LOCUS       NZ_CP056736             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00316 plasmid
            pRHBSTW-00316_10, complete sequence.
ACCESSION   NZ_CP056736
VERSION     NZ_CP056736.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148572
            Assembly: GCF_013742375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00316
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.967439230937x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013742375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:50:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,917
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,694
            CDSs (with protein)               :: 4,694
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 36 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 24 of 107
            Pseudo Genes (multiple problems)  :: 17 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056736.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00316"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00316_10"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            350..535
                     /locus_tag="HV155_RS24445"
                     /old_locus_tag="HV155_24455"
     CDS             350..535
                     /locus_tag="HV155_RS24445"
                     /old_locus_tag="HV155_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2252..2479
                     /locus_tag="HV155_RS24455"
                     /old_locus_tag="HV155_24460"
     CDS             2252..2479
                     /locus_tag="HV155_RS24455"
                     /old_locus_tag="HV155_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(583..670)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1309..1837
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 aggccgctaa tgtggccttt ttctttacct gtggttctcc gccaaaatcc cagcaaattg
       61 catcaccaaa gctaaaagct ttcagggttg tctaaaaagc acaagacata agaggaagtg
      121 cggtatttta taatcaagcc cccaagaatt taccataaca tccgttgccc gcaccgcctg
      181 agacgcgttc agcgcgttcc tgacgaaacc atgacaaaaa ccacaacaaa ccaccccgga
      241 acccgtcaga aacgcgcctg ttaaatttta acggcatgca tgactatgca ccagaatgac
      301 gccatgctct tttcacgcaa aaatcatcac cagacgggga aaatcaccag tgaccagaca
      361 ggaatccgcc gccctcaata tggccaaatt tatccgcgca cagacacttc tcctccttga
      421 gcggctcgag cagatggatc tggatgaggc tgccggctgc tgtgagcacc tgcacgatca
      481 ggccgaagcg ctttacgcca tgctgaacgc acagataggc gaggaaaatg cgtgaaaatc
      541 ggtgaacggg tgcgcaattc agtgcgcggc cgtgaggcga tggcggggtg tcggggcgca
      601 gccctgacca gggtatttgt gatgccggcg cgtgcgcggt attacaaatg cacatcctgt
      661 cccggaacgg acaccgggaa acagcaaaaa aaaccgggcg gcacgcccgg aactcaatca
      721 agttagatta gattactctc actcgtccat aacagcatca tggaacgacg accaccgtcc
      781 gtgacggccg cctcgtttaa gtatggacag aaatacagaa aatgctcagg acgaaatgta
      841 atgaatgcga acggattcaa gaaattcgag catgacagtc cttacggccg gttcggtttc
      901 agacaaaatc tgccggtatg catccaacat catggctccg gcatcccctc cggcacgccg
      961 tagccagacc gaaacaacgg acacaagcag gtgtcgctca tcatcactaa gagtcatcag
     1021 ggctccggaa gaaaaaccaa acatggctta cctactgtta acggcaacgc agaagaaagg
     1081 tttacagggt agcgggcagg gatgaaaaaa aaaaaattgc agtcaggccg gaacggctgc
     1141 tgaaccggta caggcgggtt ccgggcgcag cgccggacca gtcacggagc actagcggag
     1201 tgtatactgg cttaagatgt agcatggcag taacacatag cattaatgcc acataagaca
     1261 ggcgcagaaa gtgaaagaaa acgcgtcagg agaaaatgtg atacagaata tattccgctt
     1321 cctcgctcac tgactcgctc cgctcggtcg ttcggctgcg gcgagcggga cgggcttacg
     1381 gatggatcag aaatttcctg ggggatgcca ggaagatact taacgaagaa agcataaggc
     1441 cgcgccaaag ccgtttttcc ataggctccg cccccctgac aagcatcaca gaatctgacg
     1501 ctcaaatcag tggtggcgaa acccgatgcg gcttaaagat accaggcgtt tccccctggt
     1561 ggctccctcg tgctcccccg cattcctgcc tttcggttta ccggtgtcac tccgctgtta
     1621 tggccgcggt ttctcattcc acgcctgaca ctcagttccg ggaaggcagt tcgctccagg
     1681 atggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     1741 caacttgagt ccaacccgga aagacacgac taatcgccat tggcaacggc cattggcaaa
     1801 caggatgtgc gagagatgtc tcaagagttc ttgaagtggc ggccttagtg aggctacact
     1861 gaaaggacaa atttggtgac atgtctcgca caagactgtt accacggtta ggggatctgc
     1921 caggattacc taaccttcga aaaaccacct gccagggtgg ttttttcgtt tacagggcaa
     1981 gagattacgc gcggaaaaaa aggatctcaa gaagatcctt tataaattag acatccaacc
     2041 tcacaaagag gaatacttaa atcatcttgg tatatgtatc ggccggatta catctcatat
     2101 aagcctcctc cccgcctgac accacacttt gaccatagca cctcgcgcct aactcgatat
     2161 acagcccacc atcaagcctc ttttcacata aaaaatgacc tatagttaga tggttagtga
     2221 ccaaaactga tatggataaa aggagctggc tttgaaaaag atttttgttt cagtgttcgc
     2281 agcggcagtt gcgctttcag ccctcaccgg ctgcacgcgc acaagctatg ccatacacac
     2341 caatgacggt cgcactattg tcagcgacgg caaacctaaa gaatcagact caggcctgct
     2401 tggctataca gacgcgaatg gtgtcaaaca gcagatcaat aaaaccgacg ttaaagaagt
     2461 ctccgaaatt ccgcactgag catcttctcc gaaaa
//
