LOCUS       NZ_CP056726             2494 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00333 plasmid pRHBSTW-00333_8,
            complete sequence.
ACCESSION   NZ_CP056726
VERSION     NZ_CP056726.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148580
            Assembly: GCF_013738375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00333
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73.7468655459429x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013738375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:54:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,069
            Genes (coding)                    :: 4,955
            CDSs (with protein)               :: 4,955
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 28 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 31 of 114
            Pseudo Genes (multiple problems)  :: 17 of 114
            Pseudo Genes (short protein)      :: 1 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056726.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00333"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00333_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2458..2494,1..191)
                     /locus_tag="HV163_RS25760"
                     /old_locus_tag="HV163_25760"
     CDS             join(2458..2494,1..191)
                     /locus_tag="HV163_RS25760"
                     /old_locus_tag="HV163_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            557..742
                     /locus_tag="HV163_RS25765"
                     /old_locus_tag="HV163_25765"
     CDS             557..742
                     /locus_tag="HV163_RS25765"
                     /old_locus_tag="HV163_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1033..1251)
                     /locus_tag="HV163_RS25770"
                     /old_locus_tag="HV163_25770"
     CDS             complement(1033..1251)
                     /locus_tag="HV163_RS25770"
                     /old_locus_tag="HV163_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(790..877)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1515..2043
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ttgcgctttc agccctcacc ggctgcacgc gcacaagcta tgccatacac accaatgacg
       61 gtcgcactat tgtcagcgac ggcaaaccta aagaatcaga ctcaggcctg cttggctata
      121 cagacgcgaa tggtgtcaaa cagcagatca ataaaaccga cgttaaagaa gtctccgaaa
      181 ttccgcactg agcatcttct ccgaaaaagg ccgctaatgt ggcctttttc tttacctgtg
      241 gttctccgcc aaaatcccag caaattgcat caccaaagct aaaagctttc agggttgtct
      301 aaaaagcaca agacataaga ggaagtgcgg tattttataa tcaagccccc aagaatttac
      361 cataacatcc gttgcccgca ccgcctgaga cgcgttcagc gcgttcctga cgaaaccatg
      421 acaaaaacca caacaaacca ccccggaacc cgtcagaaac gcgcctgtta aattttaacg
      481 gcatgcatga ctatgcacca gaatgacgcc atgctctttt cacgcaaaaa tcatcaccag
      541 acggggaaaa tcaccagtga ccagacagga atccgccgcc ctcaatatgg ccaaatttat
      601 ccgcgcacag acacttctcc tccttgagcg gctcgagcag atggatctgg atgaggctgc
      661 cggctgctgt gagcacctgc acgatcaggc cgaagcgctt tacgccatgc tgaacgcaca
      721 gataggcgag gaaaatgcgt gaaaatcggt gaacgggtgc gcaattcagt gcgcggccgt
      781 gaggcgatgg cggggtgtcg gggcgcagcc ctgaccaggg tatttgtgat gccggcgcgt
      841 gcgcggtatt acaaatgcac atcctgtccc ggaacggaca ccgggaaaca gcaaaaaaaa
      901 ccgggcggca cgcccggaac tcaatcaagt tagattagat tactctcact cgtccataac
      961 agcatcatgg aacgacgacc accgtccgtg acggccgcct cgtttaagta tggacagaaa
     1021 tacagaaaat gctcaggacg aaatgtaatg aatgcgaacg gattcaagaa attcgagcat
     1081 gacagtcctt acggccggtt cggtttcaga caaaatctgc cggtatgcat ccagcatcat
     1141 ggctccggca tcccctccgg cacgccgtag ccagaccgaa acaacggaca caagcaggtg
     1201 tcgctcatca tcactaagag tcatcagggc tccggaagaa aaaccaaaca tggcttacct
     1261 actgttaacg gcaacgcaga agaaaggttt acagggtagc gggcagggat gaaaaaaaaa
     1321 aattgcagtc aggccggaac ggctgctgaa ccggtacagg cgggttccgg gcgcagcgcc
     1381 ggaccagtca cggagcacta gcggagtgta tactggctta agatgtagca tggcagtaac
     1441 acatagcatt aatgccacat aagacaggcg cagaaagtga aagaaaacgc gtcaggagaa
     1501 aatgtgatac agaatatatt ccgcttcctc gctcactgac tcgctccgct cggtcgttcg
     1561 gctgcggcga gcgggacggg cttacggatg gatcagaaat ttcctggggg atgccaggaa
     1621 gatacttaac gaagaaagca taaggccgcg ccaaagccgt ttttccatag gctccgcccc
     1681 cctgacaagc atcacagaat ctgacgctca aatcagtggt ggcgaaaccc gatgcggctt
     1741 aaagatacca ggcgtttccc cctggtggct ccctcgtgct cccccgcatt cctgcctttc
     1801 ggtttaccgg tgtcactccg ctgttatggc cgcggtttct cattccacgc ctgacactca
     1861 gttccgggaa ggcagttcgc tccaggatgg actgtatgca cgaacccccc gttcagtccg
     1921 accgctgcgc cttatccggt aactatcaac ttgagtccaa cccggaaaga cacgactaat
     1981 cgccattggc aacggccatt ggcaaacagg atgtgcgaga gatgtctcaa gagttcttga
     2041 agtggcggcc ttagtgaggc tacactgaaa ggacaaattt ggtgacatgt ctcgcacaag
     2101 actgttacca cggttagggg atctgccagg attacctaac cttcgaaaaa ccacctgcca
     2161 gggtggtttt ttcgtttaca gggcaagaga ttacgcgcgg aaaaaaagga tctcaagaag
     2221 atcctttata aattagacat ccaacctcac aaagaggaat acttaaatca tcttggtata
     2281 tgtatcggcc ggattacatc tcatataagc ctcctccccg cctgacacca cactttgacc
     2341 atagcacctc gcgcctaact cgatatacag cccaccatca agcctctttt cacataaaaa
     2401 atgacctata gttagatggt tagtgaccaa aactgatatg gataaaagga gctggctttg
     2461 aaaaagattt ttgtttcagt gttcgcagcg gcag
//
