LOCUS       NZ_CP056715             3708 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter asburiae strain RHBSTW-00542 plasmid pRHBSTW-00542_5,
            complete sequence.
ACCESSION   NZ_CP056715
VERSION     NZ_CP056715.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148650
            Assembly: GCF_013740195.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00542
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 92.8048455495562x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013740195.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:53:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,089
            CDSs (total)                      :: 4,971
            Genes (coding)                    :: 4,860
            CDSs (with protein)               :: 4,860
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 35 of 111
            Pseudo Genes (incomplete)         :: 67 of 111
            Pseudo Genes (internal stop)      :: 24 of 111
            Pseudo Genes (multiple problems)  :: 12 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056715.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00542"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:61645"
                     /plasmid="pRHBSTW-00542_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3648..3708,1..284)
                     /locus_tag="HV233_RS25345"
                     /old_locus_tag="HV233_25355"
     CDS             join(3648..3708,1..284)
                     /locus_tag="HV233_RS25345"
                     /old_locus_tag="HV233_25355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_039025506.1"
                     /translation="MSEKRNKMLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPAR
                     TGKLPSLSPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            493..>927
                     /locus_tag="HV233_RS25625"
                     /old_locus_tag="HV233_25360"
                     /pseudo=""
     CDS             493..>927
                     /locus_tag="HV233_RS25625"
                     /old_locus_tag="HV233_25360"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            962..1453
                     /locus_tag="HV233_RS25355"
                     /old_locus_tag="HV233_25365"
     CDS             962..1453
                     /locus_tag="HV233_RS25355"
                     /old_locus_tag="HV233_25365"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_136070821.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESRDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1460..1675
                     /locus_tag="HV233_RS25360"
                     /old_locus_tag="HV233_25370"
     CDS             1460..1675
                     /locus_tag="HV233_RS25360"
                     /old_locus_tag="HV233_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2873..3019)
                     /locus_tag="HV233_RS25695"
     CDS             complement(2873..3019)
                     /locus_tag="HV233_RS25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071809246.1"
                     /translation="MHEPPVQSDCCACSGNYRLESKPERHDKAPLAAATGKRVYKRLLM
                     QSS"
                     /name="replication initiation protein"
     Typing          3381..3554
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          complement(2851..3457)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.803"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..278
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.391"
                     /coverage="86.0"
                     /evalue="1.15e-49"
     Typing          275..1767
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcttgagcg ctgcgacggc aggccgctgg cggcgtggat gcggcagacc tgcctggacg
       61 aaaagcctgc gcgcaccgga aagctcccct cactctcgcc ggcgctgctg cgccagcttg
      121 ccggtatggg caataacctg aaccagatag cccgccgtgt taacgccggc ggcggcaccg
      181 gccatgacag ggtacagatt gtggcggcgc tgatggccat cgatgccggg cttgagcggc
      241 tgcggcacgc cgttcttgag aaaggaacgg acgatgatcg ttaaatttca tccacgcggt
      301 cgcggcggtg gcgccgggcc ggtggactat ctgctgggta aagaccggca gcgtgaaggc
      361 gccagcgtcc tgcaggggaa gccggaagaa gtccgggagc tgattgacgc gtcgccttac
      421 gtgaagaaat acacctccgg cgtactgtcg tttgcggagg ccgatctgcc tcccggccag
      481 cgggaaaaac tgatggccag cttcgagcgg gttctgatgc ccggactcga taaagatcag
      541 tacagcattc tgtgggtgga gcacgcggac aaggggcgtc tggagctgaa ctttctgatc
      601 cccaataccg aactgctgac cggcaggcgg ctccagccgt actacgaccg cgccgaccgg
      661 ccgcgcatcg atgcttggca gaccatcgtg aacggcagac tggggctgca tgacccgaac
      721 gcgccggaaa accgccgggc gctggtcacg ccgtccggac tgccgaaaac gaagcaggag
      781 gccactgaag ccatcacgcg cggactgctg acccttgctt cgtccgggga gctgaaaaac
      841 cgtcaggacg tcactgaggc gctggaacgg tcaggttttg aggtggtgcg caccacgaaa
      901 agcagtatca gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagccatc
      961 tatgagcagt cttttaacgc tggcgaagga cttagagcag caatcgaaag cgcagaagca
     1021 gagtaccggc gagatgctga aagccgcatt cagcgagcac gagcagtctg tcagagcgga
     1081 actgagcgca agcgcgagga gaatcagcga cgccatcctc gcacacgagc agagtatgag
     1141 cgaggcgatg gagaaaaacc ggcggagcgt actgcggact gccgggcgga catggttcac
     1201 catcctgatg gtgtcagcgc tgctgatcgg cacgagcggg agcatactgt ggtggcaggg
     1261 gcagcagata accgacaatt acacgcatct tcgtcagcag gaagataccc tggcgaaaat
     1321 gactgcccgg acgtggggag tgcgctatca ggagagccgc gacggccgga ggtttcttat
     1381 cctgccgccc gggatgcaaa ccgaagccat cccctacgac gggacgacat ggatccgcct
     1441 gaaacaggag tagcggaaaa tgacagagct ggaaaagcag ttgctgagcg cattagagca
     1501 gctacagcag gactactcgc aaaggctgga cgaatgggag agcgccttcg cggaatggcg
     1561 gagcatgtgt gggcttatgc aacgggagaa cgcggtgctg aacgagcgcg tgtcggactt
     1621 gagcacgcag gtgctgagtt taagcgagca gctgcgccgc ttgtccggga actgaatgcc
     1681 atcgagctcc ggcagaggga gaatgagtat cagaaaacac tggagctgga gcgccagccg
     1741 cagaaaacct accgcgggcc gacactttaa accatccagg cagctgacaa cccgcacgaa
     1801 gaggggctct tatttctccc gctaaatagg ttcgtgatca aaaaggtcac gacttattca
     1861 ggagcgggaa atgaagctgt taaatcttat tgaatcattg cttaactgcc tgtatctggc
     1921 ggcggagctg tgggactggc tggagaagca tttgtcggtt tttaaggcat tggcgcggat
     1981 tttagtgtga ctttaacatc catgcatctg gctgcatgac tatgcaccag tgtaatattc
     2041 agcggctgcg tttctggtgt tttttcgtta tgtctgtcgc cactttctcg cgtctggcac
     2101 cggaaacgca gccaaccccg tttcagcggt gcgcccaacg gatgttatgg taaatggtgc
     2161 ctaattaacg ttgacgttta catcagcagg actttgataa ttaggtcgct gtaatccact
     2221 atcgccaacc gataagcatt ggaatatgat gcttatatca tcccctggga gcgagctaaa
     2281 cgtagagtga tccatgttct tcacaaggat ggtcttgcct tgattaaggc aatattggtt
     2341 cgcttctgtt aacgcagctt tttttgctcc agcctgtccc cttacaggtg cagcatgaac
     2401 agatatggaa taagtatctg gccccatggg taatacgcca cttgaattaa cgcatcctgt
     2461 aagggaaatc attactgctg caaagcaaat gtatttcatt actctaatat cctatatttt
     2521 tatttttcta tttattcaaa tggttatgct ggtttgttgg gtgattgtgc atcatccaac
     2581 tatacgattt tttatgcttt attttcaaag ggttttgtcg gtgtgagttt ttgactggta
     2641 ctgttgtttg gtatcggttt tactttgatg tctgtcggtc atcgaagatg aacctcttga
     2701 gatcctttcc gttgcgtcgt aatctcttgc tctgaaaacg aaaaaaccac ctggggaggt
     2761 ggtttttcga aggttcagta agttggggaa cttctgaacc gtggtaacag ggtgtacaag
     2821 accgctgcca ccaaattcgt ccttccagtt tagccgtagt ttggctctga cttcaagaac
     2881 tctgcatcag taatctcttg tacaccctct taccagtggc tgctgccagt ggcgctttgt
     2941 cgtgtctttc cggcttggac tcaagtcgat agttaccgga acaggcgcag cagtcggact
     3001 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg gaaccgagtg
     3061 tcaggcgtgg aatgagataa acgcggccat aacagcggag tgacaccggt aaaccgaaag
     3121 gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta tctttaagtc
     3181 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     3241 ggagcctatg gaaaaacggc tctggcgtgg ccttatcgct tccctgttaa gtttcttcct
     3301 ggcatcttcc aggaaatttc cgccctgttc gtaagccatt tccgctcgcc gcagtcgaac
     3361 gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg
     3421 ctgacgcacc ggtgccgcct ttttcctcct gccacatgaa gcacttcact gacatccgca
     3481 ttcgtgccaa catagtaagc cagtatacac tccgctagcg ctacgtgact ggttcagggc
     3541 tgcgccccga aacccgccaa aagccactga cgcgcctgcg gcttgtccaa ccccgcgccg
     3601 accgggaaag gctttcccgc ccgtcccggc gttatcagga ggccggtttg tcagagaaac
     3661 ggaacaagat gctcaccatg tgggtgacgg agggcgagca ccggcggc
//
