LOCUS       NZ_CP056763             4667 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00186 plasmid pRHBSTW-00186_4,
            complete sequence.
ACCESSION   NZ_CP056763
VERSION     NZ_CP056763.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148531
            Assembly: GCF_013744615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00186
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.8987752265686x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:32:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,791
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,556
            CDSs (with protein)               :: 5,556
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 53 of 119
            Pseudo Genes (incomplete)         :: 88 of 119
            Pseudo Genes (internal stop)      :: 30 of 119
            Pseudo Genes (multiple problems)  :: 42 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00186"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00186_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            19..1575
                     /locus_tag="HV114_RS28775"
                     /old_locus_tag="HV114_28790"
     CDS             19..1575
                     /locus_tag="HV114_RS28775"
                     /old_locus_tag="HV114_28790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_045326453.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            2635..2958
                     /locus_tag="HV114_RS28780"
                     /old_locus_tag="HV114_28795"
     CDS             2635..2958
                     /locus_tag="HV114_RS28780"
                     /old_locus_tag="HV114_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3167..>3601
                     /locus_tag="HV114_RS29135"
                     /pseudo=""
     CDS             3167..>3601
                     /locus_tag="HV114_RS29135"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3636..4121
                     /locus_tag="HV114_RS28790"
                     /old_locus_tag="HV114_28805"
     CDS             3636..4121
                     /locus_tag="HV114_RS28790"
                     /old_locus_tag="HV114_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            4125..4340
                     /locus_tag="HV114_RS28795"
                     /old_locus_tag="HV114_28810"
     CDS             4125..4340
                     /locus_tag="HV114_RS28795"
                     /old_locus_tag="HV114_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2350..2522
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(1873..2425)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2636..2952
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          2949..4444
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatatggagt gtatttttat gagaattaaa aagcctcacc ttcgtccgat agtcataacc
       61 ttgtccgttg gtgggatttt gttaacttct atttttctga ttgccgttat tatggttttt
      121 cagcgcgaaa atatagaaaa agatttgctt gatgctaatt catcgtacgc tatgaaaatg
      181 tcagatgtaa tgagtggtta tattgaaatg gctcaaggtg aattagcata tggagcaaaa
      241 aaaataaaat caactaatga tattgactca cttaaagatg aggcggatag gttaagattg
      301 cagtctggta tgtttaattc agtttttgtt gttagtaatg atgctgttgt tctggcgact
      361 tcgccagaat ctcttgattt agttggtacc cgcttgaatt caagtgccag caaattggcg
      421 attgaggaaa agaaaccatt catatcttca ccgttcaaat cagttactgg gaatttgatt
      481 attttgttgt cacatcctat ttatgataag aatggcaatt acatagggca tgtcggtggg
      541 tctatatatc taaaaaaaca tagtttattt agcgatgttt tgagcaggca ttttttcaag
      601 agcgacactg aaattagcat tgttagtgat gatggaactg tgatctttaa taacgataac
      661 agtgctgttg gtcggcccat ggttatgtct gatgatttga aatcaaagtt aacggagtct
      721 gatagaggtg agggggaatt ttttttttcg gggaatagat atcttctggg ttatgctcat
      781 atgaaaaata ctgcttggaa tgtgttggtt tatagccatg ctgacagcgc gactactatt
      841 cttattactt atgctaaaag tgtacttgtt ctgctaattg taataattat tacattttca
      901 cttctttcat atttcattgc ctcccgtatt tcacttcctt tagaaaagct tgctatatct
      961 accaatgcta aagatatagg ggcttcgtta gtttatatta aagctatcaa tgcttggtat
     1021 gccgaagctg atcgacttaa aaaggcactt tttacgcatg ttaaggtaat gatggaaagg
     1081 gttgattctt taaatgaggt ggcgcttaaa gatcccctta ccggcgtgaa caatagattg
     1141 gggttttcaa ctaaaataca gaataacaag tatggggcag gagcttcagt tattgctatt
     1201 gatgttgatt tttttaaaaa aataaatgat agtttcgggc atggggtcgg agatgaggtg
     1261 cttgtttctt tggctcagat tattaagtct tgttgccgta gtgaagatat tatttgtcgg
     1321 tttggtggag aagagtttgt tgtttttctg ccgaatacat ctgtggtaac agcggagtgt
     1381 gttgcggagc gtattaggag tgttattgaa cgtactgttt tccctaataa cttgcgtgtc
     1441 accgtatctg ctggtgtggc aacacaagat gatcctcttg gtgggatcga ttttttgctg
     1501 aaaaatgctg acgatgctct ttatcaagct aagagagagg ggcgtaataa agtgattgta
     1561 tatagcgcta tttgagtttt tttgatgacg ctttacgcgc tagtgtgccg tttttttttg
     1621 tggggagggg ggttcaagaa gcggatcctc ttgttatccc ttcgatgtcg tcgtaatctc
     1681 atgccctgca aacaaaaaaa ccaccgctac caacggtggt tcgtttgccg gaatcaagag
     1741 ctaccaactc tttcttccga aggtaactgg ctttcacgga gcgcaggtac caaaactgtc
     1801 cttccagtgt agccgcagtc aggccaccac ttcaagaact ctgtagaaca ctctactact
     1861 ccgctccgtg aatcctctta ctaatggcta ccgccactgg cgatttttcg tgtctgttcc
     1921 gggttggact caagatgata gttaccggga caacgtggta gtcgggctga acggggggtt
     1981 cgtgcataca gtccagcttg gagcgaactg ccttcccgga accgagtgtc aggcgtggaa
     2041 tgagataaac gcggccataa cagcggattg acaccggtaa accgaaaggc aggaacagga
     2101 gagcgcacga gggagccacc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     2221 aaaaacggct tcgccgtggc cttatcgctt ccctgttaag cttcttcctg gcatctccca
     2281 ggaaatttcc gccccgcctg taagccggta ccgctcgccg cagccgaacg accgagcgca
     2341 gcgagtcagt gagcgaggaa gcggaatata tcttgtatca catattctgc tgacgctccg
     2401 ctgctgcatt tttctcctgc cacatgaagc atttcactga cactcgcatc cgtgccaaca
     2461 tagtaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa
     2521 cccgccaaaa gcctgacgcg cctgcggctt gtccaacccc gcgccgaccg ggaaaggctt
     2581 tcccgcccgt cccggcgtta tcaggaggcc ggtttgtcag agaaacggaa caagatgctc
     2641 accatgtggg tgacggagga cgagcaccgg cggctgcttg agcgctgcga cggcaggcag
     2701 ctggcagcgt ggatgcggca gacctgcctg gacgaaaagc cggcacgttc cggaaagctt
     2761 ccctcaatct cgccggcgct gctgcgccag cttgccggca tgggcaataa cctgaaccag
     2821 atagcccgcc gtgttaacgc cggcggcggt accggccatg acagggtgca gattgttgcg
     2881 gcgctgatgg ccatcgatgc cggacttgag cggctgcggc acgccgttct tgagaaagga
     2941 acggacgatg atcgttaaat ttcatccacg cggtcgcggc ggtggcgccg ggccggtgga
     3001 ctatctgctg ggtaaagacc ggcagcgtga aggcgccacc gtcctgcagg ggaagccgga
     3061 agaagtccgg gagctgattg acgcgtcgcc ttacgttaag aaatacacct ccggggtact
     3121 gtcgtttgcg gaagcggatt taccctccgg ccagcgggaa aagctgatgg caagctttga
     3181 gcgggttctg atgcccggac ttgataaaga ccagtacagc atcctgtggg tggagcatcg
     3241 tgacaagggg cggcttgagc tgaattttct gatcccgaac acggagctgc tgaccggcag
     3301 acggctccag ccgtattacg atcgcgccga ccgtccacgc atcgatgcct ggcagaccat
     3361 cgtgaacggc agactggggc tgcacgaccc gaacgcaccg gagaaccggc gggcactggt
     3421 cacaccgtct gcactgccta aagcgaagca ggaggctgca gaagcgatta cacgcggtct
     3481 gcttgctctt gcctcatccg gggagctgaa aacccgtcag gatgtcactg aggcgctgga
     3541 aagcgcaggt tttgaggtgg tccgcaccac aaaaagcagt atcagcattg ccgacccgga
     3601 cggggggcga aacatccgac tgaagggagc catttatgaa cagtctttta acgctggcga
     3661 aggacttaga gcagaaatcg aaagcgcagc agcagagtac cggcgagatg ctgaaagccg
     3721 cattcagcga gcacgagaaa tctgtaaaag cggaactgaa cgaaagcgcg aagagaatca
     3781 gcgccgccat cctcgaccac gaccggaaac tgtcctcagc catgagccag cgcacgaaag
     3841 gcatggtgcg catggtcagc cagacgtggc tgaccatcgt cctggtctcc gcgctgctga
     3901 tcgcctcgag cgcgggcatt ctgtggtggc aggggcagca gatactcgac aactacacga
     3961 ccatccggga gcagaagagc acgcaggcca tgctgtcaga gaggaacagc ggcgtgcagc
     4021 tctcgacctg cggcgagcag agacgccgct gcgtgaaggt gaacccggaa gcgggacggt
     4081 tcggagagga ttcgagctgg atgatactgg cggggaaata gcacatgacg gagctggaaa
     4141 aacagttgct gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt
     4201 gggagagcgc cttcgcggaa tggcggacga tgtctggtct tatgcaacgg gagaacgcag
     4261 cgctgagcga gcgcgtcacg ggcttgagca ggcaggtgca gagtttgagc gagcagctgc
     4321 gccggttgtc gagcgactga atgccgtcga gctgcaccgg gagcaggagc gcgtggtgca
     4381 gcatcagaaa gcgctggagc tggagcgatc tcagcgacag cggacgtata acagcccttc
     4441 actgtgataa cctgataaaa cggggctttc ggtggacatc tgcgctgaaa gccggagcca
     4501 ttcacccgac tgcatagcca tgcagccggg tgaaatttat ctggcgcaga atgcgcggtt
     4561 tagcggagag tttgctgccg gttttaccgg gctgacgccg catttgttcc gaacccgtcc
     4621 ggggcggtgc tgataaggga tgttatggta aatgcggcat tttttat
//
