LOCUS       NZ_CP056759             2495 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00198 plasmid pRHBSTW-00198_4,
            complete sequence.
ACCESSION   NZ_CP056759
VERSION     NZ_CP056759.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148534
            Assembly: GCF_013744415.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00198
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.202796037712x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013744415.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:07:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,700
            CDSs (total)                      :: 4,586
            Genes (coding)                    :: 4,494
            CDSs (with protein)               :: 4,494
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 60 of 92
            Pseudo Genes (internal stop)      :: 18 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056759.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00198"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00198_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            480..707
                     /locus_tag="HV117_RS23355"
                     /old_locus_tag="HV117_23365"
     CDS             480..707
                     /locus_tag="HV117_RS23355"
                     /old_locus_tag="HV117_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1073..1258
                     /locus_tag="HV117_RS23360"
                     /old_locus_tag="HV117_23370"
     CDS             1073..1258
                     /locus_tag="HV117_RS23360"
                     /old_locus_tag="HV117_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1549..1767)
                     /locus_tag="HV117_RS23365"
                     /old_locus_tag="HV117_23375"
     CDS             complement(1549..1767)
                     /locus_tag="HV117_RS23365"
                     /old_locus_tag="HV117_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1306..1393)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2020..2494
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.188"
                     /coverage="91.0"
                     /evalue="2.76e-175"
ORIGIN
        1 atcgccattg gcaacggcca ttggcaaaca ggatgtgcga gagatgtctc aagagttctt
       61 gaagtggcgg ccttagtgag gctacactga aaggacaaat ttggtgacat gtctcgcaca
      121 agactgttac cacggttagg ggatctgcca ggattaccta accttcgaaa aaccacctgc
      181 cagggtggtt ttttcgttta cagggcaaga gattacgcgc ggaaaaaaag gatctcaaga
      241 agatccttta taaattagac atccaacctc acaaagagga atacttaaat catcttggta
      301 tatgtatcgg ccggattaca tctcatataa gcctcctccc cgcctgacac cacactttga
      361 ccatagcacc tcgcgcctaa ctcgatatac agcccaccat caagcctctt ttcacataaa
      421 aaatgaccta tagttagatg gttagtgacc aaaactgata tggataaaag gagctggctt
      481 tgaaaaagat ttttgtttca gtgttcgcag cggcagttgc gctttcagcc ctcaccggct
      541 gcacgcgcac aagctatgcc atacacacca atgacggtcg cactattgtc agcgacggca
      601 aacctaaaga atcagactca ggcctgcttg gctatacaga cgcgaatggt gtcaaacagc
      661 agatcaataa aaccgacgtt aaagaagtct ccgaaattcc gcactgagca tcttctccga
      721 aaaaggccgc taatgtggcc tttttcttta cctgtggttc tccgccaaaa tcccagcaaa
      781 ttgcatcacc aaagctaaaa gctttcaggg ttgtctaaaa agcacaagac ataagaggaa
      841 gtgcggtatt ttataatcaa gcccccaaga atttaccata acatccgttg cccgcaccgc
      901 ctgagacgcg ttcagcgcgt tcctgacgaa accatgacaa aaaccacaac aaaccacccc
      961 ggaacccgtc agaaacgcgc ctgttaaatt ttaacggcat gcatgactat gcaccagaat
     1021 gacgccatgc tcttttcacg caaaaatcat caccagacgg ggaaaatcac cagtgaccag
     1081 acaggaatcc gccgccctca atatggccaa atttatccgc gcacagacac ttctcctcct
     1141 tgagcggctc gagcagatgg atctggatga ggctgccggc tgctgtgagc acctgcacga
     1201 tcaggccgaa gcgctttacg ccatgctgaa cgcacagata ggcgaggaaa atgcgtgaaa
     1261 atcggtgaac gggtgcgcaa ttcagtgcgc ggccgtgagg cgatggcggg gtgtcggggc
     1321 gcagccctga ccagggtatt tgtgatgccg gcgcgtgcgc ggtattacaa atgcacatcc
     1381 tgtcccggaa cggacaccgg gaaacagcaa aaaaaaccgg gcggcacgcc cggaactcaa
     1441 tcaagttaga ttagattact ctcactcgtc cataacagca tcatggaacg acgaccaccg
     1501 tccgtgacgg ccgcctcgtt taagtatgga cagaaataca gaaaatgctc aggacgaaat
     1561 gtaatgaatg cgaacggatt caagaaattc gagcatgaca gtccttacgg ccggttcggt
     1621 ttcagacaaa atctgccggt atgcatccag catcatggct ccggcatccc ctccggcacg
     1681 ccgtagccag accgaaacaa cggacacaag caggtgtcgc tcatcatcac taagagtcat
     1741 cagggctccg gaagaaaaac caaacatggc ttacctactg ttaacggcaa cgcagaagaa
     1801 aggtttacag ggtagcgggc agggatgaaa aaaaaaaaat tgcagtcagg ccggaacggc
     1861 tgctgaaccg gtacaggcgg gttccgggcg cagcgccgga ccagtcacgg agcactagcg
     1921 gagtgtatac tggcttaaga tgtagcatgg cagtaacaca tagcattaat gccacataag
     1981 acaggcgcag aaagtgaaag aaaacgcgtc aggagaaaat gtgatacaga atatattccg
     2041 cttcctcgct cactgactcg ctccgctcgg tcgttcggct gcggcgagcg ggacgggctt
     2101 acggatggat cagaaatttc ctgggggatg ccaggaagat acttaacgaa gaaagcataa
     2161 ggccgcgcca aagccgtttt tccataggct ccgcccccct gacaagcatc acagaatctg
     2221 acgctcaaat cagtggtggc gaaacccgat gcggcttaaa gataccaggc gtttccccct
     2281 ggtggctccc tcgtgctccc ccgcattcct gcctttcggt ttaccggtgt cactccgctg
     2341 ttatggccgc ggtttctcat tccacgcctg acactcagtt ccgggaaggc agttcgctcc
     2401 aggatggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     2461 tatcaacttg agtccaaccc ggaaagacac gacta
//
