LOCUS       NZ_CP056755             4783 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00216 plasmid pRHBSTW-00216_5,
            complete sequence.
ACCESSION   NZ_CP056755
VERSION     NZ_CP056755.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148545
            Assembly: GCF_013743935.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00216
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.964872087639x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013743935.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:41:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,718
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,510
            CDSs (with protein)               :: 4,510
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 27 of 94
            Pseudo Genes (incomplete)         :: 62 of 94
            Pseudo Genes (internal stop)      :: 24 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056755.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00216"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00216_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4681..4783,1..>8)
                     /locus_tag="HV128_RS23790"
                     /pseudo=""
     CDS             join(4681..4783,1..>8)
                     /locus_tag="HV128_RS23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(340..696)
                     /locus_tag="HV128_RS23415"
                     /old_locus_tag="HV128_23430"
     CDS             complement(340..696)
                     /locus_tag="HV128_RS23415"
                     /old_locus_tag="HV128_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(899..1216)
                     /locus_tag="HV128_RS23420"
                     /old_locus_tag="HV128_23435"
     CDS             complement(899..1216)
                     /locus_tag="HV128_RS23420"
                     /old_locus_tag="HV128_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1403..1645
                     /locus_tag="HV128_RS23425"
                     /old_locus_tag="HV128_23440"
     CDS             1403..1645
                     /locus_tag="HV128_RS23425"
                     /old_locus_tag="HV128_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(2316..2531)
                     /locus_tag="HV128_RS23430"
                     /old_locus_tag="HV128_23445"
     CDS             complement(2316..2531)
                     /locus_tag="HV128_RS23430"
                     /old_locus_tag="HV128_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2538..3029)
                     /locus_tag="HV128_RS23435"
                     /old_locus_tag="HV128_23450"
     CDS             complement(2538..3029)
                     /locus_tag="HV128_RS23435"
                     /old_locus_tag="HV128_23450"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3064..3768)
                     /locus_tag="HV128_RS23795"
                     /old_locus_tag="HV128_23455"
                     /pseudo=""
     CDS             complement(<3064..3768)
                     /locus_tag="HV128_RS23795"
                     /old_locus_tag="HV128_23455"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3707..4030)
                     /locus_tag="HV128_RS23445"
                     /old_locus_tag="HV128_23460"
     CDS             complement(3707..4030)
                     /locus_tag="HV128_RS23445"
                     /old_locus_tag="HV128_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4146..4319)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          4243..4783
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.463"
                     /coverage="89.0"
                     /evalue="0.0"
     Typing          complement(2225..3717)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3714..4030)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
ORIGIN
        1 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
       61 ggcctaacta cggctacact ggaaggacag tatttggtat ctgcgctctg ctgaagccag
      121 ttaccaggtt aagcagttcc ccaactgact taaccttcga aaaaccgcct ccccaggcgg
      181 ttttttcgtt tacagagcaa gagattacgc gtacagagca cggatctcaa gaagatcgta
      241 agaagaaagt taacatgagc gtttttatcc ctcaaaaaga aggcttctag ttatagaagc
      301 cttctttttg agggataact tatatttttt taaaaaaaat catgcaatag catgtttctt
      361 ctcagaacta tcaacaatgt gtttcctttc tatgatttca tgagcgggaa tcactctgtc
      421 tatttcttct tgcaatcgag ggctactagt aacctcccat aaatcaacag actgctgtat
      481 atttagccag aactttgcag tggtatcgaa ggctttagcc aacctaaaag ccatatcggt
      541 agacagcttt ctattattgt taactaaagc actggcagag tttctatgca cattcaacat
      601 ttcagccaag tcattgattt ttaaaccact tggttcgaga tattcataaa gaataatatc
      661 ccctacagtt gatggttttc tagttgcttg ggacatgctt tactcctaaa ttttttactt
      721 ctctctgtta gatgaggaag ataagtattt cgtctattac cagctgtagc gatgagtaat
      781 tatcttaaat atagtcacgt catttttttt attaatcatt atttgtaatg acattttaat
      841 gtaggtgagg gaacgcctca cctacttgtt ttataagata acagccacga cctgttatct
      901 atcggtaatc atgtggatcg agatacaaat cctcgactcc gttttcaccc cacttaaata
      961 tcaagcggta cttctcattc acccgtatgg atgaataccc tgtcaatgga gggttgagat
     1021 tctcaaatcg gtttcccggg ggagaccgca aatctccaac tttatcagca gcattcagca
     1081 tatccagctt gcgacctaat gaagcactaa tcgcagttgg aatctttcga ttggctttac
     1141 catatttgta aaactggtat agccaatcgt ctcgaaactg aatgcggcaa tcatcatgtt
     1201 catgatcttg ctgcattttt cttttacctc aatttttgcc gcaacttggc aattttttcg
     1261 atcaccttct tacctccgat atagtacagc aaacatatca aatgcacaac agcacagtgc
     1321 acttgtgcaa tcaatggcta tgctgtagaa tgcactcatg cacagtgcgg gtgtgcaaaa
     1381 taaatgataa tttggaggtt gcatggctct tgttcgctca ggaaacaaaa gcaagatttt
     1441 tgaagcagat acgaagttta actcggtttt tgacactgaa tataagccgg gagaaacatg
     1501 tgaatatgaa gggatttata aatgtaccgg ttgcggtaaa gaagtaacta tcgcaggcgg
     1561 gaaaacaatc ccaccacaaa accaccacca acataccccc gcgcaaggga acattcgttg
     1621 gcaattgttt gtttatgctc agtgaggtat agaaaatggc agatcgtcga ccactcaacc
     1681 cacacgttgt cgttcctaaa ccaggcggcg gaactcaacc tcgtgaccgt aacaatgacg
     1741 gaagggtcag aggaaaacgc tcagatgcag gaaagccacg aaaataacag caagccgcca
     1801 gcctggcggc ttttttatat ctgcagctga tactgattta ccataacatc cgttgggcgc
     1861 accgctgaaa cggggttggc tgcgtttccg gtgccagacg cgagaaagtg gcgacagaca
     1921 taacgaaaaa acaccagaaa cgcagccgct gaatattaca ctggtgcata gtcatgcagc
     1981 cagatgcatg gatgttaaag tcacactaaa atccgcgcca atgccttaaa aaccgacaaa
     2041 tgcttctcca gccagtccca cagctccgcc gccagataca ggcagttaag caatgattca
     2101 atgagattta acagcttcat ttcccgctcc tgaataagtc gtgacctttt tgatcacgaa
     2161 cctatttagc gggagaaata agagcccctc ttcgtgcggg ttgtcagctg cctggatggt
     2221 ttaaagtgtc ggcccgcggt aggttttctg cggctggcgc tccagctcca gtgttttctg
     2281 atactcattc tccctctgcc ggagctcgat ggcattcagt tcccggacaa gcggcgcagc
     2341 tgctcgctta aactcagcac ctgcgtgctc aagtccgaca cgcgctcgtt cagcaccgcg
     2401 ttctcccgtt gcataagccc acacatgctc cgccattccg cgaaggcgct ctcccattcg
     2461 tccagccttt gcgagtagtc ctgctgtagc tgctctaatg cgctcagcaa ctgcttttcc
     2521 agctctgtca ttttccgcta ctcctgtttc aggcggatcc atgtcgtccc gtcgtagggg
     2581 atggcttcgg tttgcatccc gggcggcagg ataagaaacc tccggccgtc gctgctctcc
     2641 tgatagcgca ctccccacgt ccgggcagtc attttcgcca gggtatcttc ctgctgacga
     2701 agatgcgtgt aattgtcggt tatctgctgc ccctgccacc acagtatgct cccgctcgtg
     2761 ccgatcagca gcgctgacac catcaggatg gtgaaccatg tccgcccggc agtccgcagt
     2821 acgctccgcc ggtttttctc catcgcctcg ctcatactct gctcgtgtgc gaggatggcg
     2881 tcgctgattc tcctcgcgct tgcgctcagt tccgctctga cagactgctc gtgctcgctg
     2941 aatgcggctt tcagcatctc gccggtactc tgctgctgcg ctttcgattg ctgctctaag
     3001 tccttcgcca gcgttaaaag actgctcata gatggctccc ttcagtcgga tgtttcgccc
     3061 cccgtccggg tcggcaatgc tgatactgct tttcgtggtg cgcaccacct caaaacctga
     3121 ccgttccagc gcctcagtga cgtcctgacg gtttttcagc tccccggacg aagcaagggt
     3181 cagcagtccg cgcgtgatgg cttcagtggc ctcctgcttc gttttcggca gtccggacgg
     3241 cgtgaccagc gcccggcggt tttccggcgc gttcgggtca tgcagcccca gtctgccgtt
     3301 cacgatggtc tgccaggcat cgatgcgcgg ccggtcggcg cggtcgtagt acggctggag
     3361 ccgcctgccg gtcagcagtt cggtattggg gatcagaaag ttcagctcca gacgcccctt
     3421 gtccgcgtgc tccacccaca gaatgctgta ctgatcttta tcgagtccgg gcatcagaac
     3481 ccgctcgaag ctggccatca gtttttcccg ctggccggga ggcagatcgg cctccgcaaa
     3541 cgacagtacg ccggaggtgt atttcttcac gtaaggcgac gcgtcaatca gctcccggac
     3601 ttcttccggc ttcccctgca ggacgctggc gccttcacgc tgccggtctt tacccagcag
     3661 atagtccacc ggcccggcgc caccgccgcg accgcgtgga tgaaatttaa cgatcatcgt
     3721 ccgttccttt ctcaagaacg gcgtgccgca gccgctcaag cccggcatcg atggccatca
     3781 gcgccgccac aatctgtacc ctgtcatggc cggtgccgcc gccggcgtta acacggcggg
     3841 ctatctggtt caggttattg cccataccgg caagctggcg cagcagcgcc ggcgagagtg
     3901 aggggagctt tccggtgcgc gcaggctttt cgtccaggca ggtctgccgc atccacgccg
     3961 ccagcggcct gccgtcgcag cgctcaagca gccgccggtg ctcgccctcc gtcacccaca
     4021 tggtgagcat cttgttccgt ttctctgaca aaccggcctc ctgataacgc cgggacgggc
     4081 gggaaagcct ttcccggtcg gcgcggggtt ggacaagccg caggcgcgtc agtggctttt
     4141 ggcgggtttc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc
     4201 ttaatatgtt ggcacggata cggaaatcag tgaagtgctt catgtggcag gagaaaaaag
     4261 gcggcaacgg cgcgtcagca gaatatgcga tacaggatat attccgcttc ctcgctcact
     4321 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtgc gggcttacga acggggcgga
     4381 aatttcctgg aagatgccag gaagaaactt aacagggaag cgataaggcc acggcagagc
     4441 cggttttcca taggctccgc ccccctgacg agcatcacag aatctgacgc tcaaatcagt
     4501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctcg
     4561 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     4621 tgtctcattc cacgcctgac actcggttcc gggtaggcag ttcgctccaa gctggactgt
     4681 atgcacgaac cccccgttca gtccgaccgc tgcgcctgtt ccggtaacta tcgacttgag
     4741 tccaacccgg aaagacacga caaaacgcca ctggcagcag cca
//
