LOCUS       NZ_CP056709             4921 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00564 plasmid pRHBSTW-00564_3,
            complete sequence.
ACCESSION   NZ_CP056709
VERSION     NZ_CP056709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148653
            Assembly: GCF_013747035.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00564
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.683505864976x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013747035.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 05:20:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,575
            Genes (coding)                    :: 4,494
            CDSs (with protein)               :: 4,494
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 34 of 81
            Pseudo Genes (incomplete)         :: 50 of 81
            Pseudo Genes (internal stop)      :: 18 of 81
            Pseudo Genes (multiple problems)  :: 17 of 81
            Pseudo Genes (short protein)      :: 1 of 81
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00564"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00564_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            177..500
                     /locus_tag="HV236_RS23310"
                     /old_locus_tag="HV236_23320"
     CDS             177..500
                     /locus_tag="HV236_RS23310"
                     /old_locus_tag="HV236_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            709..>1143
                     /locus_tag="HV236_RS23570"
                     /pseudo=""
     CDS             709..>1143
                     /locus_tag="HV236_RS23570"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1178..1669
                     /locus_tag="HV236_RS23320"
                     /old_locus_tag="HV236_23330"
     CDS             1178..1669
                     /locus_tag="HV236_RS23320"
                     /old_locus_tag="HV236_23330"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1676..1891
                     /locus_tag="HV236_RS23325"
                     /old_locus_tag="HV236_23335"
     CDS             1676..1891
                     /locus_tag="HV236_RS23325"
                     /old_locus_tag="HV236_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2805..4091
                     /locus_tag="HV236_RS23330"
                     /old_locus_tag="HV236_23340"
     CDS             2805..4091
                     /locus_tag="HV236_RS23330"
                     /old_locus_tag="HV236_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     Typing          4813..4921
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="99.0"
                     /evalue="1.61e-48"
     Typing          complement(4342..4888)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          491..1989
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatagtaagc cagtatacac tccgctagcg ctacgtgact ggttcagggc tgcgccccga
       61 aacccgccaa aagcctgacg cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc
      121 tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc agagaaacgg aacaagatgc
      181 tcaccatgtg ggtgacggag gacgagcacc ggcggctgct tgagcgctgt gacggcaggc
      241 agctggcggc gtggatgcgg cagacctgcc tggacgaaaa acctgcgcgc accggaaagc
      301 tcccctcaat ctcgccggcg ctgctgcgcc agcttgccgg catggggaat aacctgaacc
      361 agatagcccg ccgtgttaac gccggcggcg gcaccggcca tgacagggtg cagattgtgg
      421 cggcgctgat ggccatcgat gccgggcttg agcggctgcg gcacgccgtt cttgagaaag
      481 gaacggacga tgatcgttaa atttcatcca cgtggccgcg gcggtggcgc cgggccggtg
      541 gactatctgc tgggtaaaga ccggcagcgt gaaggcgcca gcgtcctgca ggggaagccg
      601 gaagaagtcc gggagctgat tgacgcgtcg ccttacgtga agaaatacac ctccggcgtc
      661 ctgtcgtttg cggaggccga tctgcctccc ggccagcggg aaaaactgat ggccagcttc
      721 gagcgggttc tgatgcccgg acttgataaa gatcagtaca gcattctgtg ggtggagcac
      781 gaggacaagg ggcgtctgga gctgaacttt ctgatcccca ataccgagct gctgaccggc
      841 aggcggctcc agccgtacta cgaccgcgcc gaccggccgc gtatcgatgc ctggcagacc
      901 atcgtgaacg gcagactggg gctgcacgat ccgaacgcgc cggaaaaccg ccgggcgctg
      961 gtcacgccgg ccggactgcc gaaaacgaag caggaagccg cagaagccat cacccgtgga
     1021 ctgctgagct tagcctcgtc cggggagctg aaaagccgtc aggacgtcac tgaggctctt
     1081 gaaggtgcag gttttgaggt ggtgcgtaca acgaaaagca gtatcagcat tgccgaccct
     1141 gacggggggc gaaacatccg actgaaggga gccatctatg agcagtcttt taacgctggc
     1201 gaaggactta gagcagcaat cgaaagcgca gcagaagagt accggcgaga tgctgaaagc
     1261 cgcattcagc gagcacgagc agtctgtcag agcggaactg aacgcaagcg cgaggagaat
     1321 cagcgacgcc atcagcgccc acgagcagag tatgagcgag gcgatggaga aaaaccggcg
     1381 gagcgtgttg ctgacggccg ggaggacatg gctcaccatc ctgatggtgt cagcgctgct
     1441 gatcgccacg agcgggagca ttctgtggtg gcaggggcag cagataaccg acaattacac
     1501 gcatcttcgt cagcaggaag ataccctggc gaaaatgacc gcccggacgt ggggagtgcg
     1561 ctatcaggag agcagcgacg gccggaggtt tcttatcctg ccgccgggga tgcaagccga
     1621 agccatcccc tacgacggga cgacatggat ccgcctgaaa caggagtagc ggaaaatgac
     1681 agagctggaa aagcagttgc tgagcgtatt agagcagcta cagcaggact actcgcaaag
     1741 gctggacgaa tgggagagcg ccttcgcgga atggcggagc atgtgtggtc ttatgcaacg
     1801 ggagaacgcg gtgctgaacg agcgcgtgtc ggacttgagc acgcaggtgc tgagtttaag
     1861 cgagcagctg cgccgcttgt ccgggaactg aatgccatcg aggagaaggc tgcactggag
     1921 tatcagcgtc agaacgagct ggagctgacg ctccgacaga agcagaagag tttcgatggt
     1981 ccgaagatga ggtgacagcc cgcacgaaga agggctcatc gcgttcctgc taaataggct
     2041 gactaccgga atggtagtga cttatcccgg aggaagtgat gaaaattctc aacgttattg
     2101 aagccctgct gaactgtttt tacctggtga tggagctgtg tgagttgccg gagaggcatt
     2161 tgccggtttt taaggcgttc gcgctgattt tagtgtgact ttacgctgca tgcatctggc
     2221 tgcatgacta tgcatcagtg taatatccag cggctgcgtt tttggtgttt tttcgtcatg
     2281 tctgtcgcca ctttctcgcg tctggcaccg gaaacgcagc caaccccgtt tctgcggtgc
     2341 gggcaagggg tgttatgtta aaagccgagg tctggcggat acggaaaaaa ttatgcccgc
     2401 ggcaatccgg ccgatactta aattatcctt cacaaaggat gtgctgcgat ccaggcagtt
     2461 gagtgttttc cggctcccgg atgggagcca tttttttgcc ttctgatgcc tgttaatgca
     2521 tgcggagctg cgctggttct tccaagattg atagacgcca gcttctctgc tggagccggc
     2581 ggatttgccg taaatatgaa cattttctta ttgttcagcc cgctaaagat ggcgggtttt
     2641 ttacgcgtga aatccggagc cggacatcat gccggcatgg ctgaatagcg ggctttttcc
     2701 cgccctgtcg cagtaatata taacgctaca taaggagtag ccgataacca tggcagtact
     2761 tacattatcc gggcaatctg attcatatgt tttcacttaa aaatatgttc tccctgaacc
     2821 tccagcgatc agccatttcg tatgacgcaa tttgtcagaa taagcctgtc attgagttca
     2881 gtccggaggg ggtgataaat aaagccagcc cgctttttct ctccactatg gggtacaggg
     2941 ctgatgaaat aatcggtcac caccatcgta tattctgccc gccctcgctg gtaagttcac
     3001 cggaatacag ccagttctgg cagcgccttg ccaggggaga aagctttagt ggcaagtacc
     3061 tgcggctggc aaagggaaat cgttccgtat ggcttgaagc cagttatatc cccgtttctg
     3121 acaggcgcgg ccgtgttatc agagtcatta aaattgctgc cgatatttct gagcgcgtgc
     3181 attctgctct tgagcaggaa gcagttgtaa atgccataaa ccgttctatg gccatcatca
     3241 ccttcaatcc tgaagggatc gtgcttgaag caaatgaaaa tttcgtcaat gccactggct
     3301 ataagcggga tgaaatcata ggtaagcatc atcgtctgtt ctgtgccgag acgctttaca
     3361 aaagtgatga ataccgacat ttctgggaga gtctgaatca aggtgagttt ttttctggtc
     3421 tgtttccacg ccttaaccgt cagggagacc ctctgtggtt ccgtgcaact tataaccctg
     3481 tctttaacag tgatgggcag ctttataaaa ttgtgaaatt cgctacagat gttacggctg
     3541 atgtcctgcg aaaccagaga gagcaagagg ctgctgtgca tgcatgggat atggccgtgc
     3601 agacccgaga aagtgcgcag aacggtgcca atgttattga aaatagcatc cttatgattg
     3661 acagaattgc gcaggggatg ggggctgtct ccaccgatat ctcacggctc aacaatcagt
     3721 ctgaaagtat tgacgatatg gtggaaacca tccggaagtt tgccatgcag acaagactta
     3781 ttgcactgaa tgccgcgatt gaagcagcaa gggctggcgc atccggaaga agttttgcgg
     3841 ttgttgccgc agaagtgcgt aatcttgcgg cgagcgtcag tagcgcgact gaagaaattg
     3901 agcaagtcgt ggccagcaat agtcaactgg ccaaggatgt tctctgtggc attgaaaaca
     3961 gcctgatgaa cactcgggaa ggggttacac tcatgcgcga agcgggtgaa gttatgacca
     4021 gcattcagag gaatgctgca ggggttgaga ctgcggttaa ggatgtcgcc atttcagtta
     4081 aagccggata ggccatggtg catatcccca tatctgtcga acatcacaga tgatcttctt
     4141 gagatccttt ccgttgcgtc gtaatctctt gctctgaaaa cgaaaaaacc acccggcaag
     4201 gtggcttttt cgaaggttcg caagagttga cgctcttttg aaccgcggta actggcttgg
     4261 aggagcacag tcaccaaaac tgtcctttca gtgtagcctt atcgcacccg aaacttcaag
     4321 actctcctcc tctaaacctg ttaccagtgg ctgctgccag tggcgctttg tcgtgttttt
     4381 ccggattgga ctcaagttga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
     4441 gttcgtgcat acagtccagc ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg
     4501 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     4561 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     4621 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
     4681 tggaaaaacg gcttcgccgt ggccttatcg cttccctgtt aagcttcttt ctggcatctc
     4741 ccaggaaatt tccgccccgc ctgtaagccg gtaccgctcg ccgcagccga acgaccgagc
     4801 gcagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgct
     4861 ccgctgctgc atttttctcc tgccacatga agcacttcac tgatattcgc atccgtgcca
     4921 a
//
