LOCUS       NZ_CP056713             8608 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter asburiae strain RHBSTW-00542 plasmid pRHBSTW-00542_3,
            complete sequence.
ACCESSION   NZ_CP056713
VERSION     NZ_CP056713.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148650
            Assembly: GCF_013740195.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00542
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 92.8048455495562x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013740195.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:53:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,089
            CDSs (total)                      :: 4,971
            Genes (coding)                    :: 4,860
            CDSs (with protein)               :: 4,860
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 35 of 111
            Pseudo Genes (incomplete)         :: 67 of 111
            Pseudo Genes (internal stop)      :: 24 of 111
            Pseudo Genes (multiple problems)  :: 12 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056713.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00542"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:61645"
                     /plasmid="pRHBSTW-00542_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(8499..8608,1..100))
                     /locus_tag="HV233_RS25265"
                     /old_locus_tag="HV233_25275"
     CDS             complement(join(8499..8608,1..100))
                     /locus_tag="HV233_RS25265"
                     /old_locus_tag="HV233_25275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032201350.1"
                     /translation="MTKFEAFKDQITNAAKASFPEWVTFEYENDFPGYNESFVYESVCA
                     IKKMGELEVRNNADRYFSVKFISA"
                     /name="hypothetical protein"
     gene            306..1709
                     /locus_tag="HV233_RS25270"
                     /old_locus_tag="HV233_25280"
     CDS             306..1709
                     /locus_tag="HV233_RS25270"
                     /old_locus_tag="HV233_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element ISKpn21 family transposase"
                     /protein_id="WP_001567368.1"
                     /translation="MSAESSGVFTLKEINRIKIIQDVIERRITTRRAAEHLGISDRQCR
                     RLLARYREGGPLGMASRRCGMRGNRQLPPGLADQALELIKTRYADFGPTLAREKLEELH
                     GLFLGKETVRRIMVRAGLWVPRKQRAARIPQPRYRRPCTGELIQIDGCDHDWFEGRGPA
                     CTALVYVDDATSKLMELLFVKSESTFSYFEATRRYIDKHGKPLALYSDKAGVFRVNNKH
                     ATGGDGHTQFGRAMHELNIQTICAETSPAKGRVERAHLTLQDRLVKELRLQGICSMEAA
                     NDFAEAYMADYNRRFGKVPRHDFDVHRAVEHDEDLGLIFTVREKRKVSKSLTIQYDKML
                     YLIEDSELSRRAIGKYIDVYHYPDGRKELRLNGTLLPYSTYDRLSEIDQGAIVDNKRLG
                     RTLEFISLVQSKRDNTRSQSIPAGDGPSRRRPKQEGKKSQRSLDNDDMLEALKQLQSRS
                     EDIFGKRAR"
                     /name="ISNCY-like element ISKpn21 family transposase"
     gene            complement(1738..2370)
                     /locus_tag="HV233_RS25275"
                     /old_locus_tag="HV233_25285"
     CDS             complement(1738..2370)
                     /locus_tag="HV233_RS25275"
                     /old_locus_tag="HV233_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001567369.1"
                     /translation="MLRYELTPNNAGFILWGDSEALNELHELIHYIVDESPLIKVKDGF
                     MLSLAYDIRKAREGNRRVEQHQYDQHDTYKLYGVELLWPLVLVQSSILRNSMGYIQTDK
                     NQLSVMYAFEYLIESALTESERTTSNDIMLTVKYASDSDFNFIEDNIDSRCCYFISLSP
                     EQRKKQLISIVRSFHSLWGKYAREKQDIKMLNEMNNTSWVWPDNINW"
                     /name="hypothetical protein"
     gene            2661..2849
                     /locus_tag="HV233_RS25280"
                     /old_locus_tag="HV233_25290"
     CDS             2661..2849
                     /locus_tag="HV233_RS25280"
                     /old_locus_tag="HV233_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2897..3124)
                     /locus_tag="HV233_RS25285"
                     /old_locus_tag="HV233_25295"
     CDS             complement(2897..3124)
                     /locus_tag="HV233_RS25285"
                     /old_locus_tag="HV233_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230090456.1"
                     /translation="MFGFSPGELMNLSPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(3177..6074)
                     /locus_tag="HV233_RS25290"
                     /old_locus_tag="HV233_25300"
     CDS             complement(3177..6074)
                     /locus_tag="HV233_RS25290"
                     /old_locus_tag="HV233_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_023307208.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEVMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            6169..6774
                     /locus_tag="HV233_RS25295"
                     /old_locus_tag="HV233_25305"
     CDS             6169..6774
                     /locus_tag="HV233_RS25295"
                     /old_locus_tag="HV233_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            8063..8443
                     /locus_tag="HV233_RS25300"
                     /old_locus_tag="HV233_25310"
     CDS             8063..8443
                     /locus_tag="HV233_RS25300"
                     /old_locus_tag="HV233_25310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032201349.1"
                     /translation="MKKITSLLLLGVFGLTGCAQNVSPQVAAQAQAPIYCENKQQCDAY
                     WTKAKAWIAMNSGWKIQMADETVIATYSPIGNSAILGYQVVKTPIGGERHEIVVQTACA
                     NMFGCIPNAIEQTAKLKNYIKN"
                     /name="hypothetical protein"
     Typing          7268..7757
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.316"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 atccaggaaa atcattttca tattcaaacg ttacccactc aggaaaagat gctttagcgg
       61 cattcgttat ctgatctttg aaagcttcaa acttggtcat ggatatatcc tatgtaagaa
      121 ccgggtggct cagagagatt ttaccacaga aagaattagc atgatgagat caccaattta
      181 ccataacatc cgttgcccgc accacctctg taatggctca atagtaatgt ccgcctgtgg
      241 ctcaattcag atgtccgcgc tatggtaagc ttcactggtc cgtttaaact accgggaggc
      301 atatcatgag cgcagaaagc tcaggagtgt ttactttgaa agagatcaac cggatcaaga
      361 ttatacagga cgtcattgaa cgtcgcatca caacgcgccg tgcggccgag cacctcggta
      421 tcagcgacag gcaatgccgc agacttcttg cccgttaccg tgaaggcgga ccgcttggta
      481 tggccagcag acgatgtggc atgcgtggta accgccagtt gccacccggg ctcgcagatc
      541 aggctctgga actgatcaag acgcgttatg ctgatttcgg tccgactctg gcgcgtgaaa
      601 agctcgaaga actccacgga ctgtttcttg gcaaagaaac tgtccggcgc atcatggtgc
      661 gggctggctt atgggttccc cgtaaacaac gtgccgcaag gatccctcaa ccacggtacc
      721 ggcgtccgtg tactggtgag ctgatacaaa tagatggctg tgatcacgac tggtttgaag
      781 gccgtggccc ggcctgcacc gcgctggtct atgttgatga tgcaaccagc aaactgatgg
      841 aactgttgtt tgttaaatcg gagtccacgt tttcttactt cgaagccacg cggcgctata
      901 tcgataagca tggtaaaccg ctggcactgt acagcgataa agccggtgtt tttcgtgtta
      961 acaataaaca cgccacaggc ggagacgggc atactcagtt tgggcgagcc atgcatgaac
     1021 tgaacatcca gactatctgt gcagaaacca gtcccgccaa agggcgtgta gaacgagctc
     1081 acctcacttt acaggatcgt ctggtcaaag agctgcggtt acagggcatt tgttcaatgg
     1141 aggctgcaaa tgacttcgct gaggcctata tggctgacta taaccgccgt tttggcaaag
     1201 taccgcgaca tgattttgac gtacaccgtg ctgtagaaca tgatgaggac ctggggctta
     1261 ttttcactgt tcgtgaaaaa cgtaaagtct caaaatcgtt gacgatacaa tatgataaaa
     1321 tgttgtacct gattgaagac agcgaactga gtcgccgtgc aataggtaaa tatatcgatg
     1381 tgtatcacta tcctgatggc agaaaagagc tgcgcctgaa cggtacgcta cttccctact
     1441 ctacctacga ccgactgtca gaaatcgacc agggcgcgat tgtcgataac aagcgtcttg
     1501 gccgaaccct ggagtttatc agtctggtgc agagcaagcg ggataacacg cgctctcagt
     1561 caattcccgc tggagatggc ccttcccgac gacggccaaa gcaggaaggg aagaaatccc
     1621 agcgctcact ggataatgat gacatgctcg aagcactcaa acagcttcag tcacgttcag
     1681 aggacatttt tggtaaaaga gcccgctgat ggtactcact ggccggacag tggttgctca
     1741 ccagttgata ttgtccggcc agacccatga tgtattattc atttcgttca gcatctttat
     1801 gtcctgcttt tcacgggcat acttacccca taatgaatga aaagaacgaa caatactgat
     1861 taactgcttt tttctttgct ccggagatag gctgataaaa tagcagcacc tgctgtcaat
     1921 attatcctca atgaaattaa aatcagagtc tgatgcatat tttactgtta gcataatatc
     1981 attcgacgtt gttctctcag actctgttaa tgctgattct atcaggtatt caaaggcata
     2041 catgacagac agctggtttt tgtctgtctg aatataaccc attgagtttc tgagtattga
     2101 ggactgtacc aggaccagag gccataaaag ctcaacacca taaagcttat atgtatcatg
     2161 ttgatcatac tgatgttgct caacacgacg attaccttcc cgtgctttac gaatatcata
     2221 ggcaagggat aacataaatc cgtctttaac tttaatcagt gggctttcat ccacgatgta
     2281 atgaatgagt tcatgtaatt cattcagggc ttctgaatct ccccacagta taaaacctgc
     2341 attgttcggc gttaactcgt atcgaagcat gggctcaatc atcctgttgt ttcagtcgga
     2401 ggaaagtcta tcagtaaaga gaatgagaga tcaaagtggt cattttaatt gagctggata
     2461 acggacattt caattgagcc ttgacaacct caggcgccct cagggcgttt ctgaggactc
     2521 cggcctgaaa catacacctt tctgtgccca tatcgagaaa aaacgcccct gatgatttct
     2581 gcgtacctgc atggctatgc actcaagtga atgaagtaag atttcagtct gcgatcacca
     2641 taaaaccggg aaaaagccgt atgacaaaac aggaatcagc cgcactgaac atggcaaaat
     2701 ttatccgggc gcagtcgctg ctgctgctcg aaaaactcga cgtgctcgat cttgatgacg
     2761 aggcagccga ctgtgagcga atgcacgaac aggcagaagc gctttatcag aagctgagcg
     2821 cacgcttcgg tatacaggac ggcgaataaa gagacgaaaa gaacgctcac ttagacgaaa
     2881 agcgtatcca gggcaactat gtatatccac cgtgtacata gtcatgcctg acgctttcga
     2941 gcaggtcgag catgtaggaa cgacgagctt gattggtatc ggacacaata tggcgatacg
     3001 cctcaaacat aacagcaccc gcatcaccgc ccgaacggcg aagcatagcg gaaatcatcc
     3061 gcacaaagag cagacgttca tcggggctaa ggttcataag ttctcctggt gaaaaaccaa
     3121 acatgaaagc acccctctat taaggggtcg gttccggctg agggcgaaat gacaccctaa
     3181 gctttcggtt ccttgggcca aagatattcg ccagtcagta gaatgtgcgc ccagcccaat
     3241 ggggatatgt ggggaagaaa ttcaggggga acatccaacc cttcgttccg ccgctccgtg
     3301 acggcatgac caagatggac ggtattccag taaatgatca ccgcagtcaa taaattgagc
     3361 ccagcgattc ggtagtgctg cccctctgtc gtgcgatcgc gaatttcccc ctgcctcccg
     3421 atacggagcg catttttgag cgcatggtgg gcctctccct tgttaagacc gatctgagca
     3481 cgccgctgca tgtccgtatc caggatccac tcaataatga aaagggtccg ttcaatacga
     3541 ccaacttcac gaagcgcaac tgcaaggttg ttttgtcgtg ggtaagaagc gagcttgcgc
     3601 aggagttggc tgggcctgat tttgccagcg gtcatcgtcg cggcacaacg gaaaatatca
     3661 ggccagttcg caacgataag atcctcccgg gcttttccac ctaccaactt gcgtaactcc
     3721 ctgggggtcg tatcgggatt aaatacgtac aaccgcttcg atggcagatc cctgattcgc
     3781 agaacgagat tgtagccgag caggctactg gctccgaaca aatggtcggt gaatcctgct
     3841 gtatcggcat actgttcgcg aacatggcga ccgacctcgt tcatcagtag tccatcgaga
     3901 atatacggtg cctcgctcac ggtcgccggg atcgactgac aagcgaatgg cgcgaactgg
     3961 tcgcttacgt gagtatacgc tttgaggccg ggaacagaac catatttggc attgaccatg
     4021 ttcatgactt cgccatgccg cgctgtcggg aaaaactgac catcgctcga tgctgacgtg
     4081 cccatccccc agacgcgtga catcggcagt ttaccctgcg cggccaccac aattgccaat
     4141 gcctggttca tggcttcgct ttcaacatgc cagcgggcaa ggcgtgagag ctgccagtaa
     4201 tcatgcgtgt ttgtagcttc cgccatctta cgcaggccca gattgagccc ttcagcgagc
     4261 aggacgttga gcagaccgat ccggtcgcga catggagccc cggttctcag atgggtaaac
     4321 gcatctgtga aaccaagggc tgcatcaact tcaagcagca tgtcggtaat ccgaacggac
     4381 ggcattcggc gatacagatc cagtatgagt gcctcggcac catccggcac gtctgctgtc
     4441 aacctgtcga tccgcaacgt tccatcttct atgctaccgt gcggaatagt gccgttacgg
     4501 gcagcccggg ccagccgctt aagagcgatc gtgagtcgcg cctttctgtc tgccagccaa
     4561 tcctgtgggt tggaaggcac ggccagtttt gcattttcct gcgccgcgat catcggcacc
     4621 agtacctgct tgaggtcacc atagcggcgc gaatgagcga gccagacatc tccggaacga
     4681 aaagcatccc ggaggtgaaa gagtaccgcc acttcccaaa gacgggtatc tcctttttcc
     4741 tgagctcgta aatgacggtt ccatttggag ctgggccgca ggaaacgcct ttctggcgat
     4801 gcaacacctt tcatctctcc gatcgacaaa gctgctgcta ccaatggtcc ggcgaccggc
     4861 gcggcttcga gcttcagaca gcgcaacatg cggggcgcat aacgacgaaa gcgatggtat
     4921 ccctgcccga catatgcaag aggctcatcg gctagcgtgt tgctgagttg agtccctgtc
     4981 gctaccagtt gagcgagccg gtcccatgca accgaactgg cgacagccat ctccagcggg
     5041 gttccgtcac tgcgggcctc aagcaacgaa gctcccagcg cggtgaaggt acggatcgta
     5101 tccgtgagtg tggctttaga gccggaaatt gtttcgtcat gctggcgctt cgcttcccgc
     5161 caggtttttc ctacgatcct gtcatgggtt tcgactatgg catcagcaat cgccgcttcc
     5221 cactccacaa cacagacggc aaggatcgcc cagcggcggt ccgaagtgat gtcacgcaaa
     5281 ccgtcggtga agtagcgttc accctgccga cgcagccggg caatgcgatg ggcaggtatg
     5341 ctggccaaag cactatgatt gatattcagg gtacgcagaa attcgagcct gtcgagcaaa
     5401 cggttagcag cagccgagtt gttaccaacc tcgaagttgc gaagccagat gaaacgactg
     5461 atattgccgg cgagcatttc actcagaagt ttgtccaggt gatcgcgaac atccgctgtt
     5521 aaattttccg caatccgcgt ttcaatccgc cgctcagcgg cgaccagagc atccgcgcac
     5581 aagcgctcga ttgtcgatac tgcgggcaga atggtggaag tttcccgaca ccgcacaata
     5641 aaacgatgag caagatcctc gtttgatctg gcatcttcgg cctggccgaa agtccactcc
     5701 cgcagatcac gggcaccacg gcccgtgaag gtcttgtagc cgtaaatttc gcgcagcgtg
     5761 tccatgtgct gctgacgggt ttggcgccgt gtggcataag tgagaagcgc atcagccgga
     5821 actccaagct gagcaccgac gaaggaaagg acttcacgcg ggatcatctc accaggagcc
     5881 agtgcacggc ccggatatcg taaggcacaa agttgcaggg caaagccaat cctgttttcc
     5941 ggtctgcggc gctgcctaat gttttccagg tcatcatcgc ccagcgtgta gaacttcagt
     6001 agcgacagtt cgtccgtggg cagatcgaac agcgctgctc gctgccgttc ggtgaaaata
     6061 tggcgtcgtg acatacaaat tcgtcccttt tgaagtatag tctgttttgg acaacagcca
     6121 gcccatataa atcagggcgt tccgatacaa aaatccagga gggttcaatt gggacatcgt
     6181 gccgccattt actgccgggt ttcaacagcg gatcagtctt gtgaacgcca ggaatttgat
     6241 ctgcgagcct tcgccggccg tgccggctac gacgtggtgg gaatatttaa ggaaacaggt
     6301 tcaggaacta aactcgaccg ggccgagcga aagaaagtcc tggcgcttgc ccagtccaga
     6361 caaattgatg caatcctggt cactgagctt tcccggtggg ggcgctcgac gctcgatctg
     6421 ctcaatacgc tacgtgaact ggagaactgg aaggtttccg tgatagccat gaatggaatg
     6481 gcgttcgatc tttcgtcgcc gtatggacga atgctggcga cgtttctttc cggcattgcg
     6541 gagtttgagc gggatctcat cagcgagcgg gtcaagtcag gccttgctgt tgcgaaggca
     6601 cgtggtaaga ggcttggtcg tcaggccgga gtgcgaccaa aatcagaccg acttttgcct
     6661 aaggtggttg cgatgagggc cgagggacgc agctatcgct ggatcgcacg cgagctcggt
     6721 atcagcaaga ataccgtcgc tgacatcgtg caacgacaca gagctaacgc ttagggtgtc
     6781 atttcgccct cagccggaac cgacccctat taacgtcagc tgtgaacgct tcattagaag
     6841 aaaataggta aacccgggaa aaagatccaa acgttaacat cacaggtaaa cagaaacgtc
     6901 gggtcgatcg ggaaattctt tcccggacgg cgcggggttg ggcaagccgc aggcgcgtca
     6961 gtgcttttag cgggtgtcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt
     7021 atacaggcta aacttcagag atgccaagga agggacagga tatgttttgt agcaccgcca
     7081 ccacggtggc gatactccaa aacgcctggt cagggcacag ccccgacacc ccagagccga
     7141 ctgtaggtca ctgggcgaac gcggcggaat gatgtcggat gacgtcattt tgcggcgtta
     7201 gccctatcct gcatcgtata aaaaaatgca gctatggcgc gtacggtgcc aaagataagc
     7261 ccggagatat tccgcttcct cgctcactga ctcgctccgc tcggtcgttc ggctgcggcg
     7321 agcggtaccg gcttactggc ggggcggaaa tttcctggca tcttccagga agaagcttaa
     7381 cagggaagca agaaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag
     7441 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact taaagatacc
     7501 aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     7561 gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg
     7621 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     7681 gccttgtccg gtaactatcg acttgagtcc aacccggaaa gacacgacaa cgcgccactg
     7741 gcagcagcca ctgataactg tttagaggag ttagtcttga agttatgggc cggttaaggc
     7801 taaactggaa ggacagattt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     7861 agttgatctc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgtttacag
     7921 agcaggagat tacgcgctgc tcaaaaggat ctcaagaaga tcatttttct tttgcaacct
     7981 aaccacacct actttcttct ttcctacgta tagataatgt taaaatcagc acataaaaaa
     8041 caataaacaa cagaggctta aaatgaaaaa aataacttct ctattattac tcggagtctt
     8101 tggcttgacc ggctgcgctc aaaatgtcag cccacaagta gcagctcaag ctcaagcgcc
     8161 tatatattgc gaaaataagc agcaatgtga tgcttactgg actaaagcaa aagcttggat
     8221 agcaatgaat tcaggctgga aaatacaaat ggcggatgaa acggttattg caacgtactc
     8281 acctattggt aatagtgcaa ttttaggata tcaagtggta aaaactccaa ttggagggga
     8341 aaggcacgaa attgtcgttc aaacagcctg cgccaatatg tttggctgca ttcctaatgc
     8401 catcgaacaa acagcaaaat taaaaaacta cataaaaaac tgatgattat attcatgaaa
     8461 gcccacgttt atcgggcttt ccgaaacaat caaacatgct aggccgatat aaacttcaca
     8521 ctaaaatatc tgtccgcatt atttcttact tctaactcac ccattttttt aatagcacag
     8581 acggattcat aaacaaagga ttcattat
//
