LOCUS       NZ_CP056731             5523 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00316 plasmid pRHBSTW-00316_5,
            complete sequence.
ACCESSION   NZ_CP056731
VERSION     NZ_CP056731.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148572
            Assembly: GCF_013742375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00316
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.967439230937x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013742375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:50:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,917
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,694
            CDSs (with protein)               :: 4,694
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 36 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 24 of 107
            Pseudo Genes (multiple problems)  :: 17 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056731.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00316"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00316_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5106..5523,1..641)
                     /locus_tag="HV155_RS24300"
                     /old_locus_tag="HV155_24310"
     CDS             join(5106..5523,1..641)
                     /locus_tag="HV155_RS24300"
                     /old_locus_tag="HV155_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_071949222.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVIDLCPQAN
                     SSSMLLGGMVNGENTLNQIHSATQRKTISGYIEDRIRSPYIPTNTGSNYVINVSTYNSR
                     IPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWDNDDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGN
                     STPNNKKSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKNVSGRTIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(670..927)
                     /locus_tag="HV155_RS24305"
                     /old_locus_tag="HV155_24315"
     CDS             complement(670..927)
                     /locus_tag="HV155_RS24305"
                     /old_locus_tag="HV155_24315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057060549.1"
                     /translation="MGFNNDQNKDLQDKQADLLDQTYTLGWMDGYSEGYDDAIDDSQNA
                     LNKLENENELLNEIIEKKDNIINEMASGVGKALQTVKKNK"
                     /name="hypothetical protein"
     gene            complement(917..1102)
                     /locus_tag="HV155_RS24310"
                     /old_locus_tag="HV155_24320"
     CDS             complement(917..1102)
                     /locus_tag="HV155_RS24310"
                     /old_locus_tag="HV155_24320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109553065.1"
                     /translation="MTEDNVKNISDYRKKNDAVDANGSAVSSKNAPARLSEKLRNAIPS
                     GLRTQMRQQMRNKHGL"
                     /name="hypothetical protein"
     gene            1501..2052
                     /locus_tag="HV155_RS24315"
                     /old_locus_tag="HV155_24325"
     CDS             1501..2052
                     /locus_tag="HV155_RS24315"
                     /old_locus_tag="HV155_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058999046.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDAEYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKNPDYDDRNVITFDEYAEIHSPDAWKMLTGGQRY
                     PVAYLNAQEAFLRIGVTPEQLEWKKFHPSSPSLPNAVVPSLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(2256..2471)
                     /locus_tag="HV155_RS24320"
                     /old_locus_tag="HV155_24330"
     CDS             complement(2256..2471)
                     /locus_tag="HV155_RS24320"
                     /old_locus_tag="HV155_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2475..2960)
                     /locus_tag="HV155_RS24325"
                     /old_locus_tag="HV155_24335"
     CDS             complement(2475..2960)
                     /locus_tag="HV155_RS24325"
                     /old_locus_tag="HV155_24335"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_057060552.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAILSERNSGVQLSTCGEQGRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2995..3699)
                     /locus_tag="HV155_RS24790"
                     /old_locus_tag="HV155_24340"
                     /pseudo=""
     CDS             complement(<2995..3699)
                     /locus_tag="HV155_RS24790"
                     /old_locus_tag="HV155_24340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3638..3961)
                     /locus_tag="HV155_RS24335"
                     /old_locus_tag="HV155_24345"
     CDS             complement(3638..3961)
                     /locus_tag="HV155_RS24335"
                     /old_locus_tag="HV155_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_172971213.1"
                     /translation="MLTMWVTDDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLDKGRD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4610..4741
                     /locus_tag="HV155_RS24795"
     CDS             4610..4741
                     /locus_tag="HV155_RS24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059686908.1"
                     /translation="MHEPPVQSDCCACSGNYHLESNPERHDKTPLAAATGKGCTRYY"
                     /name="hypothetical protein"
     Typing          complement(4076..4248)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.678"
                     /coverage="100.0"
                     /evalue="2.02e-67"
     Typing          4173..4778
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.716"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2153..3648)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.984"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcacctttg gattaacgat ctaattagtg acatttgtaa ctcatgggat aatgacgaca
       61 gctgtgtgtt catcgactgc aaccctagct tctctattta tactgagcta gcgttaaccg
      121 cagcagaacg cctcttaatt ccattctctg ctgatggttc atcaaaaaga gcagttaaag
      181 ctgttctttc tttagtttat ggcgagcgcc gtcatcagaa cgaaccacag tcagatttct
      241 atttaaacag caatcgctac tctatgtcat taccgcaaat atacatgtat gtaggtaata
      301 gattaaccca aatgaacaac tcatctgcta gcgcatttaa gactgtggta acagagattg
      361 gcgatgagat ttggaaagtg tggcaaacaa aaccaagtct gttctgcatc catccaaatg
      421 gaaactcaac gccgaataat aaaaaatcat ttaggaacat gttccagtat gaagtgaatg
      481 atgcaaatac agcatctgtc gtatcaggag cacttgggat tccaattgcc acgctaaccg
      541 caggaaataa aaacgtttca ggaagaacaa taaaggtaaa tcaaacacag cttgataagc
      601 aagttcccaa cattgtagct cttgccggta tgattgagta aaaacaggcc cttgaaaggg
      661 cctgtttttt tatttgtttt tctttactgt ttgtaacgcc ttgcctacac cggacgccat
      721 ttcattgatt atattatctt ttttttcaat gatttcattg agtaattcgt tttcattttc
      781 cagtttattc aaagcattct gactatcgtc aatggcatca tcgtatcctt cagaataacc
      841 atccatccag ccaagagtat aagtctggtc cagaaggtca gcttgcttat cttgtaggtc
      901 tttattctga tcgttattaa agcccatgtt tgttcctcat ttgttggcgc atttgagttc
      961 tcagtccaga aggaattgca tttctaagtt tctcactcag cctggctgga gcatttttac
     1021 ttgaaacagc gctaccatta gcatcaacag catcattctt ttttctgtag tcactaatat
     1081 ttttaacgtt atcttcagtc atagaacact actcctatcg ctgtgaataa cccaatatac
     1141 actggagaat tgaacataac atcccttgcc cgcccccccg caaaggccct cagcgcgatc
     1201 ttagcgcgtc cgggccaaaa ggcagccctt tctctgcccg aaaccgcgaa aatccgtcct
     1261 gattagtttc cccggactgc atagccatgc agccgggtga atggctcagg ctttcaatgt
     1321 agatcttcac ctaaagccct attttacctg attcatcaaa aactcggagc agagagtctt
     1381 accactaacg gtaaaacagg caatgctcga gattttatcg cataaaatta ttgatacctt
     1441 aaattctatc gtatagatta agtaaaaaat gagattcatt cacctagtaa ggatttctat
     1501 atgaaagata tgaaggctgt tgttgttttt accggaaaag atttgaacat aatgagaact
     1561 gagggggggt caggctactg gcatgccaga acagacaggc ttaacgatgc cgaatacctc
     1621 atagcagtaa gaaacagaag ggaaacgtgg gcagtaaaag acctggaaca cggcacagcc
     1681 ttcctgattg caaaaattac cgggtgcttt aaaaatcccg attacgatga caggaacgtc
     1741 ataacctttg atgagtatgc agaaattcac tctccagacg catggaaaat gctaaccggt
     1801 ggtcagcgct atcctgttgc ttacctgaac gcacaggaag cctttttacg tattggggta
     1861 acaccggaac aactggaatg gaagaaattc catccttcat ccccatcgct tcctaatgca
     1921 gtggttccta gtctggctga agagaaaaca gaaaaactct cccttaacga ggcaattgaa
     1981 agagccaaaa aggatattag caacgcaaca ggaattgata gctcagccat aacaataagc
     2041 atcaagatct gatgcagcgt gaacaacagg atagcagcct cacgttctaa catgcgagaa
     2101 ctgaatctgt gcgccagaag taagctcatg cttttccggc gcacagattc aaagtgacgg
     2161 accgttgtac gtccgctgtc gctgcgatcg ttccagctcc agcgctttct ggtgctgcgc
     2221 cgcgcgcgcc tgctcccggt gtgcctcgac acagttcagc cttgcgacaa ccggcgcagc
     2281 tgctcgctca aactctgcac ctgcctgctc aagcccgtga cgcgctcgct cagcgccgcg
     2341 ttctcccgtt gcataagacc agacatcgtc cgccattccg cgaaggcgtt ctcccactcg
     2401 tccagccttt tcgagtagtc ctgctgtagc tgctctaatg cgctcagcaa ctgtttttcc
     2461 agctctgtca tgtgctattt ccccgccagt atcatccagc tcgaatcctc tccgaaccgt
     2521 cccgcttccg ggttcaccct cacgcagcgg cgtccctgct ctccgcaggt cgagagctgc
     2581 acgccgctgt tcctctcgga cagtatggcc tgcgtactct tctgctcccg gatggtcgtg
     2641 taattgtcga gtatctgctg cccctgccac cacagaatgc ccgcgctcga ggcgatcagc
     2701 aggacggaga ccaggacgat ggtcagccac gtctggctga ccatgcgcag catccctttc
     2761 gtgcgctggc tcatggctga ggacagcttc cggtcgtggt cgaggatggc ggcgctgatt
     2821 ctcttctcgc tttcgctcag ttccgctctg acagacttct cgtgctcgct gaaggcggct
     2881 ttcagcatct cgccggtact ctgctgctgc gctttcgatt tctgctctaa gtccttcgcc
     2941 agcgttaaaa gactgttcat agatggctcc cttcagtcgg atgtttcgcc ccccgtccgg
     3001 gtcggcaatg ctgatgctgc tttttgtggt gcggatcacc tcaaaacctg cgctttccag
     3061 cgcatcagtg acgtcctgac gcgttttaag ctccccggac gaggctaagg ccagtaaacc
     3121 ccgcgtaatc gcctgagctg cttcctgctt cgcttccggc agcgcggacg gcgtgaccag
     3181 cgcccgccgg ttttccggcg cgttcgggtc gtgcagtccc agcctgccgt taacgatggt
     3241 ctgccaggca tcgatgcgcg gacggtcggc gcggtcgtaa tacggctgga gacgcctgcc
     3301 ggtcagcagc tccgtatttg ggatcaggaa gttcagctcc agccgcccct tgtcctggtg
     3361 ctcaacccac agcacgctgt actggtcttt atcaagtccg ggcatcagaa cccgctcgaa
     3421 gctcgccatc agcttttcac gctggccggg cggtaaatcc ttttcggcaa aggacaggac
     3481 gccggaggtg tattttttgg cgtagggcga ggcgtcgata agctcccgga cttcctccgg
     3541 ttttccctga agcacactgg cgccctcgcg ctgccggtct tttcccagaa ggtaatccac
     3601 cggcccggaa ccacctccgc gtccccgggg atgaaattta acgatcatca tcccgcccct
     3661 tatccagcac agcatgacgc aggcgctcca gtccggcatc gatggccatc agcgccgcca
     3721 caatctgcac cctgtcatgg ccggtaccgc ccccggcatt aacccggcgg gctatctggt
     3781 tcaggttatt acccatgccg gcgagctgcc gcagcagcgc cggcgagatc gagggaagtt
     3841 tgccggcgcg ggcgggcttc tcgtccaggc acgtctgccg catccaggcc gcaagctgtt
     3901 taccgtcgca gcgttccagc agacggcggt gctcatcgtc ggtcacccac atcgtgagca
     3961 ttttgctgcg tttgtctgcc aatccggcct cctgataacg ccgcgacggg cgggaaatct
     4021 gttcccggtc ggcgcggtgt tggacaagcc gcaggcgcgt cagtggtttt agcgggtttc
     4081 ggggcgcagc cctgaaccag tcacgcagcg ctagcggagt gtatactggc ttaatatgtt
     4141 ggcacaggtg cggaagtcag tgaagtgctt catgtggcag gagagaaatg cagcagcgca
     4201 gcgtaagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctgcg
     4261 ctcggtcgtt cggctgcggc gagcggtacc ggcttacagg cggggcggaa atttcctggg
     4321 agatgccagg aagaaactta acggggaagc gataaggcca cggcggagcc gtttttccat
     4381 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     4441 ccgacaggac tataaagata ccaggcgttt ccccctgatg gctccctcgt gcgctctcct
     4501 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt ctctcattcc
     4561 acgccagaca ctcagttccg ggaaggcagt tcgctccaag ctggactgta tgcacgaacc
     4621 ccccgttcag tccgactgct gcgcctgttc cggtaactat catcttgagt ccaatccgga
     4681 aagacacgac aaaacgccac tggcagcagc cactggtaag ggatgtacaa gatattacta
     4741 acgcagagtt cttgaagttg aagcctaact acagctaaac tgaaaggaca gatttggtgg
     4801 cagcggtctt gtacaccctg ttaccacggt tcagaagttc cccaacttac tgatccttcg
     4861 aaaaaccacc tccccaggtg gttttttcgt ttacggagca aaagattaca tcccacgact
     4921 aaggatctca agagatcctt tcaatcacgt tcctactatg ccaaagaagt catgccaata
     4981 cgcatcccct aaccagggtc tactgctcga aacacactca gttagctgaa acaaactaac
     5041 attagaacaa taatctctag tactctgacg ttaagctaac aacaaaacta gattaggagt
     5101 aatagatggc gatctatgcc gtatggaata acaaaggtgg tgtaggaaaa agttatctta
     5161 cctttcaact agcctctgag tacgccagac agaacccgac aaaaaaggtt ctggtgattg
     5221 atctatgccc gcaagctaac tcatcctcaa tgttacttgg cgggatggtt aatggcgaaa
     5281 atacgctaaa tcaaattcat tctgcgacac aaagaaagac tatttctgga tatattgagg
     5341 ataggatcag aagcccatac attccgacaa atacaggttc taactacgta atcaacgtat
     5401 ctacatacaa tagcagaata ccaaataatc tatatcttgt tactggcgat gaacaattag
     5461 aacttcaagc atcaagagtt gttggagcat cattcccagg gcctcacgac gcatggcgaa
     5521 cag
//
