LOCUS       NZ_CP056758             4784 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00198 plasmid pRHBSTW-00198_3,
            complete sequence.
ACCESSION   NZ_CP056758
VERSION     NZ_CP056758.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148534
            Assembly: GCF_013744415.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00198
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.202796037712x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013744415.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:07:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,700
            CDSs (total)                      :: 4,586
            Genes (coding)                    :: 4,494
            CDSs (with protein)               :: 4,494
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 33 of 92
            Pseudo Genes (incomplete)         :: 60 of 92
            Pseudo Genes (internal stop)      :: 18 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056758.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00198"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00198_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            422..>544
                     /locus_tag="HV117_RS23640"
                     /pseudo=""
     CDS             422..>544
                     /locus_tag="HV117_RS23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(864..1220)
                     /locus_tag="HV117_RS23320"
                     /old_locus_tag="HV117_23330"
     CDS             complement(864..1220)
                     /locus_tag="HV117_RS23320"
                     /old_locus_tag="HV117_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(1423..1740)
                     /locus_tag="HV117_RS23325"
                     /old_locus_tag="HV117_23335"
     CDS             complement(1423..1740)
                     /locus_tag="HV117_RS23325"
                     /old_locus_tag="HV117_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1927..2169
                     /locus_tag="HV117_RS23330"
                     /old_locus_tag="HV117_23340"
     CDS             1927..2169
                     /locus_tag="HV117_RS23330"
                     /old_locus_tag="HV117_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(2841..3056)
                     /locus_tag="HV117_RS23335"
                     /old_locus_tag="HV117_23345"
     CDS             complement(2841..3056)
                     /locus_tag="HV117_RS23335"
                     /old_locus_tag="HV117_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3063..3554)
                     /locus_tag="HV117_RS23340"
                     /old_locus_tag="HV117_23350"
     CDS             complement(3063..3554)
                     /locus_tag="HV117_RS23340"
                     /old_locus_tag="HV117_23350"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3589..4293)
                     /locus_tag="HV117_RS23645"
                     /old_locus_tag="HV117_23355"
                     /pseudo=""
     CDS             complement(<3589..4293)
                     /locus_tag="HV117_RS23645"
                     /old_locus_tag="HV117_23355"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4232..4555)
                     /locus_tag="HV117_RS23350"
                     /old_locus_tag="HV117_23360"
     CDS             complement(4232..4555)
                     /locus_tag="HV117_RS23350"
                     /old_locus_tag="HV117_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4671..4783)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.491"
                     /coverage="99.0"
                     /evalue="9.68e-46"
     Typing          27..535
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(2750..4242)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4239..4555)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
ORIGIN
        1 ggcggcaacg gcgcgtcagc agaatatgcg atacaggata tattccgctt cctcgctcac
       61 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg cgggcttacg aacggggcgg
      121 aaatttcctg gaagatgcca ggaagaaact taacagggaa gcgataaggc cacggcagag
      181 ccggttttcc ataggctccg cccccctgac gagcatcaca gaatctgacg ctcaaatcag
      241 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      301 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
      361 ttgtctcatt ccacgcctga cactcggttc cgggtaggca gttcgctcca agctggactg
      421 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctgt tccggtaact atcgacttga
      481 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccactggta acaggattag
      541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      601 cactggaagg acagtatttg gtatctgcgc tctgctgaag ccagttacca ggttaagcag
      661 ttccccaact gacttaacct tcgaaaaacc gcctccccag gcggtttttt cgtttacaga
      721 gcaagagatt acgcgtacag agcacggatc tcaagaagat cgtaagaaga aagttaacat
      781 gagcgttttt atccctcaaa aagaaggctt ctagttatag aagccttctt tttgagggat
      841 aacttatatt tttttaaaaa aaatcatgca atagcatgtt tcttctcaga actatcaaca
      901 atgtgtttcc tttctatgat ttcatgagcg ggaatcactc tgtctatttc ttcttgcaat
      961 cgagggctac tagtaacctc ccataaatca acagactgct gtatatttag ccagaacttt
     1021 gcagtggtat cgaaggcttt agccaaccta aaagccatat cggtagacag ctttctatta
     1081 ttgttaacta aagcactggc agagtttcta tgcacattca acatttcagc caagtcattg
     1141 atttttaaac cacttggttc gagatattca taaagaataa tatcccctac agttgatggt
     1201 tttctagttg cttgggacat gctttactcc taaatttttt acttctctct gttagatgag
     1261 gaagataagt atttcgtcta ttaccagctg tagcgatgag taattatctt aaatatagtc
     1321 acgtcatttt ttttattaat cattatttgt aatgacattt taatgtaggt gagggaacgc
     1381 ctcacctact tgttttataa gataacagcc acgacctgtt atctatcggt aatcatgtgg
     1441 atcgagatac aaatcctcga ctccgttttc accccactta aatatcaagc ggtacttctc
     1501 attcacccgt atggatgaat accctgtcaa tggagggttg agattctcaa atcggtttcc
     1561 cgggggagac cgcaaatctc caactttatc agcagcattc agcatatcca gcttgcgacc
     1621 taatgaagca ctaatcgcag ttggaatctt tcgattggct ttaccatatt tgtaaaactg
     1681 gtatagccaa tcgtctcgaa actgaatgcg gcaatcatca tgttcatgat cttgctgcat
     1741 ttttctttta cctcaatttt tgccgcaact tggcaatttt ttcgatcacc ttcttacctc
     1801 cgatatagta cagcaaacat atcaaatgca caacagcaca gtgcacttgt gcaatcaatg
     1861 gctatgctgt agaatgcact catgcacagt gcgggtgtgc aaaataaatg ataatttgga
     1921 ggttgcatgg ctcttgttcg ctcaggaaac aaaagcaaga tttttgaagc agatacgaag
     1981 tttaactcgg tttttgacac tgaatataag ccgggagaaa catgtgaata tgaagggatt
     2041 tataaatgta ccggttgcgg taaagaagta actatcgcag gcgggaaaac aatcccacca
     2101 caaaaccacc accaacatac ccccgcgcaa gggaacattc gttggcaatt gtttgtttat
     2161 gctcagtgag gtatagaaaa tggcagatcg tcgaccactc aacccacacg ttgtcgttcc
     2221 taaaccaggc ggcggaactc aacctcgtga ccgtaacaat gacggaaggg tcagaggaaa
     2281 acgctcagat gcaggaaagc cacgaaaata acagcaagcc gccagcctgg cggctttttt
     2341 atatctgcag ctgatactga tttaccataa catccgttgg gcgcaccgct gaaacggggt
     2401 tggctgcgtt tccggtgcca gacgcgagaa agtggcgaca gacataacga aaaaacacca
     2461 gaaacgcagc cgctgaatat tacactggtg catagtcatg cagccagatg catggatgtt
     2521 aaagtcacac taaaatccgc gccaatgcct taaaaaccga caaatgcttc tccagccagt
     2581 cccacagctc cgccgccaga tacaggcagt taagcaatga ttcaatgaga tttaacagct
     2641 tcatttcccg ctcctgaata agtcgtgacc tttttgatca cgaacctatt tagcgggaga
     2701 aataaagagc ccctcttcgt gcgggttgtc agctgcctgg atggtttaaa gtgtcggccc
     2761 gcggtaggtt ttctgcggct ggcgctccag ctccagtgtt ttctgatact cattctccct
     2821 ctgccggagc tcgatggcat tcagttcccg gacaagcggc gcagctgctc gcttaaactc
     2881 agcacctgcg tgctcaagtc cgacacgcgc tcgttcagca ccgcgttctc ccgttgcata
     2941 agcccacaca tgctccgcca ttccgcgaag gcgctctccc attcgtccag cctttgcgag
     3001 tagtcctgct gtagctgctc taatgcgctc agcaactgct tttccagctc tgtcattttc
     3061 cgctactcct gtttcaggcg gatccatgtc gtcccgtcgt aggggatggc ttcggtttgc
     3121 atcccgggcg gcaggataag aaacctccgg ccgtcgctgc tctcctgata gcgcactccc
     3181 cacgtccggg cagtcatttt cgccagggta tcttcctgct gacgaagatg cgtgtaattg
     3241 tcggttatct gctgcccctg ccaccacagt atgctcccgc tcgtgccgat cagcagcgct
     3301 gacaccatca ggatggtgaa ccatgtccgc ccggcagtcc gcagtacgct ccgccggttt
     3361 ttctccatcg cctcgctcat actctgctcg tgtgcgagga tggcgtcgct gattctcctc
     3421 gcgcttgcgc tcagttccgc tctgacagac tgctcgtgct cgctgaatgc ggctttcagc
     3481 atctcgccgg tactctgctg ctgcgctttc gattgctgct ctaagtcctt cgccagcgtt
     3541 aaaagactgc tcatagatgg ctcccttcag tcggatgttt cgccccccgt ccgggtcggc
     3601 aatgctgata ctgcttttcg tggtgcgcac cacctcaaaa cctgaccgtt ccagcgcctc
     3661 agtgacgtcc tgacggtttt tcagctcccc ggacgaagca agggtcagca gtccgcgcgt
     3721 gatggcttca gtggcctcct gcttcgtttt cggcagtccg gacggcgtga ccagcgcccg
     3781 gcggttttcc ggcgcgttcg ggtcatgcag ccccagtctg ccgttcacga tggtctgcca
     3841 ggcatcgatg cgcggccggt cggcgcggtc gtagtacggc tggagccgcc tgccggtcag
     3901 cagttcggta ttggggatca gaaagttcag ctccagacgc cccttgtccg cgtgctccac
     3961 ccacagaatg ctgtactgat ctttatcgag tccgggcatc agaacccgct cgaagctggc
     4021 catcagtttt tcccgctggc cgggaggcag atcggcctcc gcaaacgaca gtacgccgga
     4081 ggtgtatttc ttcacgtaag gcgacgcgtc aatcagctcc cggacttctt ccggcttccc
     4141 ctgcaggacg ctggcgcctt cacgctgccg gtctttaccc agcagatagt ccaccggccc
     4201 ggcgccaccg ccgcgaccgc gtggatgaaa tttaacgatc atcgtccgtt cctttctcaa
     4261 gaacggcgtg ccgcagccgc tcaagcccgg catcgatggc catcagcgcc gccacaatct
     4321 gtaccctgtc atggccggtg ccgccgccgg cgttaacacg gcgggctatc tggttcaggt
     4381 tattgcccat accggcaagc tggcgcagca gcgccggcga gagtgagggg agctttccgg
     4441 tgcgcgcagg cttttcgtcc aggcaggtct gccgcatcca cgccgccagc ggcctgccgt
     4501 cgcagcgctc aagcagccgc cggtgctcgc cctccgtcac ccacatggtg agcatcttgt
     4561 tccgtttctc tgacaaaccg gcctcctgat aacgccggga cgggcgggaa agcctttccc
     4621 ggtcggcgcg gggttggaca agccgcaggc gcgtcagtgg cttttggcgg gtttcggggc
     4681 gcagccctga accagtcacg tagcgctagc ggagtgtata ctggcttaat atgttggcac
     4741 ggatacggaa atcagtgaag tgcttcatgt ggcaggagaa aaaa
//
