LOCUS       NZ_CP056750             2495 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00217 plasmid pRHBSTW-00217_4,
            complete sequence.
ACCESSION   NZ_CP056750
VERSION     NZ_CP056750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148546
            Assembly: GCF_013743755.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00217
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.499277906647x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013743755.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:08:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,389
            CDSs (with protein)               :: 4,389
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 30 of 83
            Pseudo Genes (incomplete)         :: 45 of 83
            Pseudo Genes (internal stop)      :: 23 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            Pseudo Genes (short protein)      :: 1 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056750.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00217"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00217_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            438..656
                     /locus_tag="HV129_RS22835"
                     /old_locus_tag="HV129_22865"
     CDS             438..656
                     /locus_tag="HV129_RS22835"
                     /old_locus_tag="HV129_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(947..1132)
                     /locus_tag="HV129_RS22840"
                     /old_locus_tag="HV129_22870"
     CDS             complement(947..1132)
                     /locus_tag="HV129_RS22840"
                     /old_locus_tag="HV129_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1498..1725)
                     /locus_tag="HV129_RS22845"
                     /old_locus_tag="HV129_22875"
     CDS             complement(1498..1725)
                     /locus_tag="HV129_RS22845"
                     /old_locus_tag="HV129_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          813..900
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..186)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.701"
                     /coverage="99.0"
                     /evalue="1.52e-53"
     Typing          complement(1961..2467)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
ORIGIN
        1 tgtcaggggg gcggagccta tggaaaaacg gctttggcgc ggccttatgc tttcttcgtt
       61 aagtatcttc ctggcatccc ccaggaaatt tctgatccat ccgtaagccc gtcccgctcg
      121 ccgcagccga acgaccgagc ggagcgagtc agtgagcgag gaagcggaat atattctgta
      181 tcacattttc tcctgacgcg ttttctttca ctttctgcgc ctgtcttatg tggcattaat
      241 gctatgtgtt actgccatgc tacatcttaa gccagtatac actccgctag tgctccgtga
      301 ctggtccggc gctgcgcccg gaacccgcct gtaccggttc agcagccgtt ccggcctgac
      361 tgcaattttt tttttttcat ccctgcccgc taccctgtaa acctttcttc tgcgttgccg
      421 ttaacagtag gtaagccatg tttggttttt cttccggagc cctgatgact cttagtgatg
      481 atgagcgaca cctgcttgtg tccgttgttt cggtctggct acggcgtgcc ggaggggatg
      541 ccggagccat gatgctggat gcataccggc agattttgtc tgaaaccgaa ccggccgtaa
      601 ggactgtcat gctcgaattt cttgaatccg ttcgcattca ttacatttcg tcctgagcat
      661 tttctgtatt tctgtccata cttaaacgag gcggccgtca cggacggtgg tcgtcgttcc
      721 atgatgctgt tatggacgag tgagagtaat ctaatctaac ttgattgagt tccgggcgtg
      781 ccgcccggtt ttttttgctg tttcccggtg tccgttccgg gacaggatgt gcatttgtaa
      841 taccgcgcac gcgccggcat cacaaatacc ctggtcaggg ctgcgccccg acaccccgcc
      901 atcgcctcac ggccgcgcac tgaattgcgc acccgttcac cgattttcac gcattttcct
      961 cgcctatctg tgcgttcagc atggcgtaaa gcgcttcggc ctgatcgtgc aggtgctcac
     1021 agcagccggc agcctcatcc agatccatct gctcgagccg ctcaaggagg agaagtgtct
     1081 gtgcgcggat aaatttggcc atattgaggg cggcggattc ctgtctggtc actggtgatt
     1141 ttccccgtct ggtgatgatt tttgcgtgaa aagagcatgg cgtcattctg gtgcatagtc
     1201 atgcatgccg ttaaaattta acaggcgcgt ttctgacggg ttccggggtg gtttgttgtg
     1261 gtttttgtca tggtttcgtc aggaacgcgc tgaacgcgtc tcaggcggtg cgggcaacgg
     1321 atgttatggt aaattcttgg gggcttgatt ataaaatacc gcacttcctc ttatgtcttg
     1381 tgctttttag acaaccctga aagcttttag ctttggtgat gcaatttgct gggattttgg
     1441 cggagaacca caggtaaaga aaaaggccac attagcggcc tttttcggag aagatgctca
     1501 gtgcggaatt tcggagactt ctttaacgtc ggttttattg atctgctgtt tgacaccatt
     1561 cgcgtctgta tagccaagca ggcctgagtc tgattcttta ggtttgccgt cgctgacaat
     1621 agtgcgaccg tcattggtgt gtatggcata gcttgtgcgc gtgcagccgg tgagggctga
     1681 aagcgcaact gccgctgcga acactgaaac aaaaatcttt ttcaaagcca gctcctttta
     1741 tccatatcag ttttggtcac taaccatcta actataggtc attttttatg tgaaaagagg
     1801 cttgatggtg ggctgtatat cgagttaggc gcgaggtgct atggtcaaag tgtggtgtca
     1861 ggcggggagg aggcttatat gagatgtaat ccggccgata catataccaa gatgatttaa
     1921 gtattcctct ttgtgaggtt ggatgtctaa tttataaagg atcttcttga gatccttttt
     1981 ttccgcgcgt aatctcttgc cctgtaaacg aaaaaaccac cctggcaggt ggtttttcga
     2041 aggttaggta atcctggcag atcccctaac cgtggtaaca gtcttgtgcg agacatgtca
     2101 ccaaatttgt cctttcagtg tagcctcact aaggccgcca cttcaagaac tcttgagaca
     2161 tctctcgcac atcctgtttg ccaatggccg ttgccaatgg cgattagtcg tgtctttccg
     2221 ggttggactc aagttgatag ttaccggata aggcgcagcg gtcggactga acggggggtt
     2281 cgtgcataca gtccatcctg gagcgaactg ccttcccgga actgagtgtc aggcgtggaa
     2341 tgagaaaccg cggccataac agcggagtga caccggtaaa ccgaaaggca ggaatgcggg
     2401 ggagcacgag ggagccacca gggggaaacg cctggtatct ttaagccgca tcgggtttcg
     2461 ccaccactga tttgagcgtc agattctgtg atgct
//
