LOCUS       NZ_CP056734             3813 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00316 plasmid pRHBSTW-00316_8,
            complete sequence.
ACCESSION   NZ_CP056734
VERSION     NZ_CP056734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148572
            Assembly: GCF_013742375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00316
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.967439230937x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013742375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:50:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,917
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,694
            CDSs (with protein)               :: 4,694
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 36 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 24 of 107
            Pseudo Genes (multiple problems)  :: 17 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056734.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00316"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00316_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            233..394
                     /locus_tag="HV155_RS24410"
                     /old_locus_tag="HV155_24420"
     CDS             233..394
                     /locus_tag="HV155_RS24410"
                     /old_locus_tag="HV155_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071784511.1"
                     /translation="MQEPPVQPDRCAFSGNYRLESNPERHDLTPLAAATGKQDVREIYE
                     EFLKWWPD"
                     /name="replication initiation protein"
     gene            complement(1193..1510)
                     /locus_tag="HV155_RS24415"
                     /old_locus_tag="HV155_24425"
     CDS             complement(1193..1510)
                     /locus_tag="HV155_RS24415"
                     /old_locus_tag="HV155_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225865423.1"
                     /translation="MVDLLAVDVCSYGEIVATDAQVKQINALIKKYPDSCSICHQEYDE
                     DYVTYTVFGYDKNQKMQVTTGCCARTLTELVLLGVCGCFDPDERDEIMENHPMAKKFFS
                     N"
                     /name="hypothetical protein"
     gene            complement(2266..2667)
                     /locus_tag="HV155_RS24420"
                     /old_locus_tag="HV155_24430"
     CDS             complement(2266..2667)
                     /locus_tag="HV155_RS24420"
                     /old_locus_tag="HV155_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_070585248.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESQAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2667..2858)
                     /locus_tag="HV155_RS24425"
                     /old_locus_tag="HV155_24435"
     CDS             complement(2667..2858)
                     /locus_tag="HV155_RS24425"
                     /old_locus_tag="HV155_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_070585250.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDGLQDDN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3481..3695)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.105"
                     /coverage="100.0"
                     /evalue="7.539999999999999e-48"
     Typing          2..380
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.712"
                     /coverage="87.0"
                     /evalue="7.63e-102"
ORIGIN
        1 gcaaagccgt ttttccatag gctccgcccc ccctgacaag catcacgaaa tctgacgctc
       61 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggtgg
      121 ctccctcttg cgctctcctg ttcccgtcct gcggcatccg tgtagtggtg gaagctttac
      181 ctaaatcacc acgccccgtt ccgtttagac agttcgctcc aagctgggct gtatgcaaga
      241 accccccgtt cagcccgacc gctgcgcctt ttccggtaac tatcgtcttg agtccaaccc
      301 ggaaagacac gacttaacgc cactggcagc ggccactggt aaacaggatg tgcgagagat
      361 ttacgaagag ttcttgaagt ggtggccaga ctgaggctac actaaaagga cagatttggt
      421 gacgtctcgc acaagactgt taccacggtt cagaaacttg ccggtttact gaaccttcga
      481 aaaaccaccc tgcaaggtgg ttttttcgtt tacatggcaa gagactacga cgtccaacgt
      541 cagatctcaa gaaattattt aaaaataaat agttaaccaa tatccttgtg atatttatta
      601 tgtttccttt atactcaaat aacatcaata ttcaggattg gaacactcaa acaagttaat
      661 acaacgatgt tctaggttca tatccctgag acttaaagtc taactagtat atttccgaac
      721 aagatcacta tcattccgta cttttttaaa taggtttatt taatgccata catatacgca
      781 ataatactaa caacttgttt taacacgaat aatattgcaa gttgttcttc agaattagca
      841 aatttgggaa tgaaagacaa tgttgttaac accttcattc tgaatgaaga atgcattgaa
      901 tttaaagata aaatcattgc aaacaaagac atttatcata atatatccgg gaaaaacata
      961 atatctgctg aatgtgtacc cgtatctatc cccaaatata ttttagacaa ttacagcaaa
     1021 caacaacgat aatgaatacc ccctctgcaa gcagcggggt atcaacacca aagatcaaat
     1081 acagagtagt tcgctccaag ggacggggaa tattgccttt agagatttac cataacgtct
     1141 cattgcacga acccaaacgc ccgcaaagag cgggcttcgg taatagtcag gtctagttgc
     1201 tgaaaaattt cttcgccatt gggtgatttt ccatgatctc gtcacgctca tctggatcaa
     1261 agcatccgca aacacctaac aacaccaatt cggtaagcgt tcgcgcgcaa catccagttg
     1321 taacctgcat cttctggttt ttgtcatatc cgaaaaccgt atacgtaacg taatcctcat
     1381 cgtattcctg atggcaaata gagcacgagt cagggtattt tttgatgagc gcattaatct
     1441 gttttacttg tgcatctgta gccacaatct ctccataaga acaaacatca acagccagca
     1501 aatctaccac atcagcttcg cgctaccgat ttaccataac gtcgcattac gcgcacctct
     1561 cagacagcct gagagcgttt ctggcagaaa accggtaaaa caggcaacaa accacccgaa
     1621 aacccgccag agacgcgcag gcgctatttt tacggcatgc atacgtatgc gcgcaagtgc
     1681 atagcccgaa aattcacgcc attcagccag ggtaagtcct gtttccggcg ttcttcccat
     1741 ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc
     1801 cggaaaaccg gttttcagca cgatcaggaa tcgggttcgt cgacggccgg caatcacgtt
     1861 atggcaagtc gacggacgac ttaccacacg attcaccggc gattcgactc gtgacttgac
     1921 tctagagtcg ccaaggtcaa aaaaacacca aaatgacgat tcgactctag agtcaagtcg
     1981 tcataagtta ctgatagtct tatgtgagtc gtcgatgact caatgacgag tcgtcggcaa
     2041 gtcgtacacg aatcgccgac gacttggatt taccttacag aaggtaataa ttcgtaattt
     2101 aataacacct aacaaactga tattaatgag ttatctgcaa aattttaatt ttgcgtggtg
     2161 tgtgggtatt ttaagtggtg aggtgcgttc agagatctga cgatttttgg gaaggttcag
     2221 gaactccccg gaatggccgg agaggaaaac tgaagcatga cggtgtcagg attttttcca
     2281 gaatttccac catggcgcct gggattcagt taattttgcc tggtgatttt caagaaggag
     2341 catggcctgt ttcaggctgt caatgtgcga atccttagac aaaagtaagg tgttcaggtg
     2401 ttcaatctcc tgtttcagat gacgaatcac ctgttcactc tggtcagcag aggctgtatc
     2461 acgctgttca gagacctgtc cagaatggtg ttcacccttc agttgttcaa agggaccaaa
     2521 gacacgtaaa agctctgaag tatctaccat ttttgcatta cgttcaccgc tacaggtgga
     2581 cagatcacct gctttaatta acctgtgcag tgttgttcta ctcttacccg tcagtcgggc
     2641 agcctcacta atagacactc tggccatcag ttatcatcct gtaatccatc cagtctcgag
     2701 gacagtgtac ggaaaagatg ctccgcatca tcgtggagtt tctcacaaag gtccgcctca
     2761 acatcgagat caagctcgtt aagcttctcc agcaggagca gagactgatt ctggatgaat
     2821 ttagccatgt tcagcgctgt tttctgttgt ttcgtcataa gggccctcag agcatcagtg
     2881 ctcagtgtaa accggtaagg gggtcggatg tacgaatggt tcagagaacg gatctgaggt
     2941 tatcctgtag ttattttgta gtttatctgt agtcatcgca ctaaaacgca ggattaaatc
     3001 atcaactttt gtagtcttcc tgtagtttac ctgtagtctg gaaaaataat ccgttcatgc
     3061 ttgcatgaac gaagcagtta aagacgcttt ttcgtcgccg atgatcctta acctgcgcag
     3121 atcacccagc cagaatgata attttttgcc ccttaaatgg tcatacacct aacgcagccg
     3181 cgatttttat cgcaactgca tgaattaaat gaaaaaaatg gagttacttc gtgtgttagc
     3241 gtaatacgca acgtattaca atcatctgta acaacatgaa aataaacaaa taacatgtaa
     3301 taccagcgta ttacatgtac gctggccagc gaaaaagcta taacgcttcc cggccgacag
     3361 gcgggccaga atccaccggg gcgtgcaggt tctcttcagt aacatgagcc attaagtcaa
     3421 aaaggtcata ttcgccggga aacagagcat cagtcgcatc cggttgttca tcatactcag
     3481 atggcatggt gtgatatcca atagctgagg ggtgttagcg ggtttcgggg cgcagccctg
     3541 aaccagtcac gtagcgctag cggagtgcat actggctaaa tattttaaac cgaagtggtg
     3601 atttgccaaa gtgcttcatg tggcaggagt gaaatgaggc tttccggcgt caggagaaga
     3661 tgctggttca ggtgatattc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3721 ctgcggcgag cggtgcaggc ttactaatgg ggcggaaatt tatttcctag tggatgccag
     3781 gaagaaactt aacagggata cgataaggcc gcg
//
