LOCUS       NZ_CP056890            10160 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00053 plasmid pRHBSTW-00053_3, complete
            sequence.
ACCESSION   NZ_CP056890
VERSION     NZ_CP056890.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148462
            Assembly: GCF_013796805.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00053
  ORGANISM  Citrobacter sp. RHBSTW-00053
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00053
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.8393625726566x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:10:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,477
            CDSs (total)                      :: 5,361
            Genes (coding)                    :: 5,182
            CDSs (with protein)               :: 5,182
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 48 of 179
            Pseudo Genes (incomplete)         :: 138 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 35 of 179
            Pseudo Genes (short protein)      :: 1 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056890.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Citrobacter sp. RHBSTW-00053"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00053"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742632"
                     /plasmid="pRHBSTW-00053_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(9947..10160,1..812))
                     /locus_tag="HV045_RS27030"
                     /old_locus_tag="HV045_27040"
     CDS             complement(join(9947..10160,1..812))
                     /locus_tag="HV045_RS27030"
                     /old_locus_tag="HV045_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="WP_191233487.1"
                     /translation="MKKYLLLGLLINNAGFAAGINAIEQGKNATATGVSSIAIGTNAKA
                     EELVAVSIGANSEAKGKSSVAHGANAQALGGVDVAVGSRALASGGSGVALGSGAKSTGN
                     YSTALGAGASSSGNGATAIGVQSEAKGNVSLAFGRGAIANNESSVALGSGSQTSDVVQT
                     KNATVNGITYEGFAGSEATSSVSVGREGAERVINNVGAGRVSDTSTDAVNGSQLYSVAH
                     QSSDNAKSIQKTNRIVENNTKRISSIENKINDNSKHINDVENKMKRGFASQAALNGLFQ
                     PYGVGKFNLTAAVGGYNSDTAIAVGSGYRFNEDTAFKSGIATNTGNFEGVTYNAGINIE
                     W"
                     /name="YadA-like family protein"
     gene            complement(1027..2189)
                     /locus_tag="HV045_RS27035"
                     /old_locus_tag="HV045_27045"
     CDS             complement(join(1027..1928,1928..2189))
                     /locus_tag="HV045_RS27035"
                     /old_locus_tag="HV045_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="WP_191233488.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQ
                     LYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKYVF
                     IEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRY
                     GAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDF
                     YASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALW
                     RRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLCGSMSAKGCCYDNACVESFFHSLKVEC
                     IHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
                     /name="IS3-like element IS3 family transposase"
     gene            complement(2288..3244)
                     /locus_tag="HV045_RS27040"
                     /old_locus_tag="HV045_27050"
     CDS             complement(2288..3244)
                     /locus_tag="HV045_RS27040"
                     /old_locus_tag="HV045_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094396512.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYASFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDDFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKDPISKKEDKSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            complement(3840..4307)
                     /gene="traD"
                     /locus_tag="HV045_RS27045"
                     /old_locus_tag="HV045_27055"
     CDS             complement(3840..4307)
                     /gene="traD"
                     /locus_tag="HV045_RS27045"
                     /old_locus_tag="HV045_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_094396501.1"
                     /translation="MNKSEKQEQWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            4471..5568
                     /gene="mobA"
                     /locus_tag="HV045_RS27050"
                     /old_locus_tag="HV045_27060"
     CDS             4471..5568
                     /gene="mobA"
                     /locus_tag="HV045_RS27050"
                     /old_locus_tag="HV045_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_191233489.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKESEKLNARVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            5581..6165
                     /locus_tag="HV045_RS27055"
                     /old_locus_tag="HV045_27065"
     CDS             5581..6165
                     /locus_tag="HV045_RS27055"
                     /old_locus_tag="HV045_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040113348.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            6491..6859
                     /locus_tag="HV045_RS27060"
                     /old_locus_tag="HV045_27070"
     CDS             6491..6859
                     /locus_tag="HV045_RS27060"
                     /old_locus_tag="HV045_27070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191233483.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLFVDFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(7043..7231)
                     /locus_tag="HV045_RS27065"
                     /old_locus_tag="HV045_27075"
     CDS             complement(7043..7231)
                     /locus_tag="HV045_RS27065"
                     /old_locus_tag="HV045_27075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191233484.1"
                     /translation="MGRIVELKKTLQKLAQSRTWEKDFAEAMLLVQRGATIAISISVAV
                     VRVRLEKGNYPTLSVLS"
                     /name="hypothetical protein"
     gene            complement(7452..7679)
                     /locus_tag="HV045_RS27070"
                     /old_locus_tag="HV045_27080"
     CDS             complement(7452..7679)
                     /locus_tag="HV045_RS27070"
                     /old_locus_tag="HV045_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_191233485.1"
                     /translation="MTKQESAALNMAKFIRSQSLLLLEKLDVLDLDTEATECERLHEQA
                     EALFKALERRFEFDDIMDTHGTAYSELSKK"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8967..9191
                     /locus_tag="HV045_RS27075"
                     /old_locus_tag="HV045_27085"
     CDS             8967..9191
                     /locus_tag="HV045_RS27075"
                     /old_locus_tag="HV045_27085"
                     /inference="COORDINATES: protein motif:HMM:NF039402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="WP_020956904.1"
                     /translation="MNKNMTKAIRLNDEDFNLFESYANAKGITFSELCKSAVREKIEDE
                     LDLQCANESYADYLSDKTTISHDELFKSM"
                     /name="type II toxin-antitoxin system RelB family
                     antitoxin"
     gene            9203..9478
                     /locus_tag="HV045_RS27080"
                     /old_locus_tag="HV045_27090"
     CDS             9203..9478
                     /locus_tag="HV045_RS27080"
                     /old_locus_tag="HV045_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524350.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_191233486.1"
                     /translation="MTRYRTEYTKRFIKEFKKIDTVHQRVIQKYIEANINNALNPRLTG
                     KILKGDKAEFIRWRIGDYRLIATIEDDKLLVIALSVGHRKDVYKKF"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2289..3244)
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.478"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4472..5565
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.082"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaaaccctc tcttcatttt attttcaaca tcattaatat gcttgctatt atcatttatt
       61 ttattttcaa ttgagctaat acgtttggta ttattttcca ctattctatt tgttttctga
      121 atacttttag cgttatcact tgattgatgt gcgacagagt ataactgaga cccatttact
      181 gcatctgtac tagtatcaga aacacgccca gcaccaacat tgttaataac tctctcagcc
      241 ccttccctgc caacggaaac cgatgatgta gcttcagagc cagcaaaccc ctcataggtt
      301 ataccattga cggttgcatt ttttgtttga acgacatcag atgtttgaga accactcccg
      361 agcgctacag atgattcatt attagctata gctcctcgcc caaaggctaa agatacatta
      421 ccttttgctt ctgattgtac accgatagca gtagccccat tgccactaga agaagctcca
      481 gcaccaagag ctgtagaata attacctgta gatttagcac cgctacctaa tgccacacca
      541 ctaccacctg aagctaaagc tcgcgaccct acagcaacat caacacctcc taatgcttga
      601 gcattagcac catgagcgac tgaactttta ccttttgctt ctgaattagc accaatagaa
      661 acagcaacca gctcctcagc ttttgcattt gttccgatag caatggatga cacccctgtg
      721 gccgtagcat tttttccctg ttcgatagca tttataccag cagcaaatcc ggcattatta
      781 atcaacaacc caagtaaaag atattttttc atttgtgttt acctatttaa ttttagattt
      841 cagtgagtta tgttttatgg gaagaatctg acatgatttc cccatcaaac agtaagtcgt
      901 gtcaattcct agagttaaca catttctgat catgactcac acgttacgtt cagtcggtag
      961 ccatctgtgc aaaaaatgcg cttagcgtag gataattgat cctacccacg taatatggac
     1021 acaggcctaa gcgaggttct ggttttcaaa ttgttccgga ctgaggccgc cacaccaact
     1081 gtgccgccgc caccgattgt aatcacattc gatataatta aacaccgttg cccgcattat
     1141 ttcccggctg ataaagtgtt ctccatggat acattccact ttcagcgaat gaaagaagct
     1201 ttccacgcag gcattatcgt agcagcaacc ttttgcgctc atacttccac acagattatg
     1261 ccgcttcagt tgcgcctgat aatctgctga acagtactgg cctccacggt ccgtgtgaac
     1321 gataacgttc cggggcctct tacgccgcca cagcgccatc tgcagggcat cgcaggccag
     1381 ttgcgccgtc atgcgtggcg acattgacca gccaataacg gcacgtgacc acaggtcaat
     1441 gaccactgcc agatacagcc agccttcatc tgtacgtaag tacgtgatgt ctcctgccca
     1501 cttctggttc gggccactgg cgtaaaaatc ctgctccaac agattttctg acacaggcag
     1561 gccgtgtgcg cggtagctga ccgggctgaa cttccgggag gcctttgccc tcagtccctg
     1621 acggcgcagg cttgccgcca cggtttttac gttaaagggg taaccctgag cacgcagttc
     1681 atccgtcagg cgtggggcac cgtaacgctg ttttgaccgg gtaaaagccg cgaggacaac
     1741 gctgtcgcag tgttggcgga actgctgacg cgtgcttatc cttgtccgcc gctgacacca
     1801 cgtataccag ccgctgcggg ccacccggag cacgcggcac attgctttga tgctgaactc
     1861 agcctgatgt ttttcaataa agacatactt catttcaggc gcttcgcgaa gtatgtcgcg
     1921 gccttttgga ggatagccag ctcttcatcc cgttctgcca gctggcgttt gagacgtgca
     1981 atctcggtag acatctccag ttcacgttca gaagacgtct gctgattttg ctgtttactg
     2041 cgccagttgt agagctgtga ttcatacagg ctgagttcac gggctgcggc agtaacaccg
     2101 atgcgttcag caagcttcag ggcttcactg cgaaattcag gcgaatgctg tttacggggt
     2161 tttttactgg ttgatactgt ttttgtcatg tgagtcacct ctgactgaga gtttactcac
     2221 ttagccgcgt gtccactatt gctgggtaag atcaaatttc ctttttccaa gcggacccct
     2281 agatacctca gattcgtccg tacttggcta aaattttctc tagcgtggtt cgttgttttt
     2341 gagtcagcca tgtaaatgag ccgttgagat catacttact ctgcatgtca ctcaggaatt
     2401 tagcttcaaa tccagtaagt tgtattttcg cagttaaagc atcatgcaat gttgttttta
     2461 gcccctcatg cagacgattt tcaggcgtta aacggattga cttatcttct ttttttgata
     2521 ttggatcttt tgctagctca gtcacaaggt caatttgttt atccagttca acttgaaaga
     2581 tcagcgtatc agcaggacga ccgcgcttat caatagatag ttttatattg ctatatgtat
     2641 ttaaatcttt tgagattggt tttaaaaccc aatggtttag taatttatat tccgggtagc
     2701 ttttttctaa cattagcatg aatttaaaat catcaatact tattgaaata ttggctttat
     2761 gtgtttttct ttgtgttagt tcttttagca gccattcata aatgcgcatt gagtatttgt
     2821 tttcaaaaga cttaacgtgc tcgaggttgt atttaatgaa tctctttagg tgaaatagca
     2881 atgggattat gtatttacta aatactaaag ttacttcttg tgtttcccta ttgaactctg
     2941 catattcagc ccaattaaaa acacgcatag atacagcacc tgttggatat attaactcaa
     3001 tagaccgctt aaataattct cgtgttgatg tatttaatcg tccccacgca ttctcataac
     3061 ttatccccat aaatgaagca tattctgagc atgaaaattt aaccgttcgt tcagtttcat
     3121 ctggtgtttc taatgtgggg tttagctttg caacacaaaa caggattagt ttcgtttcgt
     3181 gttctgttag gtcataacga ctaaccgcaa gctcatttgc tttgaatact accaattcag
     3241 ccatgactaa aaccattggt tgtgatttta aaataacata acccttggta ttggttatgt
     3301 caattacact ggcataacct tttgtgtggt tgtgtggatt acctaatttt cacttaacct
     3361 tttggcggaa gatgctaatt tttgcttaac ctttcctaat ttttgcttaa cctttcatgt
     3421 ttttttttgt tttataacaa cagcttaaaa tctatagaat aaagaaagaa tagaaaagat
     3481 aaaaaagaat agatcatggc cttgtggata actgcacgcc tgagtcagtt gatcgatatg
     3541 ataaaaggat gtcgcaaacg ctgtttgctc ctcttcaaaa cagaccttaa acccccaaaa
     3601 tactgagata gcaccctcgc aagctcgggc aactcgctga acatttcttt tgtctccgac
     3661 catccggctc ctgaatcgct gttcattctg tgaaaatcag ttcgcttcgc tcacagctct
     3721 ggcagggaat ggggctagat gtcactacag ccgcttttca tgggttcata cgaggaaatc
     3781 cactcatgaa acaagaaaag cccgctacgg gcttctcagg gcgttttatg gcgggtctgc
     3841 tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct
     3901 gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca
     3961 gcggtatcat caacaggctt acccgtctta ctgtcaacca gacccgccag gataagcaat
     4021 ccggcagact ggtacagagc atggtcacgg gctttacggg cggctctggc ttcggctcgc
     4081 ttttctgcct gtatcaggtt catgactttc gctctggctt cctgcgcttt ctctgctgcc
     4141 tgttcagcct tcatcagcag ggacagcgtt ttgatatcct gtgctgtttt atctgcttta
     4201 tccgtcagta tcagcataag tttctgttgc tcattcggcg actttaagcc gcgaatgtac
     4261 tgcactctgt ctgctgccca ttgttcctgc ttttcgcttt tattcatgcc cgtttatcct
     4321 ctgaatgtta acgggataat tatgctcaat ggtggcagct aaggtcaaat tccactacaa
     4381 tccctgataa ctttttatta tcagggcgca cttagacgtt tcttcacttc gttcagaaac
     4441 ggtgcgcgcc ttcagcggga taaaaacacg atggcctcct atcacctcag cgtaaaaacc
     4501 ggcgggaaag gcagtgcctc tccccatgcc gattatattt cgcgtgaggg aaagtatgcc
     4561 cgtgaaaaag actccgacct tgagcacaag gagtccggca atatgcctgc ctgggcagcg
     4621 cataaaccga cggagttctg gaaagccgct gacacgtttg aacgtgccaa cggctgtacc
     4681 taccgtgaaa tcgagattgc cctgccgcgt gagctgaaac ccgaacaacg ccttgaactg
     4741 gtccgcgatt ttgtccggca ggaaatcggc gaccgtcatg cgtatcagtt cgccattcat
     4801 aatccgaaag cggcgatagc cggcggcgag cagccgcacg cgcatatcat gttctctgag
     4861 cggatgaatg acggtatcga ccgtgacccg cagcagtatt ttaagcgagc taacagcaaa
     4921 gcccctgagc gcggcggtgc gaaaaaggca cgtttcgggg aaacgccgac agaacgcaaa
     4981 gagcacctcg tcgcccagcg ggaacgctgg gctgacctac agaataagca ccttgaacgg
     5041 tatcagcata ccgaccgcgt tgatgcacgc tcactcaaag ctcagggcat tgagcgtgag
     5101 cctgagcggc atcttggcgc gggacaggtt cagcggttcg atactgcgca gttacaggcc
     5161 atccttgagc gcagggacgc tgaaaggcag gcggagcagt ctcgtgatga acgtgacagt
     5221 gtgattgacg tgaccacctc gctacgggaa gccatcagcg agcgggataa cctgacgata
     5281 aaacaagagc agaagcctga acctgcgcag gaagacactt ccggcagggt tttcgatttt
     5341 gagaaagagt cggagaaact gaacgcacgg gtcagtgatg ccatgaagga catacaggaa
     5401 gaaatcgacc ttcagtccct tgtgaacgat gcgatggcgg agtttcaggg gatacatcag
     5461 gaaatggagc gactggcaga aaaacagcgt cagcaggaga aagaacagca gagactcgcc
     5521 gaacagacac gacagaagcc ggataaaggc tggtcatttt cacgatagac aggatttttc
     5581 atgagcgacg aaattaaaca aatcgctgcc ctcatcgggc atcatcaggc gctggaaaag
     5641 cgtgttacct ctctgactga gcagtttcag gacgcctcag cacaattaca gcagcagagt
     5701 gacacgctga gcaggctcat tcgtgagctg gacagcgcgt ccggtaatat gactgaaacc
     5761 gtcaggcaat ctgttcatgc cgcgctcatt caggttgaga aagagctgaa acaagccgga
     5821 ttagcacagc agaaaccggc gacagaagcg cttaatcagg ctaccgatac ggcgaaggcg
     5881 atgatccatg aaatgcgacg cgagatgtcg cgctatacgt ggaaatccgc gatttatctc
     5941 gtgctgacga tatttttcgt catggctgcc tgcgtgacgg catttacgtg gtttatgaat
     6001 gacgggtaca gccagattgc tgaaatgcaa cggatggaag cggtctggca gaagaaagcc
     6061 ccgctggctg atatatcgcg ttgtgacgga aagccctgcg ttaaggtgga cacccgagct
     6121 acgtatggtg ataaagagaa tacctggatg attatcaaaa aataacataa cggcgcacat
     6181 tgcgccgtta ttgttgtcgt gaattacagc ggcagggaga gcgaagcggt ttccagcgat
     6241 tcgccctcat tcagccggtt ttgcatgtcg ctgacgaagg cgtccatctg actgttggca
     6301 aagaatttaa ttccgaccag cctgacgtcc tcattttcat caatcagggg tccgcttgga
     6361 aaaaggaaat tatcctacgc taagcgcatt ttttgcacag atggctaccg actgaacgta
     6421 acgtgtgagt catgatcaga aatgtgttaa ctctaggaat tgacacgact tactgttcga
     6481 tggggaaatc atgtttagct atctcaaggc tatgtatcat caatcaaaaa ttcaggctga
     6541 actcaaggca cagatacatg aacaaactac ggttaatgct atctgtcatc accctgaatc
     6601 aatcgagatc attgcggttt gttcgactga tgcgtattac cgaaaacgta aggacgctgc
     6661 ttttctgaca acctgttctg tgctcatgcg tactttaaaa gatgaatccg taccgatggt
     6721 actacggaaa acagcatggc gattattgaa cgaaagatac caacgaatta agttaaatca
     6781 ggcttatagg atagaaaatt tcttgctttt tgtcgatttt gaatatgctc tcgaagaaca
     6841 tgatgaacta gcagagtagt actagtgagg cgatgaatac cgacagttaa cgtgcatgtg
     6901 acttaaacgt ccgaaaccga acgattacgt acagcatgta gacaacatgt ccgaaagtaa
     6961 gcaaaaccat taacggacag ccggatcatt tcgtacatcc cttacggtca ggggttcgcg
     7021 ctcaatggaa cgaaatcacc cgttagctta aaacgcttaa cgtaggataa tttccttttt
     7081 ccaagcggac ccgcactact gcgacagaaa tgcttatagc aattgttgcg cctcgctgta
     7141 ccaataacat ggcttctgca aaatcttttt cccacgttct actttgtgcg agtttttgca
     7201 aagttttttt tagttcaaca atgcgaccca tttttatttc ctttttacgg ttcttacatt
     7261 gtatataata tcagtgagcc gcctcgttgt ggagtttttt tattctgttt tttgcgtgtc
     7321 atcgtcattg aaaaactaca gcgcccccgt ggggcttgct caactctgaa taatatcaaa
     7381 agcgagtttt gatattattc agagttctgg taacaaatat taatattgaa agttaaaatg
     7441 ttatcagcaa atcacttctt tgaaagttcg ctatacgccg tgccgtgcgt gtccattatg
     7501 tcatcaaact caaaccgtcg ctcaagcgcc ttaaacagcg cctcagcctg ttcgtgaagg
     7561 cgctcgcact cagttgcttc tgtatcgaga tcgagcacgt cgagcttttc cagtagcagc
     7621 agcgactggc ttcgaatgaa ctttgccatg ttcagggctg cggattcctg ttttgtcatc
     7681 ttttcaccta cttcatgatt tttttgttcc gaaattttct accagtcatt tttgcgcata
     7741 attattcaag aggggaacaa attttggctt tttttgtggg gaggtgtggg cgttccgttg
     7801 cgcgggtatg aaatttgtta ttttacaatt ggttatattg atttttttgt gtttattaat
     7861 gtgtaatatt acacacataa attattgtat ttcgtggttt tttttgaaaa atcttgtgtt
     7921 ttcgctcgca ggagcgtaaa acgaaaaaac ccgccgtagg cgggttatcg ggaagttgaa
     7981 gcagcaaact tcattttccg gtgggaaacg gtctaggaaa ccagttcccc gtagacgtgc
     8041 tttactcaga agcgacgctt gatttgattt taaggtcaac tgcgaatctt cgcaagccct
     8101 ctacgagaaa ctcctgacca ttttccccaa aaaaataggc tattgcttgt taccgcgtcc
     8161 gtgcgggtgt tctgagattg gatcaagagt ttttgttgct cagaattcgg atagtcggac
     8221 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctacctaacc ggaattgagt
     8281 gtcaggcgtg gtatgagaat acgcggcctt taacagcgga tagacaccga tagccgtatg
     8341 gtaggaacac aggagcgcac gagggagccg cagcgatgga aacgcgctgt atcttgatag
     8401 ctgtgtcggg tttcgccgcc tactgatttg agcaccggaa aacgtatttt tctgtgaagc
     8461 tcgtcagggg ggcggagcct atggaaaaac ggcaacgccg tggcctttcc tcttttttct
     8521 ccacaccctg ctagccctgt tatcccctgt ttctgttgat aactaagcat catgcctttt
     8581 gagtgcgctg gcaacgctcg ccgcagccga atgaccgaac gtagtgagcg aatgagcgag
     8641 gaagcggaat agcaccagga cgaacaaaaa gcacttacgc gccgaggctg attttggtgt
     8701 cacgtcacgt taaatacaat tgcgattttt cgtgttgcgt cacgggtgta ccacagaaag
     8761 ggcgtgatca gattggtgga acaacctgat cacgtatcga caaacaaaac atcatgaggc
     8821 tttaaaatgt caacaatccc aatctacagg ctgatgagtt gacagtcaaa gccagtttaa
     8881 atttacggca tgtcataaaa aagccccttt tttgacttaa cttgtcttgt gttatacaat
     8941 gtataacaca agacaagggg gatagtatga acaaaaacat gacgaaagcg atccggttga
     9001 atgacgaaga ttttaacttg tttgagtctt acgccaacgc aaaaggaata accttcagcg
     9061 aattgtgcaa aagtgcagta cgagaaaaaa ttgaggacga gttagatttg caatgtgcta
     9121 atgaaagcta cgcggattat ctcagcgaca aaacgacaat cagccacgac gaattgttta
     9181 aatctatgtg aggtagtgag gtatgacacg ctaccggaca gaatacacaa aaaggtttat
     9241 taaagaattt aagaaaatcg acacggtaca ccagagggtt atccagaagt acattgaggc
     9301 gaatattaac aacgcgctca atccgcgcct gacgggtaaa attttgaaag gagataaagc
     9361 cgaatttatc cgctggcgta taggtgatta ccgtctgatt gccactattg aagacgataa
     9421 attgctggtc attgcgttaa gcgtgggaca cagaaaagac gtttacaaaa agttttaagt
     9481 aaaccgaaat gcgtgacagg cggcgcatat gccgcctttt ttatgcccgg agggcatggg
     9541 aggcggagcc ggaagccccg cagcgaagcg aggaaaaaaa tcgtagcgcc gtatggcgtg
     9601 acaatccacc accctgccag accccttaat tgccgaccag acattccggc aatctttctt
     9661 ttttctttaa tcttaaaacc aacaataaag tttatttttt tattttccat aatgaataat
     9721 aaacctaagg aaggtgcgaa taagtcacca aacataaaaa tgcgcctttg taagtatttg
     9781 aatttaataa actaattttt ttcagtttcg ttgtggaggg tgcttattcg caccctccct
     9841 aactaatata catttgtaat tttaaactaa ttaaaatttt tactcccctc ccttctggcc
     9901 tgggtatgat tatattaaaa caggccagaa agagagagag aagggattac cactctatat
     9961 taataccagc attatatgtt acaccttcaa agttacctgt attagtggca atccccgact
    10021 taaatgcagt atcttcatta aatctatatc cggaaccaac agcaatagct gtatctgaat
    10081 tatatccacc aactgctgca gttaagttaa atttaccaac accatatggt tgaaataaac
    10141 cattaagtgc cgcctgtgat
//
