LOCUS       NZ_CP056860             1916 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00113 plasmid pRHBSTW-00113_7,
            complete sequence.
ACCESSION   NZ_CP056860
VERSION     NZ_CP056860.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148492
            Assembly: GCF_013792545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00113
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.8595558871389x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013792545.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:50:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,487
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,185
            CDSs (with protein)               :: 5,185
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 75 of 178
            Pseudo Genes (incomplete)         :: 104 of 178
            Pseudo Genes (internal stop)      :: 29 of 178
            Pseudo Genes (multiple problems)  :: 24 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056860.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00113"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00113_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(144..446)
                     /locus_tag="HV075_RS27255"
                     /old_locus_tag="HV075_27265"
     CDS             complement(144..446)
                     /locus_tag="HV075_RS27255"
                     /old_locus_tag="HV075_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            1565..1801
                     /locus_tag="HV075_RS27260"
                     /old_locus_tag="HV075_27270"
     CDS             1565..1801
                     /locus_tag="HV075_RS27260"
                     /old_locus_tag="HV075_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1227..1400
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(757..1248)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 tgaggaaagg tgctggaaac ggccctgatg cagcaggatt gcgctgaggg cgcctgcggg
       61 ggtgcgggca agggatgtta tgtttaatcg gtctgaccgt caggtacaaa aaaagccctt
      121 agggggcttt tttttgctat gagttagcca tattttcgtg cgaatttttc atcggaatcg
      181 catagataaa taataaactc aataaacgcg atgatcgcgg gtatgaatgt ccagcaaaaa
      241 attagataca ggaacccctg tcctattttt cctaagtaaa atttatgtgc gccaaatcca
      301 ccaaggaaga atgctaatag cgcggctgcg attctacttt tttcacctga acctgaagag
      361 gcgtttgtgg ctccgcagtg cgggcatgac tttgctgttt cgtgtatttc cttgccacat
      421 cctcggcagt aaaccattga actcattttt tatcctcgag cttattttaa ttgggatcat
      481 aatatccgat gttttataac gatgtgtatc ctatgtgctc ggattggata gatttccatt
      541 acggtgaagg atcttcttga gatcctttct gtggcgtcgt aatctcttgc tctgtaaacg
      601 aaaaaaccgc cctgcaaggc ggtttttcga aggttctcag agcagcaact ctttgaaccg
      661 tggtaactgg cttacgagga gcgcagtaac caaatctgtt ctttcagttt agccttaaca
      721 ggcgcataac ttcaagacta actcctctaa atcagttacc agtggctgct gccagtggcg
      781 ataagtcgtg tctttccggg ttggactcaa gatgatagtt accggataag gcgcagcagt
      841 cggactgaac ggggggttcg tgcatacagt ccatccagga gcgaactgcc ttcgcggaac
      901 tgagtgtcag gcgtggaatg agataaacgc ggccataaca gcggaatgac accggtaaac
      961 cgaaaggcag gaatgcgggg gagcacgagg gagccaccag ggggaaacgc ctggtatctt
     1021 taagccgcat cgggtttcgc caccactgat ttgagcgtca gatttcatga tgtttgtcag
     1081 gggggcggag cctatggaaa aacggcttcg ccgcggcctt atctcttccc tgttaagctc
     1141 cttcctggta tctcccagga aatttccgcc ccgcctgtaa gccggcaccg ctcgccgcag
     1201 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaatatatcc tgtactacat
     1261 atcctgctga cgcgctggtg ccgccttttt tctcctgcta catgaagcac ttcactgatt
     1321 tccacatccg tgccaacata gtcagccagt atacactccg ctagcgctac gtgactggtt
     1381 cagggctgcg ccccgaaacc cgctaaaagc taatgacgcg cctgcggctt gtccaacgcc
     1441 tcacggcacc ggattaaaat ccgctcccgt tcaccgtttc tgactgcctt tcaccgtcgc
     1501 tggcgcttca tcgtttctac tgtgtctggc agactgtgct gatttattct gactggagaa
     1561 cgttatggcc agactgacaa acttgacgcc tgcagagaaa aaatttcttg atgatgctgt
     1621 tgccgcagct gaaagagcgt caggtaaaaa gctaaatcag cctaaccggc acatcgtcct
     1681 gaacagagcg cgtgcgcaaa ttgagttgca gcgttacgcc gaccgccagc gtgcattacg
     1741 tgaggatgag cggcagcagt ccgattttgc ctggtcacgt ccgcgcgcgc cgcgccgctg
     1801 attttggctt ttggcggttt agtggtgctg acgctgcgaa atcacctgcc attcacctgc
     1861 gtgcatagcc atgcagtcgc gcagaaatca tcaggggcgt ttttccggga tactgg
//
