LOCUS       NZ_CP056732             5299 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00316 plasmid pRHBSTW-00316_6,
            complete sequence.
ACCESSION   NZ_CP056732
VERSION     NZ_CP056732.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148572
            Assembly: GCF_013742375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00316
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.967439230937x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013742375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:50:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,917
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,694
            CDSs (with protein)               :: 4,694
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 36 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 24 of 107
            Pseudo Genes (multiple problems)  :: 17 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056732.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00316"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00316_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4386..5299,1..22)
                     /locus_tag="HV155_RS24340"
                     /old_locus_tag="HV155_24350"
     CDS             join(4386..5299,1..22)
                     /locus_tag="HV155_RS24340"
                     /old_locus_tag="HV155_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_072206570.1"
                     /translation="MATYHLSVKFGGKGKALAHANYITREDKFSQRKDLEHTEHGNMPE
                     WAKDDPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLELVRDFVKQETGGQHTYS
                     FAIHNPQASIDGGEQPHAHIMMSQRVDDGIKRSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKAQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            25..555
                     /locus_tag="HV155_RS24345"
                     /old_locus_tag="HV155_24355"
     CDS             25..555
                     /locus_tag="HV155_RS24345"
                     /old_locus_tag="HV155_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            946..1245
                     /locus_tag="HV155_RS24350"
                     /old_locus_tag="HV155_24360"
     CDS             946..1245
                     /locus_tag="HV155_RS24350"
                     /old_locus_tag="HV155_24360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023281092.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHPEALFLASAVARDTFLKLIRERLCGGEDVDSWYGFQRNISNDHS"
                     /name="hypothetical protein"
     gene            1316..2038
                     /locus_tag="HV155_RS24355"
                     /old_locus_tag="HV155_24365"
     CDS             1316..2038
                     /locus_tag="HV155_RS24355"
                     /old_locus_tag="HV155_24365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181542273.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLENYFFRQGFDIDTGSYIDLI
                     CRDKESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIE
                     ETSILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNSNGNGREVSLVNGGFKRFITGDFM
                     PTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKKEAESRSGKWTLSNVKIEY
                     QFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     gene            complement(<2312..2392)
                     /locus_tag="HV155_RS24730"
                     /pseudo=""
     CDS             complement(<2312..2392)
                     /locus_tag="HV155_RS24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2826..2921
                     /locus_tag="HV155_RS24360"
                     /old_locus_tag="HV155_24370"
     CDS             2826..2921
                     /locus_tag="HV155_RS24360"
                     /old_locus_tag="HV155_24370"
                     /inference="COORDINATES: protein motif:HMM:NF013937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_249415292.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLPRRTSPLKIL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            <2882..3616
                     /locus_tag="HV155_RS24365"
                     /old_locus_tag="HV155_24375"
                     /pseudo=""
     CDS             <2882..3616
                     /locus_tag="HV155_RS24365"
                     /old_locus_tag="HV155_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3810..4208)
                     /locus_tag="HV155_RS24370"
                     /old_locus_tag="HV155_24380"
     CDS             complement(3810..4208)
                     /locus_tag="HV155_RS24370"
                     /old_locus_tag="HV155_24380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021526524.1"
                     /translation="MRISNIEWLKKRLGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(2266..2650)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.487"
                     /coverage="89.0"
                     /evalue="2.97e-154"
     Typing          2919..3441
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.092"
                     /coverage="92.0"
                     /evalue="0.0"
     Typing          4387..5297
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cggattttcc ataagcaggt aactatggcg agcaccgaag aaaaacttct cacactcctg
       61 ctgtcggcgg agcatctgaa agaggccgca aaagagcagc agaaagccgt ggcggattcg
      121 ctggcggtgg caagggaaac cctgaaaggc taccgggaaa tatcagacag tgtggcacag
      181 caggtacggg atgaagtccg gcaggaagtg cagaaaatgg atgttgcggg gctgattggc
      241 gagcgtatca gcgctgattt tcagcgtctg gaaaaatccg tcgggaacat gaaaatcaat
      301 gcggacgcgc tggacaagga aatttccatt atccgcagca atatcatcgc ggagtacaac
      361 acgctgcggg gttacagctg gaagtggttt gcaggggttg cggttgcctt tgtcgtactg
      421 atatttgcca caagctggtt tgtgaaaacg gaactacagg acaattacaa cgcgtctgct
      481 gccgtctatc agaaagtggc agaccttgaa aagctgctga caacggaaca gaaaccggaa
      541 aagaaaaaga actgacgcgc tgcgcttgtc caacgcctca cgttcgcaca ataaattgcg
      601 cacccgttca ccgttttcag ccgggtttgg ggcgtctttt gagacttccg gcctgaattc
      661 aggccggaag ttctcgccag tcagtcaggt gcatggttat gcatggggct gaaaatcacg
      721 gtatgcgatt ctgagcggtt ttcggtgaga agacgggggt ttttatggct caggacgtaa
      781 aaccgccctg agcgcgtttc agcggtgcgc gtaatgggac gttatggtaa atgcctgtct
      841 tgctcacttt tagagcatga ggcaaaaaag gtaaaaactt atttgcttaa cagcccctat
      901 cactatgtaa attaactcct gaaaactaaa aaatggagga ttggcatggc gactgctact
      961 aattttgatg cgtggttaga tgatgttgat ggcgattatg aggaggttat ggctctttat
     1021 gattccgttc agaatgtaag tgatatgggg ttgtatcagt gtgttgaggg tggtcggggt
     1081 gatgcttggg tcgtgagctc taatcatcat ccagaagctc tttttctagc atcagctgtg
     1141 gctagggata ctttcctcaa attgattaga gaaaggctat gtggcggcga agatgttgat
     1201 tcatggtatg gatttcaacg aaatatatcg aatgatcact cgtaatcgta cagagagttc
     1261 cccttgtggg aactcttaaa atatgacgtt atagtaaata ggatgcgtag gggttgtggc
     1321 tcttatggtg acgagaaggt acttactagg tggtgaatat ccctccgagg aatttgttca
     1381 agcttcttta gaaaattatt tttttcgaca agggttcgat atagatacgg gttcttatat
     1441 tgaccttatt tgtcgtgata aagaatctag ggatgttgtg tggcatattg aagtaaaagg
     1501 aaaaacatca cagccagggt tggattttcg aacttgtctt ggacaattag tgcagagaat
     1561 gacaaaagat aatattaatt atgctattgc tgttccaaga ataaaacagt atgaaagatt
     1621 aatagaagaa acgagcattc ttgctgttag taaattgaat ttacactgga tatttgttgg
     1681 gcctgatggc agtgtgacaa ttaaggaacc tgatagctct acgatgaaaa atagtaatgg
     1741 taatggaaga gaagtttctc ttgtgaatgg ggggtttaaa agatttatta cgggggattt
     1801 tatgccaact tgctcatatg ggaaagctcc tgttgtattt aaatcatctc ggcgagaaat
     1861 gtgggattgg ttggacgatt ttgttatttc aaatggggtg atcccaagta ttgctgatat
     1921 taaaaaagag gctgaatcgc ggagtgggaa atggacgctt tctaatgtta aaatagagta
     1981 tcagttttgg aaaatatatc acttgtataa ggggcatttt ttctatcaat ctgagtagat
     2041 tactcatttg ttcatcaaat gccatgatct tcttgagatt cattcgctca aagcgtaatc
     2101 tctttctcgg aaaacgaaaa aaccacctgg ggaggtggtt tttcgaaggt tcagtaagtt
     2161 ggggaacttc tgaaccgtgg taacagtctt gtgcgagaca tgtcaccaaa tctgtccttt
     2221 cagtgtagcc gcagtcaggc caccacttca agaactctcg atacatctct cgcacatcct
     2281 gtctaccagt ggccgttgtc aatggcgatt agtcgtgtct taccgggttg gactcaagac
     2341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2401 gcttggagcg aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg
     2461 ccacgcttcc cgaagggaga aaggcggaca ggtatctggt aagcggcagg gtcggaacag
     2521 gagagcgcac gagggagcca tcagggggaa acgcctgata tctttatagt cctgtcgggt
     2581 ttcgccacca ctgatttgag cgtcagtttc tgtgatgctt gtcagggggg gcggagccta
     2641 tggaaaaacg gccgctgtgc ggcctctcct tctccccatc acgcgatcgc tcggttttct
     2701 cgagctttta tacgcatgct cctgccgctc gccgcagccg aacggcagga gcgtagcgac
     2761 tgagtgagcg aggaagcgga aaagagaaca tccctacatt gagcactgac gctctgaggc
     2821 ctcttatgac gaaacaacag aaaacagcgc tgaacatggc taaattcatc cagaatcagt
     2881 ctctgctccc gcgccgaaca tcaccgttaa aaatcctgtg aacggacacg ctcacctgct
     2941 ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc tcggttaagg cgctgaaata
     3001 tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt gcggatgtga actacagcgg
     3061 cctgatttgc aaaaatccgt tccacctgga atggctggtg atggagtggc gcgaggaagc
     3121 ctataccctc gatgaactgg ctgattatct cgatttgagc gcctcagagc gtcgtagcat
     3181 cgataaacat tacgggatgg ggcgaaactg ccacctgttc gaaatgacgc gcaaatgggc
     3241 ttacagggcg attcgtcagg gctggccagc attctcacag tggcttgatg ccgtgattca
     3301 gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt tcacctcctg aatgtcgggc
     3361 tattggcaag agtattgcga aatacacgca caggaacttc acgccggaaa ctttcgcaca
     3421 gtatgtggct gatacgcaca cgccagaaat acaggccaag agaggcagga aaggtggcat
     3481 cgctaaaggc gaagcctacg atgacaagcg tttcatggcg ctatgtatgc tggagaatgg
     3541 atattctcag aaagctattg cggcgatgtt ggaggtttct actcgaacca ttcgaaactg
     3601 gaaaagcgga aaatagccta tatcagataa cagcgccttt ctggcgtttt tttgagcagt
     3661 aggtcttttg ccgtttttcc ccgttcagcc ctgcaatggt gctgttttcc gcatgattgt
     3721 gctggtagtg ctcgccgcag tctcatgacc gagcgtagcg agcgaatgag cgaggaagca
     3781 ccagggaacc ggaacacgga tggaggcact tacgcgtctg ggtttgggga tgtctccaga
     3841 tcgtacttaa tttccttaag tgcctgtaat atcctgtctt ttttatagca tagctcgcct
     3901 gtttttgtgt ctaccagtcc tgcttcgatc agtagcccag ccgcgagaaa gagtcggtga
     3961 ttgcgttccc gtcgttgttt ttttccctcg attcgtttct ggatcgtctg ttttcgctcg
     4021 ttctcctgtg cctgtaagtc gtttttttcc gctgttgcca gcacgtggag aagtttgcgt
     4081 tcctgttctg tgagtccggc ttcgttgtcg agcaggtcaa taatctgtct ctgtcgcgcg
     4141 gtctgttctc cgagcttcct gatgaatccg agccgtttct tcagccattc gatgttgctg
     4201 atgcgcatgt tcgtggtgct ccctggtgtt tttttgcggt gtgttgtggt cgagcagtat
     4261 atcgcttgcg gtcggcgggt gcagggggta tcttccgtgc taattcgtaa aattagaagg
     4321 aagcgcactt atatgtttct ccctgcgggc agaaacacgt gcgtaaaacc aagggcaaaa
     4381 acatcatggc aacctatcat ctcagcgtga aatttggcgg caagggtaaa gcccttgctc
     4441 atgccaatta catcacgcgg gaagataagt tcagtcagcg aaaagatctc gaacacaccg
     4501 agcacggcaa tatgccggaa tgggcgaaag atgaccccgc ccatttctgg caggctgcgg
     4561 atgcgttcga acgtgccaac ggttcaacgt accgggaaat cgagatagca ctaccccgtg
     4621 aactgaacga agcgcagcgg ctggagcttg tcagggattt tgtgaagcag gaaacgggcg
     4681 gtcaacatac gtattcgttt gcgattcaca atccgcaagc cagcattgac ggcggcgaac
     4741 agccgcacgc ccatatcatg atgagccagc gtgttgatga cggtattaag cgcagcccag
     4801 agcagtattt caggcgctat aacgccaaat ttccggcgcg tggcggcgcg cgcaaggact
     4861 cagggcataa tttaacccct acagaacaaa aacaggcact gaaagccctg cgggagcgct
     4921 gggagcgtac gcacaacgcg catatgaaac gatatggctt tgagcagggt tttattgata
     4981 gccggtcact gaaagcacag ggtatagacc ggaaaccgga acagcatctc ggttttgaag
     5041 cagccggacg actggacgac agccagaggc aggatattaa agacagccgg acgttttcag
     5101 tcgtggatgt gcctgaacag gcgaagcata tcgaccacag cgaccgtagc tggatgaatg
     5161 ccagcggtat cgaggtaaac cggaaggaac tggtcagatc tctgacgcat gatatctcgc
     5221 agcagtacgg gcgggagatg gctgcacggg tcacaaaaca gggaaatgtt gaaatagagc
     5281 gcgtgaaatc cctgtcacg
//
