LOCUS       NZ_CP056725             4783 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00333 plasmid pRHBSTW-00333_7,
            complete sequence.
ACCESSION   NZ_CP056725
VERSION     NZ_CP056725.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148580
            Assembly: GCF_013738375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00333
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73.7468655459429x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013738375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:54:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,069
            Genes (coding)                    :: 4,955
            CDSs (with protein)               :: 4,955
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 28 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 31 of 114
            Pseudo Genes (multiple problems)  :: 17 of 114
            Pseudo Genes (short protein)      :: 1 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056725.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00333"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00333_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            50..373
                     /locus_tag="HV163_RS25725"
                     /old_locus_tag="HV163_25725"
     CDS             50..373
                     /locus_tag="HV163_RS25725"
                     /old_locus_tag="HV163_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            582..>1016
                     /locus_tag="HV163_RS26065"
                     /old_locus_tag="HV163_25730"
                     /pseudo=""
     CDS             582..>1016
                     /locus_tag="HV163_RS26065"
                     /old_locus_tag="HV163_25730"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1051..1542
                     /locus_tag="HV163_RS25735"
                     /old_locus_tag="HV163_25735"
     CDS             1051..1542
                     /locus_tag="HV163_RS25735"
                     /old_locus_tag="HV163_25735"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1549..1764
                     /locus_tag="HV163_RS25740"
                     /old_locus_tag="HV163_25740"
     CDS             1549..1764
                     /locus_tag="HV163_RS25740"
                     /old_locus_tag="HV163_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2435..2677)
                     /locus_tag="HV163_RS25745"
                     /old_locus_tag="HV163_25745"
     CDS             complement(2435..2677)
                     /locus_tag="HV163_RS25745"
                     /old_locus_tag="HV163_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            2864..3181
                     /locus_tag="HV163_RS25750"
                     /old_locus_tag="HV163_25750"
     CDS             2864..3181
                     /locus_tag="HV163_RS25750"
                     /old_locus_tag="HV163_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3384..3740
                     /locus_tag="HV163_RS25755"
                     /old_locus_tag="HV163_25755"
     CDS             3384..3740
                     /locus_tag="HV163_RS25755"
                     /old_locus_tag="HV163_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(<4060..4182)
                     /locus_tag="HV163_RS26195"
                     /pseudo=""
     CDS             complement(<4060..4182)
                     /locus_tag="HV163_RS26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          4545..4718
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(4070..4578)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          51..367
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.5700000000000008e-59"
     Typing          364..1856
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cccgtcccgg cgttatcagg aggccggttt gtcagagaaa cggaacaaga tgctcaccat
       61 gtgggtgacg gagggcgagc accggcggct gcttgagcgc tgcgacggca ggccgctggc
      121 ggcgtggatg cggcagacct gcctggacga aaagcctgcg cgcaccggaa agctcccctc
      181 actctcgccg gcgctgctgc gccagcttgc cggtatgggc aataacctga accagatagc
      241 ccgccgtgtt aacgccggcg gcggcaccgg ccatgacagg gtacagattg tggcggcgct
      301 gatggccatc gatgccgggc ttgagcggct gcggcacgcc gttcttgaga aaggaacgga
      361 cgatgatcgt taaatttcat ccacgcggtc gcggcggtgg cgccgggccg gtggactatc
      421 tgctgggtaa agaccggcag cgtgaaggcg ccagcgtcct gcaggggaag ccggaagaag
      481 tccgggagct gattgacgcg tcgccttacg tgaagaaata cacctccggc gtactgtcgt
      541 ttgcggaggc cgatctgcct cccggccagc gggaaaaact gatggccagc ttcgagcggg
      601 ttctgatgcc cggactcgat aaagatcagt acagcattct gtgggtggag cacgcggaca
      661 aggggcgtct ggagctgaac tttctgatcc ccaataccga actgctgacc ggcaggcggc
      721 tccagccgta ctacgaccgc gccgaccggc cgcgcatcga tgcctggcag accatcgtga
      781 acggcagact ggggctgcat gacccgaacg cgccggaaaa ccgccgggcg ctggtcacgc
      841 cgtccggact gccgaaaacg aagcaggagg ccactgaagc catcacgcgc ggactgctga
      901 cccttgcttc gtccggggag ctgaaaaacc gtcaggacgt cactgaggcg ctggaacggt
      961 caggttttga ggtggtgcgc accacgaaaa gcagtatcag cattgccgac ccggacgggg
     1021 ggcgaaacat ccgactgaag ggagccatct atgagcagtc ttttaacgct ggcgaaggac
     1081 ttagagcagc aatcgaaagc gcagcagcag agtaccggcg agatgctgaa agccgcattc
     1141 agcgagcacg agcagtctgt cagagcggaa ctgagcgcaa gcgcgaggag aatcagcgac
     1201 gccatcctcg cacacgagca gagtatgagc gaggcgatgg agaaaaaccg gcggagcgta
     1261 ctgcggactg ccgggcggac atggttcacc atcctgatgg tgtcagcgct gctgatcggc
     1321 acgagcggga gcatactgtg gtggcagggg cagcagataa ccgacaatta cacgcatctt
     1381 cgtcagcagg aagataccct ggcgaaaatg actgcccgga cgtggggagt gcgctatcag
     1441 gagagcagcg acggccggag gtttcttatc ctgccgcccg ggatgcaaac cgaagccatc
     1501 ccctacgacg ggacgacatg gatccgcctg aaacaggagt agcggaaaat gacagagctg
     1561 gaaaagcagt tgctgagcgc attagagcag ctacagcagg actactcgca aaggctggac
     1621 gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg ggcttatgca acgggagaac
     1681 gcggtgctga acgagcgcgt gtcggacttg agcacgcagg tgctgagttt aagcgagcag
     1741 ctgcgccgct tgtccgggaa ctgaatgcca tcgagctccg gcagagggag aatgagtatc
     1801 agaaaacact ggagctggag cgccagccgc agaaaaccta ccgcgggccg acactttaaa
     1861 ccatccaggc agctgacaac ccgcacgaag aggggctctt atttctcccg ctaaataggt
     1921 tcgtgatcaa aaaggtcacg acttattcag gagcgggaaa tgaagctgtt aaatctcatt
     1981 gaatcattgc ttaactgcct gtatctggcg gcggagctgt gggactggct ggagaagcat
     2041 ttgtcggttt ttaaggcatt ggcgcggatt ttagtgtgac tttaacatcc atgcatctgg
     2101 ctgcatgact atgcaccagt gtaatattca gcggctgcgt ttctggtgtt ttttcgttat
     2161 gtctgtcgcc actttctcgc gtctggcacc ggaaacgcag ccaaccccgt ttcagcggtg
     2221 cgcccaacgg atgttatggt aaatcagtat cagctgcaga tataaaaaag ccgccaggct
     2281 ggcggcttgc tgttattttc gtggctttcc tgcatctgag cgttttcctc tgacccttcc
     2341 gtcattgtta cggtcacgag gttgagttcc gccgcctggt ttaggaacga caacgtgtgg
     2401 gttgagtggt cgacgatctg ccattttcta tacctcactg agcataaaca aacaattgcc
     2461 aacgaatgtt cccttgcgcg ggggtatgtt ggtggtggtt ttgtggtggg attgttttcc
     2521 cgcctgcgat agttacttct ttaccgcaac cggtacattt ataaatccct tcatattcac
     2581 atgtttctcc cggcttatat tcagtgtcaa aaaccgagtt aaacttcgta tctgcttcaa
     2641 aaatcttgct tttgtttcct gagcgaacaa gagccatgca acctccaaat tatcatttat
     2701 tttgcacacc cgcactgtgc atgagtgcat tctacagcat agccattgat tgcacaagtg
     2761 cactgtgctg ttgtgcattt gatatgtttg ctgtactata tcggaggtaa gaaggtgatc
     2821 gaaaaaattg ccaagttgcg gcaaaaattg aggtaaaaga aaaatgcagc aagatcatga
     2881 acatgatgat tgccgcattc agtttcgaga cgattggcta taccagtttt acaaatatgg
     2941 taaagccaat cgaaagattc caactgcgat tagtgcttca ttaggtcgca agctggatat
     3001 gctgaatgct gctgataaag ttggagattt gcggtctccc ccgggaaacc gatttgagaa
     3061 tctcaaccct ccattgacag ggtattcatc catacgggtg aatgagaagt accgcttgat
     3121 atttaagtgg ggtgaaaacg gagtcgagga tttgtatctc gatccacatg attaccgata
     3181 gataacaggt cgtggctgtt atcttataaa acaagtaggt gaggcgttcc ctcacctaca
     3241 ttaaaatgtc attacaaata atgattaata aaaaaaatga cgtgactata tttaagataa
     3301 ttactcatcg ctacagctgg taatagacga aatacttatc ttcctcatct aacagagaga
     3361 agtaaaaaat ttaggagtaa agcatgtccc aagcaactag aaaaccatca actgtagggg
     3421 atattattct ttatgaatat ctcgaaccaa gtggtttaaa aatcaatgac ttggctgaaa
     3481 tgttgaatgt gcatagaaac tctgccagtg ctttagttaa caataataga aagctgtcta
     3541 ccgatatggc ttttaggttg gctaaagcct tcgataccac tgcaaagttc tggctaaata
     3601 tacagcagtc tgttgattta tgggaggtta ctagtagccc tcgattgcaa gaagaaatag
     3661 acagagtgat tcccgctcat gaaatcatag aaaggaaaca cattgttgat agttctgaga
     3721 agaaacatgc tattgcatga ttttttttaa aaaaatataa gttatccctc aaaaagaagg
     3781 cttctataac tagaagcctt ctttttgagg gataaaaacg ctcatgttaa ctttcttctt
     3841 acgatcttct tgagatccgt gctctgtacg cgtaatctct tgctctgtaa acgaaaaaac
     3901 cgcctgggga ggcggttttt cgaaggttaa gtcagtcggg gaactgctta acctggtaac
     3961 tggcttcagc agagcgcaga taccaaatac tgtccttcca gtgtagccgt agttaggcca
     4021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4081 ggctgctgcc agtggcgttt tgtcgtgtct ttccgggttg gactcaagtc gatagttacc
     4141 ggaacaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     4201 aactgcctac ccggaaccga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg
     4261 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccatcagggg
     4321 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     4381 ttctgtgatg ctcgtcaggg gggcggagcc tatggaaaac cggctctgcc gtggccttat
     4441 cgcttccctg ttaagtttct tcctggcatc ttccaggaaa tttccgcccc gttcgtaagc
     4501 ccgcaccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4561 atatatcctg tatcgcatat tctgctgacg cgccgttgcc gccttttttc tcctgccaca
     4621 tgaagcactt cactgatttc cgtatccgtg ccaacatatt aagctagtat acactccgct
     4681 agcgctacgt gactggttca gggctgcgcc ccgaaacccg ccaaaagcca ctgacgcgcc
     4741 tgcggcttgt ccaaccccgc gccgaccggg aaaggctttc ccg
//
