LOCUS       NZ_CP056859             2330 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00113 plasmid pRHBSTW-00113_6,
            complete sequence.
ACCESSION   NZ_CP056859
VERSION     NZ_CP056859.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148492
            Assembly: GCF_013792545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00113
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.8595558871389x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013792545.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:50:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,487
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,185
            CDSs (with protein)               :: 5,185
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 75 of 178
            Pseudo Genes (incomplete)         :: 104 of 178
            Pseudo Genes (internal stop)      :: 29 of 178
            Pseudo Genes (multiple problems)  :: 24 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00113"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00113_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2192..2330,1..1034))
                     /locus_tag="HV075_RS27250"
                     /old_locus_tag="HV075_27260"
     CDS             complement(join(2192..2330,1..1034))
                     /locus_tag="HV075_RS27250"
                     /old_locus_tag="HV075_27260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_021549431.1"
                     /translation="MDLTIEQRRIANAKIMIAKELATNSGTFIEIQIDRMIKEIVRKVK
                     ISASDASKEQYRKLYKSCTSKSDGDLKSCLMSCKTRASFDKTRTSFRFCIAEEIQRLRR
                     ESEKAMKAKDYDLGKKKIQEAFKLAVKFEDEFLSENRIIFNDVKSQKDFKRVSFSKKKG
                     LKKAPSADEIIKSFGFNNNINDRYALAFSVIATFGIRPAELQKGVKLQFHDGMIYAVVQ
                     GAKVGSDKGQKVRAMGSALRENNEGDKILMDAIYKAGGEVVVTQNKKDYESLRKYLNRH
                     HPGTSLYTYRHKVASDLKASGLQKRQIAEFLGHRTTDSQQYYGYSRSGSGGRSMTAKGS
                     NDVRVKPDIKDYLKPATKPAAVPLGSKLKQAKTSISTTSKAPKFKPPGIK"
                     /name="site-specific integrase"
ORIGIN
        1 actctcacat cattagaacc cttagccgtc atagaacggc caccgctgcc ggatcttgaa
       61 taaccgtaat actgctggga gtccgtagtt ctatgcccca gaaactcggc tatctggcgc
      121 ttctgtagac ctgacgcctt caggtcactg gcgactttat ggcggtatgt ataaagcgat
      181 gttcccggat gatgtctatt taaatacttt cttaaactct catagtcttt tttattttgc
      241 gtaactacaa cttcaccacc tgccttataa atggcatcca ttagaatttt atcgccctca
      301 ttattttcac gtaatgcaga ccccatagct cttacttttt ggcctttatc cgatccaact
      361 ttagcacctt gtacaactgc ataaatcatt ccatcatgga actggagttt aacccctttt
      421 tggagttctg ccggacgtat tccaaaagta gctatcacag agaacgccag agcatagcga
      481 tcattgatat tattgttaaa accaaatgac tttattattt catctgcaga aggtgctttt
      541 tttaatcctt ttttcttact gaatgaaacc cttttaaaat ctttttgact ctttacatca
      601 ttaaaaataa ttctattttc agataagaat tcatcttcaa atttaaccgc tagtttaaat
      661 gcttcttgaa tttttttctt tccaagatcg taatctttag ccttcatcgc tttttcagat
      721 tcacgcctta acctttgtat ttcttctgca atacagaacc taaaggacgt tctcgtttta
      781 tcaaagcttg ctcgcgtttt acaactcatc aagcagcttt tcagatctcc atcgctcttg
      841 gatgtgcatg atttgtataa ctttctgtac tgttccttgg atgcatcgct agccgagatc
      901 ttaacttttc ttactatttc ttttatcatt ctgtcaattt gaatttcgat aaaagtaccg
      961 ctattggttg ccaactcttt tgcaatcatt atttttgcgt tggcgatcct tctttgttct
     1021 attgttaaat ccatattacc ccctgcttta tattttattc ctccatcgtc tttatatgcc
     1081 catctcccaa tgcgaaaggt cagaaagctg gcgcaatctt cccaatcgca aaggtcgaag
     1141 ggcaaggcgt ttttgctacc gcaaacttcc attttcaaaa gcgaaaatgt aagacacgcc
     1201 tacattattg ggacataaaa acactgtccc aataatgtta aaagcataag tcccaataat
     1261 gttatatata tattttaact tttattttta ttctgtctat ctatccataa acagagagag
     1321 tgcggagcaa aaagtggcat cgccaaaaaa aatgtttttc cggtttttag tccggtgcgt
     1381 aagtgctcta tattcattta gtggatatta cgcttcctcg acaaaataaa atattttgtc
     1441 ttgcggcgag cgttgccgtt tttctttatc tcttttggtt gtacttactc tgtataagcc
     1501 ttcggcctga gctacgcaaa ggaacaccaa agaggccgtg aagccaccct ttcgggtgtt
     1561 tcacccaaag tttcgcctcc tcgaattcat cacgaattca agttgacgat cctttggtaa
     1621 agcttctttt ttgagcttca cccaaagagc agcgacttag agcttttcac cgtttccaat
     1681 gaactggcac cctgttgcac ccccgctgtc gccacatagc actacttgca gaatcctaac
     1741 cccatttatg gctgggcttt ctcatagctg gcctgaatcc ttaccaagcg ccttagtgac
     1801 tcgttcccgg tctggtcttt tgcaacactc cccgtttaga cccacataca ctgtttggcg
     1861 aacaatcaca tgccagtcac ttggaaaggc ggctccacac caaacaccgg acacaatcgg
     1921 tttaacaaac tatgtatgct taaggatatg aaaatgcagg tgattttatg aaacttgtaa
     1981 agaagtggga tttgactttt acggaaatgt aagttaactt ttgccttaca tcacgtaatc
     2041 atgtgctcga atcacttgat tacaagactc aaaaagtcgc caaacttttt cagtccctca
     2101 aaaccagcgc aagctggttt tgttgtatct ggatcctgtt ataaccgatt tgcgttatac
     2161 gtcaatagtc tatcaaaaat attgaagcat cttatttgat acccggaggc ttaaacttcg
     2221 gcgctttcga cgtggtggaa atcgaggttt tagcttgctt tagcttgcta cctaaaggca
     2281 ctgcagcagg ctttgtggct ggttttaagt agtcttttat atctggttta
//
