LOCUS       NZ_CP056710             2519 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00564 plasmid pRHBSTW-00564_4,
            complete sequence.
ACCESSION   NZ_CP056710
VERSION     NZ_CP056710.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148653
            Assembly: GCF_013747035.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00564
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.683505864976x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013747035.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 05:20:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,575
            Genes (coding)                    :: 4,494
            CDSs (with protein)               :: 4,494
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 34 of 81
            Pseudo Genes (incomplete)         :: 50 of 81
            Pseudo Genes (internal stop)      :: 18 of 81
            Pseudo Genes (multiple problems)  :: 17 of 81
            Pseudo Genes (short protein)      :: 1 of 81
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056710.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00564"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00564_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            550..803
                     /locus_tag="HV236_RS23575"
                     /pseudo=""
     CDS             550..803
                     /locus_tag="HV236_RS23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(892..1167)
                     /locus_tag="HV236_RS23335"
                     /old_locus_tag="HV236_23345"
     CDS             complement(892..1167)
                     /locus_tag="HV236_RS23335"
                     /old_locus_tag="HV236_23345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181522288.1"
                     /translation="MNANGFKKFEHDSPYGRFGFRQNLPVCIQHHGSGIPSGIPSGIPS
                     GTPEPDRNNGHKQVSLIITKSHQGSGRKTKHGLPTVNGNAEERFTG"
                     /name="hypothetical protein"
     gene            complement(1474..1659)
                     /locus_tag="HV236_RS23340"
                     /old_locus_tag="HV236_23350"
     CDS             complement(1474..1659)
                     /locus_tag="HV236_RS23340"
                     /old_locus_tag="HV236_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2025..2252)
                     /locus_tag="HV236_RS23345"
                     /old_locus_tag="HV236_23355"
     CDS             complement(2025..2252)
                     /locus_tag="HV236_RS23345"
                     /old_locus_tag="HV236_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1345..1427
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="96.0"
                     /evalue="9e-28"
     Typing          complement(149..677)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.385"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 tctcttgccc tgtaaacgaa aaaaccaccc tggcaggtgg tttttcgaag gttaggtaat
       61 cctggcagat cccctaaccg tggtaacagt cttgtgcgag acatgtcacc aaatttgtcc
      121 tttcagtgta gcctcactaa ggccgccact tcaagaactc ttgatacatc tctcgcacat
      181 cctgtttacc aatggccgtt gccaatggcg attagtcgtg tctttccggg ttggactcaa
      241 gttgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
      301 ccatcctgga gcgaactgcc ttcccggaac tgagtgtcag gcgtggaatg agaaaccgcg
      361 gccataacag cggagtgaca ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg
      421 agccaccagg gggaaacgcc tggtatcttt aagccgcatc gggtttcgcc accactgatt
      481 tgagcgtcag attctgtgat gcttgtcagg ggggcggagc ctatggaaaa acggcttcgc
      541 cgcggcctta tgccttcttc gctaagtatc ttcctggcat cccctaggaa atttctgctc
      601 cgtccgtaag cccgtaccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      661 gaggaagcgg aatatatcct gtatcacatt ttctcctgac gcgttttctt tcattttccg
      721 cgcctgtctt atgtggcagt tatgctatgc gttactgcgg tgttacattt ttagccagta
      781 tacactccgc tagtgctccg tgactggtcc ggcgctgcgc ccggaacccg cctgtaccgg
      841 ttcagcagcc gttccggcct gactgcaatt tttttttttc atccctgccc gctaccctgt
      901 aaacctttct tctgcgttgc cgttaacagt aggtaagcca tgtttggttt ttcttccgga
      961 gccctgatga ctcttagtga tgatgagcga cacctgcttg tgtccgttgt ttcggtctgg
     1021 ctccggcgtg ccggagggga tgccggaggg gatgccggag gggatgccgg agccatgatg
     1081 ctggatgcat accggcagat tttgtctgaa accgaaccgg ccgtaaggac tgtcatgctc
     1141 gaatttcttg aatccgttcg cattcattac atttcgtcct gagcattttc tgtatttctg
     1201 accatactta aacgaggcgg ccgtcacgga cggtggtcgt cgttccatga tgctgttatg
     1261 gacgagtgag agtaatctaa tctaacttga cttaccttcc gggcagctcg cccggttttt
     1321 tttgcctgtt cccggggaac cactccggac aggatgtgca tttgtaatac cgcgcacgcg
     1381 ccggcatcac aaatacttag gtcagggctg ctccccgaca ccccgccatc gcctcacggc
     1441 cgcgcactga attgcgcacc cgttcaccga ttttcacgca ttttcctcgc ctatctgtgc
     1501 gttcagcatg gcgtaaagcg cttcggcctg atcgtgcagg tgctcacagc agccggcagc
     1561 ctcatccaga tccatctgct cgagccgctc aaggaggagg agtgtctgtg cgcggataaa
     1621 tttggccata ttgagggcgg cggattcctg tctggtcact ggtgattttc cccgtctggt
     1681 gatgattttt gcgtgaaaag aggatggcgt cattctggtg catagtcatg catgccgtta
     1741 aaatttaaca ggcgcgtttc tgacgggttc cggggtggtt tgttgtggtt tttgtcatgg
     1801 tttcgtcagg aacgcgctga acgcgtctca ggcggtgcgg gcaacggatg ttatggtaaa
     1861 ttcttgggtg cttgattata aaataccgca cttcctctta tgtcttgtgc tttttagaca
     1921 accctgaaag cttttagctt tggtgatgca atttgctggg attttggcgg agaaccacag
     1981 gtaaagaaaa aggccacatt agcggccttt ttcggagaag atgctcagtg cggaatttcg
     2041 gagacttctt taacgtcggt tttattgatc tgctgtttga caccattcgc gtctgtatag
     2101 ccaagcaggc ctgagtctga ttctttaggt ttgccgtcgc tgacaatagt gcgaccgtca
     2161 ttggtgtgta tggcatagct tgtgcgcgtg cagccggtga gggctgaaag cgcaactgcc
     2221 gctgcgaaca ctgaaacaaa aatctttttc aaagccaact ccttttatcc atatcagttt
     2281 tggtcactaa ccatctaact ataggtcatt ttttatgtga aaagaggctt gatggtgggc
     2341 tgtatatcga gttaggcgcg aggtgctatg gtcaaagtgt ggtgtcaggc ggggaggagg
     2401 cttatatgag atgtaatccg gccgatacat ataccaagat gatttaagta ttcctctttg
     2461 tgaggttgga tgtctaattt ataaaggatc ttcttgagat cctttttttt cgcgcgtaa
//
