LOCUS       NZ_CP056864             2694 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00107 plasmid pRHBSTW-00107_4, complete
            sequence.
ACCESSION   NZ_CP056864
VERSION     NZ_CP056864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148489
            Assembly: GCF_013794885.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00107
  ORGANISM  Citrobacter sp. RHBSTW-00107
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00107
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.8044097125314x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 13:53:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,362
            Genes (coding)                    :: 5,204
            CDSs (with protein)               :: 5,204
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 31 of 158
            Pseudo Genes (incomplete)         :: 121 of 158
            Pseudo Genes (internal stop)      :: 25 of 158
            Pseudo Genes (multiple problems)  :: 16 of 158
            Pseudo Genes (short protein)      :: 1 of 158
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Citrobacter sp. RHBSTW-00107"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00107"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2742635"
                     /plasmid="pRHBSTW-00107_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(1864..2694,1..186)
                     /gene="mobV"
                     /locus_tag="HV072_RS27205"
                     /old_locus_tag="HV072_27210"
     CDS             join(1864..2694,1..186)
                     /gene="mobV"
                     /locus_tag="HV072_RS27205"
                     /old_locus_tag="HV072_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_167593153.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEAT
                     PRQQAEFFAHSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYQSINRGTRSQVSISPDALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGLAGTIAMASQSAQNAKRVRELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            complement(209..535)
                     /locus_tag="HV072_RS27210"
                     /old_locus_tag="HV072_27215"
     CDS             complement(209..535)
                     /locus_tag="HV072_RS27210"
                     /old_locus_tag="HV072_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_228288709.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQMDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1056..1661)
                     /locus_tag="HV072_RS27215"
                     /old_locus_tag="HV072_27220"
     CDS             complement(1056..1661)
                     /locus_tag="HV072_RS27215"
                     /old_locus_tag="HV072_27220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359292.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEEPGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     Typing          1865..2694
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagcagaaac gcctacagag cgttacagag ccttttaagg gcctttccag agagcagatg
       61 accgaaatcc tgatgatggc tcagcggttt aagcagcaaa accaggaaaa ggaaaaacag
      121 cagcgtattg aacgtgaaaa acagcgacag acgcgatcgc gtggcatggg tggtatgagt
      181 cgttagtttt gatgcgacat tccagagatc agcttctacc gccctgtata acgctgaggc
      241 cgttttttag tgcttttttg tggttgctga cagtaccttt ccaaccgaac tggggattca
      301 agcgataagt tttgctccgt cctatcttag gaccttccag tatgatcccg aaatcaatca
      361 ggttttttac cgcacgactg acatttgttt tttgcatagt cagcgcttcc gcaatatcca
      421 tctgcgcaac ttggatgtag ttctcgtaat ccagttctgc gagcaaggcc atcagcacac
      481 gcatctggtc atggtttagc tcattggcaa tggtaattag cgcggcttgg ttcatagtaa
      541 agtgcctctg gaatgatgac ttttgtttag gacgaatcac tgctacaaaa ccacctaatt
      601 cctcccctgt agtttggtct acctgcgtga ttctccgcat ctaaaaatct ccattcagga
      661 aaaaatagtt atctctcaag ataactaagt tatctctaga gataactatt gtcaaaaact
      721 ttccgcttgt ggcgcggctt cccagccatc cccttctgta tcttattact ctccacaatc
      781 acacatcggc aaaatcaggt gcttttatgc ctgttctgag tgttagggtt cctatgtaag
      841 tgacgtggaa attccgcttc ctcgctcatt cgctcactac gttcggtcat gagtctgcgg
      901 cgagcggttc ttgatttttt aaaacgatat tcggagcttt atttcgtcaa gggcgagcgt
      961 agcgagttca tttacccttg acgatgcact gtaaaaaaca cttccggaag tgtggttggt
     1021 gtcacaaagt gatgccaacc acctgtagac tgggtttaat cttttttaag cagtttatag
     1081 ccatctctaa tgctaaatag cgtgtgcata aacacaacgt ttctccccag tattctctgg
     1141 atctcagacg cggttagtgg tttttcgtac ctaacaaagc ctgatagctt ctggtagtag
     1201 gtcttgtctg gcacgccatt atgatctttc tttaaaacat caccactggc gataccatgt
     1261 gcaacaattc cttcgccgga gcgataaaga aagactaaat ctcctttttt aacctgctcc
     1321 attttttcct tataaccatc ttcaaatgtt gccgctacgc cctctgaaat catgaactta
     1381 tcatcttctt catgatgaac tctgttagtg ttaaccacat aacaattttg gtataagcct
     1441 tctgaaaact cagcgccagg ctcctcagcc atttccttta agctttcatc atcaaagatg
     1501 tattcctttg ctaggaggta gaatagctcg ttccttttat aaccagattg ctgggttgct
     1561 aatgtcagta aatggtcctc ttcttgggtt aacctaacgt taatcgtcac ttctatttgg
     1621 tctgtttgat tttttttgtt tagcgatttt atgaatgaca tcttactttc ctcaccagag
     1681 ttaatgtact aatcaatgat agtgaatcac tacattgaaa cagaagcatt gatcacatct
     1741 ttgatttaag caatgacttt atgtcatata ttttttatta agttgaattt aacgaacgat
     1801 tttgcgtagc aaagtctagt gagtacacta tcatcctgat agtggcccgc cggaggcatc
     1861 gcaatgcgat cccccgactg gctggatgta cgccagaacg agggggtaag catggcgaat
     1921 tacgcgataa tgcgctgcaa aaagctgacg ggaatgggca gtgtggccag cgctttgcag
     1981 cactgctaca gggagcgtga gacgccgaat gctgacgctg aacggacacc agaaaactac
     2041 tgctctgtgt ctcagtcggc agatgaggcg atgggaaagc tgcgggagct gttgccggaa
     2101 aaacgccgta aagatgcggt actggctgtt gagtacgtta tgacggcaag ccctgagtgg
     2161 tggaaagaag cgacaccgcg gcagcaggcg gagtttttcg cccattctga gcagtggctg
     2221 gagaagaaat acgggaagga tcgtgttgtg gctgcggtag ttcaccggga cgaggccacc
     2281 ccgcatttgt cagcgttcgt ggtgccattg acgcaggatg gacggctgtc cgcgaaagag
     2341 tttattggcg ggcggtcaaa gatgcgtgac gatcagagca cctacgctga atcagtaaaa
     2401 aaattgggtc ttgagcgcgg catcgaggga agccgtgcca cgcatcagac agttcagcac
     2461 tactaccaat cgataaaccg tggcacccgt agccaggtat caatctcgcc agatgcgctg
     2521 gagccgcgcg tattgcggaa aggaattttc accaaagacg tggaagatca ggcagccata
     2581 gccaaacgtc taagtcacgc agtgaacgat ggccttgcag gaaccatcgc catggcctct
     2641 cagagcgcgc agaatgcaaa aagagtgcgc gagctacaga agactatgga ctcg
//
