LOCUS       NZ_CP056826             2265 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00137 plasmid pRHBSTW-00137_5, complete
            sequence.
ACCESSION   NZ_CP056826
VERSION     NZ_CP056826.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148508
            Assembly: GCF_013790835.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00137
  ORGANISM  Citrobacter sp. RHBSTW-00137
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00137
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.8124067554789x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 13:57:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,379
            CDSs (total)                      :: 5,263
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 45 of 173
            Pseudo Genes (incomplete)         :: 132 of 173
            Pseudo Genes (internal stop)      :: 36 of 173
            Pseudo Genes (multiple problems)  :: 33 of 173
            Pseudo Genes (short protein)      :: 1 of 173
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056826.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Citrobacter sp. RHBSTW-00137"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00137"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742638"
                     /plasmid="pRHBSTW-00137_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(502..849)
                     /locus_tag="HV091_RS26705"
                     /old_locus_tag="HV091_26710"
     CDS             complement(502..849)
                     /locus_tag="HV091_RS26705"
                     /old_locus_tag="HV091_26710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110958373.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(1538..1684)
                     /locus_tag="HV091_RS27140"
     CDS             complement(1538..1684)
                     /locus_tag="HV091_RS27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_114456554.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKAPLAAATGKRVYKRLLM
                     QSS"
                     /name="replication initiation protein"
     Typing          complement(1516..2065)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.374"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ccggcccgct aaagactcct ggttagctcc cgtatgtttg ctatcttttt gctaccattt
       61 tgctactttt ttgctatcaa aatgctacct ctcccttctt gcaataaatg accaaggcac
      121 gttagcgatg tatacatcgc cgcgccgcaa gggtgcccct tccgaatccc gttcatcact
      181 gcgtgctgat tcatccacgt taaatccgac ccccttagca ataaatcctt ccatgcctga
      241 cggcctggcc aacacctcac ggcagcacag taaactgcgc acccgttcac cgtttcaggt
      301 aaggcgaaag atactctttt ttggggttgg gtttgaattc aggctgaaac ctgatgccag
      361 gcagccggct gcataacgat tcacagcctc agaaatcatc agcgcgtttc tgagcggttt
      421 tcagggggta agatgtgttc ttttgcaggt gaggtcgtag aaacgccctg agagcctctc
      481 aggcggtgcg ggcaacggat gttatggtaa atgttggata gcaaggacta tcagtccggc
      541 aatgctacat atcattgcta atggatttac gagcaaaatg ccgaatgttg tcgcagataa
      601 tccgcgaaca atgatattgg caagaagtcc gccaacaatg agggataaaa aggtataacc
      661 agcaactttc agtaacgaca gatctcccca tatcacctct actatcggat agataaaaac
      721 accaacgcca gcaggaagcg tagtaaccat caacagcaaa tggcttttag taggaccgta
      781 ctctctgaca tttccaactt tcatcatgtg tgtgatgaat gacgatatcc accataggat
      841 catgatcata aggaaattca taattactta ctctcgatgc gtagatcagg tgctttctgt
      901 agatttggtc gtacatactc gctgtctctt tcgtcaagac aacggaagta gagtgttact
      961 gtggtatcta tccccattct tgtgttagcc acgttttcgt tagtgagcag gaatttttta
     1021 ccgaggctat tacatttctc agaacctgtt ttaaaggcac tttgacgtgc tgatgccgca
     1081 tctctaaaac catcagcagt tgctgaaacc gtatatgtat ccctacctaa ctcaagaacg
     1141 ttgctggtag aaacacaacc aactgtaagc agcgatattg ataaaatacc taacctataa
     1201 atcattgatt ttattaccca agttgggtgg ttgtggatca tccaactata cgatttttat
     1261 gctttatttt caaagggttt tgtcggtgtg agtttttgac tggtactgtt gtttggtatc
     1321 ggttttactt tgatgtctgt cggtcatcga agatgaacct cttgagatcc tttccgttgc
     1381 gtcgtaatct cttgctctga aaacgaaaaa accacctggg gaggtggttt ttcgaaggtt
     1441 cagtaagttg gggaacttct gaaccgtggt aacagggtgt acaagaccgc tgccaccaaa
     1501 ttcgtccttc cagtttagcc gtagtttggc tctgacttca agaactctgc atcagtaatc
     1561 tcttgtacac cctcttacca gtggctgctg ccagtggcgc tttgtcgtgt ctttccgggt
     1621 tggactcaag tcgatagtta ccggaacagg cgcagcagtc ggactgaacg gggggttcgt
     1681 gcatacagtc cagcttggag cgaactgcct tcccggaacc gagtgtcagg cgtggaatga
     1741 gataaacgcg gccataacag cggagtgaca ccggtaaacc gaaaggcagg aacaggagag
     1801 cgcacgaggg agccaccagg gggaaacgcc tggtatcttt aagtcctgtc gggtttcgcc
     1861 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     1921 acggcttcgc cgtggccttc ttgcttccct gttaagcttc ttcctggaag atgccaggaa
     1981 attcccgccc cgccagtaag ccggtaccgc tcgccgcagc cgaacgaccg agcggagcga
     2041 gtcagtgagc gaggaagcgg tatatctccg ggcttatctt tggcaccgta cgcgccatag
     2101 ctgcattttt taatacgatg caggataggg ctaacgccgc aaagtgacgt cacctgacgt
     2161 tcctggatta caaaggttaa agcagcggac aggaaatgtt ttgtgcccac agctatgctg
     2221 gcttattcac aaaacagcgg gacaagatgt gtttttggag taccg
//
