LOCUS       NZ_CP056753             5321 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00216 plasmid pRHBSTW-00216_3,
            complete sequence.
ACCESSION   NZ_CP056753
VERSION     NZ_CP056753.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148545
            Assembly: GCF_013743935.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00216
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.964872087639x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013743935.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:41:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,718
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,510
            CDSs (with protein)               :: 4,510
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 27 of 94
            Pseudo Genes (incomplete)         :: 62 of 94
            Pseudo Genes (internal stop)      :: 24 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056753.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00216"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00216_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5209..5321,1..115)
                     /locus_tag="HV128_RS23345"
                     /old_locus_tag="HV128_23360"
     CDS             join(5209..5321,1..115)
                     /locus_tag="HV128_RS23345"
                     /old_locus_tag="HV128_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(645..854)
                     /locus_tag="HV128_RS23350"
                     /old_locus_tag="HV128_23365"
     CDS             complement(645..854)
                     /locus_tag="HV128_RS23350"
                     /old_locus_tag="HV128_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(858..1343)
                     /locus_tag="HV128_RS23355"
                     /old_locus_tag="HV128_23370"
     CDS             complement(858..1343)
                     /locus_tag="HV128_RS23355"
                     /old_locus_tag="HV128_23370"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1378..2082)
                     /locus_tag="HV128_RS23775"
                     /old_locus_tag="HV128_23375"
                     /pseudo=""
     CDS             complement(<1378..2082)
                     /locus_tag="HV128_RS23775"
                     /old_locus_tag="HV128_23375"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2021..2344)
                     /locus_tag="HV128_RS23365"
                     /old_locus_tag="HV128_23380"
     CDS             complement(2021..2344)
                     /locus_tag="HV128_RS23365"
                     /old_locus_tag="HV128_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2983..3141
                     /locus_tag="HV128_RS23780"
     CDS             2983..3141
                     /locus_tag="HV128_RS23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            3528..4648
                     /locus_tag="HV128_RS23370"
                     /old_locus_tag="HV128_23385"
     CDS             join(3528..3786,3786..4648)
                     /locus_tag="HV128_RS23370"
                     /old_locus_tag="HV128_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="WP_085950818.1"
                     /translation="MKKRFSDEQIISILREAEAGVPARELCRKHAISDATFYTWRKKYG
                     GMEVPEVKRLKSLEEENTRLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCDAT
                     GLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRREGL
                     HVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALSTGRRI
                     KCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTCRALDQ
                     WAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRQDYNE
                     CRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
                     /name="IS3-like element ISSen4 family transposase"
     gene            4954..5187
                     /locus_tag="HV128_RS23375"
                     /old_locus_tag="HV128_23390"
     CDS             4954..5187
                     /locus_tag="HV128_RS23375"
                     /old_locus_tag="HV128_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(2453..2531)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          2607..3095
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(536..2031)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgacggcaag cctacagaat cagactcagg cctgctgggc tataaagacg caaatggtgt
       61 caaacagcag atcaataaag ccgacgttaa agaagtctct gaaattccgc attgaggcat
      121 cgctctctcc gaaaagggcc gctaatgcgg cccttttctt tatctgcccc aacaacaggc
      181 aaaaaaagcc cccgctgggg gccaaatacc tgcagtggct ggatagtact gcattcctga
      241 tgaaggagac gaataagtat gggccggatt tttgcgtctg caggctcggt cgccacaatt
      301 tttccgtacc cgccaggcat catgctttta ccataacatc ccttgcccgc acctctccgg
      361 acgggttcag cgcgaatgcg gcgtcagtcc tgcaaaaccg acagtaaacc accccgcaaa
      421 ccgtgcattc tgcgcctgat aaatctcacc cgactgcata gccatgcagc cgggtgaatg
      481 gcaccgggtt tcggcgcaga tcctcaatga aagccccgtt ttatccggtt atcacagcga
      541 cggaccgtca tactcctgct gacgctgcga tcgttccagc tccagcgctt tctggtgctg
      601 cgctgcgcgc tcctgctccc ggtgtgcctc gatgtcgttc agccttacga caaccggcgc
      661 agctgctcgc ttaaactcag cacctgcgtg ctcaagtccg tgacgcgctc gctcagcgct
      721 gcgttctccc gttgcataag accacacatc gtccgccatt ccgcgaaggc gctctcccac
      781 tcgtccagcc ttttcgagta gtcctgctgt agctgctcta atgcgctcag caactgtttt
      841 tccagttccg tcatgtgcta tttccccgcc agtatcatcc agctcgaatc ctctccgaac
      901 cgtcccgcgt ccgggttcac cctcacgcag cggcgtcgtt cctctccgca ggtcgtgagc
      961 tgcacgccgc tgttcctctc ggacagcatg gcctgcgtgc tcttctgctc ccggatggtc
     1021 gtgtaattgt cgagtatctg ctgcccctgc caccatagaa tgcccgcact tgaggctatc
     1081 agcagcacgg agaccaggac gatggtcagc cacgtctggc tgaccatgcg cagcatccct
     1141 ttcgtacgct ggctcatggc tgaggacagc atccggtcgt ggtcgtggat ggcggcgctg
     1201 attctcttct cgctttcgtt cagttccgct ctgacagact tctcgtgctc gctgaatgcg
     1261 gctttcagca tctcgccggt agtctgctgc tgcactttcg atttctgctc taagtccttc
     1321 gccagcgtta aaagactgtt catagatggc tcccttcagt cggatgtttc gccccccgtc
     1381 cgggtcggca atgctgatgc tgctttttgt ggtgcgaacc acctcaaaac ctgcgctttc
     1441 cagcgcctca gtgacgtcct gacgcgtttt cagctccccg gacgaggcta aggccagtaa
     1501 accccgcgta atcgcctgag cggcttcctg cttcgtttcc ggcagcgcgg acggcgtgac
     1561 cagtgcccgc cggttttccg gcgcgttcgg gtcgtgcagc cccagactgc cgttcacgat
     1621 ggtctgccag gcatcaatgc gaggacggtc ggcgcggtca tagtatggct ggagccgttt
     1681 gccggtcagc agctccgtgt tcgggatcag gaagttcagc tccagccgcc ccttgtcccg
     1741 gtgctcaacc cacagcacgc tgtactggtc tttatcgagt ccgggcatca gaacccgctc
     1801 gaagctcgcc atcagcgttt cacgctgccc gggcggtaag tcctgttcgg caaaggacag
     1861 caccccggag gtgtactttt tggcgtaggg cgaggcgtcg ataagctccc ggacttcctc
     1921 cggcttcccc tgaagaacgc tggcaccgtc gcgctggcgg tctttcccca gcagataatc
     1981 aaccgggccg gcaccgcctc cgcgtccccg gggatgaaac ttaacgatca tcgtcagccc
     2041 ccttttccag tacggcatgc cgcagccgct cgagtccggc atcgatggcc atcagcgcgg
     2101 cgacgacctg cacgcggtcg agaccgctac tgccaccggc gttaacctgg cgggcaatct
     2161 ggttgaggtt gttccccatg ccggcaagct gacgaagcag cgccggcgag atcgagggaa
     2221 gtttgccggc ccgggcgggc ttctcgtcca ggcacgtctg ccgcatccag gccgcgagct
     2281 gtttaccgtc gcagcgttcc agcagacggc ggtgctcatc ttctgttacc cacatcgtga
     2341 gcattttgct gcgtttgtct gccaatccgg cctcctgata acgccgggac gggcgggaaa
     2401 tcttttcccg gtcggcgcgg tgttgggcaa gccgcaggcg cgtcagtggc atttagcggg
     2461 tttcggggcg cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttacta
     2521 tggtggcacg acgggaatcg ggatgaaata gtcagtcatg aaaactgtga gatgaggctc
     2581 aggtgcgtaa gaggctgatg cctgtcccgg tggatattcc gcttcctcgc tcactgactc
     2641 gctacgctcg gtcgttcggc tgcggcgagc ggtacggact cacggataaa gacgtggcgg
     2701 ggaaatacgt gaatgcagga ggggaagaaa ggatgcgccg ctgccggttt tccataggct
     2761 ccgcccccct gacaagcatc acagaatctg acgctcaaat cagtggtggc gaaacccgat
     2821 gcggcttaaa gataccaggc gtttccccct gatggctccc tcgtgctccc ccgcattcct
     2881 gcctttcggt ttaccggtgt cattccgctg ttatggccgc ggtttctcat tccacgcctg
     2941 acactcagtt ccgcgaaggc agttcgctcc aggattgact gtatgcacga accccccgtt
     3001 cagtccgact gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacac
     3061 gaaaaatcgc tactggcagc agccattagt aactggaggt atacgagaga caaattaaga
     3121 ggtcttgaag tatgggcctg agagaggcta cactgaaaga acagttttgg tttctgcggt
     3181 ctcgtatacc cagttacctg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     3241 acctccccag gtggtttttt cgttttcaga gcaagagatt acgactgtaa cgacaggatc
     3301 acaagaagat catttactca gagagactaa ctaccactat gtttgcctat caacatactt
     3361 aggaaacatt acttgagtta acgactccac cttactttta gtatctcgca atgaatcttc
     3421 cgaccgccgt tgtgttgaag ttctattgcg ccaccgtgac ctgctccccg ttgattagta
     3481 caccccgatg ttagtaatgt cttcataagc cacatgagga catccccatg aagaagcgtt
     3541 tttccgacga acagatcatc agtattctcc gcgaagccga agctggggta cccgcccgtg
     3601 aactctgccg caagcatgcc atttccgatg ccacgtttta cacctggcgt aagaagtatg
     3661 gcggtatgga ggtgcctgaa gttaagcgcc tgaagtcgct tgaggaagag aacaccagac
     3721 tcaagaagct gcttgccgaa gccatgctgg ataaagaggc gcttcaggtg gctcttgggc
     3781 gaaagtactg acgacagacc agaagcggga agccgtgatg ttgatgtgtg atgcgaccgg
     3841 tctgtcgcaa cgtcgtgcct gcaggcttac aggtttatcc ctgtcgacct gccgctatga
     3901 ggctcaccgt ccggctgctg atgcgcattt atcagggcgc atcactgagc tggcactgga
     3961 gcgcaggcgt tttggctacc gtcgtatttg gcagttgctg cgccgtgaag ggcttcatgt
     4021 taatcataag cgcgtgtacc ggctttatca cctcagtggc ctgggcgtaa aacgcagaag
     4081 acgtcgtaaa gggctggcaa cagaacgtct gccgctgctc cgtccggcgg cgcccaatct
     4141 gacctggtcg atggatttcg tcatggacgc actttccacc ggtcgcagga tcaagtgtct
     4201 tacctgcgtc gatgatttca caaaggaatg cctgacggtc actgttgcct ttgggatttc
     4261 aggcgttcag gtcacgcgta ttctggacag cattgcactg tttcgaggct atccggcgac
     4321 gataagaact gaccaggggc cggagttcac ttgccgtgca ctggatcaat gggcctttga
     4381 gcatggtgtt gagttgcgct taatccagcc gggcaagcca acgcagaacg gatttattga
     4441 gagctttaac ggacgatttc gcgatgaatg tttgaatgag cactggttca gcgatatcgt
     4501 tcatgccagg aaaattatta atgactggcg gcaggattat aacgaatgcc gcccgcactc
     4561 cacgctgaat tatcagacac cgtctgaatt tgcagcgggc tggagaaagg gtcattctga
     4621 gaatgaagat tccgacgtta ctaactgagt gttgtatcta atcgtggggg caggtcaacc
     4681 ggggtataaa tcactttcgt ttaactcaat taaaagctcg ttcttgtgag catcctctaa
     4741 ataaaggtaa taacacccac cataaaggtt ctgcgtatct ggcacggcaa caaaagattt
     4801 ctttccgaac agtaaaacag cctctttttc tccatgctct gtcagcaata atcgaaattc
     4861 ttgtccattc aatcctcttt tcgcatgaaa aaacgaccta tacttagatg gttagtgacc
     4921 atatccactt tacctttaag cagggaacct attatgaata aaaaaacgct gttggcggcc
     4981 atgatggtcg cgttggtgac ctttgccgct gctggatgtt cttcaaacca agaaataaaa
     5041 accacagatg atcgcactat cgttacagac ggcaaaccca aagttgacag tgatacggga
     5101 ttagtatcct acaaagatgc ccatacaggc cgaaccgaac agatcaaccg tgacaaaatt
     5161 accaacatga gtgaactaga caactgatac ggataaaagg agctggcttt gaaaaagatt
     5221 tttgtttcag tgttcgcagc ggcagttgcg ctttcagccc taaccggctg cacgcgcaca
     5281 agctatgcca tccacaccaa cgacgggcgt acaattgtca g
//
