LOCUS       NZ_CP056714             6149 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter asburiae strain RHBSTW-00542 plasmid pRHBSTW-00542_4,
            complete sequence.
ACCESSION   NZ_CP056714
VERSION     NZ_CP056714.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148650
            Assembly: GCF_013740195.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00542
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 92.8048455495562x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013740195.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:53:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,089
            CDSs (total)                      :: 4,971
            Genes (coding)                    :: 4,860
            CDSs (with protein)               :: 4,860
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 35 of 111
            Pseudo Genes (incomplete)         :: 67 of 111
            Pseudo Genes (internal stop)      :: 24 of 111
            Pseudo Genes (multiple problems)  :: 12 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056714.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00542"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:61645"
                     /plasmid="pRHBSTW-00542_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5976..6149,1..207)
                     /locus_tag="HV233_RS25305"
                     /old_locus_tag="HV233_25315"
     CDS             join(5976..6149,1..207)
                     /locus_tag="HV233_RS25305"
                     /old_locus_tag="HV233_25315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106106982.1"
                     /translation="MKYYKHDSYIRRKYTREQIDRFFEISENDFTTYNFEKFFDDVVID
                     ILNKREENKDRSFAGYVYFMLDKFNIKNKRIQSYEVYFMTKKQIEKNLKDFEYEVSKSY
                     VRKWIYRWIYYYAVFVKSEKRK"
                     /name="hypothetical protein"
     gene            212..352
                     /locus_tag="HV233_RS25310"
                     /old_locus_tag="HV233_25320"
     CDS             212..352
                     /locus_tag="HV233_RS25310"
                     /old_locus_tag="HV233_25320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181553548.1"
                     /translation="MIGNNNDDYDEYLFDDTVEVSLSCEDDEDFLKDQEQEKKICFTEN
                     D"
                     /name="hypothetical protein"
     gene            517..858
                     /locus_tag="HV233_RS25315"
                     /old_locus_tag="HV233_25325"
     CDS             517..858
                     /locus_tag="HV233_RS25315"
                     /old_locus_tag="HV233_25325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040219661.1"
                     /translation="MAFNIEKYNKVKKELEQKANLLNRMEQEMKAEERKKDAARKIKVG
                     AELIRIVRENNIETLDFANDISLLVGALTNNLILKRIHDQRDFLRNEGDKRLNEWKLKN
                     NKKRSKTNE"
                     /name="hypothetical protein"
     gene            851..1264
                     /locus_tag="HV233_RS25320"
                     /old_locus_tag="HV233_25330"
     CDS             851..1264
                     /locus_tag="HV233_RS25320"
                     /old_locus_tag="HV233_25330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040219659.1"
                     /translation="MNNIRPNPNPRKEGTADMMNRLNFYDTSFTCLHFLMIDFDNDMQE
                     MKRKKDFHPLMLLNKHDRASLDLEILNIIRISLAGKMLNIQSDELLNADLYFQKQDELD
                     IGEDARIIAYVFRDFVKQRIEAAIKTIEEDKNI"
                     /name="hypothetical protein"
     gene            1261..1506
                     /locus_tag="HV233_RS25325"
                     /old_locus_tag="HV233_25335"
     CDS             1261..1506
                     /locus_tag="HV233_RS25325"
                     /old_locus_tag="HV233_25335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040219657.1"
                     /translation="MNIAAFLGFVAKAKPDVDDGGGSLIPAAMGFFIVAFLVAIIASVV
                     SSAFRSAKNSADVSINNAKKYHVALIKEQYRRKGGG"
                     /name="hypothetical protein"
     gene            1508..1963
                     /locus_tag="HV233_RS25330"
                     /old_locus_tag="HV233_25340"
     CDS             1508..1963
                     /locus_tag="HV233_RS25330"
                     /old_locus_tag="HV233_25340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040219655.1"
                     /translation="MYYLICSLFITIFFIVCMLSVIYAAEIYQWQHYNAYKFKRWLKSG
                     SIKKDEEQEKIKREVKKMTIDNILRLLKKYKIDFDANELVKNDFNIKMKYYKLILAEKE
                     RLKENKRLDEAVKQKIKIETDTFDAEKFQKEAEERFKAFMKNRNKNK"
                     /name="hypothetical protein"
     gene            2775..4466
                     /locus_tag="HV233_RS25335"
                     /old_locus_tag="HV233_25345"
     CDS             2775..4466
                     /locus_tag="HV233_RS25335"
                     /old_locus_tag="HV233_25345"
                     /inference="COORDINATES: protein motif:HMM:NF013603.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_181553549.1"
                     /translation="MLSEERLSAIRAKVNRADGSTNKKSATLSSVSTNGDRITERKKES
                     FKTANSFIIRGKIEENETLTKRGERSLNCAQQRYIVVVNGKAHTVFTHRCRSRHCQNCQ
                     RIKAFVWQEKMKEIFPDLISKYPNHKYLFITFTVKNPKLKNLKSVLICMSRAYAKMFQR
                     KAFKKWFVGDIRSTEITRGNSGDDEAHPHFHALICVHPTYFKGANYMSADVWGQNWGEC
                     LESEFKKEGLEYNASDYPKGFPRVDIRLAKSADRKTDITNQNISEHGEQLINYVLKYSV
                     KGSDLLKNGKADAWFWEFDKQVKNIRMIAASGDIKKSLAKLERDQFDYYKEQEKIEKLI
                     SDDVNFKADKAVFKKEDEYDYYLESKKDKFQYIIEAIEAKAYNYQCYYKALLSDHLLAF
                     RSGIDGIIENFRKINKAKTREDFEILEAAEQELVKIQCERIERLQFICDALVRVGLYKK
                     MSDFVYMNIHTNEKTVIVKNNIVDILKAAEEKLLNDFKQSFMRLESAKTQDVFNTIVEK
                     VNEKSALIKLVRNEILAAGAYAYDADNLEYIDLETNERLEVIATIE"
                     /name="protein rep"
     gene            complement(4483..5637)
                     /gene="mobQ"
                     /locus_tag="HV233_RS25340"
                     /old_locus_tag="HV233_25350"
     CDS             complement(4483..5637)
                     /gene="mobQ"
                     /locus_tag="HV233_RS25340"
                     /old_locus_tag="HV233_25350"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_181553547.1"
                     /translation="MAIFHMSAQTISRSKGHSSVAAAAYRHGEKITDEHTGEIHDYSKK
                     KGVSDGVVLIPDGADKRFLKPEYLWNTIEKSEKRKDAQLAREFNIALPVEMTNEQKKAL
                     AIDFCNENFVKNGMIADIAFHKLDSDNPHFHVMLTTRKLTPDGSGFGQKVREWNSKEQL
                     ECWRKSWADTANEHMQRAGIDARIDHRSLKDQKAELDAIPSPTVEQQAQAISLDRPPTI
                     HRGHSGNRERLERFDALQEVKASQEAKALNHIEINTLKPSPPAPASSPTASLPDAKQGG
                     KKAGSAAATVSGSNINLADETTDKNLHLTEAQREELEDAENKEAAAKKKAYLTGGAVVP
                     DFSETNKIQSKSEFKNRFNKMKNAINKEDEEEENTYKINRPKLK"
                     /name="mobQ"
ORIGIN
        1 gcggggtatg tatattttat gttagataag tttaatataa aaaataaacg cattcaaagt
       61 tatgaagtgt attttatgac taaaaaacag attgagaaaa acttgaaaga ctttgaatat
      121 gaagtttcta aaagctatgt gagaaagtgg atttaccgct ggatttatta ctatgccgtt
      181 tttgtgaaga gtgaaaaaag gaaataaaaa aatgataggg aataataacg atgattacga
      241 cgaatattta tttgatgaca ctgtagaagt ttctttgagt tgtgaagatg atgaagattt
      301 tttaaaagat caggagcaag aaaaaaaaat ctgttttaca gaaaatgatt gagtataaag
      361 atgaaagtac gcttttaggt agtttgtacg aaatatccgg cgtgtttggt tttactgttg
      421 ttttattgtt agggtttatt ctaacccttc catttttatt agtgattagt gttgtttcgt
      481 tttttattac aatgaaagaa aagaggggtt caaaaaatgg cgtttaatat agagaaatac
      541 aataaagtaa aaaaagagct tgagcaaaaa gcaaacttat tgaatagaat ggagcaagaa
      601 atgaaagcag aggaaagaaa aaaagatgct gcgcgtaaaa taaaagttgg cgctgaacta
      661 ataagaatag ttagagaaaa taatatagag acattagatt ttgcgaatga tatttcttta
      721 ttagtggggg ctttaactaa taatctaata ttgaaaagaa tacacgatca gcgcgatttc
      781 ttgagaaatg aaggcgataa aagacttaac gagtggaagc taaaaaacaa taaaaaaagg
      841 agtaagacga atgaataaca taagacctaa cccgaaccct cgaaaggagg gaacggcgga
      901 catgatgaac cgccttaact tctatgatac gtcttttact tgcttacatt ttttaatgat
      961 agattttgat aatgacatgc aagaaatgaa aagaaaaaaa gattttcatc ctttaatgct
     1021 tttgaataaa cacgatagag cgagtttaga tttagagata ttaaatatta ttagaatatc
     1081 acttgctggc aaaatgttaa atattcaaag tgatgagcta ttaaacgctg atttatattt
     1141 tcaaaaacaa gacgagttag atattggcga ggatgcaaga ataatagctt atgtatttag
     1201 agactttgta aaacaaagaa tagaagcagc tattaaaact attgaagagg ataaaaatat
     1261 atgaatatcg cagccttttt gggttttgta gcaaaagcaa aaccagatgt tgatgatggc
     1321 ggagggagtc tcattcccgc tgctatgggg ttttttattg ttgctttttt ggttgctatt
     1381 attgcgtcag ttgtttcaag tgcttttcgt tctgctaaaa actcggcaga cgtgagtata
     1441 aataatgcaa aaaaatatca cgtagccttg ataaaagagc agtacagaag gaaagggggc
     1501 gggtaatatg tattatttaa tatgtagtct atttataact atatttttta ttgtttgtat
     1561 gctttcagtt atttacgctg ctgagatcta tcagtggcag cattacaacg cttacaagtt
     1621 taaaagatgg ctaaaaagtg gaagcataaa aaaagatgag gaacaagaaa aaataaaaag
     1681 ggaagtaaag aaaatgacta tagataatat tttaagactc ctcaagaaat acaaaataga
     1741 ttttgacgct aacgagttag tgaaaaatga ttttaatatt aaaatgaaat attacaaact
     1801 tatattagca gaaaaagaaa gattaaaaga aaataaaaga ttagatgaag cagtaaaaca
     1861 gaaaataaaa atagaaactg atacatttga cgctgaaaag tttcagaaag aagcagaaga
     1921 acgatttaaa gcatttatga aaaatcgtaa taaaaataaa tagcctcgat ctcaacttgt
     1981 caaaaaaaat taaggagggt ccgccgaagg tggaaacctt catttttatt gacaactccc
     2041 cgataaaaaa acactttcaa tggtgaaagg agagcaaggc gagcctttcg cctgttaagc
     2101 cgcctttacg cccttctaag ccctttaccg tccctctgtg gtaatctctg gctgctggcc
     2161 tcctgtcgtt ccgggtgggt ttgttaagtg gtgggagtgc cagctcctgc agttgcgtcc
     2221 gggggcattt gacgccccgc ccttgccctt tggtttttgg gttttgggag tgagcgaagc
     2281 gagtgaccta cttcccttgt ttaataagaa acaaatcaga tttttttaac cccaaaaatc
     2341 ggcctgtaag ccttggtgtg tatggctttg ctcgaaaatc tattggtcaa tggcggtagc
     2401 taaaacgccc attttttaac caaaaaatac tcgcacgggt tcctgctgaa aatgggacgg
     2461 gtctgcataa aatcgccctt tcctctcctg tgttgggatg tgatctgcaa aaggtttcgg
     2521 ataaacggct gaaggtggga tgacaccacc taaaaccgcc tttttatccc tgctttttgg
     2581 gatgagggaa agcggagcgg cggaaaatgg gcgcaaaaat gggacaggtc tgcacttttg
     2641 ggcggtttta ggtgttcggg atgggcggct gaggtgttcg atttgtatgc aaagcgggtg
     2701 taagtgattg acagaaataa aaaacccctt agattggtag ttgcgacgct acacaaaaat
     2761 aaggggtttt ttatatgctt tcagaggaaa gattaagcgc aatacgcgcg aaagtcaata
     2821 gagcggatgg ctctacaaat aaaaaaagtg caacactttc aagcgtttct acaaatggtg
     2881 atcgaatcac agaacgcaaa aaagaaagtt ttaagactgc caatagtttt attattcgcg
     2941 gcaagattga agaaaatgaa acgctaacaa aacgcggcga acgttcttta aactgtgcgc
     3001 aacagcgtta tattgtcgtt gtgaatggta aagcgcacac tgtatttact catcgctgcc
     3061 gctctcgtca ttgccaaaac tgccagagaa taaaggcttt tgtatggcaa gaaaaaatga
     3121 aagagatatt tcccgatctc atttctaaat atccaaatca caaatacttg tttattacat
     3181 ttacagttaa aaatccgaag ttaaaaaatc ttaaatctgt attgatttgt atgtctcgcg
     3241 cttacgctaa aatgtttcaa cgtaaagcgt ttaaaaaatg gtttgtgggt gatattagat
     3301 cgacagaaat cacgcgagga aatagcggag atgatgaagc gcacccgcat tttcacgcgc
     3361 ttatttgtgt ccatcctaca tattttaaag gtgcgaacta tatgagcgca gacgtatggg
     3421 gccaaaactg gggcgagtgc ttagaatctg aattcaaaaa ggaaggatta gaatataacg
     3481 cttctgatta tcctaagggt tttccgcgtg ttgatattcg ccttgctaaa tctgctgata
     3541 ggaaaacaga cattactaat caaaatatca gcgaacacgg cgagcagctt ataaactacg
     3601 ttttaaaata tagcgtcaaa ggttcggact tgctcaagaa cggaaaggct gatgcttggt
     3661 tctgggagtt cgataagcaa gtaaaaaata ttagaatgat tgcggcaagc ggcgatatta
     3721 aaaaatcact cgctaaactt gaacgcgatc agtttgatta ttataaagaa caagaaaaga
     3781 tagaaaaact aatatctgat gatgtgaact ttaaagcaga taaagcagta tttaaaaaag
     3841 aagatgaata tgactattat ttagaaagta aaaaagataa gtttcaatat atcattgaag
     3901 cgattgaagc taaagcgtat aactatcaat gctattataa agctttatta tctgatcatc
     3961 ttttagcgtt tagaagtggt atcgatggca tcattgaaaa ctttcgaaaa ataaataaag
     4021 ccaaaacgag agaagatttt gaaatattgg aagcggcaga acaggagctt gtgaaaatac
     4081 aatgcgaaag aatagagcgt ttacagttta tttgtgatgc gctcgtgaga gttggtttat
     4141 ataaaaagat gtctgatttt gtgtatatga atatacatac aaatgaaaaa actgttattg
     4201 taaaaaataa cattgtagat attttgaaag cagcagaaga aaaactttta aacgatttta
     4261 agcaaagttt catgagactt gaaagcgcaa aaactcaaga tgtatttaat actattgttg
     4321 agaaagtaaa tgaaaaatcg gcactgatta aacttgtaag aaatgagatt ttagcggcgg
     4381 gtgcgtatgc ttatgacgct gataacctcg aatatataga tcttgaaact aacgagcgtt
     4441 tagaagttat agcgactata gaataatagc tcttgcgagc tattatttta gttttggtct
     4501 atttattttg taggtgtttt cttcttcttc atcttcttta ttaatcgcat ttttcatttt
     4561 gttaaatcta tttttaaact cacttttaga ctgtattttg tttgtttcgc taaagtcagg
     4621 aacgacagcg ccgcccgtca gatatgcttt tttcttcgct gctgcttctt tattttctgc
     4681 gtcttctaac tcttcgcgct gcgcttctgt tagatgtaga tttttgtctg ttgtttcatc
     4741 tgcgagattg atattagatc ctgaaactgt agccgctgct gaacctgctt ttttgcctcc
     4801 ctgtttagca tctggtaaag atgcggtagg ggaggaagca ggagccggag gcgagggctt
     4861 aagcgtgttt atttcaatgt ggttaagtgc tttagcttct tgtgacgctt tcacttcttg
     4921 aagcgcgtca aagcgttcta aacgctccct gttgccgctg tgccccctgt gtatggttgg
     4981 cggtctgtct aagctgatag cctgcgcctg ttgctccacg gtgggggagg ggatagcgtc
     5041 aagctctgct ttttggtctt tcagtgaacg gtgatcaata cgcgcatcga tgccagccct
     5101 ttgcatgtgc tcgttcgctg tgtccgccca gctcttgcgc cagcattcga gctgctcctt
     5161 gctgttccat tccctgactt tttgcccgaa cccggagccg tccggggtta gctttcttgt
     5221 cgttagcatt acgtgaaaat gtgggttgtc gctatcgagc ttatgaaaag ctatatctgc
     5281 aatcattcca ttttttacaa agttctcatt acagaaatct attgctaatg cttttttttg
     5341 ttcgtttgtc atttctacgg ggagcgcaat attaaactcg cgtgctaact gcgcatcttt
     5401 tcttttttcg cttttttcga tagtgttcca caagtattca ggtttcaaaa atcttttatc
     5461 tgcgccgtcg gggattaata ccacaccatc agaaactccc ttttttttgc tgtagtcgtg
     5521 gatctctcct gtatgctcat ctgttatttt ttcaccgtgg cgatacgccg ccgcagctac
     5581 gctactatgt ccttttgagc ggcttattgt ttgggcgctc atatgaaata ttgccattgt
     5641 tttttctcct actattggaa gcacatatat taaaaaataa gtaaaactat ttttgcatta
     5701 tcaaaaatca ataatgtaat atgagcataa agcgagtatt cattattatt ttttgattgc
     5761 agtaaatccc catttatggg gcgcacacat tcgaagaatg tataagtgcg ctattgtagt
     5821 ttttagcaaa actaaaatag ccgctacacg ttaacctttt gtctataaaa tcgcgctctg
     5881 aaaataaaaa tacatatgtc gcgctttact tgagtaaata aatatattac ttaaatatat
     5941 ataacagaaa acacaaaata aaaaagaggt gaaaaatgaa atattacaag cacgattcat
     6001 atataagaag aaaatacaca agagagcaaa tagatagatt ttttgaaata agtgaaaatg
     6061 attttactac atataacttt gaaaagttct ttgatgatgt tgttattgat atattaaata
     6121 aacgcgaaga gaataaagat agatctttt
//
