LOCUS       NZ_CP056754             4853 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00216 plasmid pRHBSTW-00216_4,
            complete sequence.
ACCESSION   NZ_CP056754
VERSION     NZ_CP056754.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148545
            Assembly: GCF_013743935.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00216
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.964872087639x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013743935.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:41:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,718
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,510
            CDSs (with protein)               :: 4,510
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 27 of 94
            Pseudo Genes (incomplete)         :: 62 of 94
            Pseudo Genes (internal stop)      :: 24 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056754.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00216"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00216_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(226..654)
                     /locus_tag="HV128_RS23380"
                     /old_locus_tag="HV128_23395"
     CDS             complement(226..654)
                     /locus_tag="HV128_RS23380"
                     /old_locus_tag="HV128_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            939..1367
                     /locus_tag="HV128_RS23385"
                     /old_locus_tag="HV128_23400"
     CDS             939..1367
                     /locus_tag="HV128_RS23385"
                     /old_locus_tag="HV128_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1424..1915)
                     /locus_tag="HV128_RS23390"
                     /old_locus_tag="HV128_23405"
     CDS             complement(1424..1915)
                     /locus_tag="HV128_RS23390"
                     /old_locus_tag="HV128_23405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(2379..2594)
                     /locus_tag="HV128_RS23395"
                     /old_locus_tag="HV128_23410"
     CDS             complement(2379..2594)
                     /locus_tag="HV128_RS23395"
                     /old_locus_tag="HV128_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_063842553.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSNLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2601..3092)
                     /locus_tag="HV128_RS23400"
                     /old_locus_tag="HV128_23415"
     CDS             complement(2601..3092)
                     /locus_tag="HV128_RS23400"
                     /old_locus_tag="HV128_23415"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3127..3831)
                     /locus_tag="HV128_RS23785"
                     /old_locus_tag="HV128_23420"
                     /pseudo=""
     CDS             complement(<3127..3831)
                     /locus_tag="HV128_RS23785"
                     /old_locus_tag="HV128_23420"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3770..4093)
                     /locus_tag="HV128_RS23410"
                     /old_locus_tag="HV128_23425"
     CDS             complement(3770..4093)
                     /locus_tag="HV128_RS23410"
                     /old_locus_tag="HV128_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181549196.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4209..4382)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          4306..4852
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.455"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2282..3780)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.099"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3777..4093)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="1.8800000000000002e-59"
ORIGIN
        1 aggtttagag gaggagagtc ttgaagtttc gggtgcgata aggctacact gaaaggacag
       61 ttttggtgac tgtgctcctc caagccagtt accgcggttc aaaagagcgt caactcttgc
      121 gaaccttcga aaaagccacc ttgccgggtg gttttttcgt tttcaccgca agagattacg
      181 acgagggtca acgaatctca agggatcatt catccgaaca agagatcatt cgaaattttg
      241 ggggagttgc atagggtcat gatacccaag ctctaacagt tttatatggt tcggcgctag
      301 acgttggaag tggatgaccc aatcagagac tttgtacttc ccgtgaaatg tatcgatgaa
      361 aacgggatcg cctatatggg catgccacaa tttattagcc ctggcatgtt cttcaatagc
      421 ttctttgttt tcgtaactgt caggcacgtt ccaagatggc gataccttgc caatccaagc
      481 cggatggcct tttaaggcat cgccaaaaag attgtacttc tcgaaatgtt cgatgaagtc
      541 atcgatttta tcgagtgttt tttgagaaaa gcgggatgtg taataaagat tgaatgtctc
      601 agagaaagag acaacattca attgtggatt ttgcgtgctt gatccgctgc ccatgctctt
      661 ctctgctccc tacttagacc agatggcatt gtaacagatc tctcattcgc tctggaaatc
      721 aacagagaca aggatacacc aaatatttca gtatctttta caagatcatg atgttgtttg
      781 gttttcatat cactaactcc gtatttaatg cagatagaaa tgtttttctg ttctcagatt
      841 agccttttat agttgtgtaa taatggcgct ggtgtcaccc tattcatatg aatacttata
      901 ttatatatat ctcaaaaaaa atatttacag gataatcaat gagttacgca atagaaaaaa
      961 aatggaaggc tggaggtctg tcattaggat ggactgcagt acctactgtg ttgtttttta
     1021 tacaaaatga gaagaaattg agccctgtag cattcaatac cttgctgaat ctcatagtac
     1081 actggtggaa tctacaggag tggccgcacc cctcaatgga gagcctggct atacggatgg
     1141 gggtgtcagt aaggacggtg caaagggcta ttaatgacct agaaaaagcc aatctcttag
     1201 ataaaaaacc gacatcaaaa agcgacaacc gatatggtgg ccgcaacata tacgacctta
     1261 caaagcttgt agattattta gacataattg gtccttctgt gtccgagcaa gtaagaaagc
     1321 cgcgacataa aaagccaata tatacagtaa ggaaaaaagc cgattagtta tgctggttac
     1381 atgcaggaac agcccgcatt tggaggccgg gcttatcgtt aaattagggc ttaatcgtaa
     1441 gaccagaacg ttgaatgtat tcatcaacgc gatcgccaat gtttacaatt ttggcgaaat
     1501 tcacatcagc cgatgacacg tttagatccg ttgcggatgc aggcataggc aaggttgcac
     1561 gatgaccatc gacggaaacc aaggtttttc tatcaacaaa cgaagaacca tagtgaacgg
     1621 tgtactcgac tgaacacgca ttactatcag gatgacgaac agcccaaggc tcattaaacg
     1681 agttgtagtc gtcgtaatca gctctcttaa tgtgcagtgc taaatcattt ttataagtaa
     1741 acgcccctgt ctcatcatcc acaatccaat cattttgact cgattgcgca agaattcgtg
     1801 taacttcatc agcattcata aatagctcca tcaggcagtg tgtagataaa aagcaaacaa
     1861 catcacctac atgggtatta tcagaacctt tatcaacgga cggttgattt accataacat
     1921 ctgttgcccg cacagctgaa acggggttgg ctgcgtttcc ggtgccagac gcgagaaagt
     1981 ggcgacagac atgacgaaaa aacaccggaa acgcagccgc tggatattac acttatgcat
     2041 agtcatgcag ccagatgcat gccgcgtaaa gtcacactaa aatcagcgcg aacgccttaa
     2101 aaaccggcaa atgcctctcc ggcaactcac acagctccat caccaggtaa aaacagttca
     2161 gcagggcttc aataacgtta agaattttca tcacttcatc cgggataagt cactaccatt
     2221 ccggtagtca gcctatttag caggaacgcg atgagccctt cttcgtgcgg gctgtcacct
     2281 catcttcgga ccatcaaaac tcttctgctt ctgacggagc gtcagctcca gctcgttctg
     2341 gcgctgatac tccagtgcag ccttctcctc gatggcattc agttcccgga caaacggcgc
     2401 agctgctcgc ttaaactcag cacctgcgtg ctcaagttcg acacgcgctc gttcagcacc
     2461 gcgttctccc gttgcataag cccacacatg ctccgccatt ccgcgaaggc gctctcccat
     2521 tcgtccagcc tttgcgagta gtcctgctgt agctgctcta atgcgctcag caactgcttt
     2581 tccagctctg tcattttccg ctactcctgt ttcaggcgga tccatgtcgt cccgtcgtag
     2641 gggatggctt cggtttgcat cccgggcggc aggataagaa acctccggcc gtcgctgctc
     2701 tcctgatagc gcactcccca cgtccgggca gtcattttcg ccagggtatc ttcctgctga
     2761 cgaagatgcg tgtaattgtc ggttatctgc tgcccctgcc accacagtat gctcccgctc
     2821 gtgccgatca gcagcgctga caccatcagg atggtgaacc atgtccgccc ggcagtccgc
     2881 agtacgctcc gccggttttt ctccatcgcc tcgctcatac tctgctcgtg tgcgaggatg
     2941 gcgtcgctga ttctcctcgc gcttgcgctc agttccgctc tgacagactg ctcgtgctcg
     3001 ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct gcgctttcga ttgctgctct
     3061 aagtccttcg ccagcgttaa aagactgctc atagatggct cccttcagtc ggatgtttcg
     3121 ccccccgtcc gggtcggcaa tgctgatact gcttttcgtg gtgcgcacca cctcaaaacc
     3181 tgaccgttcc agcgcctcag tgacgtcctg acggtttttc agctccccgg acgaagcaag
     3241 ggtcagcagt ccgcgcgtga tggcttcagt ggcctcctgc ttcgttttcg gcagtccgga
     3301 cggcgtgacc agcgcccggc ggttttccgg cgcgttcggg tcatgcagcc ccagtctgcc
     3361 gttcacgatg gtctgccagg catcgatgcg cggccggtcg gcgcggtcgt agtacggctg
     3421 gagccgcctg ccggtcagca gctcggtatt ggggatcaga aagttcagct ccagacgccc
     3481 cttgtccgcg tgctccaccc acagaatgct gtactgatct ttatcaagtc cgggcatcag
     3541 aacccgctcg aagctggcca tcagtttttc ccgctggccg ggaggcagat cggcctccgc
     3601 aaacgacagg acgccggaag tgtatttctt cacgtaaggc gacgcgtcaa tcagctcccg
     3661 gacttcttcc ggcttcccct gcaggacggt ggcaccatca cgctgccggt ctttacccag
     3721 caggtagtcc accggcccgg cgccaccgcc gcgaccgcgt ggatgaaatt taacgatcat
     3781 cgtccgttcc tttctcaaga acggcgtgcc gcagccgctc aagtccggca tcgatggcca
     3841 tcagcgccgc cacaatctgc accctgtcat ggccagtgcc gccgccggcg ttaacgcggc
     3901 gggctatctg gttcaggtta ttgcccatac cggcaagctg gcgcagcagc gccggcgaga
     3961 tagaggggag ctttccggtg cgcgcaggct tttcgtccag gcaggtctgc cgcatccacg
     4021 ccgccagcgg cctgccgtcg cagcgctcaa gcagccgccg gtgctcgccc tccgtcaccc
     4081 acatggtgag catcttgttc cgtttctctg acaaaccggc ctcctgataa cgccgggacg
     4141 ggcgggaaag cctttcccgg tcggcgcggg gttggacaag ccgcaggcgc gtcagtggct
     4201 tttggcgggt ttcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact
     4261 ggcttattat attggcacgg gtgcgggaat cagtgaagtg cttcatgtgg caggagaaaa
     4321 aaggcggcac cggcgcgtaa gcagaatatg cgatacagga tatattccgc ttcctcgctc
     4381 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tgcgggctta cgaatggggc
     4441 ggaaatttcc tggtagatgc caggaagaaa cttaacaggg aaacgataag gccgcggcga
     4501 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     4561 agtggtggcg aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct
     4621 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     4681 tttgtctcat tccacgcctg acactcagtt ccgggaaggc agttcgctcc aagctggact
     4741 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcaacttg
     4801 agtccaatcc ggaaaaacac gacaaagcgc cactggcagc agccactggt aac
//
