LOCUS       NZ_CP056723             6974 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00333 plasmid pRHBSTW-00333_5,
            complete sequence.
ACCESSION   NZ_CP056723
VERSION     NZ_CP056723.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148580
            Assembly: GCF_013738375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00333
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73.7468655459429x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013738375.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:54:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,069
            Genes (coding)                    :: 4,955
            CDSs (with protein)               :: 4,955
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 28 of 114
            Pseudo Genes (incomplete)         :: 73 of 114
            Pseudo Genes (internal stop)      :: 31 of 114
            Pseudo Genes (multiple problems)  :: 17 of 114
            Pseudo Genes (short protein)      :: 1 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056723.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00333"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00333_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6696..6974,1..872)
                     /locus_tag="HV163_RS25640"
                     /old_locus_tag="HV163_25640"
     CDS             join(6696..6954,6954..6974,1..872)
                     /locus_tag="HV163_RS25640"
                     /old_locus_tag="HV163_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612101.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_087855188.1"
                     /translation="MTKPASTSKKPRKQYTPEFRNEALKLAERIGVAAAARELSLYESQ
                     LYAWRSKINNARSSSEREQEMSVEIARLKRQLAEQAEELAIPPKGRDILREAPEMKYVF
                     IEKHRAEFSVKAICRVLRVARSGWYAWRLRRQQITPRQQFRLVCDTAVRQAFTEAKQRY
                     GAPRLADELPEYNVKTIAASLRRQGLRAKAARKFSPVRYREHGLPVSENLLKQDFYASG
                     PNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSSRMTAQLACDALQMALWRRKR
                     PENVIVHTDRGGQYCSADYLALLKRHNLHGSMSAKGCCYDNACAESFFHSLKVECIHGE
                     RLISREIMRMTVFNYIECDYNRWRRHSACGGLSPEQFENQNLA"
                     /name="IS3 family transposase"
     gene            <902..>1018
                     /locus_tag="HV163_RS25645"
                     /old_locus_tag="HV163_25645"
                     /pseudo=""
     CDS             <902..>1018
                     /locus_tag="HV163_RS25645"
                     /old_locus_tag="HV163_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(1029..1661)
                     /locus_tag="HV163_RS25650"
                     /old_locus_tag="HV163_25650"
     CDS             complement(1029..1661)
                     /locus_tag="HV163_RS25650"
                     /old_locus_tag="HV163_25650"
                     /inference="COORDINATES: protein motif:HMM:NF027277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4755 domain-containing protein"
                     /protein_id="WP_139137146.1"
                     /translation="MKIYYALKRIAYIAGALSIGCGSLPMIFSKTPALQYWVLGAFLIF
                     IGYCVSKLEFDKSKYIKYLNEKNDGIYIYYAKFGGEEHEIKINTKNKTIELKSGKNSAT
                     LNFDNIKSWRYNVSGISKVNVIGSTNSVMGNFTALSQASAINNQAGYSAWDNNGFFIHT
                     DALANPVWHIKIIPKDVAINLKSDKFWKSVAGECEVWMNVMNRAINK"
                     /name="DUF4755 domain-containing protein"
     gene            1910..2608
                     /locus_tag="HV163_RS25655"
                     /old_locus_tag="HV163_25655"
                     /pseudo=""
     CDS             1910..2608
                     /locus_tag="HV163_RS25655"
                     /old_locus_tag="HV163_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            3502..3693
                     /locus_tag="HV163_RS25660"
                     /old_locus_tag="HV163_25660"
     CDS             3502..3693
                     /locus_tag="HV163_RS25660"
                     /old_locus_tag="HV163_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4194..4355
                     /locus_tag="HV163_RS25665"
                     /old_locus_tag="HV163_25665"
     CDS             4194..4355
                     /locus_tag="HV163_RS25665"
                     /old_locus_tag="HV163_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_049122912.1"
                     /translation="MAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDAEHLFRTLSSR
                     LDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4355..4777
                     /locus_tag="HV163_RS25670"
                     /old_locus_tag="HV163_25670"
     CDS             4355..4777
                     /locus_tag="HV163_RS25670"
                     /old_locus_tag="HV163_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_049122914.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHGLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            5532..5849
                     /locus_tag="HV163_RS25675"
                     /old_locus_tag="HV163_25675"
     CDS             5532..5849
                     /locus_tag="HV163_RS25675"
                     /old_locus_tag="HV163_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225865423.1"
                     /translation="MVDLLAVDVCSYGEIVATDAQVKQINALIKKYPDSCSICHQEYDE
                     DYVTYTVFGYDKNQKMQVTTGCCARTLTELVLLGVCGCFDPDERDEIMENHPMAKKFFS
                     N"
                     /name="hypothetical protein"
     gene            complement(5912..6355)
                     /locus_tag="HV163_RS25680"
                     /old_locus_tag="HV163_25680"
     CDS             complement(5912..6355)
                     /locus_tag="HV163_RS25680"
                     /old_locus_tag="HV163_25680"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_064188129.1"
                     /translation="MIAVSLPDELLQKLDNAVAKTGKKRSYLIRESIQMYLNQIENTHE
                     KKEIILNTSKPFYEILIEEFQVEKELMTEARKTEFTMFSDNGKLYVVNSKGNTRKLEAV
                     YVNNFFEEYKKTGSMSPSSYQDITFNSSYLLAALKYLIEKELI"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            6512..>6634
                     /locus_tag="HV163_RS25685"
                     /old_locus_tag="HV163_25685"
                     /pseudo=""
     CDS             6512..>6634
                     /locus_tag="HV163_RS25685"
                     /old_locus_tag="HV163_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     Typing          complement(3002..3627)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.472"
                     /coverage="99.0"
                     /evalue="5.52e-168"
ORIGIN
        1 ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcgggct gagttcagcg
       61 tcaaagccat atgccgggta ctgcgggtcg cccgtagcgg ctggtatgcg tggcgtctgc
      121 gtcgtcagca gataacacca cgtcagcagt tccggctcgt ctgtgatact gctgtccgtc
      181 aggcattcac tgaggcaaaa cagcgttatg gtgcgcctcg tctcgctgac gaactgcctg
      241 agtacaacgt caaaaccatt gccgccagcc tgcggcgtca ggggcttcgc gcaaaagcgg
      301 cccggaagtt cagcccggtc cgataccgcg aacatggcct gccggtgtct gaaaatctgc
      361 tgaaacagga tttttacgcg agtggcccga atcagaagtg ggcgggtgac atcacgtact
      421 tgcgcacgga cgaaggctgg ttgtacctgg cggtggtcat tgacctgtgg tcccgtgctg
      481 tcatcggctg gtcgatgtcg tcgcggatga cagcgcagct ggcctgcgat gcgctacaga
      541 tggcactctg gcgacgtaag cgcccggaaa atgtcatcgt gcatacggac agaggcgggc
      601 agtactgttc ggcagactat ctggcgttac tgaagcggca taatctgcac ggtagcatga
      661 gtgccaaagg atgttgttac gacaatgcct gcgcggaaag cttcttccac tcgctgaagg
      721 tggaatgtat ccacggggaa cgccttatca gccgggaaat catgcggatg acggtgttta
      781 attatatcga gtgtgattac aatcggtggc gccgacacag tgcctgtggc ggactcagtc
      841 ccgaacaatt tgaaaaccag aacctcgctt agggccgtgt ccacattacg tgggtaggat
      901 caaactacag ttcacttaca ccgcctctca gcccggtgcg caccagaaaa tcattgatat
      961 gcccgtgaat ggtattgggt gctgtgccag tgcccgtatc acgggcgctg gcctcaacag
     1021 taagacttct atttattaat ggctcggttc attacgttca tccagacttc gcattcgcca
     1081 gcgacacttt tccaaaactt gtcagactta aggtttattg cgacatcttt tggtattatc
     1141 tttatatgcc agacaggatt tgccaatgca tctgtgtgaa taaagaatcc attattatcc
     1201 cacgcgctgt accctgcttg gttgttgatt gcactggctt gagataaagc tgtgaaatta
     1261 cccattacgc tgttagtgga accaatgaca ttaaccttgc taatgccaga tacgttatat
     1321 ctccatgatt tgatgttatc aaagtttagt gtggcggaat ttttccctga ctttagttcg
     1381 attgttttgt tttttgtgtt gatttttatt tcgtgctctt cgccaccaaa ttttgcataa
     1441 taaatataaa tcccatcgtt cttttcattt agatacttga tgtatttaga cttatcgaac
     1501 tcaagctttg atacgcaata cccaatgaaa attaaaaaag ccccgagcac ccagtattga
     1561 agagcgggtg ttttgctgaa aatcattggt agtgagccgc aaccaataga taaagcacct
     1621 gcaatatacg ctattctttt taatgcgtaa tatattttca cttttaaatc tccgttttag
     1681 aaatcatatt tcctggctaa aaatacactg ttaattctta ttgtgcaatt gaattataca
     1741 ctgagttatt taagattaca tttaaatatg atgtaatctt ttggctcgga atttggtgat
     1801 aaatatcagt ttaatgaaaa ttgtttatat tcaaatcgaa cctaatggct ttgaggtgat
     1861 gataccaact tactgattta gtgtatgatg gtgtttttga ggttctccag tggcttctat
     1921 ttctatcagt tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
     1981 tgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac
     2041 ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggtgt
     2101 tggatgccgg gcaaccgccc gcattatggg cgttggcctc aacacgattt tacgtcactt
     2161 aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga tgtgattgtc
     2221 tgcgctgaaa tggacgaaca gtggggctac gtcggtgcta aatcacgtca gcgctggctg
     2281 ttttacgcgt atgacaggat acggaggacg gttgtggcgc acgtattcgg tgaacgcacg
     2341 ttggccacgc tggaacgtct tcttggcctg ctgtcggcct ttgaggtcgt ggtatggatg
     2401 acggatggct ggccgctgta tgaatcacgc ctgaagggaa agctgccacg ttatcagcaa
     2461 gcgttatact cagcgcattg agcgacataa cctgaatctg agacaacatc tggcaaggct
     2521 gggacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcggaca
     2581 ctatctgaac ataaaacact atcagtaagt tggcagcatt acccatatgt cgctgggcca
     2641 actcttctgg tttctgggtt ttttatcgtg gattacaagg ccgtcatctg ataatgcccc
     2701 taatggggca gatctctagg atagtagcaa tacagcaagg aacttcttga gatcctttct
     2761 gttgcgtcgt aatctcatgt cctgcaaaca aaaaaaccac cgctaccaac ggtggttcgt
     2821 ttgccggaat caagagctac caactctttt ttccgaaggt aactggcttt cacggagcgc
     2881 agataccaaa actgtccttc cagtgtagcc gcagtcaggc caccacttca agaactctgt
     2941 agaagcactc tactactctg ctccgtgaat cctgttacca gtggctgctg ccagtggcga
     3001 taagtcgtgt ctttccggat tggactcaag acgatagtta ccggataagg cgcagcggtc
     3061 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct tcccggaatt
     3121 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     3181 gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt
     3241 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     3301 gggggcggag cctatggaaa agcgccccgg cggggcgtgg ccttttcgcc cttgtcctta
     3361 gctttcgtta tgcttaccgt tacgttatct gctcatcttc ctccgctcgc cgcagactca
     3421 tgaccgaacg gagtgagcga atgagcgagg aagcggaaaa ccatcagaac gtacagtaag
     3481 cactttcgcg ccggggcctt catgaacaaa caacaaaaga ctgccgttaa catggctaaa
     3541 ttcatccagg atcagtccct gcttcttctg gacaggctta atgagctgga tctggatcat
     3601 gaggctgatt tctgcgagaa actccatgag gatgcggaac gcctttaccg aagcctgtca
     3661 gcgcgcctga atgagcagca ggacggtgcg taatggctcc gttctgggta ttaccggagt
     3721 aataccggca tcgccttaat tttcattgtg ttagcgctta acattattac cccggtaata
     3781 cccagaatcc ctgtattttt tcttttttat ttatttttca tgaagttgcg atgaaaatcg
     3841 cagctgcgtt aggtgtatga ccattttgag gttgaaaaat aatcaaccat ggcgggtgat
     3901 ttacgcaggt taaggggcgt cggtgacaaa aaaacgtcat taacgacctc gttcatgctc
     3961 gcatgaacgg attatttttc cggactacag gtaaactaca gaaagactac aaaagctgat
     4021 gacttaatcc tgatttttag tgcgatgact acagataaac tacaaaataa ctacaggatt
     4081 acctcagatc cgttctctga accattcgta catccgaccc ccttaccggt tttacactga
     4141 gcactgatgc tctgaggtcc cttatgacga aataacagaa aacagcgctg aacatggcta
     4201 aattcatcca gaatcagtct ctgctcctgc tggagaagct taacgagctt gatctcgatg
     4261 ctgaggccga cctttgtgag aaactccacg atgatgcgga gcatcttttc cgtacactgt
     4321 cctcgagact ggatgaatta caggatggta actgatggcc agagtgtcta ttagtgaagc
     4381 agcacggctt gttaaggtta gtcgccctac tatttacaag atgattaaca gtggtaagtt
     4441 aagttacacc tctgttgtaa agcacgggaa gggtattaaa gttattgata catcagagct
     4501 cattagagtg tttggttctc ttgatggtgt aatcgatgct gtaaaatatg atgtaaaaag
     4561 tgacgcagaa tctacaggta ttaacagcgt tggtttacat ggtttacaac atagaatcgc
     4621 tttacttgaa gctgaaaacg atggtcttaa aggggctgtt aaggctcgag atgagcatat
     4681 tgattccctt cgtcaggcta tgcagcttct ggagcacaag cacgaacctt cttcaccacc
     4741 ccattccccg tggtggaaat tctggaaaaa atcctgatcc cgccatgctt gagttttcct
     4801 ctccggccat gccggggcgt tcgtgaacct tcccaaaaat cgccagatct ctgaacgctc
     4861 ctcacactaa aaatatccac accccacgca aaattaaatt tttgcagata actcattaat
     4921 atcagtcagt tggttgttat taaattacgc agtattacct tctgtaaggt caatccaagt
     4981 cgtcggtgat tcgtgtacga cttgccgacg actcgtcata gagtcatcga cgactcactc
     5041 cacactatca gtaacttatg acgacttgac tctagagtcg aatcgtcgat ttggcgtttt
     5101 tttgaccgtg gtgactctag agtcaagtca cgagtcgaat cgccggtgaa tcgtgtggtg
     5161 agtcgtccgg cgacttgcca taacgtcatt gccggccgtc gacgaacccc attcctgaac
     5221 gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcttc acggccacgc aataaattgc
     5281 gtccccgttc accgttttcg ggtaagaaga acatcggaaa caggacttac cccggctgaa
     5341 tggcgtgaat ttccgcgcta tgcacttgcg cgcatactca tgcatgccgt aaaaacagag
     5401 cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct gttttaccgg tttcccgtca
     5461 gaaacgccct gaggccgttt tcgcggtgcg cgtaatgcga cgttatggta aatcggtagc
     5521 gcgaagctga tgtggtagat ttgctggctg ttgatgtttg ttcttatgga gagattgtgg
     5581 ctacagatgc acaagtaaaa cagattaatg cgctcatcaa aaaataccct gactcgtgct
     5641 ctatttgcca tcaggaatac gatgaggatt acgttacgta tacggttttc ggatatgaca
     5701 aaaaccagaa gatgcaggtt acaactggat gttgcgcgcg aacgcttacc gaattggtgt
     5761 tgttaggtgt ttgcggatgc tttgatccag atgagcgtga cgagatcatg gaaaatcacc
     5821 caatggcgaa gaaatttttc agcaactaga cctgactatt accgaagccc gctctttgcg
     5881 ggcgtttggg ttcgtgcaat gagggtttat gttaaatcag ttctttttct atcaggtact
     5941 ttagcgctgc taaaagataa gatgagttga aggttatatc ttgatagctg gatgggctca
     6001 tgcttcctgt tttcttatac tcttcaaaga aattatttac gtagacggct tctaacttgc
     6061 gtgtatttcc tttcgaattt accacataga gttttccgtt atcactaaac atcgtgaact
     6121 cagtctttct ggcctcagtc atcagttctt tttcaacttg aaattcctca attaggatct
     6181 catagaacgg ttttgaggtg ttcaggataa tctctttttt ttcatgtgta ttttctattt
     6241 gattcaaata catctgtatt gattccctga ttaaataaga gcgttttttg cctgttttag
     6301 ctacagcgtt atctagtttt tgcagtaatt cgtcaggaag tgatactgct atcatttgga
     6361 tgcctctata gttaacgtct attcgattca tctaatttta tataaaatta gatgttcgtc
     6421 aatgggtgag gcctcttttt taatcaacta tgtgtgggta atactgccaa cttactgatt
     6481 tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc atttccatca gatgtccttc
     6541 ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc actgccggac atcagcgcta
     6601 tctctgctct cattgccgta aaacatggca actgatctta cccacgaata gtggacacac
     6661 gactaagtga gtaaactctt aaccagaggt gactcatgac aaaaccagca tcaaccagta
     6721 agaagccccg caaacagtac acgcctgaat tccgcaacga agccctgaag cttgcggaac
     6781 gtatcggtgt tgctgccgct gcccgggaac tcagcctgta cgaatcccag ctctacgcat
     6841 ggcgcagtaa gattaacaat gcccgcagct cttccgaacg tgagcaggaa atgtccgttg
     6901 aaattgcccg tcttaagcgc caactggcgg aacaggcaga ggagctggcc attctccaaa
     6961 aggccgcgac atac
//
