LOCUS       NZ_CP056745             4853 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00220 plasmid pRHBSTW-00220_4,
            complete sequence.
ACCESSION   NZ_CP056745
VERSION     NZ_CP056745.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148549
            Assembly: GCF_013743295.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00220
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109.836668560382x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013743295.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:17:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,715
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,510
            CDSs (with protein)               :: 4,510
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 27 of 91
            Pseudo Genes (incomplete)         :: 60 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056745.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00220"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00220_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3697..4853,1..367))
                     /locus_tag="HV132_RS23775"
     CDS             complement(join(3697..4853,1..367))
                     /locus_tag="HV132_RS23775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_181549197.1"
                     /translation="MRKERTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEE
                     VRELIDASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHA
                     DKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRAL
                     VTPSGLPKTKQEATEAITRGLLTLASSGELKNRQDVTEALERSGFEVVRTTKSSISIAD
                     PDGGRNIRLKGAIYEQSFNAGEGLRAAIESAAAEYRRDAESRIQRARAVCQSGTERKRE
                     ENQRRHPRTRAEYERGDGEKPAERTADCRADMVHHPDGVSAADRHEREHTVVAGAADNR
                     QLHASSSAGRYPGENDCPDVGSALSGEQRRPEVSYPAARDANRSHPLRRDDMDPPETGV
                     AENDRAGKAVAERIRAATAGLLAKAGRMGERLRGMAEHVWAYATGERGAERARVELEHA
                     GAEFKRAAAPFVRELNAIEEKAALEYQRQNELELTLRQKQKSFDGPKMR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(339..662)
                     /locus_tag="HV132_RS23390"
                     /old_locus_tag="HV132_23400"
     CDS             complement(339..662)
                     /locus_tag="HV132_RS23390"
                     /old_locus_tag="HV132_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181549196.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1648..2076)
                     /locus_tag="HV132_RS23395"
                     /old_locus_tag="HV132_23405"
     CDS             complement(1648..2076)
                     /locus_tag="HV132_RS23395"
                     /old_locus_tag="HV132_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            2361..2789
                     /locus_tag="HV132_RS23400"
                     /old_locus_tag="HV132_23410"
     CDS             2361..2789
                     /locus_tag="HV132_RS23400"
                     /old_locus_tag="HV132_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2846..3337)
                     /locus_tag="HV132_RS23405"
                     /old_locus_tag="HV132_23415"
     CDS             complement(2846..3337)
                     /locus_tag="HV132_RS23405"
                     /old_locus_tag="HV132_23415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     Typing          complement(778..951)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          875..1421
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.455"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(3..349)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.138"
                     /coverage="87.0"
                     /evalue="8.04e-70"
     Typing          complement(346..662)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="1.8800000000000002e-59"
     Typing          complement(3704..4851)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.371"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cggtcagcag ctcggtattg gggatcagaa agttcagctc cagacgcccc ttgtccgcgt
       61 gctccaccca cagaatgctg tactgatctt tatcaagtcc gggcatcaga acccgctcga
      121 agctggccat cagtttttcc cgctggccgg gaggcagatc ggcctccgca aacgacagga
      181 cgccggaagt gtatttcttc acgtaaggcg acgcgtcaat cagctcccgg acttcttccg
      241 gcttcccctg caggacggtg gcaccatcac gctgccggtc tttacccagc aggtagtcca
      301 ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt aacgatcatc gtccgttcct
      361 ttctcaagaa cggcgtgccg cagccgctca agtccggcat cgatggccat cagcgccgcc
      421 acaatctgca ccctgtcatg gccagtgccg ccgccggcgt taacgcggcg ggctatctgg
      481 ttcaggttat tgcccatacc ggcaagctgg cgcagcagcg ccggcgagat agaggggagc
      541 tttccggtgc gcgcaggctt ttcgtccagg caggtctgcc gcatccacgc cgccagcggc
      601 ctgccgtcgc agcgctcaag cagccgccgg tgctcgccct ccgtcaccca catggtgagc
      661 atcttgttcc gtttctctga caaaccggcc tcctgataac gccgggacgg gcgggaaagc
      721 ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcagtggctt ttggcgggtt
      781 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttattata
      841 ttggcacggg tgcgggaatc agtgaagtgc ttcatgtggc aggagaaaaa aggcggcacc
      901 ggcgcgtaag cagaatatgc gatacaggat atattccgct tcctcgctca ctgactcgct
      961 gcgctcggtc gttcggctgc ggcgagcggt gcgggcttac gaatggggcg gaaatttcct
     1021 ggtagatgcc aggaagaaac ttaacaggga aacgataagg ccgcggcgaa gccgtttttc
     1081 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     1141 aacccgacag gacttaaaga taccaggcgt ttccccctgg tggctccctc gtgcgctctc
     1201 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     1261 ccacgcctga cactcagttc cgggaaggca gttcgctcca agctggactg tatgcacgaa
     1321 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcaacttga gtccaatccg
     1381 gaaaaacacg acaaagcgcc actggcagca gccactggta acaggtttag aggaggagag
     1441 tcttgaagtt tcgggtgcga taaggctaca ctgaaaggac agttttggtg actgtgctcc
     1501 tccaagccag ttaccgcggt tcaaaagagc gtcaactctt gcgaaccttc gaaaaagcca
     1561 ccttgccggg tggttttttc gttttcaccg caagagatta cgacgagggt caacgaatct
     1621 caagggatca ttcatccgaa caagagatca ttcgaaattt tgggggagtt gcatagggtc
     1681 atgataccca agctctaaca gttttatatg gttcggcgct agacgttgga agtggatgac
     1741 ccaatcagag actttgtact tcccgtgaaa tgtatcgatg aaaacgggat cgcctatatg
     1801 ggcatgccac aatttattag ccctggcatg ttcttcaata gcttctttgt tttcgtaact
     1861 gtcaggcacg ttccaagatg gcgatacctt gccaatccaa gccggatggc cttttaaggc
     1921 atcgccaaaa agattgtact tctcgaaatg ttcgatgaag tcatcgattt tatcgagtgt
     1981 tttttgagaa aagcgggatg tgtaataaag attgaatgtc tcagagaaag agacaacatt
     2041 caattgtgga ttttgcgtgc ttgatccgct gcccatgctc ttctctgctc cctacttaga
     2101 ccagatggca ttgtaacaga tctctcattc gctctggaaa tcaacagaga caaggataca
     2161 ccaaatattt cagtatcttt tacaagatca tgatgttgtt tggttttcat atcactaact
     2221 ccgtatttaa tgcagataga aatgtttttc tgttctcaga ttagcctttt atagttgtgt
     2281 aataatggcg ctggtgtcac cctattcata tgaatactta tattatatat atctcaaaaa
     2341 aaatatttac aggataatca atgagttacg caatagaaaa aaaatggaag gctggaggtc
     2401 tgtcattagg atggactgca gtacctactg tgttgttttt tatacaaaat gagaagaaat
     2461 tgagccctgt agcattcaat accttgctga atctcatagt acactggtgg aatctacagg
     2521 agtggccgca cccctcaatg gagagcctgg ctatacggat gggggtgtca gtaaggacgg
     2581 tgcaaagggc tattaatgac ctagaaaaag ccaatctctt agataaaaaa ccgacatcaa
     2641 aaagcgacaa ccgatatggt ggccgcaaca tatacgacct tacaaagctt gtagattatt
     2701 tagacataat tggtccttct gtgtccgagc aagtaagaaa gccgcgacat aaaaagccaa
     2761 tatatacagt aaggaaaaaa gccgattagt tatgctggtt acatgcagga acagcccgca
     2821 tttggaggcc gggcttatcg ttaaattagg gcttaatcgt aagaccagaa cgttgaatgt
     2881 attcatcaac gcgatcgcca atgtttacaa ttttggcgaa attcacatca gccgatgaca
     2941 cgtttagatc cgttgcggat gcaggcatag gcaaggttgc acgatgacca tcgacggaaa
     3001 ccaaggtttt tctatcaaca aacgaagaac catagtgaac ggtgtactcg actgaacacg
     3061 cattactatc aggatgacga acagcccaag gctcattaaa cgagttgtag tcgtcgtaat
     3121 cagctctctt aatgtgcagt gctaaatcat ttttataagt aaacgcccct gtctcatcat
     3181 ccacaatcca atcattttga ctcgattgcg caagaattcg tgtaacttca tcagcattca
     3241 taaatagctc catcaggcag tgtgtagata aaaagcaaac aacatcacct acatgggtat
     3301 tatcagaacc tttatcaacg gacggttgat ttaccataac atctgttgcc cgcacagctg
     3361 aaacggggtt ggctgcgttt ccggtgccag acgcgagaaa gtggcgacag acatgacgaa
     3421 aaaacaccgg aaacgcagcc gctggatatt acacttatgc atagtcatgc agccagatgc
     3481 atgccgcgta aagtcacact aaaatcagcg cgaacgcctt aaaaaccggc aaatgcctct
     3541 ccggcaactc acacagctcc atcaccaggt aaaaacagtt cagcagggct tcaataacgt
     3601 taagaatttt catcacttca tccgggataa gtcactacca ttccggtagt cagcctattt
     3661 agcaggaacg cgatgagccc ttcttcgtgc gggctgtcac ctcatcttcg gaccatcaaa
     3721 actcttctgc ttctgacgga gcgtcagctc cagctcgttc tggcgctgat actccagtgc
     3781 agccttctcc tcgatggcat tcagttcccg gacaaacggc gcagctgctc gcttaaactc
     3841 agcacctgcg tgctcaagtt cgacacgcgc tcgttcagca ccgcgttctc ccgttgcata
     3901 agcccacaca tgctccgcca ttccgcgaag gcgctctccc attcgtccag cctttgcgag
     3961 tagtcctgct gtagctgctc taatgcgctc agcaactgct tttccagctc tgtcattttc
     4021 cgctactcct gtttcaggcg gatccatgtc gtcccgtcgt aggggatggc ttcggtttgc
     4081 atcccgggcg gcaggataag aaacctccgg ccgtcgctgc tctcctgata gcgcactccc
     4141 cacgtccggg cagtcatttt cgccagggta tcttcctgct gacgaagatg cgtgtaattg
     4201 tcggttatct gctgcccctg ccaccacagt atgctcccgc tcgtgccgat cagcagcgct
     4261 gacaccatca ggatggtgaa ccatgtccgc ccggcagtcc gcagtacgct ccgccggttt
     4321 ttctccatcg cctcgctcat actctgctcg tgtgcgagga tggcgtcgct gattctcctc
     4381 gcgcttgcgc tcagttccgc tctgacagac tgctcgtgct cgctgaatgc ggctttcagc
     4441 atctcgccgg tactctgctg ctgcgctttc gattgctgct ctaagtcctt cgccagcgtt
     4501 aaaagactgc tcatagatgg ctcccttcag tcggatgttt cgccccccgt ccgggtcggc
     4561 aatgctgata ctgcttttcg tggtgcgcac cacctcaaaa cctgaccgtt ccagcgcctc
     4621 agtgacgtcc tgacggtttt tcagctcccc ggacgaagca agggtcagca gtccgcgcgt
     4681 gatggcttca gtggcctcct gcttcgtttt cggcagtccg gacggcgtga ccagcgcccg
     4741 gcggttttcc ggcgcgttcg ggtcatgcag ccccagtctg ccgttcacga tggtctgcca
     4801 ggcatcgatg cgcggccggt cggcgcggtc gtagtacggc tggagccgcc tgc
//
