-53"
     Typing          complement(3815..4299)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.796"
                     /coverage="93.0"
                     /evalue="1.2600000000000002e-173"
     Typing          4855..5648
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.755"
                     /coverage="95.0"
                     /evalue="4e-134"
ORIGIN
        1 ggaactgaca aaagagcgaa aatatcctgg gccatcgctt gaactgtagt tgacacatct
       61 tagtacggag ctttcaggta aaaatcaaga ttttttacct gtctgcatgg ctatgcagta
      121 caggagatat aggaaacatg gggcatgagt ttactgtttg catggtagtg acacggaacg
      181 attcagctga acatttcaat gtagctctgt tatctcttac ctgtcagtcc atgaaaccat
      241 cggagatatt gattgttgat aatggtggat taacagatcg acattatgaa atcattgcca
      301 gtatagacga tgaactaagc attcgtctgg tgagaatagc aaatctaagc ccggtagggc
      361 aggcgagaaa catcgctttg catgctgcaa ggtacaaagt tatagctgtg gctgatccag
      421 atgatgttaa tgagcctgat cgttttatga agcttgtccc tgagctaaac gatagcgtaa
      481 gaatggtggg ggcctgggtg cgagaattca gccacgagaa aagcgataga caaattgagc
      541 gtagagttcc ttgtgatttt gtagacatta tccgctattc acgatttcga tcacctgtta
      601 ataatcccac tatctgttac ttcaaaaatg atgcgctggg tgtggggggg tataacacgt
      661 ctttacgttt tggggaggac tatgatttag tgatgaagtt tatccggagt gggaaaatta
      721 tacataatat tccgattgtt acggtgagct tcagaaccgg tgacgctaaa atacttcata
      781 aaaaaagaag tgggctcttt ttactcaagg aagaaataaa actgcatcga atctttttgg
      841 taaatggtga tatttccctg ttggatttct ttatagttat agcaataaag gtgctggtca
      901 ggttgttacc atgtagttta tttgaaatgt tttatactaa ggtgctaaga aagcatgagt
      961 gattatgtaa ttgatattgt agagggtgtt ctatatatcc tcgtatctgt tatgacatgg
     1021 tatttgttta tcttgtcttc ctattccatt tttctgagtt tatttggatt ttctaaaaat
     1081 aaaaaagact atccagactg tcctcctgaa gccagctttc taattcttgt tgctgcgcat
     1141 aatgaagaag ctgttattgg ctcaacactc attaatctta aaaatattca atatgataaa
     1201 aaactgtttg atatagtagt ggttaatgat aattccactg atcggacagg acttatctgc
     1261 gatagtcatg aagtaaagca cgtggacact tgcgaaggag aatttgaacg tgaaggagtg
     1321 ggcaagccag caggtattca gtacgctctg cgcaagcttg gatttgaaac tgttaaagaa
     1381 aagtatgatt tggtcatggt gttggatgct gacaattttg ttgatgctaa tatccttacc
     1441 gaactgaatt ctcaatggat atcaaagggc aaaccggagg ctatacaggc gtatctggat
     1501 tgtaaaaact ctacttctct tctctctttt ggttactgta catcctactg gatgatgaat
     1561 cgatttttcc aattatcaaa atatcgctta ggcctgccta atgcaattgg cggaacagga
     1621 tttgtagtat ccagtaactt tctgataaat actggaggct tttgttttaa atctctgacc
     1681 gaggatattg agctggaaat tgaaattgtg cgcaagcatg gtcgtgtgct gtggaatcac
     1741 aatgtacgcg tttatgatga aaagccagac aatctacgag taagcctcaa acaacgctat
     1801 cgctggtcaa aggggcactg gtatgtagct tttactaacg ctcttaactt gttgaagtta
     1861 acctttattg aacgcaagtg gaaatacgtc gatcagttgt tatatctgtt ttctatgggg
     1921 cgtgctttac aggttagtat tattttcatt aatcttttta tgttgagttt acttaaagaa
     1981 aattaccgtc cggagattgt aaatatttct acgacgatga cagatcttgc tgtgaccaac
     2041 attagatctt ctgacagtgt tattgcgata tttagctcaa tagattggat tagcgttatt
     2101 actcatctta atattgtcac attagtatcc atctgttatg ggatgctgat tttgcctatt
     2161 tacggtgcat ggatggacaa aggtattttt cttaatccct ttaaggtatt tttttccggt
     2221 ttgtattttg gattgtcgtt tgtgcttgtt caatttttag ctcttttccg ttggaaaaaa
     2281 cagcataaat gggtagtcac gccgcataat aaaacaaagg aagagcacaa atgagcaagg
     2341 ttttatttgt ttttggtacg cgtcctgagg ccataaagat ggctccgctt gtcatagaat
     2401 ttaagaagaa tccagatatc gaagtgaaag tctgcgttac gggacagcat cgagaaatgc
     2461 ttgatcaggt gctggaattt tttgaaattg aaccagacta cgatttaaat atcatgaagc
     2521 agcaacagag ccttggttcc attacatgtt ccattttaac gctcctagat gagattctgg
     2581 tgtcgttcat gcctgctcat atatttgtcc atggcgatac cactactaca tttgcagcct
     2641 ctttggctgc cttctaccaa aacattaagg tatggcatat tgaggctggt ttgagaactt
     2701 ggaatatgca gtcacctttc cctgaagagg gtaaccgaca actcacaggt aagctcgctt
     2761 cttttcatgt agcacctact ctccaagcta aagacaatct ccttcgagaa aacgtgagtg
     2821 aaaaaaatat cattgtcact ggtaatacgg tcattgatgc actgttagtg ggtataaaaa
     2881 aaataacagg tcccactggt aacactcgag aagttataaa tttgaaaaat cagttaaatc
     2941 tggataagaa aattatctta gttacactac accgacgtga gaatcaggga gggttactgc
     3001 gaactatttg tgatgatatt aaacaattgg caatagagca tgatgatatt gaaattgttt
     3061 ttcctgttca tatgagtccc cgtatccggg aagtggttaa tgagaaactg tcaggagtgg
     3121 tcaatatcaa gttagttgag ccattggcat atccgggatt tatatggctt atgaataatg
     3181 ctcatttcat tctgagtgat agcggcggcg tacaagaaga agctcccagc ttaaaaaaac
     3241 ctgtactggt agccagagac actacagaaa ggccggaagt cattgagaat ggtgcagcta
     3301 tgctggttga tccacgtatt cccaataaca tctattttag ttgcaaaaaa ctcctttcag
     3361 atgagaggtt ttatgaaaaa atgagtcagg cggggaatcc attcggcgac ggaacagcga
     3421 gcaaaaaaat cctcgattat tttgtttcct tggatgattt ttaaatgatc gtcacccaac
     3481 tggtcgtttg tgcgacagcg cccgccgtta ctggtcgcag gaacatccag gtttccgccg
     3541 tagaatccca ttaactgacg ggcatcaaag ttattcaata aaaattaaaa ggatcttctt
     3601 gagatccttt ttttctacgg gtaatctctt gctctgtaaa cgaaaaaacc cgcctgatgg
     3661 cgggttggtt cgtccagtta gaagtcttcg acctcttcta actggagtgg tagctgggta
     3721 tacgagaccg cagataccaa aactgttctt ccagtgtagc ctcaatcagg cgataacttc
     3781 aagacctctc tcaatatctc tctcgtataa ttccagttgc cagtggctgc tgccagaggc
     3841 gctttgtcgt gtctttccgg attggactca agacgatagt taccggataa ggcgcagcag
     3901 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     3961 ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga caccagtaaa
     4021 ccgaaaggca ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct
     4081 ttatagtcct gtcgggtttc gccgccactg atttgagcgt cagatttcgt gatgcttgtc
     4141 aggggggcgg agcctatgga aaaacggcaa cgccgcatcc ttgttccccg tccttcctga
     4201 tttgagcacc atctatttat cccgccattc gtgagcccgt aacgcccgcc gcagtctcac
     4261 ggccggagcg tagcgaccga gtgagcgagg aggcgtcatt tcatcctcac ctgcatattg
     4321 cacttacgcc ctgcagccac attttccctt tccacatgaa gcactttcct gaattcctac
     4381 tttgatttaa catattaagc cagtatacac tccgctagtg ctacgtgact ggttcagggc
     4441 tgcgccccga aacccgctta aagctactga cgcgcctgcg gcttgtccaa caccgcgccg
     4501 accgggaaag atttcccgcc cgtcccggtg taaccaggag gccggattgg cagataaacg
     4561 cagtaaaatg ctcacgatgt gggtgaccga agatgagcac cgccgcctgc tggaacgctg
     4621 taacggtaaa cagctcgcgg cctggatgcg gcagacgtgc ctggacgaga agcccgcccg
     4681 cgccggcaaa cttccctcaa tctcgccggc gctgcttcgt cagcttgccg ggatggggaa
     4741 caacctgaac cagacagccc gcaaagtgaa cagcggggcg tggtccggtc atgatcgggt
     4801 gcaggtggtg gccacgctga tggccattga tgccggactt gagcgactgc gccatgcggt
     4861 gctggaaaaa gggcgttccg atgatagtta aatttcacgg cagaggacgc ggcggcggtt
     4921 ccggcccggt ggattacctg ctggggaagg agcgtaaccg ggagggcgcc agcgtcctcc
     4981 aggggaagcc ggaagaggtg cgggagctta tcgacgcttc gctgtatgcg aagaaataca
     5041 cgtccggggt actgtccttc gcggaggcag atttaccgcc gggccagcgt gagaagctga
     5101 tggagagctt cgagcgggtt ctgatgcccg gactcgataa agaccagtac agcgtcctgt
     5161 gggtggagca ccgggacaag gggcggctgg agctgaattt ccttatcccg aataccgaac
     5221 tgctgaccgg aaagcggctg caaccgtact acgaccgtgc cgaccgtccg cgcatcgacg
     5281 cgtggcagac aatcgtgaac gcacgtctga gcctgcatga cccggacgcg ccggagaacc
     5341 gccggacgct ggtcacgccg aataccctgc caaaaacgaa gcaggaggca tctgaggcta
     5401 ttaccgacgg tctggtacgt ctttgtgtgg ccggagaact gaaaacgcgt caggacgtga
     5461 tacaggcgct cacagcgtca ggatttgacg tggtgcggac cacgaagacc agcatcagca
     5521 tcgcggaccc ggaggggggc tgcaatctca gactcagggg agcaatctat gagcagtctt
     5581 tcgaaaatgg cgacggactt caggcagaaa tcgagcgagc aggcgagcga taccgagcaa
     5641 ctgctgaagc aagagttcga caggctcggg acgtgtgtca gcgaggcact gagcttaagc
     5701 gggcagaaaa tcagcgacga tatagccgcc cggaacaggg agctgagcga ggccctgaga
     5761 caccacagca gcggcatgac agaggctttg cagttacacc gggagagcgt gatgcacctg
     5821 gcaacgcgcc ggtggctgtc ggtactcatg acctcgctgc tgctggccgg gacgtgcggg
     5881 ggcgtgctgt ggctccagag ctggaaaata gcgtcgaacc tggaagacat cgcggcgcag
     5941 aacgccacgc tggagaagct gaacgcgaag acgtggggcg tgacgtatct ggaggacagg
     6001 aacggacgtt ttcttgtgct gccgaagggt atgaaagccg aagcgggctg gacggtggac
     6061 aacgggaagc ggaacgcggt gaagctggtg aaggagtaac ggaacatgac agagctggaa
     6121 aagcagttgc tgagcgcatt agagcagcta cagcaggact actcgcaaag gctggacgaa
     6181 tgggagagcg ccttcgggga atggcggacg atgtgtggtc ttatgcaacg ggacagcgag
     6241 cggctgagcg ggcaggtcat gcgcttgagt cagcaggtgg atcgcttgag cgggcaactg
     6301 agtcgtttga gccgtcagtg aagcggcacg agtacgaggt ggcggctgca cgtgcgcagg
     6361 tggtgcatga gcagcaccag aa
//
