LOCUS       NZ_CP056735             3726 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00316 plasmid pRHBSTW-00316_9,
            complete sequence.
ACCESSION   NZ_CP056735
VERSION     NZ_CP056735.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148572
            Assembly: GCF_013742375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00316
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.967439230937x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013742375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:50:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,917
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,694
            CDSs (with protein)               :: 4,694
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 36 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 24 of 107
            Pseudo Genes (multiple problems)  :: 17 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056735.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00316"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00316_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(1023..2153)
                     /locus_tag="HV155_RS24430"
                     /old_locus_tag="HV155_24440"
     CDS             complement(1023..2153)
                     /locus_tag="HV155_RS24430"
                     /old_locus_tag="HV155_24440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181542275.1"
                     /translation="MEFKIKKMTVKDSREIKKRFEDCIKIAEEKNMQVGSILNIEMQDR
                     LKEINLKVKKETAEQYRKLFNYKLSVNAEDIIQHLLECNTRNSFDKTRTAFRFCIAEKI
                     QELRNEADRARKQKNFDLMKEKTIEAYKLFFFFERDFLSENRVTWNDISYRKIESKSKK
                     KTMNSVSSIKAIFEDLKQKPELFDKYSMILAISSLTGCRPAEIQKGIGLELSPPLLNIC
                     ISGAKVGEDRGQEKRILKFNINEFAENEQFKLIMSKIKQNNKLEYKCSKQEYDSLRQYL
                     YINHKGFSLYTLRHRAASELKKEGFTEETIAAFLGHRVTRSLENYGYARSGKGGPAVAG
                     VEHSNAIKSNTKRFEKKTNPGGVNPTPTRKRALKRF"
                     /name="hypothetical protein"
     gene            complement(2526..3227)
                     /locus_tag="HV155_RS24435"
                     /old_locus_tag="HV155_24445"
     CDS             complement(2526..3227)
                     /locus_tag="HV155_RS24435"
                     /old_locus_tag="HV155_24445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139497357.1"
                     /translation="MTISKKNSELRERFKEYASNKNIFDLADLRYEILKIYCDFKLKSD
                     MSEKERKSQDSRRKAHLTALKKRIKREIVSKIVIDLVKYYNIEKTTFHFFSHICTEILE
                     RNVDNRYILNNFSNMILDEKKELRKLSESRNASNKMSLENSYSELVSMSHIKDKLFRND
                     NFKTAYLKCYGCANEEFSRFKVFAFPDNYETLDFLFEEERIKKEEKEISKIMIEQVEEE
                     QKIQPSKKRRL"
                     /name="hypothetical protein"
     gene            complement(3293..3541)
                     /locus_tag="HV155_RS24440"
                     /old_locus_tag="HV155_24450"
     CDS             complement(3293..3541)
                     /locus_tag="HV155_RS24440"
                     /old_locus_tag="HV155_24450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_073568787.1"
                     /translation="MEIEKLIDRYFLALERKVIREIYNKHTREELEKFIFKCNSEFREF
                     EHRFNTICSTEYYEQQSLIFYINYINAEFEQREKLNY"
                     /name="hypothetical protein"
ORIGIN
        1 cctttgtgtt cagcccttta cagggcttca cccaaaggtc tcccctagta aaaattttct
       61 ggatctaccc taaaaatcag gtaattagaa aattttcact aagggcgatt tgtgtgattg
      121 caaagttttg gacttcacca caaagttcta gactattgag cttaacaacc gtttccagtc
      181 gttggcaccc tcttacaccc tctagataca gttcatagcc ttttacttgc gacagctaac
      241 cccattttaa acaaccattt ggctgttatg gctgggcttt ctcatagcct accaatcaat
      301 cactgttcac caaaaaaacc ttagaactcg cctcccaggt cggcaattcg taagcaccct
      361 tctattgcca acaaccaacc gaaaccggac cctcttgatt ccacagtttc aggcacgaca
      421 caatatgcgc atttggttgc ttaaggattt gaaaaactag ggatttttga agagacttgt
      481 aacaagagag gtttgacaat tgcgatttta taagttagat tttcacttac atatcgtaat
      541 ccagtgcctg atatcacttg attacaagac tttcagaagt ccccacttct ttaagtccct
      601 caaaaccagc ttaggctggt tttgttgtat ctggactcag ttataatcta tctttctgtt
      661 atgtcaacac ttcttcaaaa aatttgattg ctcaaaaatc aaaattctca ctcaatgaga
      721 ctcgttgtgt catttttaac agcaaaaatg acacttcaaa tgccaataac tgacagttaa
      781 ttgatcaaaa aaccaccaac gaaaagttac cggggtcccg ttccgggcac tactcgcgcg
      841 cgtgccgcct gctaacacat atattgatta gttaattaca ccgaccacac aacgaaccac
      901 accgatctgt ggttctgcta atacacatat gcaaccagcc ggcacagatc tctgctggca
      961 agacgcgcgc gcgaggctaa cacacatatg agggatcagc attgctgacc cctggtaact
     1021 ctctaaaatc gttttaaggc tcgtttacga gtaggagtag ggtttacccc tcctgggttg
     1081 gttttcttct caaatcgctt tgtattgctt ttaatcgcgt ttgagtgctc aacacctgcc
     1141 acagccggac cacctttgcc agatcttgca tagccgtaat tttccagaga tctagttact
     1201 ctgtgaccca aaaatgcagc tattgtttct tctgtaaaac cctctttttt taattcgctc
     1261 gctgctctgt gtcttaatgt ataaagtgaa aaacctttat ggttgatata caaatattgt
     1321 ctcaatgaat catactcttg ctttgagcat ttatattcta atttattatt ttgctttatt
     1381 ttggacataa tcaatttgaa ctgctcattt tcagcaaatt catttatatt gaatttcaat
     1441 attctttttt cctgccctcg atcttcgccc actttcgccc cgctaataca tatatttaat
     1501 agtggtgggc ttagttccag cccaattcct ttttggatct cagccggacg gcaaccggtc
     1561 aaacttgaaa ttgctaaaat cattgaatac ttatcaaaaa gctctggctt ttgcttcaaa
     1621 tcttcaaaaa ttgctttgat acttgaaaca ctattcatag tctttttttt gcttttagat
     1681 tctatttttc tgtaagaaat atcattccaa gttacgcgat tttcgctcaa aaaatctcgc
     1741 tcaaaaaaga aaaacaactt atatgcttca atggtttttt ctttcattaa atcaaaattc
     1801 ttttgttttc ttgctcgatc tgcttcgttt cttagctctt gtattttttc agctatacaa
     1861 aatctaaaag cagttcttgt tttatcaaaa ctgtttcttg tgttgcattc taacaagtgt
     1921 tgaataatat cttctgcatt cacagacaat ttataattaa acaattttct atattgttct
     1981 gctgtttctt tttttacttt taaattaatt tctttcaatc tatcttgcat ctcaatattt
     2041 aaaattgagc ccacttgcat atttttttct tctgctatct taatgcaatc ttcaaatctt
     2101 tttttaattt ctctgctgtc tttaacagtc attttcttta ttttaaattc catagtcacc
     2161 tcattttttt atagtttttt ttgttttaat tttactccgc aggtttgcct tttttcttca
     2221 ttccaaatta aaaattttca aatttcgctt cgctcaaaat gaaatatttt taaaatgtca
     2281 ttacgaaaaa aagcaaaggg gttttgtgct cttcgcacac ttttcctttc aaaatttcgc
     2341 tgacgctcat ttttcaagta aaagacaacc ccccattatt gttataacaa aaataacaat
     2401 aatggcaagt caaaagaaaa aatagatcgc agattttact agatttttca tcaacttaca
     2461 cacagatcaa tcgctcaaaa aacaaaatta aaattttatt tttttcacga ttcgccgcaa
     2521 aaaaattata gtcttctttt tttacttggc tgtatttttt gttcttcttc aacttgttca
     2581 atcattattt tagatatttc tttttcttct ttttttattc tttcttcttc aaataaaaaa
     2641 tctaatgtct cataattatc cggaaaagca aaaactttaa atctcgaaaa ttcttcattt
     2701 gcacaaccat aacattttaa atatgcagtt ttgaaattgt cattcctaaa taatttgtct
     2761 ttaatatgtg acatagaaac aagttcggaa taagagtttt ctaaactcat tttgtttgaa
     2821 gcatttctgc tttcacttaa tttcctcaac tctttttttt cgtctaaaat catgtttgaa
     2881 aaattattta gaatgtatct gttatctaca tttctttcta aaatctcagt acatatatga
     2941 gaaaaaaaat gaaaagtcgt tttttctata ttgtaatact tcactaaatc aataactatt
     3001 ttcgaaacaa tttctctttt aattcttttt tttaatgctg ttaaatgtgc ttttcttctg
     3061 gagtcttgag attttctttc tttctcactc atatcgcttt ttaatttgaa atcacaatat
     3121 atttttaata tttcatatct caaatcagca agatcaaata tgtttttatt actggcgtat
     3181 tctttaaatc gctctctcaa ttcactattt ttttttgata ttgtcatata ttaagccctc
     3241 tttattttgc taatacatat atgcttaata tattatttta atattgtcaa atttaataat
     3301 ttaacttctc cctctgctca aactctgcat ttatataatt aatataaaag ataaggcttt
     3361 gttgttcata atattcagta gagcagattg tattaaatcg gtgttcaaac tctctaaact
     3421 cactattaca cttaaaaata aatttttcta attcttctcg tgtatgttta ttataaattt
     3481 cacggatcac ttttctttct aatgctaaaa aatatctatc tatcaatttt tctatttcca
     3541 taaattttcc ttttttaatt atatttagaa tttaaatagc aaattgcaaa aaaaaatcaa
     3601 atcgccaatt tttttcttcg aactctattt ttttattctt tagtattggt tttctatctt
     3661 tctacaaaga agcattgctt tttctctata acagatctgt attgttttgc tgtggcttcg
     3721 ctaaga
//
