LOCUS       NZ_CP056746             4783 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00220 plasmid pRHBSTW-00220_5,
            complete sequence.
ACCESSION   NZ_CP056746
VERSION     NZ_CP056746.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148549
            Assembly: GCF_013743295.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00220
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109.836668560382x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013743295.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:17:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,715
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,510
            CDSs (with protein)               :: 4,510
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 27 of 91
            Pseudo Genes (incomplete)         :: 60 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056746.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00220"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00220_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(325..648)
                     /locus_tag="HV132_RS23415"
                     /old_locus_tag="HV132_23430"
     CDS             complement(325..648)
                     /locus_tag="HV132_RS23415"
                     /old_locus_tag="HV132_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1299..>1421
                     /locus_tag="HV132_RS23780"
                     /pseudo=""
     CDS             1299..>1421
                     /locus_tag="HV132_RS23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1741..2097)
                     /locus_tag="HV132_RS23420"
                     /old_locus_tag="HV132_23435"
     CDS             complement(1741..2097)
                     /locus_tag="HV132_RS23420"
                     /old_locus_tag="HV132_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2300..2617)
                     /locus_tag="HV132_RS23425"
                     /old_locus_tag="HV132_23440"
     CDS             complement(2300..2617)
                     /locus_tag="HV132_RS23425"
                     /old_locus_tag="HV132_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2804..3046
                     /locus_tag="HV132_RS23430"
                     /old_locus_tag="HV132_23445"
     CDS             2804..3046
                     /locus_tag="HV132_RS23430"
                     /old_locus_tag="HV132_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(3717..3932)
                     /locus_tag="HV132_RS23500"
                     /old_locus_tag="HV132_23450"
     CDS             complement(3717..3932)
                     /locus_tag="HV132_RS23500"
                     /old_locus_tag="HV132_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3939..4430)
                     /locus_tag="HV132_RS23505"
                     /old_locus_tag="HV132_23455"
     CDS             complement(3939..4430)
                     /locus_tag="HV132_RS23505"
                     /old_locus_tag="HV132_23455"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     Typing          complement(764..937)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          861..1408
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(4..335)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.099"
                     /coverage="85.0"
                     /evalue="1.41e-66"
     Typing          complement(332..648)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
     Typing          complement(3626..4782)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.784"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtattgggga tcagaaagtt cagctccaga cgccccttgt ccgcgtgctc cacccacaga
       61 atgctgtact gatctttatc gagtccgggc atcagaaccc gctcgaagct ggccatcagt
      121 ttttcccgct ggccgggagg cagatcggcc tccgcaaacg acagtacgcc ggaggtgtat
      181 ttcttcacgt aaggcgacgc gtcaatcagc tcccggactt cttccggctt cccctgcagg
      241 acgctggcgc cttcacgctg ccggtcttta cccagcagat agtccaccgg cccggcgcca
      301 ccgccgcgac cgcgtggatg aaatttaacg atcatcgtcc gttcctttct caagaacggc
      361 gtgccgcagc cgctcaagcc cggcatcgat ggccatcagc gccgccacaa tctgtaccct
      421 gtcatggccg gtgccgccgc cggcgttaac acggcgggct atctggttca ggttattgcc
      481 cataccggca agctggcgca gcagcgccgg cgagagtgag gggagctttc cggtgcgcgc
      541 aggcttttcg tccaggcagg tctgccgcat ccacgccgcc agcggcctgc cgtcgcagcg
      601 ctcaagcagc cgccggtgct cgccctccgt cacccacatg gtgagcatct tgttccgttt
      661 ctctgacaaa ccggcctcct gataacgccg ggacgggcgg gaaagccttt cccggtcggc
      721 gcggggttgg acaagccgca ggcgcgtcag tggcttttgg cgggtttcgg ggcgcagccc
      781 tgaaccagtc acgtagcgct agcggagtgt atactggctt aatatgttgg cacggatacg
      841 gaaatcagtg aagtgcttca tgtggcagga gaaaaaaggc ggcaacggcg cgtcagcaga
      901 atatgcgata caggatatat tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      961 ggctgcggcg agcggtgcgg gcttacgaac ggggcggaaa tttcctggaa gatgccagga
     1021 agaaacttaa cagggaagcg ataaggccac ggcagagccg gttttccata ggctccgccc
     1081 ccctgacgag catcacagaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1141 ataaagatac caggcgtttc cccctgatgg ctccctcgtg cgctctcctg ttcctgcctt
     1201 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     1261 tcggttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1321 ccgaccgctg cgcctgttcc ggtaactatc gacttgagtc caacccggaa agacacgaca
     1381 aaacgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1441 ctacagagtt cttgaagtgg tggcctaact acggctacac tggaaggaca gtatttggta
     1501 tctgcgctct gctgaagcca gttaccaggt taagcagttc cccaactgac ttaaccttcg
     1561 aaaaaccgcc tccccaggcg gttttttcgt ttacagagca agagattacg cgtacagagc
     1621 acggatctca agaagatcgt aagaagaaag ttaacatgag cgtttttatc cctcaaaaag
     1681 aaggcttcta gttatagaag ccttcttttt gagggataac ttatattttt ttaaaaaaaa
     1741 tcatgcaata gcatgtttct tctcagaact atcaacaatg tgtttccttt ctatgatttc
     1801 atgagcggga atcactctgt ctatttcttc ttgcaatcga gggctactag taacctccca
     1861 taaatcaaca gactgctgta tatttagcca gaactttgca gtggtatcga aggctttagc
     1921 caacctaaaa gccatatcgg tagacagctt tctattattg ttaactaaag cactggcaga
     1981 gtttctatgc acattcaaca tttcagccaa gtcattgatt tttaaaccac ttggttcgag
     2041 atattcataa agaataatat cccctacagt tgatggtttt ctagttgctt gggacatgct
     2101 ttactcctaa attttttact tctctctgtt agatgaggaa gataagtatt tcgtctatta
     2161 ccagctgtag cgatgagtaa ttatcttaaa tatagtcacg tcattttttt tattaatcat
     2221 tatttgtaat gacattttaa tgtaggtgag ggaacgcctc acctacttgt tttataagat
     2281 aacagccacg acctgttatc tatcggtaat catgtggatc gagatacaaa tcctcgactc
     2341 cgttttcacc ccacttaaat atcaagcggt acttctcatt cacccgtatg gatgaatacc
     2401 ctgtcaatgg agggttgaga ttctcaaatc ggtttcccgg gggagaccgc aaatctccaa
     2461 ctttatcagc agcattcagc atatccagct tgcgacctaa tgaagcacta atcgcagttg
     2521 gaatctttcg attggcttta ccatatttgt aaaactggta tagccaatcg tctcgaaact
     2581 gaatgcggca atcatcatgt tcatgatctt gctgcatttt tcttttacct caatttttgc
     2641 cgcaacttgg caattttttc gatcaccttc ttacctccga tatagtacag caaacatatc
     2701 aaatgcacaa cagcacagtg cacttgtgca atcaatggct atgctgtaga atgcactcat
     2761 gcacagtgcg ggtgtgcaaa ataaatgata atttggaggt tgcatggctc ttgttcgctc
     2821 aggaaacaaa agcaagattt ttgaagcaga tacgaagttt aactcggttt ttgacactga
     2881 atataagccg ggagaaacat gtgaatatga agggatttat aaatgtaccg gttgcggtaa
     2941 agaagtaact atcgcaggcg ggaaaacaat cccaccacaa aaccaccacc aacatacccc
     3001 cgcgcaaggg aacattcgtt ggcaattgtt tgtttatgct cagtgaggta tagaaaatgg
     3061 cagatcgtcg accactcaac ccacacgttg tcgttcctaa accaggcggc ggaactcaac
     3121 ctcgtgaccg taacaatgac ggaagggtca gaggaaaacg ctcagatgca ggaaagccac
     3181 gaaaataaca gcaagccgcc agcctggcgg cttttttata tctgcagctg atactgattt
     3241 accataacat ccgttgggcg caccgctgaa acggggttgg ctgcgtttcc ggtgccagac
     3301 gcgagaaagt ggcgacagac ataacgaaaa aacaccagaa acgcagccgc tgaatattac
     3361 actggtgcat agtcatgcag ccagatgcat ggatgttaaa gtcacactaa aatccgcgcc
     3421 aatgccttaa aaaccgacaa atgcttctcc agccagtccc acagctccgc cgccagatac
     3481 aggcagttaa gcaatgattc aatgagattt aacagcttca tttcccgctc ctgaataagt
     3541 cgtgaccttt ttgatcacga acctatttag cgggagaaat aagagcccct cttcgtgcgg
     3601 gttgtcagct gcctggatgg tttaaagtgt cggcccgcgg taggttttct gcggctggcg
     3661 ctccagctcc agtgttttct gatactcatt ctccctctgc cggagctcga tggcattcag
     3721 ttcccggaca agcggcgcag ctgctcgctt aaactcagca cctgcgtgct caagtccgac
     3781 acgcgctcgt tcagcaccgc gttctcccgt tgcataagcc cacacatgct ccgccattcc
     3841 gcgaaggcgc tctcccattc gtccagcctt tgcgagtagt cctgctgtag ctgctctaat
     3901 gcgctcagca actgcttttc cagctctgtc attttccgct actcctgttt caggcggatc
     3961 catgtcgtcc cgtcgtaggg gatggcttcg gtttgcatcc cgggcggcag gataagaaac
     4021 ctccggccgt cgctgctctc ctgatagcgc actccccacg tccgggcagt cattttcgcc
     4081 agggtatctt cctgctgacg aagatgcgtg taattgtcgg ttatctgctg cccctgccac
     4141 cacagtatgc tcccgctcgt gccgatcagc agcgctgaca ccatcaggat ggtgaaccat
     4201 gtccgcccgg cagtccgcag tacgctccgc cggtttttct ccatcgcctc gctcatactc
     4261 tgctcgtgtg cgaggatggc gtcgctgatt ctcctcgcgc ttgcgctcag ttccgctctg
     4321 acagactgct cgtgctcgct gaatgcggct ttcagcatct cgccggtact ctgctgctgc
     4381 gctttcgatt gctgctctaa gtccttcgcc agcgttaaaa gactgctcat agatggctcc
     4441 cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatactgc ttttcgtggt
     4501 gcgcaccacc tcaaaacctg accgttccag cgcctcagtg acgtcctgac ggtttttcag
     4561 ctccccggac gaagcaaggg tcagcagtcc gcgcgtgatg gcttcagtgg cctcctgctt
     4621 cgttttcggc agtccggacg gcgtgaccag cgcccggcgg ttttccggcg cgttcgggtc
     4681 atgcagcccc agtctgccgt tcacgatggt ctgccaggca tcgatgcgcg gccggtcggc
     4741 gcggtcgtag tacggctgga gccgcctgcc ggtcagcagt tcg
//
