LOCUS       NZ_CP056724             4859 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00333 plasmid pRHBSTW-00333_6,
            complete sequence.
ACCESSION   NZ_CP056724
VERSION     NZ_CP056724.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148580
            Assembly: GCF_013738375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00333
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73.7468655459429x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013738375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:54:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,069
            Genes (coding)                    :: 4,955
            CDSs (with protein)               :: 4,955
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 28 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 31 of 114
            Pseudo Genes (multiple problems)  :: 17 of 114
            Pseudo Genes (short protein)      :: 1 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00333"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00333_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            89..412
                     /locus_tag="HV163_RS25690"
                     /old_locus_tag="HV163_25690"
     CDS             89..412
                     /locus_tag="HV163_RS25690"
                     /old_locus_tag="HV163_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            621..>1055
                     /locus_tag="HV163_RS26060"
                     /old_locus_tag="HV163_25695"
                     /pseudo=""
     CDS             621..>1055
                     /locus_tag="HV163_RS26060"
                     /old_locus_tag="HV163_25695"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1090..1581
                     /locus_tag="HV163_RS25700"
                     /old_locus_tag="HV163_25700"
     CDS             1090..1581
                     /locus_tag="HV163_RS25700"
                     /old_locus_tag="HV163_25700"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1588..1803
                     /locus_tag="HV163_RS25705"
                     /old_locus_tag="HV163_25705"
     CDS             1588..1803
                     /locus_tag="HV163_RS25705"
                     /old_locus_tag="HV163_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2275..2766
                     /locus_tag="HV163_RS25710"
                     /old_locus_tag="HV163_25710"
     CDS             2275..2766
                     /locus_tag="HV163_RS25710"
                     /old_locus_tag="HV163_25710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(2823..3251)
                     /locus_tag="HV163_RS25715"
                     /old_locus_tag="HV163_25715"
     CDS             complement(2823..3251)
                     /locus_tag="HV163_RS25715"
                     /old_locus_tag="HV163_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_058676822.1"
                     /translation="MGYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            3536..3964
                     /locus_tag="HV163_RS25720"
                     /old_locus_tag="HV163_25720"
     CDS             3536..3964
                     /locus_tag="HV163_RS25720"
                     /old_locus_tag="HV163_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          4662..4835
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(4192..4738)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.091"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          403..1895
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcttgtccaa ccccgcgccg accgggaaag gctttcccgc ccgtcccggc gttatcagga
       61 ggccggtttg tcagagaaac ggagcaagat gctcaccatg tgggtgacgg aggacgagca
      121 ccggcggctg cttgagcgct gtgacggcag gcagctggcg gcgtggatgc ggcagatctg
      181 cctggacgaa aagccggcac gttccggaaa gctcccctcg atctcgccgg cgctgctgcg
      241 ccagcttgcc ggcatgggca ataacctgaa ccagatagcc cgccgtgtta acgccggcgg
      301 cggcaccggc catgacaggg tacagattgt ggcggcgctg atggccatcg atgccggcct
      361 tgagcggctg cggcacgccg ttcttgagaa aggaacggac gatgatcgtt aaatttcatc
      421 cgcgcggccg cggcggtggc gccgggccgg tggactatct gctgggtaaa gaccggcagc
      481 gtgaaggcgc caccgtcctg caggggaagc cggaagaagt ccgggagctg attgacgcgt
      541 cgccttacgt taagaaatac acctccggcg ttctgtcgtt tgcggaggcc gatctgcctc
      601 ccggccagcg ggaaaaactg atggccagct tcgagcgggt tctgatgccc ggactcgata
      661 aagatcagta cagcattctg tgggtggagc acgcggacaa ggggcgtctg gagctgaact
      721 ttctgatccc caataccgag ctgctgaccg gcaggcggct ccagccttac tacgaccgcg
      781 ccgaccggcc gcgcatcgat gcctggcaga ctatcgtgaa cggcagactg gggctgcacg
      841 acccgaacgc gccggaaaac cgccgggcgc tggtcacgcc gtccgggctg ccgaaaacga
      901 agcaggaggc cgcagaagcc attacccggg gactgctgac ccttgcttcg tccggggagc
      961 tgaaaagccg tcaggatgtc actgacactc ttgaaggtgc aggttttgag gtggtgcgca
     1021 ccacgaaaag cagtatcagc attgccgacc cggacggggg gcgaaacatc cgactgaagg
     1081 gagccatcta tgagcagtct tttaacgctg gcgaaggact tagagcagca atcgaaagcg
     1141 cagcagcaga gtaccggcga gatgctgaaa gccgcattca gcgagcacga gcagtctgtc
     1201 agagcggaac tgagcgcaag cgcgaggaga atcagcgacg ccatcctcgc acacgagcag
     1261 agtatgagcg aggcgatgga gaaaaaccgg cggagcgtac tgcggactgc cgggcggaca
     1321 tggttcacca tcctgatggt gtcagcgctg ctgatcggca cgagcgggag catactgtgg
     1381 tggcaggggc agcagataac cgacaattac acgcatcttc gtcagcagga agataccctg
     1441 gcgaaaatga ctgcccggac gtggggagtg cgctatcagg agagcagcga cggccggagg
     1501 tttcttatcc tgccgcccgg gatgcaaacc gaagccatcc cctacgacgg gacgacatgg
     1561 atccgcctga aacaggagta gcggaaaatg acagagctgg aaaagcagtt gctgagcgca
     1621 ttagagcagc tacagcagga ctactcgcaa aggctggacg aatgggagag cgccttcgcg
     1681 gaatggcgga gcatgtgtgg gcttatgcaa cgggagaacg cggtgctgaa cgagcgcgtg
     1741 tcggacttga gcacgcaggt gctgagttta agcgagcagc tgcgccgctt gtccgggaac
     1801 tgaatgccat cgagctccgg cagagggaga atgagtatca gaaaacactg gagctggagc
     1861 gccagccgca gaaaacctac cgcgggccga cactttaaac catccaggca gctgacaacc
     1921 cgcacgaaga ggggctctta tttctcccgc taaataggtt cgtgatcaaa aaggtcacga
     1981 cttattcagg agcgggaaat gaagctgtta aatctcattg aatcattgct taactgcctg
     2041 tatctggcgg cggagctgtg ggactggctg gagaagcatt tgtcggtttt taaggcattg
     2101 gcgcggattt tagtgtgact ttaacatcca tgcatctggc tgcatgacta tgcaccagtg
     2161 taatattcag cggctgcgtt tctggtgttt tttcgttatg tctgtcgcca ctttctcgcg
     2221 tctggcaccg gaaacgcagc caaccccgtt tcagcggtgc gggcaacaga tgttatggta
     2281 aatcaaccgt ccgttgataa aggttctgat aatacccatg taggtgatgt tgtttgcttt
     2341 ttatctacac actgcctgat ggagctattt atgaatgctg atgaagttac acgaattctt
     2401 gcgcaatcga gtcaaaatga ttggattgtg gatgatgaga caggggcgtt tacttataaa
     2461 aatgatttag cactgcacat taagagagct gattacgacg actacaactc gtttaatgag
     2521 ccttgggctg ttcgtcatcc tgatagtaat gcgtgttcag tcgagtacac cgttcactat
     2581 ggttcttcgt ttgttgatag aaaaaccttg gtttccgtcg atggtcatcg tgcaaccttg
     2641 cctatgcctg catccgcaac ggatctaaac gtgtcatcgg ctgatgtgaa tttcgccaaa
     2701 attgtaaaca ttggcgatcg cgttgatgaa tacattcaac gttctggtct tacgattaag
     2761 ccctaattta acgataagcc cggcctccaa atgcgggctg ttcctgcatg taaccagcat
     2821 aactaatcgg cttttttcct tactgtatat attggctttt tatgtcgcgg ctttcttact
     2881 tgctcggaca cagaaggacc aattatgtct aaataatcta caagctttgt aaggtcgtat
     2941 atgttgcggc caccatatcg gttgtcgctt tttgatgtcg gttttttatc taagagattg
     3001 gctttttcta ggtcattaat agccctttgc accgtcctta ctgacacccc catccgtata
     3061 gccaggctct ccattgaggg gtgcggccac tcctgtagat tccaccagtg tactatgaga
     3121 ttcagcaagg tattgaatgc tacagggctc aatttcttct cattttgtat aaaaaacaac
     3181 acagtaggta ctgcagtcca tcctaatgac agacctccag ccttccattt tttttctatt
     3241 gcgtaaccca ttgattatcc tgtaaatatt ttttttgaga tatatataat ataagtattc
     3301 atatgaatag ggtgacacca gcgccattat tacacaacta taaaaggcta atctgagaac
     3361 agaaaaacat ttctatctgc attaaatacg gagttagtga tatgaaaacc aaacaacatc
     3421 atgatcttgt aaaagatact gaaatatttg gtgtatcctt gtctctgttg atttccagag
     3481 cgaatgagag atctgttaca atgccatctg gtctaagtag ggagcagaga agagcatggg
     3541 cagcggatca agcacgcaaa atccacaatt gaatgttgtc tctttctctg agacattcaa
     3601 tctttattac acatcccgct tttctcaaaa aacactcgat aaaatcgatg acttcatcga
     3661 acatttcgag aagtacaatc tttttggcga tgccttaaaa ggccatccgg cttggattgg
     3721 caaggtatcg ccatcttgga acgtgcctga cagttacgaa aacaaagaag ctattgaaga
     3781 acatgccagg gctaataaat tgtggcatgc ccatataggc gatcccgttt tcatcgatac
     3841 atttcacggg aagtacaaag tctctgattg ggtcatccac ttccaacgtc tagcgccgaa
     3901 ccatataaaa ctgttagagc ttgggtatca tgaccctatg caactccccc aaaatttcga
     3961 atgatctctt gttcggatga atgatccctt gagattcgtt gaccctcgtc gtaatctctt
     4021 gcggtgaaaa cgaaaaaacc acccggcaag gtggcttttt cgaaggttcg caagagttga
     4081 cgctcttttg aaccgcggta actggcttgg aggagcacag tcaccaaaac tgtcctttca
     4141 gtgtagcctt atcgcacccg aaacttcaag actctcctcc tctaaacctg ttaccagtgg
     4201 ctgctgccag tggcgctttg tcgtgttttt ccggattgga ctcaagttga tagttaccgg
     4261 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
     4321 ctgccttccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
     4381 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc accaggggga
     4441 aacgcctggt atctttaagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt
     4501 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg gccttatcgt
     4561 ttccctgtta agtttcttcc tggcatctac caggaaattt ccgccccatt cgtaagccca
     4621 caccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaata
     4681 tatcctgtat cgcatattct gctgacgcgc cggtgccgcc ttttttctcc tgccacatga
     4741 agcacttcac tgattcccgc acccgtgcca atataataag ccagtataca ctccgctagc
     4801 gctacgtgac tggttcaggg ctgcgccccg aaacccgcca aaagcctgac gcgcctgcg
//
