LOCUS       NZ_CP056764             4479 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00186 plasmid pRHBSTW-00186_5,
            complete sequence.
ACCESSION   NZ_CP056764
VERSION     NZ_CP056764.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148531
            Assembly: GCF_013744615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00186
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.8987752265686x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:32:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,791
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,556
            CDSs (with protein)               :: 5,556
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 53 of 119
            Pseudo Genes (incomplete)         :: 88 of 119
            Pseudo Genes (internal stop)      :: 30 of 119
            Pseudo Genes (multiple problems)  :: 42 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056764.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00186"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00186_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3580..4479,1..228)
                     /locus_tag="HV114_RS28800"
                     /old_locus_tag="HV114_28815"
     CDS             join(3580..4479,1..228)
                     /locus_tag="HV114_RS28800"
                     /old_locus_tag="HV114_28815"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_181333272.1"
                     /translation="MPHINTSNKLTCIEGFCGAGGMSLGLKQAGFDVRLAFDINENAVE
                     TYNKNIGNHCEKLDASIVSGKFLLEKAGIKTRLDLFSGGPPCQGFSKQRRGAHLLDDDR
                     NKLVLEYSRLVNELKPRAFLFENVEIFGQKRGKDLIDEIKIKLFDYNIYTYFICSSDFG
                     VAQKRGRFLMIGIDKSEGKIVPKLLKSDKTLTIRDVIGQLPPPPEDYKEHPDIPNHIKC
                     KITKINEERFSHVPPGGGWKDIPYDLRLKCHQKVDTASGGWPDVYGRLEWDKQCPTITA
                     GFDSFTRGRYGHPSQNRAITLREGAMLQGFPVNYRFYGNRDAIRLQIGNAVPPPVSYAA
                     GTAIINCLDTKQDKNHFNFQNPMELDKDGQFSLAI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(212..1117)
                     /locus_tag="HV114_RS28805"
                     /old_locus_tag="HV114_28820"
     CDS             complement(212..1117)
                     /locus_tag="HV114_RS28805"
                     /old_locus_tag="HV114_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032490083.1"
                     /translation="MDILKEKIDVASRLYNLNLDHIPATLQVIEHAMLLLKNNAGYGYF
                     GSFNGKNTQEYHSFTFNGEYSRPVRDDLFITDYDFFVSGFREFNESLRDIGSKWSSFDS
                     RRANKIIYTSVMSVACCFDLWKSGSRKTPGTFFEIFMAAVLKWMIPDEIFSKHIPLIDQ
                     LESDDESIDPSSVSTDIVIKSKYKNASVVIPLKITTRERIVQPFAQQRILDSYFGNGVY
                     FSFLACISETQQDKKKKKVNHICVPGTIRLYQKYLSSLSGMYYCDIPERYLERDLTDII
                     PVRTMGDFLFDIYSFFRSQG"
                     /name="hypothetical protein"
     gene            complement(1927..2328)
                     /locus_tag="HV114_RS28810"
                     /old_locus_tag="HV114_28825"
     CDS             complement(1927..2328)
                     /locus_tag="HV114_RS28810"
                     /old_locus_tag="HV114_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087834902.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMIDTSE
                     LLRVFGPFEQPRGEHHSGQVSEQRDTAFADQSEQVIHHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2328..2519)
                     /locus_tag="HV114_RS28815"
                     /old_locus_tag="HV114_28830"
     CDS             complement(2328..2519)
                     /locus_tag="HV114_RS28815"
                     /old_locus_tag="HV114_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_155006913.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDFDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2935..3048
                     /locus_tag="HV114_RS28820"
                     /old_locus_tag="HV114_28835"
     CDS             2935..3048
                     /locus_tag="HV114_RS28820"
                     /old_locus_tag="HV114_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_155006912.1"
                     /translation="MHEPPVQSDYHACSGNYQLESNPERHDKAPLAVAIGN"
                     /name="replication initiation protein"
     gene            complement(3223..3423)
                     /locus_tag="HV114_RS28825"
                     /old_locus_tag="HV114_28840"
     CDS             complement(3223..3423)
                     /locus_tag="HV114_RS28825"
                     /old_locus_tag="HV114_28840"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_235606871.1"
                     /translation="MAGIATPTIADFERGARKPHGNNLRSIIIAFENKGLDFVEEGGEI
                     IGIFIRRKNVRAEESIDLSGH"
                     /name="hypothetical protein"
     Typing          2666..3052
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.33"
                     /coverage="90.0"
                     /evalue="4.8700000000000004e-167"
ORIGIN
        1 acattaaggg agggcgcaat gctacaagga ttccctgtaa actatagatt ctatggtaac
       61 cgtgatgcta ttaggctaca aattggaaat gctgtcccac cgccagtgtc ctacgcagca
      121 ggaacagcaa taattaattg tttagatacg aagcaagata aaaatcactt taacttccaa
      181 aatccaatgg agttggataa agacggacaa tttagccttg cgatctaaag aaagagtaaa
      241 tatcaaataa gaaatcaccc atagttctga ccggtattat atcggtcaga tctctttcca
      301 agtaacgctc tggaatatcg cagtaataca taccagagag actggagaga tatttttgat
      361 ataatcttat tgtccctgga acacatatat gattaacctt tttcttcttc ttatcttgtt
      421 gcgtctcgct aatacacgct aagaaagaaa aataaacacc atttccaaag tatgagtcca
      481 atattctctg ctgggcaaat ggttgaacga tcctctctct tgtagttatc tttaaaggta
      541 taactacact agcgttttta tatttagact ttataacaat atcagtagac acgcttgatg
      601 gatcgattga ctcatcatct gattcaagtt ggtctattag cggtatgtgc ttcgagaaaa
      661 tctcatcagg aatcatccat ttcaacactg ccgccatgaa aatttcaaaa aatgttccag
      721 gagtctttct gctaccactt ttccaaagat caaagcagca tgcaacagac attacagatg
      781 tataaattat tttattagct ctcctagaat caaaggatga ccatttacta ccaatatccc
      841 tcaaactttc attaaattct ctaaaccctg aaacaaagaa atcatagtca gtgataaaga
      901 gatcatctct tactggtctt gaatattcgc cattgaacgt aaatgaatga tattcttgtg
      961 tattttttcc attaaatgaa ccaaagtaac catatcctgc attgtttttt aataacaaca
     1021 ttgcatgttc tattacctgc agtgttgcag gaatatgatc caagtttaaa ttataaagcc
     1081 gtgaagcaac gtcaattttt tctttcaaga tatccatgtt tttcccatgc cttttctgcg
     1141 ttaattttgt ttattagtga atgtttttca tataaataga gctactttca tttaccataa
     1201 catccgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga aaccggtaaa
     1261 acaggcaaca aaccacccga gaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     1321 catgagtatg cgcgcaagtg catagcgcgg aaattcacac cattcagcca ggggaagtcc
     1381 tgtttccggc gttcttctcc tccgaaaacg gtgaacgggg acgcaattta ttgcgtggtc
     1441 gtgagccgtt gccggcgcag ccggaaaacc ccttttcagc acgttcagga atcgggttca
     1501 tcgacggccg gcaatgacgt aatggcaagt cgccggacga ctcaccacac gattcaccgg
     1561 cgactcttca ctagagtcgt acctagagtc gccgcgtcta aaaaacaacc aaacggagag
     1621 tcgggtctag agtcgggtcg ttattaactc attgttttta tagtcctgag tcgttaccga
     1681 ctctatgacg actcgccggg gactctatga cgactcgtca tagagtcgga tctatataaa
     1741 taaaatttaa aaaacgaaat ttcaggaaag taaactactg atattaaata taaatctgca
     1801 aaattttaat tttgcgtggg gtgtgggtat ttttcgtggt gaggagcgtt cagagatctg
     1861 acgatttttg ggaaggttca ggaactcccc aggatggccg gagaggaaaa ctcaagcatg
     1921 gcgggatcag gattttttcc agaatttcca ccatggcgcc ggggattcag ttaattttgc
     1981 ctggtgattt tcaagaagga gcatggcctg tttcaggctg tcaatatgcg aatccttgga
     2041 caaaagtaag gtattcaggt gttcaatctc ctgtttcaga tgatgaatca cctgttcact
     2101 ctggtcagca aaggctgtat cacgctgttc agagacctgt ccagaatggt gttcacccct
     2161 cggttgctca aaggggccaa agacacgcaa aagctctgaa gtatctatca tttttgcatt
     2221 gcgttcaccg ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gtgttgttct
     2281 actcttaccc gtcagtcggg cagcctcact aatagacact ctggccatca gttaccatcc
     2341 tgtaatgcat ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt
     2401 ttctcacaaa ggtcggcctc agcatcaaaa tcaagctcgt taagcttctc cagcaggagc
     2461 agagactgat tctggatgaa tttagccata ttcagcgctg ttttctgttg tttagtcata
     2521 aagggcctca gagcgtcagt gctcaatgta aaactggtct cttttccgct tcctcgctca
     2581 ctcagtcgct atgctcctgc cgttcggctg cggcgagcgg tagtaactca cgtataaggg
     2641 cataagacga cagcacagag ggccagaagg aacaaaagaa ggccgcggcg ttgccgtttt
     2701 tccataggct ccgcccccct gacaagcatc acagaaacgg acgctcaaat cagtggtggc
     2761 gaaacccgac aggactttaa agataccagg cgtttccccc tggtggctcc ctcgtgcgct
     2821 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     2881 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     2941 gaaccccccg ttcagtccga ctaccacgcc tgttccggta actatcaact tgagtccaac
     3001 ccggaaagac acgacaaagc gccactggcg gtagccattg gtaactgatt tagaggagat
     3061 agtcttgaag ttatgccccg gttaaggcta aactgaaagg acaagttttg gtgactgtgc
     3121 tcctccaagc cagttacctc ggttcaaaga gttgctagct cagagaacct ttgaaaaaac
     3181 cgccttgcag ggcggttttt tctttccgat aagatgatgc cctcaatgac ctgacagatc
     3241 aatagattcc tcggctctga cattctttct tctgataaag atcccgatta tctcaccacc
     3301 ctcctcaaca aaatccagcc ccttgttttc aaaggcaata attatagatc tcaagttatt
     3361 gccatgaggt tttcttgcgc cacgctcaaa atcagcaatg gttggtgtcg caattccagc
     3421 cataagagct agctctttct gagtcaagtt aactaaggcc ctagaggcct tacaaatttc
     3481 aggagtcata tttttaatat gattttgtat caaaacattg acctctaggt ataattaacg
     3541 ctatcataca tgtaaacggc cccttgtaag tgagacatca tgccacatat aaacacatct
     3601 aataaactaa cgtgcatcga aggtttttgt ggtgccgggg gtatgtccct cggtctgaaa
     3661 caagcaggtt ttgatgttag gttggcattt gatattaatg aaaatgctgt agaaacctac
     3721 aacaaaaaca ttggtaacca ttgcgaaaaa ttagatgctt ctattgttag cgggaagttt
     3781 cttttggaaa aagcaggcat aaaaacgaga ctagatcttt ttagtggtgg gccgccttgt
     3841 caggggtttt caaaacaaag aagaggtgcc catcttcttg atgacgatag aaataagtta
     3901 gttcttgaat attctaggtt agttaatgaa ttaaaaccta gggctttttt atttgaaaat
     3961 gtggaaatat ttggtcaaaa aagaggcaaa gatcttattg atgaaattaa gatcaagctg
     4021 tttgattata acatatacac atatttcatc tgttcatctg attttggagt tgcgcaaaaa
     4081 agaggccgtt tcctaatgat tggaatagac aagtcagaag gtaaaatcgt ccccaaacta
     4141 ttaaaatcag ataaaaccct tacaataaga gatgtaattg gtcagttacc ccctccacca
     4201 gaggattata aagagcaccc agacatacct aatcatataa aatgtaaaat aacaaagatt
     4261 aatgaggaaa ggttttcaca cgtccctcct ggcggaggat ggaaagatat accatacgac
     4321 ttaaggttaa aatgtcacca aaaggttgat acggcatcgg gaggatggcc tgacgtttat
     4381 gggagacttg aatgggacaa gcaatgtcca accataacag caggcttcga tagtttcact
     4441 agaggcaggt atggacaccc ctcacaaaat agagcaata
//
